FastQCFastQC Report
Sat 31 Dec 2022
SRR5849096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51363
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCGTCT7161.3939995716761093No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGC6371.240192356365477No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCTTATAC1290.2511535541148297No Hit
CGTTATGGGACAAGATAAAAAAACAGTCCTATCAAAAAGTGGGCAAAGGATA880.17132955629538774No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCCTGTCTCT850.16548877596713588No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCA830.16159492241496798No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC770.14991336175846426No Hit
AAAGTAGGGGATGAAATGAAATGAAATGAAATGAAATAAATAAATAAATGAA760.1479664349823803No Hit
CAATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA740.1440725814302124No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAAACTCGTATGC730.14212565465412846No Hit
TTTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.13823180110196057No Hit
GCTTTGGGACTAGTAAACCTGCTCTAAAAGAATTCAAGGGAAGCTTTTTAAA670.13044409399762474No Hit
TCAGTTGGGAAGCTCACATGGCAGTGAGATGTTGAAAAGTCAAAAGAGACCT670.13044409399762474No Hit
AAGTCAGGGGAGGGACCAGAGCGGCGTTCAGGAGCTCGTGAAGCAAGTGCTG670.13044409399762474No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCTCCAATCTCGTATGCCTGTC670.13044409399762474No Hit
ACAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.12849716722154078No Hit
CGTTAGGGGATAGCATTTTAAAAAGCAAACAAAAATATTTGACCATCCAAAT640.1246033136693729No Hit
TAAGCAGGGAGACAGATCAACGAGACAGAAAGTCAACAAGGATACCCAGGAA630.12265638689328894No Hit
TACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA630.12265638689328894No Hit
GTTCAAGGGTTATTATATTGTATGTTAATATTTTATGAACTGGTCAGCCATC630.12265638689328894No Hit
GACTCAGGGAAGGCACACCTCATTTTGTTGCACTTTTATTGCACTTTTCAGA620.120709460117205No Hit
GCCAATGGGAAGAGTGTAATGATTTCACTTATCACATAGTATTTCACATAAT620.120709460117205No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCTCCAA620.120709460117205No Hit
CTTCCAGGGGTGAGGCTGCACTTGATTATATTTGATTCTTTGTTCGTAATAC610.11876253334112104No Hit
TTAGCAGGGATCTACAAAATGGTAGGCACTGTGATCATGATGAAAATGAATG610.11876253334112104No Hit
TTCTTAGGGACACGACATGCGGCTTTTCTGCATCAACTGCTATGACGGTTAA590.11486867978895314No Hit
TAGTGTGGGAGGGAAATCAAATTAATTTTTCTCGTTAGACATAAGGAAATTT590.11486867978895314No Hit
ACGACGGGATTGTTCCTTGCAGTATAGAAGGCAAATGCAATTATAGTCTTTA590.11486867978895314No Hit
GCAGTGGGGGTCCATTTTTTTAAACAATAGAAATATGGTCAAGGTATTAATT590.11486867978895314No Hit
AGCTATGGGGGAGACAGGTGGAAACCAAAGTTCGAGCTAAAATCCGTAAGAT590.11486867978895314No Hit
TTTCAAGGGGACTAGGAGCTATTTCTGACTGTAAATGAATTGTATAATAGCT590.11486867978895314No Hit
CACTAGGGGATCTAGGTACGGGAATAAAATTCTATTGCAAAAATTTCTTTTT580.11292175301286919No Hit
TAACAGGGGAACTTTACATTGGAAATTCAAAAAGTAATTTCAAAGACTTTTT580.11292175301286919No Hit
TTGGTGGGGCCTTATCAGAATCTAAATGAAAATAGCGAACATTTAAAAGCTA580.11292175301286919No Hit
TATGGCCGGGGCAGGGGGACCAGCAGGCATGGTTTGGTTTCGCTGGCACACC580.11292175301286919No Hit
GTTACCGGGGCGCGTGCGGCCTGGCGCTTCCGGCACCGGCCGAGGTGCGGGT580.11292175301286919No Hit
CTGGATGCGGGATTATGAAAATCTTTAAAAATACAGCAAAGTTGAATTTTAC570.11097482623678523No Hit
CATACGGGGATGAAAGCTATTAATATTCTAGAATAGATTAATAAATTGGCTA570.11097482623678523No Hit
ACACTGGGAGTGACACAGGAGAAAGCCAAAACATTATTAGATAAAGCCCTGA570.11097482623678523No Hit
CAAACCGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC570.11097482623678523No Hit
CGATACGGGAAGCAGTAGAAGATCTATCTTCACAAAGTATGAGGGATGCCAG560.1090278994607013No Hit
TCAAGCAGGGAGGATACAAAGTGTGATTTGTTGGAAACATGTCCCAAATTTC560.1090278994607013No Hit
GTTTTTGGGTTTGCAGGATGACCTCAGGGCTATGTGGATTGAGTAATGGGAT560.1090278994607013No Hit
TATTAGGGGAACTTGTACAAGTATTTTTACTGATGGAGCAGGAACTCACTGC560.1090278994607013No Hit
CAGCTAGGGATAACATGATCATCCGACGCTACTCAGATGAAAGTGGGAACAT550.10708097268461733No Hit
CTTGCTGGGACTATTGAGAACTTGATACCTACTAGGATGGTTAAAAGAAAAA550.10708097268461733No Hit
TATGCGGGGTGAACTGTCAAGCTGCCCAGTCAGATGGGCTGTTGCCATTTAA550.10708097268461733No Hit
GCCGATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA550.10708097268461733No Hit
ATCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA550.10708097268461733No Hit
CAAATTGGGCATAGTGAATGTAATGGTGGTCTCTCATTTTGTGTTTTCTAGT550.10708097268461733No Hit
TGAGACGGGGGAGCGCATTGATACTAGAAATGAGTTGGAAAGCTATGCCTAT550.10708097268461733No Hit
AGTATGGGGTCCATAGAGAGCAAATATTAAGTTAGTTGAGAAATGTAGATAA550.10708097268461733No Hit
GAACAGGGATTAACTACGTACCAACACAACACAAAGTATGCTACACTCATTC540.10513404590853338No Hit
TAGCGTGGGGACTAAAAATTTTAAAACTTACAGAAGTTTAAACTGTTGAAAA540.10513404590853338No Hit
TTCAGGGATGTGGTATAGATTTGCTTCCATATATTTATTTGTTTCTAAAGTC540.10513404590853338No Hit
CATGGAGGGACAAGAGAAGAACAAGGAGCTGATGGATGAATATAATCATTTA540.10513404590853338No Hit
ATAACCGGGGTCCATAAAGTTGTTAATTTATTATTTTATCTGTATTTTTATT540.10513404590853338No Hit
CATAGCGGGAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAA540.10513404590853338No Hit
AAAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.10318711913244942No Hit
GACAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC530.10318711913244942No Hit
ACACCTGGGCGCGCTGGGGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCT530.10318711913244942No Hit
GTCGGTGGGAAGGATTAAACATCCAGAATACATACAAAACTCCCAAAATGCA520.10124019235636549No Hit
ACGCCAGGAGGTCTCTTGTATAAGAAAAATAACCATTGTGGGGTTTTTGTGC520.10124019235636549No Hit
CTCAGTGGGGTTTTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATATCT520.10124019235636549No Hit
CTTGCACGGGAACTCAACAATGGGTTACATGGCAGCAAAGAAACACCTGGAG520.10124019235636549No Hit
CAAAAAGGGACTCAGCCAACCAACAATGTTTGCCATACCCCAAATGCTGAAC520.10124019235636549No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)