FastQCFastQC Report
Sat 31 Dec 2022
SRR5849162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134191
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGC7410.5521979864521466No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTCAAAGATCTCGTATGCCGTCT6680.49779791491232644RNA PCR Primer, Index 47 (95% over 22bp)
TTCCATGGGTAATTGTGAGTCAATTAAACCTCTTTTCTTCATAAATTACCCA1970.14680567251156934No Hit
CAAAATGGGAAGTTATGAAAATAGTTAAGTGGAAATTTGGGACTTGAAATGT1890.1408440208359726No Hit
TCCCGCGGGGATCTACTTGCGGGGGCCAACCATGGTGAACTTGGGTCTGTCC1700.1266850981064304No Hit
TCTTTTGGGATAAATGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGC1610.11997823997138407No Hit
ACGGGGGGCACCAGTAGTTTTTGAGAGTTCAGCTTCTTCCATGTCTATTCCA1600.11923303351193447No Hit
CACAACGGGTGCTTTATTATTTTAGTTTCTTTGGGTGCCAGTTCTCTTGTTT1580.1177426205930353No Hit
CGTACAGGGTACACAGTGATTCTGAGGTTTATTTCAATTATTAGAACTGAAA1550.11550700121468653No Hit
ATCTACGGGTATTGAATTATGTACAACATTTTCATTTTTTATGCTTCTTGAG1550.11550700121468653No Hit
AGTCAAGGGATGTAAATTTAAATATTGCTACTTATTTTTTTTCTTTTCTGAA1540.11476179475523693No Hit
CGGTGCGGGCAATTGATGAGGGCTTAAACAAAAGCTTTGGCAGTATTCAGTT1530.11401658829578735No Hit
TCGTGAGGGTAGTTCAGAAACCAGTTTTCAAAACTTCCTTCATGTAGTAAAT1530.11401658829578735No Hit
CGCAGTGGGGAACATGTCATCATTCCCCGGATTGAACGTACTCTTGCTTATA1530.11401658829578735No Hit
GATGTGGGGAGTTGCAAACATGGAATATATTGATCCAACTGTATAAATAATC1520.11327138183633775No Hit
AGCTCGGGGTTTGCAACAAAGCTTACCTGCCCGAGCAAGTACTTGCATGCCT1520.11327138183633775No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1520.11327138183633775No Hit
TGATACGGGGAACATCCTTGAACATGCATCCCTGTTTACATGTACAAATATA1490.11103576245798899No Hit
TAGCATGGGATGGGAACTCTCTACTCTTCATTCCATTTTGTTGTGAATCTAA1490.11103576245798899No Hit
ACGCCTGGGAACCTTTCTTCATTTGTTCATGTTCTACTTGATGTGGTTATTG1470.10954534953908981No Hit
AGTTGCGGGGCACGCGTGAGACTTCTCCGCCGCAGACGCCGCCGCGATGCGC1470.10954534953908981No Hit
TCCGCGGGAAGATGGGGTGGGGGGCATATTCTTTGAATAAAGAAGACAAAAT1460.10880014307964021No Hit
GAGGAGGGGCTCTTCCGATCTTGTATGGGGAAACCAAGTCAGCAGAAGAAAT1460.10880014307964021No Hit
CGACTTGGGCAGCCATCGAAGGACACTTGGGTTGTTTCCACCTTTTGGCTAC1450.10805493662019061No Hit
TTGGCGCGGGATTGGCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAG1450.10805493662019061No Hit
TTGGGTGGGCTCCATATGTCAGAGCCTCTCTGGGATGAAGTTCAAGCCAGAA1440.10730973016074104No Hit
CCAAAAGGGGAATTCGAGGAAGATGATGTGAATCTTCCAATTTTATCTATTT1420.10581931724184185No Hit
TTTTATGGGATTTCAAGAGAAAGAAAAATAGCCTTTTCTTTATAGCCAGAGT1420.10581931724184185No Hit
CCGAGAGGGGAAGAGAAAGGATGGTTGCATCTATACTGAACATGTATTGAGT1420.10581931724184185No Hit
ATCAACGGGCGATCTAGCAACGGGTATGCTGCTTTTTGGGATACTAAGAAAA1400.10432890432294267No Hit
GGAAATGGGACTGGCAAAATACCTTTAATTTTATGTGCCACATATGTATAAT1400.10432890432294267No Hit
ATTTTAGGGAGATGGGCTTTTGATGCACTGTTGCCAAGGAAGGCTTTTTTCT1390.10358369786349307No Hit
GGTTATGGGAATGCCATGAAATAATCTTTGTCTTCTGTTCAGTTGTGATTAT1390.10358369786349307No Hit
CAATAAAGGGGAGCTGAAAAACTTACCTACTGGGGACTGTGCTCACTACCTG1390.10358369786349307No Hit
TTCGATGGGGAGTTTATCTGAATAAATATCATTAATGCTAATCTCTGGATTA1390.10358369786349307No Hit
GTTTCTGGGTAGGGTGATTGAGGTTGGGAATAATGACATCGATGATGTAAAC1390.10358369786349307No Hit
TCATAAAGGGATTGTTAGAGATTTCATTTTACAACACAAAAATAAGCCTGTG1380.1028384914040435No Hit
TGATTGGGGACTCCCACCGAGGCCCTGCAACAGCACCAAGTCTATTTCTAGG1380.1028384914040435No Hit
ACATTGGGAGCTCTAGAGAAAAACAGAACACCTGATTCCAGTAGTAGTTAAG1380.1028384914040435No Hit
TAAAGAGGGAGGTTATCTAGATGTATGATTTACATGTATCCTACTTGTATCT1380.1028384914040435No Hit
GAACAAGGGATCATTTGTTACTCAAATTATTCTTTTTTGAAGTATCTTCCTC1380.1028384914040435No Hit
GTCTCCGGGAATAAATTTAAATTCAAATATTAACATTTCAATTTCCAGTTTG1370.1020932849445939No Hit
TCTTTGGGGAGCCAAGCAGCCCTTCAAGTTCTTTTATTTCTGGGTAACAGAA1370.1020932849445939No Hit
GCTGCTGGGGTATCCAATAAGGTGTTGGTATCCAGAATATTTAAGAAACTCA1370.1020932849445939No Hit
CATAATGGGAAGGACGGTCTGAAGAGCCAAGAGCTATTTGATGAAATTAGAA1370.1020932849445939No Hit
CCAGCTGGGCTCTGACATAGTACTCCCATGATGGCTTCCAGTGACACATGTG1360.10134807848514432No Hit
ATTAACGGGACATAAAATCAGTTAATTCAAATGAATGTTTTTCTTTGACTAC1350.10060287202569472No Hit
AAACTGGGTATTTGGTAAAATTCAGCAGTGAAGTGATCAAGTCCCAGGCTTT1350.10060287202569472No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)