FastQCFastQC Report
Sat 31 Dec 2022
SRR5849115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59219
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCGTCT6821.151657407251051No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGC6321.067225046015637No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCTTATAC1250.21108090308853575No Hit
AGCCTTGGGTGCTGAAAGAAATAGAGACATAAATAAATGGAATCACATCTCA1110.18743984194261978No Hit
GGAATGGGATGAAGTAACTCGCCAATTATTCTTAGGTTAACACACTCTTTGC1060.17899660581907834No Hit
TAAAGGGGGACGCGCCGGAAGTGCATTTGCAGAGTGAGACAAAGCGGAGAAC1050.17730795859437007No Hit
GCAACTGGCATCTTCATTTCAAATTAATTTTTGTGAGGACCCAGAGAAGTGG1030.17393066414495348No Hit
CTAGAGGGTACATTTTTCATAACGACCTGCAAATATAAAACCTTTCTTTCTC960.16211013357199547No Hit
GGATATGGGTGAACACATACGTTTCAGAAAAATTTGTACATTAAAGTGCATT920.15535554467316234No Hit
TTAACTGGGCAAGTGAAATATGAAAATGGTTTCCATAATAACAATTATTGAT920.15535554467316234No Hit
ACTGCCGGGCAAGCAATCCACATACTTAAAAAGCCATCAAAGCTAAGAATAG920.15535554467316234No Hit
TTGGATGGGAACCACATAAGTTGGGGAACATCTATTAGTCTGAAGCAATGGT910.15366689744845405No Hit
TCTAGTGGGATGAGCACCCCAGGCAGCTCAAGCTGAATAAATAGCTGCAGAC900.15197825022374575No Hit
AGTAATGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA890.15028960299903746No Hit
ACCTTCGGGGAAATCAGCAGGAGAGTAGAGGAGACACCAGCCCCACCAGGAG880.1486009557743292No Hit
GCTCCTGGGTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAG860.14522366132491263No Hit
AAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.14522366132491263No Hit
GTTTCTGGGACCTAAGGGAATTAGTTTTACACCTTCAGATAAAGACTGCTGA850.14353501410020433No Hit
CGGTTTGGGACACGTTTGAAACCAACTACTGTAGAAGATGAGGAATCTATTG840.14184636687549604No Hit
AGGTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA810.13678042520137118No Hit
CGACATGGGAGATACCAAAATCCATGAATTTTCAAATCCCCTTTATAAAATG810.13678042520137118No Hit
ATGTAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC810.13678042520137118No Hit
CTGTTTGGGGAACATCTGGGATGTGGGTGGCCAGAAGTCCCTGCGGTCCTAC810.13678042520137118No Hit
TACAGTGGGTATTTCTTCTAGTGTAATTTTTTTTAAAATTCATATTTGTGTC800.13509177797666289No Hit
TTGGTTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG800.13509177797666289No Hit
GAATACGGGGTGATTTTGTTCATTTATTATTTTCTATCTTCACATTGTAATG790.13340313075195462No Hit
TACAAAGGGTCTAACGTAGGGCCAGAAACAGATGATGAAGAAGAAAAAAAAC780.13171448352724632No Hit
GTGAGTGGGAATAGGATGTTAAAATTATCATCGTGTTTCCTATTTGTGTTTA780.13171448352724632No Hit
GAAGATGGGACCCACCACCAGCCGTCCCGCAAGCAGCATCTGTCTGTCCGTG780.13171448352724632No Hit
CATTCCGGGTGGTCCATGACTTGATTTTTTTCATTTAATTTGCAAACCAGCC780.13171448352724632No Hit
AACTATGGGCTCATGATCGATTACAAGTTTATGGAGGACACAAAGACATGAT760.12833718907782973No Hit
AAACTAGGGGCAGGGTTTAAAAATCAGTAAAAATGGGAATGATTGAGCTAAA750.12664854185312147No Hit
ACAAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA750.12664854185312147No Hit
TGTAAAGGGAAAATGAAAATGTTTCATTTTTAAAGGGATGGTAAGAATTTTT750.12664854185312147No Hit
ATTGGCGGGAAGAGGGAGGCTGGTTCTGTTGCTCGTATCAATAGGTTATCTG750.12664854185312147No Hit
AATAATGGGGGAGAAAGTGGCAACATTAATATAATCTTTTAATCATTTCTAA750.12664854185312147No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCT750.12664854185312147No Hit
GTGCTTGGGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAAC740.12495989462841317No Hit
CCAGCCTGGGTGACAGAGAGAGAGACCTTGTCTCAAAAAAACAAAACAAAAC740.12495989462841317No Hit
GCGCAGGGGCAAAAAGAAAATTTACAGAATAAGCCAATTCCTGGTAGAGAGA740.12495989462841317No Hit
GATGTGGGGCTGAAGGAAATCAAAGGAAATCTAAATAAGTGAAGAGATGTGA740.12495989462841317No Hit
GATCAAGGGAATGCAGAAGGAATATGGATATATTTCTTTAAGTCTGCAGATT740.12495989462841317No Hit
TAATAGGGGGGAACTCTAGAAGATAATGGTTAGGCAAGTGATTTTTTTTTTT740.12495989462841317No Hit
CTTATATGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC730.12327124740370489No Hit
AGCAATGGGTCTATACATTACAAGGAAATCACTTGGCTGCATTATGGTAAGT730.12327124740370489No Hit
TTAGCAGGGGATGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTG730.12327124740370489No Hit
GCAATGGGGGGAGACTAATGACTTTTTCCTTAATTTATTATCAAATTTGCCA730.12327124740370489No Hit
TGCGATGGGAGGGGCTGGCTAGCCGCCATCTTGCTTCTTTTTCTCGCTCGCT730.12327124740370489No Hit
GTAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA720.12158260017899661No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.12158260017899661No Hit
ATGAGTGGGCGAGACTAGCCTGGGCAACGTGGTAAAACCCTATCTCTATGAA720.12158260017899661No Hit
AGACGAGGGACGATAGAATGTTACTGATGATTCAGGGCTATTTCTGAAGTCT720.12158260017899661No Hit
CTAGGGGGAAAATAAAACTGAGGTGCAAAGAAACATCAGTGACAAATAGAGA720.12158260017899661No Hit
CACATAGGGTGCCTTTAGCTCTGTTTTATTAGACAGAGTTAAAGCATGTTGT710.11989395295428833No Hit
GTTGCCGGATGCTCATTATTACCACTTAGAAGATGTTGTTAAAAACATGTGA710.11989395295428833No Hit
TAAGTAGGGGAAGGATGCACATGAAGAAATGGAGCAGGCTGTGGAAGAATGT710.11989395295428833No Hit
GCTAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.11989395295428833No Hit
CTGCCAGGGAACTATATAGATAAGGAATACATTTTAAGCAAAGATTTTTCAT710.11989395295428833No Hit
GATTCAGGGCTGATCAAAGTACTGTTCCCTTCACTTATCGCTGCTTGTTACA710.11989395295428833No Hit
AGCCTGGGTGACAGAGGGAGATTCTGTCTCAACAAATAAACAATCCCCCCAA700.11820530572958002No Hit
AAGCATGGGCTCACTTATATCTTTTGCTTTCTTTAAATTCTGTAATGATTTC700.11820530572958002No Hit
TGCTTGGGGATTTCCATTTCTGGAAGTTGTAAGAGCTCACATTAAAGACAGT700.11820530572958002No Hit
GCACTGGGCTTTTAACTTTTCCAGCCTATTATAATAACTGAGTTATATTCAC700.11820530572958002No Hit
CAAAAAGGGCAGACAAACCTGTGGCATATTCTTTGTCAATGCCAACATTTTC700.11820530572958002No Hit
AGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.11820530572958002No Hit
AAGCCAGGGGGGCTCGAGACGCTGCGCGGCGGCAGCGGCGGGCGCGAGTGGC700.11820530572958002No Hit
CAGCCTGGTGAAAACATCTGAAAATAAGTCTACGGACTGTACTCAATCTACA700.11820530572958002No Hit
GTAGGAGGGAGCTTCAGAAAACAAGATGCCATAGTAAATTGAGAATAACATT700.11820530572958002No Hit
TACAGAGGGGGAAGAGAAGGAAGATTGCAAACACTCATAATCTATTATCTTT690.11651665850487174No Hit
ATCAGCGGGCAATGAAATGCAACTTTTAAATATTCCTCACTGTGTGTTTTAT690.11651665850487174No Hit
AATGTGGGGGGACAACCACACTGGGAGTGTGGGTCATAGAGTCTCAGGATCA690.11651665850487174No Hit
GAATTGGGGGGTGCAGTGGCATGATGTTAGTTACATAGCCTTTGAAGCCATG690.11651665850487174No Hit
GCAAGGTGGGGAAAAGCTCACAAAATTGCTAGCCACAAATACTATACCCAGC690.11651665850487174No Hit
CACTGCGGGGTATTTTAAAGGAGAGAAAGCAATCAACAACTCCTTTGTTTTT690.11651665850487174No Hit
CTTGCTGGGATAGCTACATGTACAGATGTTCACATTTAAGAGTAATGCGAGT690.11651665850487174No Hit
GGAACTGGGTCAACTAAAAATGTGACACACCTCAGGAATCAATAGTAGGAAT680.11482801128016346No Hit
TTATATGGGAGTGCTATAACTCTCCTGCCCATGATAGTGTATTCTGTTTCAG680.11482801128016346No Hit
GATTTTTAAACTTCATCCCTAGGATTGATATTGCTGATGATATTATTAATGC680.11482801128016346No Hit
TGCCCTGAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC680.11482801128016346No Hit
GAGAGGGGAAATCAAAGAACTGATGCACTATATAGAACGAATTTGGGTTTTT680.11482801128016346No Hit
TGAGGCAGGGTAAAAAGTTAAAGGTAATTAACAAGTATTATCTAGCCACATG670.11313936405545517No Hit
TGTGTCGGGATCTGAAGGACAAGGAATGCATCTAGAACAAGTCCCATGTTTT670.11313936405545517No Hit
TGATGTGGGACCCCTTGTTGAGGGGCTTCTATTACTCTTATCTGAATTCTAC670.11313936405545517No Hit
GCAAATGGGATCTTCAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT660.1114507168307469No Hit
CGCCTAGGGAGATTTTCTTAGATTTTAAATGAACCTTTTCAAATATGTCTGA660.1114507168307469No Hit
AATAAAGGGGGAGACTAAGATGTATTAACCTACCTGTGAATCATATGTTGTA660.1114507168307469No Hit
TAACAAGGGTTGAAGTTTAAACTCGGTTATCTCTTCATGAGACTATTATGTT660.1114507168307469No Hit
CGCTAAGGGGTATGTAAAAAATCTTACTTTAAGTAAACCTTACCATGAATTT660.1114507168307469No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCTGTC660.1114507168307469No Hit
CTGTTAATGGGATAGATGAGTTGCACACAAAGCCTAGTGTATTATGGAAGAC660.1114507168307469No Hit
TGCTGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA660.1114507168307469No Hit
ATCTGTGGGGGGGTCATTTATTTAGAGACACCTATACTTAGTAGGACATGTA660.1114507168307469No Hit
AACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT650.10976206960603861No Hit
ATGTGGGGAATTCTGGTCTAGAAGATAACCTTAGTACTTGGTAGAAATTTTG650.10976206960603861No Hit
CGTCTAGGGATGAAACTTGAGAAATCCTCTTGAGATAAAAGGCTGCCAAATC650.10976206960603861No Hit
TGTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC650.10976206960603861No Hit
ACACCTGGGAAGATGGTGGGGATGCCATACAACAGGACTATGTGATGATTTT650.10976206960603861No Hit
CTATCCGGGTATCTTGTTACATTTACTTTTTTAAATAAAGAAATTACAGAAG650.10976206960603861No Hit
CTACCTGGGGGAGTGTAGTACATATAAATGTGTGCTTATATGTGTATGTGTA650.10976206960603861No Hit
AATAGCGGGGACGCTCTTCCGATCTATAAATGGGGAAGAAAGCAAGGTGTTT650.10976206960603861No Hit
GATCTAGGGGTGGGCTGTGTGTACCTTGGTTGGTGACTCAGTGAGAAGAAGC640.10807342238133033No Hit
TTACATGGGAGAGTGAGTGGTTTTAGTGCATTACTCGAGAAGCCAATTAAAT640.10807342238133033No Hit
TAGCTTGGGATCTTCTTGTGGGGTTAAGGGGTAGAACCTATCTTGCCTTCAC640.10807342238133033No Hit
ACTCCTGGGGCTCCACGGTTACCCCGGCTCTCCGCCCCTCCTTCTCGCGGCG640.10807342238133033No Hit
TATAATGGGGCTCTCGCTATAGCTGTTGTCCAGATCGTTATCTTCTCAGAAA640.10807342238133033No Hit
TGTTTGGGGGAAAAAAGTCATACTAATTTGCTTCTTTTTCAATGTAGTGCTT640.10807342238133033No Hit
GTTTTGGGGCATATAGAGTTGAGAAAATGGATAAATTTTGCTTTATTTATTA640.10807342238133033No Hit
GCCTTAGGGGAGTGGCATTAGGGAGGGATTGTGAGAAATGACTTGTAAATAT640.10807342238133033No Hit
GCCACTGGGACCTGAGAAGGTAACAATTACTATTCAAATGAACCACAGGATA640.10807342238133033No Hit
GATACTGGGCGATCTGAGTTAGGGATTTTTAATATTACTGTCTTTATTCTTA630.10638477515662202No Hit
TATTTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC630.10638477515662202No Hit
ACATTGGGGGGGAGTGGCGTGCTGGGCGTGCGCGGCTGCGGTACGGCGTGTT630.10638477515662202No Hit
AATTAGGGCAGATGACTGAGCAGTTCTACAAAAAATGGCCAGCACGAGCGGG630.10638477515662202No Hit
ACAGCAGGGAATTTTACTCACCTTCTAAAACAGAACAATTGTATCAAATTTT630.10638477515662202No Hit
GCGCAGAGGCTACTGGGGGAACAAGATCGGCAAGCCCCACACTGTCCCTTGC630.10638477515662202No Hit
ATCGCGGGAATGCATAACGATAATAACTCAAAAGGTGGAAGAGTATAAAATT630.10638477515662202No Hit
AGTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC630.10638477515662202No Hit
CAACTAAGGGGGTTGGCATGCAATGAATACTTATTTACATGGGTGTTTTGAA620.10469612793191374No Hit
TTCATAGGGGTCACAGTTCTTCTTCAGATACTGATATTAATGAAATTCATAC620.10469612793191374No Hit
AAGTTAGGGCAAGAATTGAGGTTTGGGAAACTCCGCCTAGATATCAGAAGAT620.10469612793191374No Hit
TGTAGCGGGATGCCAGCAGCATTTTCCCTGCCCACACAGTTATGACAACCAA620.10469612793191374No Hit
GAATACGGGACTGACATAAGAACAATTGTAAAACTAGTAACATGTAGCAACC620.10469612793191374No Hit
TGTGATGGGAATGCATATGAGGTGCTCAAATAGTTGCTGAGTAAATAAATGA620.10469612793191374No Hit
ACACTGGGGATATGACTCAAGTGTGAGTGGTCAATCATCTTTTGTGACATCC620.10469612793191374No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.10469612793191374No Hit
GAGACAGGGGACCACTGCAACCACCAATAACAAAATGTATATTCTTTTTCAG620.10469612793191374No Hit
GTATCAGGGATGTTCAAATATGTTTAATAATGACCAAAGTGCATTCTGAGTT620.10469612793191374No Hit
AGCAGTGGGCTTCAGGAAAATGCCACTTCTGCTCATTGATGGGAAAAACCGA610.10300748070720546No Hit
TATCTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC610.10300748070720546No Hit
TGCATAGGGCGCGCCATTGCACTCCAGCCTGGGCAACAAGAGCAAAACTCTT610.10300748070720546No Hit
ACTCAGGAGGCTGAGGTGGGCGGATCACTGGAGCCCAGGAGGTTGAGGCTAC610.10300748070720546No Hit
TTATGGGACTTACTAAGAATCTCTTTAAATCAGAGTCAACCCATGGCATCTA610.10300748070720546No Hit
AACATCCGGGCACAAATTAAAGCAACTTTCTTTTTATTTAAAAATTGGTAAC610.10300748070720546No Hit
AGCCTGGGCGACAAGAGAGAAACTCCATCTCCAAAAAAAAGTGTCAGTTGAG610.10300748070720546No Hit
CAACTAGGGCAGTGTAGCAATTGGAAATATTTATAAGTTATATGAAAGCCTG600.10131883348249718No Hit
GCCCTCCCCGGCCTCCCAAGGTGCTGGGATTGCGAGCCTGAGCCACCATGAC600.10131883348249718No Hit
TAATTTAGGGTTATATACCATTATGTTACTCAAATTTAACAATATTAGAAAC600.10131883348249718No Hit
TTCAGGCGGGATCTTTGAATGAAAATAACACATTCAAACTGCTGTTTCTGTT600.10131883348249718No Hit
TACGATGGGAGAGGAAGAGAAGAAAGATGAGAAGAAAAAGGAAAAGAAAAAT600.10131883348249718No Hit
CGCAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.10131883348249718No Hit
CACCATGGGAAGCGTACATATAAAATAGTTGTATATTATGACATTTTAAATC600.10131883348249718No Hit
TTCAGAGGGAAGTTAAAAAGCTGCTGTACAGCAAAGGAGACAATCAACAAAG600.10131883348249718No Hit
AGTTGCGGGGGTTGCTGTTGTGCGAAGATCGTGTAACTTTAGCAGACATGTA600.10131883348249718No Hit
ACAATAGGGGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGA600.10131883348249718No Hit
TACGGACGGGGAAAAAAATGAATGCCATTTTCAAGTTCCCAAATTACTTCTA600.10131883348249718No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)