FastQCFastQC Report
Sat 31 Dec 2022
SRR5849097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51435
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCGTCT7241.407601827549334No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGC6341.2326236998153008No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCTTATAC1220.2371925731505784No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCT930.18081073199183437No Hit
TCAGTTGGGAAGCTCACATGGCAGTGAGATGTTGAAAAGTCAAAAGAGACCT890.17303392631476622No Hit
CGTTATGGGACAAGATAAAAAAACAGTCCTATCAAAAAGTGGGCAAAGGATA810.15748031496062992No Hit
GCAGTGGGGGTCCATTTTTTTAAACAATAGAAATATGGTCAAGGTATTAATT770.14970350928356177No Hit
AAAGTAGGGGATGAAATGAAATGAAATGAAATGAAATAAATAAATAAATGAA750.1458151064450277No Hit
TTAGCAGGGATCTACAAAATGGTAGGCACTGTGATCATGATGAAAATGAATG750.1458151064450277No Hit
GTCTTTGGGGAAAAACCTAAACAGAAAGCTACTTCTAAAAGTAAAAAATCTG740.14387090502576066No Hit
ACGACGGGATTGTTCCTTGCAGTATAGAAGGCAAATGCAATTATAGTCTTTA720.13998250218722658No Hit
ATAAGGGGGGGCATGATGGGAGCAAAGAAGATGGACCTAGTCAGAGGTGAGG720.13998250218722658No Hit
GTTCAAGGGATGTATAATTAGAGTTTTCTAATTTCACTGTGAGATCTCTAAC710.13803830076795956No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC700.13609409934869252No Hit
GCTTTGGGACTAGTAAACCTGCTCTAAAAGAATTCAAGGGAAGCTTTTTAAA680.13220569651015845No Hit
CATGGAGGGACAAGAGAAGAACAAGGAGCTGATGGATGAATATAATCATTTA670.1302614950908914No Hit
TATTAGGGGAACTTGTACAAGTATTTTTACTGATGGAGCAGGAACTCACTGC660.1283172936716244No Hit
GTCGGTGGGAAGGATTAAACATCCAGAATACATACAAAACTCCCAAAATGCA650.12637309225235735No Hit
TGCTCAGGGAGAGAACGCATGAGTTGTTGCCCAGGTTTGGGGTTCCAGGAAG650.12637309225235735No Hit
CGATACGGGAAGCAGTAGAAGATCTATCTTCACAAAGTATGAGGGATGCCAG640.12442889083309029No Hit
CAATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA640.12442889083309029No Hit
TATGCCGGGATTTGGGAAGAATATTATTATACAGTAGTAATGTTTTGAATAT630.12248468941382328No Hit
CTCAGTGGGGTTTTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATATCT620.12054048799455623No Hit
CACCTCGGGTGCTTAGATCATCCATAGGTGTTTCTTCTGCTTGAAGCTGCTA620.12054048799455623No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCA620.12054048799455623No Hit
TAACCTGGGACTTCAAAAAGCCAAATCAAAGAATCATTGGTTTTCAAGAAGG620.12054048799455623No Hit
TAACAGGGGAACTTTACATTGGAAATTCAAAAAGTAATTTCAAAGACTTTTT610.1185962865752892No Hit
GCCAATGGGAAGAGTGTAATGATTTCACTTATCACATAGTATTTCACATAAT600.11665208515602217No Hit
TACGTTGGGACCCGGCCCTGGGAATATTTTCTATGTTACATTTATTTTTTTC600.11665208515602217No Hit
TTTGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC590.11470788373675514No Hit
AGCAAAGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA590.11470788373675514No Hit
TATGGCCGGGGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGGCACACC590.11470788373675514No Hit
GTTACCGGGGCGCGTGCGGCCTGGCGCTTCCGGCACCGGCCGAGGTGCGGGT590.11470788373675514No Hit
TTCTTAGGGACACGACATGCGGCTTTTCTGCATCAACTGCTATGACGGTTAA580.1127636823174881No Hit
GTGTGCGGGGAGGAATATGAAGTCCATTCCCTTCTTTCTTCTCTTGAATAAT580.1127636823174881No Hit
GTTTTTGGGTTTGCAGGATGACCTCAGGGCTATGTGGATTGAGTAATGGGAT580.1127636823174881No Hit
CTGTGTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT580.1127636823174881No Hit
AGCTATGGGGGAGACAGGTGGAAACCAAAGTTCGAGCTAAAATCCGTAAGAT580.1127636823174881No Hit
GCTTGCGGGGAAACAAGGACTGAGAGAGAAAGAAGGTCAGAAAAACACAGAG570.11081948089822105No Hit
GCGCCAGGGAAATGGGTCGCAGTCGCAGCCGCTCTCCACGGAGGGAACGTAG570.11081948089822105No Hit
CTCTACGGGGAAGAAAGAGAAGAAAAGAGGAAGAAGAAGGAGAAGAGACAAG570.11081948089822105No Hit
ACAAGCGGGAACCAAGAAAACTGATTCTTCCATCAATCTTGAGATGGTGGTC570.11081948089822105No Hit
ATGCACGGGGTTCCTTTAGCTGCGGGATTTCAGTGGTACCTTTCTCAAACTC570.11081948089822105No Hit
GACAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC560.10887527947895403No Hit
CTAGCAGGGATTCACTTAGAAATTTTATTTTCTTATAACTTAAGTGCAATAA560.10887527947895403No Hit
CAAAAAGGGACTCAGCCAACCAACAATGTTTGCCATACCCCAAATGCTGAAC550.10693107805968699No Hit
GTCAGAGGGAAATGAGCTCAAGTACATGAATGTTAGTTGTTATCACATACAG550.10693107805968699No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAA550.10693107805968699No Hit
AACTAAGGGCTGTTGAGGTCATAAAAATAATAAAAGTCCGAGAAACTGCCAC550.10693107805968699No Hit
CATGGGGGGAAGAGTTTCCGGGAAAAGGCAAGGCTTTGTTCCAGGTGAATCA550.10693107805968699No Hit
TTCTTGGGGCATCTCGCCGTGAGACAGCAAGTGTCGGATCCGCAGGCGCAGC550.10693107805968699No Hit
CTTGCACGGGAACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAG540.10498687664041995No Hit
TAGACCGGGATAACAATTAGAGAAGGCTTCTTATTGGTCTTACACAGAAAGA540.10498687664041995No Hit
TAAGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC540.10498687664041995No Hit
GTTCAAGGGTTATTATATTGTATGTTAATATTTTATGAACTGGTCAGCCATC540.10498687664041995No Hit
GACAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC540.10498687664041995No Hit
AAGTCAGGGGAGGGACCAGAGCGGCGTTCAGGAGCTCGTGAAGCAAGTGCTG540.10498687664041995No Hit
CTTCCAGGGGTGAGGCTGCACTTGATTATATTTGATTCTTTGTTCGTAATAC530.1030426752211529No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCTGTC530.1030426752211529No Hit
AAAAATGGGCGTCCGCCTCCCGGGTTCAAGCGATTCTCGTTACTCAGCCTCC530.1030426752211529No Hit
GGGACCGGGAAATACATAGGGAGCAAGTATGTTCAGATGTAGTTAAATGTTT520.10109847380188589No Hit
CTTCTGGGGACTGTTGTGATTTACATGTGGTTATAAAATCTAACATAGAAGT520.10109847380188589No Hit
ATCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA520.10109847380188589No Hit
CGCTATGGGATTTATGCGAGTTGCAACTATGCTGGCAACTGGAGGCAACAGA520.10109847380188589No Hit
CCTCGCGGGTAGAAAAAAATGTAGTGTGTTCCATTTAAAATTTTGGCATATG520.10109847380188589No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)