FastQCFastQC Report
Sat 31 Dec 2022
SRR5849116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60246
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCGTCT7051.1702021710984962No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGC6491.077249941904857No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCTTATAC1310.2174418218636922No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCT1130.18756431962287953No Hit
AGCCTTGGGTGCTGAAAGAAATAGAGACATAAATAAATGGAATCACATCTCA1050.1742854297380739No Hit
AGTAATGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA990.16432626232446967No Hit
GGAATGGGATGAAGTAACTCGCCAATTATTCTTAGGTTAACACACTCTTTGC940.15602695614646617No Hit
TTGGATGGGAACCACATAAGTTGGGGAACATCTATTAGTCTGAAGCAATGGT920.15270723367526473No Hit
TAAAGGGGGACGCGCCGGAAGTGCATTTGCAGAGTGAGACAAAGCGGAGAAC920.15270723367526473No Hit
GATCAAGGGAATGCAGAAGGAATATGGATATATTTCTTTAAGTCTGCAGATT890.14772764996846263No Hit
ATGAGTGGGCGAGACTAGCCTGGGCAACGTGGTAAAACCCTATCTCTATGAA880.14606778873286194No Hit
AAACTAGGGGCAGGGTTTAAAAATCAGTAAAAATGGGAATGATTGAGCTAAA860.14274806626166053No Hit
GCTCCTGGGTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAG860.14274806626166053No Hit
AATAATGGGGGAGAAAGTGGCAACATTAATATAATCTTTTAATCATTTCTAA860.14274806626166053No Hit
TTAGCAGGGGATGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTG830.1377684825548584No Hit
TTGCACGGGGTGTTAATCAGATACAGAACTTCCAAGAACCAGATGGCCTTCA830.1377684825548584No Hit
ATGTGGGGAATTCTGGTCTAGAAGATAACCTTAGTACTTGGTAGAAATTTTG820.13610862131925772No Hit
ACTGCCGGGCAAGCAATCCACATACTTAAAAAGCCATCAAAGCTAAGAATAG820.13610862131925772No Hit
AACATCCGGGCACAAATTAAAGCAACTTTCTTTTTATTTAAAAATTGGTAAC820.13610862131925772No Hit
GCAACTGGCATCTTCATTTCAAATTAATTTTTGTGAGGACCCAGAGAAGTGG820.13610862131925772No Hit
ATTTCTGGGGACCCATGAGAAAGGAAAACTTACTGATGATAATTTTTAAATA810.134448760083657No Hit
CTGTTAATGGGATAGATGAGTTGCACACAAAGCCTAGTGTATTATGGAAGAC810.134448760083657No Hit
GGATATGGGTGAACACATACGTTTCAGAAAAATTTGTACATTAAAGTGCATT800.1327888988480563No Hit
ATAGCAGGGATTTTAAAACTGAAACAATAACCAAATAACATTGGATAATAAA800.1327888988480563No Hit
CACTGCGGGGTATTTTAAAGGAGAGAAAGCAATCAACAACTCCTTTGTTTTT800.1327888988480563No Hit
TGTGATGGGAATGCATATGAGGTGCTCAAATAGTTGCTGAGTAAATAAATGA770.12780931514125418No Hit
CACCATGGGAAGCGTACATATAAAATAGTTGTATATTATGACATTTTAAATC770.12780931514125418No Hit
CTAGGGGGAAAATAAAACTGAGGTGCAAAGAAACATCAGTGACAAATAGAGA770.12780931514125418No Hit
CTTATATGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC760.1261494539056535No Hit
GCAAAAGGGAGGGAACCACAACCCTTATTGTCCTGGGTGGACCAATCCAAAG760.1261494539056535No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.1261494539056535No Hit
GCAATGGGGGGAGACTAATGACTTTTTCCTTAATTTATTATCAAATTTGCCA760.1261494539056535No Hit
TATAATGGGGCTCTCGCTATAGCTGTTGTCCAGATCGTTATCTTCTCAGAAA760.1261494539056535No Hit
GAGAGGGGAAATCAAAGAACTGATGCACTATATAGAACGAATTTGGGTTTTT760.1261494539056535No Hit
TGTTGGGGGATTCAGAAATAGACTTCGATATATATGAACTTTTTCATATGTG750.12448959267005279No Hit
TGATAAGGGGGCCTTTCTCCGCTGGCAACGGCGCCGCTCCCCGCTCCTCCTC750.12448959267005279No Hit
CGGTTTGGGACACGTTTGAAACCAACTACTGTAGAAGATGAGGAATCTATTG750.12448959267005279No Hit
CTTGCTGGGATAGCTACATGTACAGATGTTCACATTTAAGAGTAATGCGAGT750.12448959267005279No Hit
ACACCTGGGAAGATGGTGGGGATGCCATACAACAGGACTATGTGATGATTTT740.12282973143445208No Hit
TGTAAAGGGAAAATGAAAATGTTTCATTTTTAAAGGGATGGTAAGAATTTTT740.12282973143445208No Hit
ACCTTCGGGGAAATCAGCAGGAGAGTAGAGGAGACACCAGCCCCACCAGGAG740.12282973143445208No Hit
AGACGAGGGACGATAGAATGTTACTGATGATTCAGGGCTATTTCTGAAGTCT740.12282973143445208No Hit
GCAAGGTGGGGAAAAGCTCACAAAATTGCTAGCCACAAATACTATACCCAGC740.12282973143445208No Hit
TTCATAGGGGTCACAGTTCTTCTTCAGATACTGATATTAATGAAATTCATAC730.12116987019885138No Hit
TTAACTGGGCAAGTGAAATATGAAAATGGTTTCCATAATAACAATTATTGAT730.12116987019885138No Hit
TGATGTGGGACCCCTTGTTGAGGGGCTTCTATTACTCTTATCTGAATTCTAC730.12116987019885138No Hit
CATTCCGGGTGGTCCATGACTTGATTTTTTTCATTTAATTTGCAAACCAGCC730.12116987019885138No Hit
AACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT720.11951000896325066No Hit
TATTTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC720.11951000896325066No Hit
TTGAAAGGGGACAAATGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCAA720.11951000896325066No Hit
GGCTATGGGAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAAC720.11951000896325066No Hit
TTCCATGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGC720.11951000896325066No Hit
TATTAAGGGATTCCAAAACATAATTGGAAAATAGAAACTGAGCCATTGCCAA720.11951000896325066No Hit
TCACATTGGGAACTGACTTTTATTCACTTGTTGTGACAAGTTTTTAATTACC720.11951000896325066No Hit
CACATAGGGTGCCTTTAGCTCTGTTTTATTAGACAGAGTTAAAGCATGTTGT710.11785014772764998No Hit
GCAAGAGGGTAAGGAGTTGAAAATACAATCAACATAGCAATGGAAAGCAATG710.11785014772764998No Hit
TTATATGGGAGTGCTATAACTCTCCTGCCCATGATAGTGTATTCTGTTTCAG710.11785014772764998No Hit
GATCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.11785014772764998No Hit
TACAAAGGGTCTAACGTAGGGCCAGAAACAGATGATGAAGAAGAAAAAAAAC700.11619028649204927No Hit
GATTAAGGGGGAACCATCTAATGAGGCAGGCTTAAACTCTATTTAGTTTCGT700.11619028649204927No Hit
TAACAAGGGTTGAAGTTTAAACTCGGTTATCTCTTCATGAGACTATTATGTT700.11619028649204927No Hit
GCGCTTGGGCTCCCCAGAAAAAAACGCATAATAGGCACATAAAAAGAATTTT700.11619028649204927No Hit
GTTTCTGGGACCTAAGGGAATTAGTTTTACACCTTCAGATAAAGACTGCTGA700.11619028649204927No Hit
GAAGATGGGACCCACCACCAGCCGTCCCGCAAGCAGCATCTGTCTGTCCGTG700.11619028649204927No Hit
CTAGAGGGTACATTTTTCATAACGACCTGCAAATATAAAACCTTTCTTTCTC690.11453042525644856No Hit
AGCCTGGGTGACAGAGGGAGATTCTGTCTCAACAAATAAACAATCCCCCCAA690.11453042525644856No Hit
AGGTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA690.11453042525644856No Hit
AATGTGGGGGGACAACCACACTGGGAGTGTGGGTCATAGAGTCTCAGGATCA690.11453042525644856No Hit
TCTAGTGGGATGAGCACCCCAGGCAGCTCAAGCTGAATAAATAGCTGCAGAC690.11453042525644856No Hit
ACAAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA690.11453042525644856No Hit
ATTGGCGGGAAGAGGGAGGCTGGTTCTGTTGCTCGTATCAATAGGTTATCTG690.11453042525644856No Hit
AACTATGGGCTCATGATCGATTACAAGTTTATGGAGGACACAAAGACATGAT690.11453042525644856No Hit
CAGCCTGGTGAAAACATCTGAAAATAAGTCTACGGACTGTACTCAATCTACA690.11453042525644856No Hit
GATCTAGGGATTAGTCTTGTTAGCGGTCTATCAATTTTGTTGATCCTTTCAA680.11287056402084784No Hit
GTTTTAGGGATTTTGAATAAAATGAATCACAATGTCTCTTTTCATCAAAAAG680.11287056402084784No Hit
GTGCTTGGGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAAC680.11287056402084784No Hit
GTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA680.11287056402084784No Hit
ACTCCTGGGGCTCCACGGTTACCCCGGCTCTCCGCCCCTCCTTCTCGCGGCG680.11287056402084784No Hit
TTATCAGGGAGACAGTATTTCAAGAGAGTGGCAGGTCTGTTCCTGGTAAAAT680.11287056402084784No Hit
GCCTTAGGGGAGTGGCATTAGGGAGGGATTGTGAGAAATGACTTGTAAATAT680.11287056402084784No Hit
CGCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC670.11121070278524717No Hit
TGATAGGGGGAACACAATGCCACGCTTCTTCGACAATGAAGGCAGTACATAA670.11121070278524717No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAAACTCGTATGC670.11121070278524717No Hit
TGCCCTGAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC670.11121070278524717No Hit
TATTGAGGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGAT670.11121070278524717No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCTGTC670.11121070278524717No Hit
TAACGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG670.11121070278524717No Hit
TTAACTGGGGGTTAAAGACAGACTGCAGTGGTCTCATAAGGTTATCATGGAG660.10955084154964645No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCA660.10955084154964645No Hit
CGCTAAGGGGTATGTAAAAAATCTTACTTTAAGTAAACCTTACCATGAATTT660.10955084154964645No Hit
TAAGTAGGGGAAGGATGCACATGAAGAAATGGAGCAGGCTGTGGAAGAATGT660.10955084154964645No Hit
TGTGTCGGGATCTGAAGGACAAGGAATGCATCTAGAACAAGTCCCATGTTTT660.10955084154964645No Hit
GCTATCGGGATTTAGAGGAAGTAAGGTAGCAGGCAGTCCAGCCCTGATGTGG660.10955084154964645No Hit
GAATACGGGGTGATTTTGTTCATTTATTATTTTCTATCTTCACATTGTAATG650.10789098031404575No Hit
TCTGCAGGGTAGGCCTTCTCCAACCATCTGCCTTATGAGGTTCACCCATTTC650.10789098031404575No Hit
ACACTGGGGATATGACTCAAGTGTGAGTGGTCAATCATCTTTTGTGACATCC650.10789098031404575No Hit
TATCTGGGAGGTTTGCCATTAGGGAAGGAAAGAAGTAGAAAGCAACCTGAAA650.10789098031404575No Hit
GAGACAGGGGACCACTGCAACCACCAATAACAAAATGTATATTCTTTTTCAG650.10789098031404575No Hit
AAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA650.10789098031404575No Hit
ATCTGTGGGGGGGTCATTTATTTAGAGACACCTATACTTAGTAGGACATGTA650.10789098031404575No Hit
TCTTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC650.10789098031404575No Hit
GTAGGAGGGAGCTTCAGAAAACAAGATGCCATAGTAAATTGAGAATAACATT650.10789098031404575No Hit
AGCAGTGGGCTTCAGGAAAATGCCACTTCTGCTCATTGATGGGAAAAACCGA640.10623111907844503No Hit
CTAACAGGGTAAAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCT640.10623111907844503No Hit
CCAGCCTGGGTGACAGAGAGAGAGACCTTGTCTCAAAAAAACAAAACAAAAC640.10623111907844503No Hit
CGACATGGGAGATACCAAAATCCATGAATTTTCAAATCCCCTTTATAAAATG640.10623111907844503No Hit
CTCGATGGGACCTTAAATTCTTGACATGGATTAGAGAATAAATATATACATT640.10623111907844503No Hit
CGCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.10623111907844503No Hit
TTCAGAGGGAAGTTAAAAAGCTGCTGTACAGCAAAGGAGACAATCAACAAAG640.10623111907844503No Hit
GAGCTTGGGATTGTATTGCTTAATAGAATAAAGAAGTAGAAATTTAATTCCT640.10623111907844503No Hit
TGTTTGGGGGAAAAAAGTCATACTAATTTGCTTCTTTTTCAATGTAGTGCTT640.10623111907844503No Hit
GATCTAGGGGTGGGCTGTGTGTACCTTGGTTGGTGACTCAGTGAGAAGAAGC630.10457125784284435No Hit
CGGCTTGGGAGCCCTCAGTAAGTCATGAACCATAGAGAAGGTCATGGGGCCA630.10457125784284435No Hit
GCCAGTGGGGGCACATGTAAACTCACAGAGAGGAAGGGAACTGACATGAGTT630.10457125784284435No Hit
TGATTAGGGCTTTTTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA630.10457125784284435No Hit
CACGTGGGGAGAGATTCTGTTTGCTGTTGAGAATGTTGTTATTTGGATTTCT630.10457125784284435No Hit
TCGTTGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA630.10457125784284435No Hit
AATTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC630.10457125784284435No Hit
CTATCCGGGTATCTTGTTACATTTACTTTTTTAAATAAAGAAATTACAGAAG630.10457125784284435No Hit
TAATAGGGGGGAACTCTAGAAGATAATGGTTAGGCAAGTGATTTTTTTTTTT630.10457125784284435No Hit
GCTCACGGGCATTTTCCTAAGTGTTGGATAATTCCCTGACCTTTAAAAAGGA630.10457125784284435No Hit
GCTAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC630.10457125784284435No Hit
TAAGCAGGGGGAAAAAAATTTTAGCCTTATCTTAATCTGTCCCAACAGCAAT630.10457125784284435No Hit
GTCAAAGGGTTACTCAGTGATAAATGAATCTCTGAAAACAAATGCTTTTACA630.10457125784284435No Hit
TGTAGCGGGATGCCAGCAGCATTTTCCCTGCCCACACAGTTATGACAACCAA620.10291139660724363No Hit
GCGCAGGGGCAAAAAGAAAATTTACAGAATAAGCCAATTCCTGGTAGAGAGA620.10291139660724363No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.10291139660724363No Hit
GATGTGGGGCTGAAGGAAATCAAAGGAAATCTAAATAAGTGAAGAGATGTGA620.10291139660724363No Hit
TTGTCTGGGGTATGTATAATTCAACAATTTTAGATTATGGTGTAAGATACTC620.10291139660724363No Hit
CTGCCAGGGAACTATATAGATAAGGAATACATTTTAAGCAAAGATTTTTCAT620.10291139660724363No Hit
TACAGTGGGTATTTCTTCTAGTGTAATTTTTTTTAAAATTCATATTTGTGTC620.10291139660724363No Hit
GAAACAGGGACTTGGATGAAGCTGCTAGAATGGTTGTAAAGCTCTCTGAAAT620.10291139660724363No Hit
TATGCTGGGTCACTCAGAACTATTGATTCAAAGTTGGGTAGTGAATCAGGAA610.10125153537164293No Hit
GAACAAGGGGTGCCAGGACAGGTGTCCCTTCAGTCCTCTGGTTATGACCTCA610.10125153537164293No Hit
ATAGTAGGGGACCAGCATCACATTGGTAATGGTATAGTAGTTATCAAATCTC610.10125153537164293No Hit
TGCTTGGGGATTTCCATTTCTGGAAGTTGTAAGAGCTCACATTAAAGACAGT610.10125153537164293No Hit
TTATGGGACTTACTAAGAATCTCTTTAAATCAGAGTCAACCCATGGCATCTA610.10125153537164293No Hit
TTGTCTGGGCAATACATAAACATGACACAAGAGATTATAGCTAAAATAGAAA610.10125153537164293No Hit
ACAATAGGGGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGA610.10125153537164293No Hit
TGCTCACGGGCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATT610.10125153537164293No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)