FastQCFastQC Report
Sat 31 Dec 2022
SRR5849114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58960
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCGTCT7361.248303934871099No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGC6031.0227272727272727No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCTTATAC1140.19335142469470826No Hit
AGTAATGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA1110.18826322930800543No Hit
GGAATGGGATGAAGTAACTCGCCAATTATTCTTAGGTTAACACACTCTTTGC990.16791044776119401No Hit
TGTGTCGGGATCTGAAGGACAAGGAATGCATCTAGAACAAGTCCCATGTTTT980.16621438263229307No Hit
AGCCTTGGGTGCTGAAAGAAATAGAGACATAAATAAATGGAATCACATCTCA970.16451831750339213No Hit
TTAACTGGGCAAGTGAAATATGAAAATGGTTTCCATAATAACAATTATTGAT940.15943012211668928No Hit
CACATAGGGTGCCTTTAGCTCTGTTTTATTAGACAGAGTTAAAGCATGTTGT930.15773405698778833No Hit
AATAATGGGGGAGAAAGTGGCAACATTAATATAATCTTTTAATCATTTCTAA920.15603799185888736No Hit
GAAACAGGGACTTGGATGAAGCTGCTAGAATGGTTGTAAAGCTCTCTGAAAT900.15264586160108548No Hit
GCTCCTGGGTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAG890.1509497964721845No Hit
GCAAGGTGGGGAAAAGCTCACAAAATTGCTAGCCACAAATACTATACCCAGC870.14755766621438263No Hit
TACAAAGGGTCTAACGTAGGGCCAGAAACAGATGATGAAGAAGAAAAAAAAC860.14586160108548168No Hit
TTAGCAGGGGATGGCAGAGATGGTTTTTTAATGAAATCCCACCATCTATCTG860.14586160108548168No Hit
CATTCCGGGTGGTCCATGACTTGATTTTTTTCATTTAATTTGCAAACCAGCC860.14586160108548168No Hit
ACTGCCGGGCAAGCAATCCACATACTTAAAAAGCCATCAAAGCTAAGAATAG850.14416553595658071No Hit
AAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.1424694708276798No Hit
GAAGATGGGACCCACCACCAGCCGTCCCGCAAGCAGCATCTGTCTGTCCGTG840.1424694708276798No Hit
TGTTTGGGGGAAAAAAGTCATACTAATTTGCTTCTTTTTCAATGTAGTGCTT840.1424694708276798No Hit
TAAAGGGGGACGCGCCGGAAGTGCATTTGCAGAGTGAGACAAAGCGGAGAAC830.14077340569877883No Hit
TCACATTGGGAACTGACTTTTATTCACTTGTTGTGACAAGTTTTTAATTACC810.13738127544097695No Hit
CCAGCCTGGGTGACAGAGAGAGAGACCTTGTCTCAAAAAAACAAAACAAAAC800.13568521031207598No Hit
GCGCAGGGGCAAAAAGAAAATTTACAGAATAAGCCAATTCCTGGTAGAGAGA800.13568521031207598No Hit
TTATCAGGGAGACAGTATTTCAAGAGAGTGGCAGGTCTGTTCCTGGTAAAAT780.13229308005427406No Hit
GAATACGGGGTGATTTTGTTCATTTATTATTTTCTATCTTCACATTGTAATG770.13059701492537315No Hit
TTATAAGGGATCAGCTTCAAGTCCATGGAGGACCTCGTAAATGAATTTGATG770.13059701492537315No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCT770.13059701492537315No Hit
GCCACTGGGACCTGAGAAGGTAACAATTACTATTCAAATGAACCACAGGATA770.13059701492537315No Hit
CTAGAGGGTACATTTTTCATAACGACCTGCAAATATAAAACCTTTCTTTCTC760.12890094979647218No Hit
GATACTGGGCGATCTGAGTTAGGGATTTTTAATATTACTGTCTTTATTCTTA750.12720488466757124No Hit
ATAGCAGGGATTTTAAAACTGAAACAATAACCAAATAACATTGGATAATAAA750.12720488466757124No Hit
GCAATGGGGGGAGACTAATGACTTTTTCCTTAATTTATTATCAAATTTGCCA750.12720488466757124No Hit
GATCAAGGGAATGCAGAAGGAATATGGATATATTTCTTTAAGTCTGCAGATT750.12720488466757124No Hit
GGATCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC750.12720488466757124No Hit
GAGAGGGGAAATCAAAGAACTGATGCACTATATAGAACGAATTTGGGTTTTT750.12720488466757124No Hit
GTGCTTGGGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAAC740.12550881953867027No Hit
ACAAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA740.12550881953867027No Hit
AGTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC740.12550881953867027No Hit
TCTCGAGGGAACTAAACTGGAAGGAGAAATATATAGGAAGGTGGGGACATAG740.12550881953867027No Hit
GTTTTAGGGATTTTGAATAAAATGAATCACAATGTCTCTTTTCATCAAAAAG730.12381275440976934No Hit
AACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT730.12381275440976934No Hit
AACATCCGGGCACAAATTAAAGCAACTTTCTTTTTATTTAAAAATTGGTAAC730.12381275440976934No Hit
ATAAGCGGGTATCTAAGAACTTGGAAGCCGTCAGCCAAGTCGCCACATTTCT720.12211668928086838No Hit
TTATATGGGAGTGCTATAACTCTCCTGCCCATGATAGTGTATTCTGTTTCAG720.12211668928086838No Hit
CGGTTTGGGACACGTTTGAAACCAACTACTGTAGAAGATGAGGAATCTATTG720.12211668928086838No Hit
CTGTTTGGGGAACATCTGGGATGTGGGTGGCCAGAAGTCCCTGCGGTCCTAC720.12211668928086838No Hit
TGTAAAGGGAAAATGAAAATGTTTCATTTTTAAAGGGATGGTAAGAATTTTT710.12042062415196743No Hit
TAACAAGGGTTGAAGTTTAAACTCGGTTATCTCTTCATGAGACTATTATGTT710.12042062415196743No Hit
ACGCCAGGGTGATATGTTGAATGTATTTGATTTTGAAAAGGCTGGGAATTCA710.12042062415196743No Hit
AGCCAAGAGAAAACTCAGCCAAACCAACAATTGGTTTTTAAAAAAAATCTAT710.12042062415196743No Hit
GGATATGGGTGAACACATACGTTTCAGAAAAATTTGTACATTAAAGTGCATT700.11872455902306649No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCA700.11872455902306649No Hit
AGGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.11872455902306649No Hit
TCTAGTGGGATGAGCACCCCAGGCAGCTCAAGCTGAATAAATAGCTGCAGAC690.11702849389416553No Hit
ACCTTCGGGGAAATCAGCAGGAGAGTAGAGGAGACACCAGCCCCACCAGGAG690.11702849389416553No Hit
GAGACAGGGGACCACTGCAACCACCAATAACAAAATGTATATTCTTTTTCAG690.11702849389416553No Hit
CGTCTAGGGATGAAACTTGAGAAATCCTCTTGAGATAAAAGGCTGCCAAATC680.11533242876526459No Hit
TTGGATGGGAACCACATAAGTTGGGGAACATCTATTAGTCTGAAGCAATGGT680.11533242876526459No Hit
CACCATGGGAAGCGTACATATAAAATAGTTGTATATTATGACATTTTAAATC680.11533242876526459No Hit
ACTAACGGGCAGTGAACTTGAAATTAGGCCTATAAAAAGTTATTCAAACACA680.11533242876526459No Hit
ATTTGTGGGAAGAACTTGTGGTCTTTTAAAACTGGGACTGATACTTTTTTGA670.11363636363636363No Hit
GTTTATGGGATAAGAAGCAAGTAGAAATGCCAGTTTCACATAAAATCTTTTA670.11363636363636363No Hit
ATGAGTGGGCGAGACTAGCCTGGGCAACGTGGTAAAACCCTATCTCTATGAA670.11363636363636363No Hit
AGTTGCGGGGGTTGCTGTTGTGCGAAGATCGTGTAACTTTAGCAGACATGTA670.11363636363636363No Hit
TAATTTAGGGTTATATACCATTATGTTACTCAAATTTAACAATATTAGAAAC660.11194029850746269No Hit
GTTGCCGGATGCTCATTATTACCACTTAGAAGATGTTGTTAAAAACATGTGA660.11194029850746269No Hit
ACACTGGGGATATGACTCAAGTGTGAGTGGTCAATCATCTTTTGTGACATCC660.11194029850746269No Hit
TAGCTCGGGTGGTACCAATTATATCATCTCAGTCTTGAATTTACTCACGCTG660.11194029850746269No Hit
GATTTTTAAACTTCATCCCTAGGATTGATATTGCTGATGATATTATTAATGC660.11194029850746269No Hit
ACTCAGGAGGCTGAGGTGGGCGGATCACTGGAGCCCAGGAGGTTGAGGCTAC660.11194029850746269No Hit
GAAGGTGGGTGCAGAGATAGAGATTGAAGTAGCCATTTGGAGATGAGAATTT660.11194029850746269No Hit
TATTGAGGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGAT660.11194029850746269No Hit
TAAGCAGGGGGAAAAAAATTTTAGCCTTATCTTAATCTGTCCCAACAGCAAT660.11194029850746269No Hit
CACTGCGGGGTATTTTAAAGGAGAGAAAGCAATCAACAACTCCTTTGTTTTT660.11194029850746269No Hit
ATGATCGGGATGGCAATTCACCTATTTTAGGAAAAATACTCTTTTCATAATA660.11194029850746269No Hit
ATCAGCGGGCAATGAAATGCAACTTTTAAATATTCCTCACTGTGTGTTTTAT650.11024423337856173No Hit
GTTTCTGGGACCTAAGGGAATTAGTTTTACACCTTCAGATAAAGACTGCTGA650.11024423337856173No Hit
AGCAATGGGAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTAC640.10854816824966079No Hit
AAGCATGGGCTCACTTATATCTTTTGCTTTCTTTAAATTCTGTAATGATTTC640.10854816824966079No Hit
TAGCTTGGGATCTTCTTGTGGGGTTAAGGGGTAGAACCTATCTTGCCTTCAC640.10854816824966079No Hit
ACTCCTGGGGCTCCACGGTTACCCCGGCTCTCCGCCCCTCCTTCTCGCGGCG640.10854816824966079No Hit
AACTATGGGCTCATGATCGATTACAAGTTTATGGAGGACACAAAGACATGAT640.10854816824966079No Hit
TTGGTTGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG640.10854816824966079No Hit
AGCAGTGGGCTTCAGGAAAATGCCACTTCTGCTCATTGATGGGAAAAACCGA630.10685210312075984No Hit
AGCCTGGGTGACAGAGGGAGATTCTGTCTCAACAAATAAACAATCCCCCCAA630.10685210312075984No Hit
CCTAAAGGGGAAGGGGAAAACTGCGCAGGGGTTAAGCCCCGTGGACCAAAGG630.10685210312075984No Hit
TATTTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC630.10685210312075984No Hit
CATGAAGGGATTCTGAGGGAACTGTAGGACCACTGAAGTTTTATTAAGAAAC630.10685210312075984No Hit
TGATAAGGGGGCCTTTCTCCGCTGGCAACGGCGCCGCTCCCCGCTCCTCCTC630.10685210312075984No Hit
GCTCACGGGCATTTTCCTAAGTGTTGGATAATTCCCTGACCTTTAAAAAGGA630.10685210312075984No Hit
TTAGTTGGGGAGGCTACAGTCAGAAAGGCTTAACATACTTTTGGAGTTCTGG630.10685210312075984No Hit
AGACGAGGGACGATAGAATGTTACTGATGATTCAGGGCTATTTCTGAAGTCT630.10685210312075984No Hit
ACAATAGGGGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGA630.10685210312075984No Hit
TTTTGAGGGGAAAATTGCAATCTGGATTCTTTGGACATGACATTTTTAACCA630.10685210312075984No Hit
CCGAGGGAGGGTTTGCCCAGAAGCAACACCAGAGAATTACAGACAACGCGCT620.10515603799185888No Hit
GATCTAGGGGTGGGCTGTGTGTACCTTGGTTGGTGACTCAGTGAGAAGAAGC620.10515603799185888No Hit
GCAAATGGGATCTTCAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT620.10515603799185888No Hit
TGTGATGGGAATGCATATGAGGTGCTCAAATAGTTGCTGAGTAAATAAATGA620.10515603799185888No Hit
TCTGCAGGGTAGGCCTTCTCCAACCATCTGCCTTATGAGGTTCACCCATTTC620.10515603799185888No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAAACTCGTATGC620.10515603799185888No Hit
TATTAAGGGATTCCAAAACATAATTGGAAAATAGAAACTGAGCCATTGCCAA620.10515603799185888No Hit
CTAGGGGGAAAATAAAACTGAGGTGCAAAGAAACATCAGTGACAAATAGAGA620.10515603799185888No Hit
AGCATAGGGGCATGAGGGAGCACTGGGTCTAATTTTTGGGGGCTGAGAAGGT620.10515603799185888No Hit
AGTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.10515603799185888No Hit
GTTACTGGGGAACTAAACGGGGTGAGGTCACATTCGGTTATCTCTAACGTTG620.10515603799185888No Hit
TATCACGGGGATATGTGTAGAATTTTTGTTTTATTTTTAAAATGTGTAAAAC620.10515603799185888No Hit
TGTCTGAGGGCATTCATTGCATGTTTATTATGCAAGTTTAAATGAACAAAGA610.10345997286295794No Hit
CGCCTAGGGAGATTTTCTTAGATTTTAAATGAACCTTTTCAAATATGTCTGA610.10345997286295794No Hit
TATCTGGGAGGTTTGCCATTAGGGAAGGAAAGAAGTAGAAAGCAACCTGAAA610.10345997286295794No Hit
TTGCACGGGGTGTTAATCAGATACAGAACTTCCAAGAACCAGATGGCCTTCA610.10345997286295794No Hit
TGCTTGGGGATTTCCATTTCTGGAAGTTGTAAGAGCTCACATTAAAGACAGT610.10345997286295794No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAA610.10345997286295794No Hit
GCAACTGGCATCTTCATTTCAAATTAATTTTTGTGAGGACCCAGAGAAGTGG610.10345997286295794No Hit
TGCCCTGAGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.10345997286295794No Hit
TTCAGAGGGAAGTTAAAAAGCTGCTGTACAGCAAAGGAGACAATCAACAAAG610.10345997286295794No Hit
GCTAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.10345997286295794No Hit
TCTATTGGGAAGGGCTACCTTTTGGATACAAGGTCCCAGAAATGGAGAAAAC610.10345997286295794No Hit
TGCTGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA610.10345997286295794No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG610.10345997286295794No Hit
CAGCCTGGTGAAAACATCTGAAAATAAGTCTACGGACTGTACTCAATCTACA610.10345997286295794No Hit
AGGTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA600.10176390773405698No Hit
TACAGAGGGGGAAGAGAAGGAAGATTGCAAACACTCATAATCTATTATCTTT600.10176390773405698No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.10176390773405698No Hit
TGATTAGGGCTTTTTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA600.10176390773405698No Hit
ACATTGGGGGGGAGTGGCGTGCTGGGCGTGCGCGGCTGCGGTACGGCGTGTT600.10176390773405698No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.10176390773405698No Hit
ACAGCAGGGAATTTTACTCACCTTCTAAAACAGAACAATTGTATCAAATTTT600.10176390773405698No Hit
AGCTGAGGGAAATTAGTCCTTCAAATACTGCTGTATTTTCAGAAAAAAAAAA600.10176390773405698No Hit
TAAGTAGGGGAAGGATGCACATGAAGAAATGGAGCAGGCTGTGGAAGAATGT600.10176390773405698No Hit
CCGAAAGGGACTCCAATATTCCGTTTTATAATAATTCAGAGCCCTGTGGCTT600.10176390773405698No Hit
TTTATAGGGAGAATTTGATTTTAGGAAGTTTGTCAAAAATGTAAAAAAAGAT600.10176390773405698No Hit
CGTTGGGGGAAGATATGTTTTTACATTTTATTTAATATTACATTGGAGATTC600.10176390773405698No Hit
AAGAGTGGGTAATCATATAGAAGGCATTAGTGAACACTTATTGAATTAACAA600.10176390773405698No Hit
GATCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA600.10176390773405698No Hit
TTTAAAGGGAGACAACAAGGATGAAAAACTTTGTCGTGATCCACTTCCACTT590.10006784260515604No Hit
AAGAAAGGGATCAAGAGGTTGGATAACTTATTTCTAAGCTCAAGGTTAAAAA590.10006784260515604No Hit
CTCATGGGATTGATTAATATGTCATTGAATCATACCTTCTGATTTTCTGTGA590.10006784260515604No Hit
CGCTAAGGGGTATGTAAAAAATCTTACTTTAAGTAAACCTTACCATGAATTT590.10006784260515604No Hit
AATTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC590.10006784260515604No Hit
TAACGGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG590.10006784260515604No Hit
TGCTCACGGGCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATT590.10006784260515604No Hit
GCCTTAGGGGAGTGGCATTAGGGAGGGATTGTGAGAAATGACTTGTAAATAT590.10006784260515604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)