FastQCFastQC Report
Sat 31 Dec 2022
SRR5849092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19856
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC2621.3195004029008865No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT2271.1432312651087833No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC640.32232070910556004No Hit
GAAAATGGGAATAAAAATGAATTATATCAAGAGTTTATTTAGTTAATTGACA440.22159548751007255No Hit
CGCTTAGGGGTTAGTCACAAAATTATTTAGTCCAGTTTGCCAGCAAAAGTTA410.2064867042707494No Hit
AACGATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC410.2064867042707494No Hit
TGGTAGGGGGCATCTGATGTGTTCTTTGAACCAGAAGGTAAGCTTTTTTGGC390.19641418211120065No Hit
GGTACTGGGAAATATAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTA390.19641418211120065No Hit
CAGTAAGGGACTTTCAAGATTGAACATAATTACAGTAATGAATTATAAATGA390.19641418211120065No Hit
CGCAAGGGGATGTATAAGCAGTATGCTTTGATTTTTACTTAATTTAATCTTC390.19641418211120065No Hit
TAAGATGGGGAAGATTCAGCATACCAAAACTTACAGGATGTAGTAGCAAAAG370.1863416599516519No Hit
GTGTATGGGATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTATG370.1863416599516519No Hit
GTCAAGGGGAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCC350.17626913779210315No Hit
CACTGCGGGGTAATCTGAAATTTTGCCAGGCCTCATGTGTAGAATAAGTGGC350.17626913779210315No Hit
TTTTCAGGGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGTCAGGTG340.17123287671232876No Hit
AATCAAGGGATCGTCCTGTTGTGCTCAGCCCCAACATCCCAGACCCGTTACC340.17123287671232876No Hit
TGTAGGGGGAACAAAAAACAGCACAAGGAAAGCCCCCAAAATATTCCAGATT340.17123287671232876No Hit
GGAAATGGGATTGGGTGTTTTTGTTTTGTTTTTACTATGAAAAGACAGCTTA340.17123287671232876No Hit
CTGTGGGGGAGCCTGGGCTGGGTCCCCCAAAGGGACCATCCAGGTGATAACA330.1661966156325544No Hit
GGCTAAGGGAGGATTAATATAAAATTCATGGAAAATGATTAATGAAGCATGG330.1661966156325544No Hit
GGTGTAGGGACATGGAACCACAAAATACCAAAATAGCCAAAACTATCCTGAG320.16116035455278002No Hit
TTCAACGGGAATTCACTCAAAAATGTGACCGAGTAAGTATTTAGGAAGGAAT310.15612409347300563No Hit
AGCAGCGGAGTTACTGTAGATGAAATACAAGATGACAGCAGTGATTTGCATT310.15612409347300563No Hit
CAACCAGGGAAGGGTTCTCATTGTTGCCTTTTAAGAGGCCTAAGTCCTGCAT300.15108783239323126No Hit
GTAATGGGGCAGCTTTATTTTTCTTTAAAGCCAGTCATCATCTCTTGATAAA300.15108783239323126No Hit
TAGGCCGGGGAAGAAAACTTTGTTCATTTGTTTCTACTTTCTAAGAGAAATT300.15108783239323126No Hit
ATTTACGGGGGGCTTGCTAATATTGCCCTCAGAATTAACCTACCCACCCAGT300.15108783239323126No Hit
ACGTCGGGGAGTCATTCAGCTTACAGGTCTGCTTAAAAAACAGAACATTTTT300.15108783239323126No Hit
AAGCCCTGGGAACTCACTGAAATTTTTATATCCTTTTACCAGCATCTCTCCT300.15108783239323126No Hit
TCAAACGGGACTGGCTTGTGTTTTAGGGCACTCAATACATAAAGATGTGATT290.1460515713134569No Hit
GCACTGGCACCCTTAAAATACACAAACAGTAATCATTATTCTTGTTCACATC290.1460515713134569No Hit
GCTCTCCGGGCTCTGTCTCCCTATTCCTTCTGGTAAGAAGATAAAAGCTAAT290.1460515713134569No Hit
GCTGCTGGTGCTCACCCTCCTCTGCAGCCTGGTGCCCATCTGTGTGCTGCGC290.1460515713134569No Hit
AATCTGGGGATAAGAGGAACCGTTCAGAGTCTTCTCGCAGTTCCAGAAGGCA290.1460515713134569No Hit
TATTCGCGGGATCGCAGTTTAATCTACAAATGAAATTTCTACAGAAACAGGA290.1460515713134569No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA280.14101531023368252No Hit
GTGCTTGGGATTCTGCGGCTGTGGGCATGTGGCTCTCGCAGGCACCGTGGCG280.14101531023368252No Hit
GCACATGGGAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGAACAAGG280.14101531023368252No Hit
TCTAAAGGATCTGGTGAAGGAGTCAGATGAGTAAATAAATAAGCAAGACAAA280.14101531023368252No Hit
GTTATAGGGGTAGGATAGAAGGGACTCCAGTAGACAAAAGAATCAGATTCCT280.14101531023368252No Hit
TTTTCTGGGAACTTTTTTTGGTGAAGATTCATGGTTCCTAAGTTCTGTATTC280.14101531023368252No Hit
AGATATGGGGTGATGTGGTTAATGAAAAGAAGAGAACTCCAAATGAAACCAC280.14101531023368252No Hit
TATAACGGATTATTACTTCAGCATCAGGAAAGATTTCTTAAATAGGATATAA280.14101531023368252No Hit
GCTCTAGGGATCTTGTGTGGGGATCTGGAGCCATAGTGTCCCTGAGCGATGA280.14101531023368252No Hit
CAATGAGGGACCAAGCTCCTTCCTAAGTACTTTGAGGGTAAAATGAAGAAGG270.13597904915390813No Hit
CTAGTGGGGATATTGAAGTCGAGCAATTACTTTGCAATTTTGTACCTGACAA270.13597904915390813No Hit
AAATGTGGGACCATTCCCATTTTCTCAGCCTCTTCTCACTGCCTCCAGAGAG270.13597904915390813No Hit
GCTTACGGGCTGATTCATGAGCACATGGTTATTACTGATCGCATTGAAAACA270.13597904915390813No Hit
GCAAAAGGGCAGTCCACTAAAAACTAAGGGAAAGTATGTCTCCTGTAGTTAT270.13597904915390813No Hit
TTTAAAGGGACCACCTTAGATGGGTTTTTAATCCATACTTCAGCAAAATTCA260.13094278807413376No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT260.13094278807413376No Hit
AAACAGGGGCACACGGGCAAAGCTTGATTTTTATCATGTTGCAATTGTTAGC260.13094278807413376No Hit
TACTACGGGATATCAAAATCCAGTATTTGTAAAGCATTTAATAATATTTTTT260.13094278807413376No Hit
TTAATAGGGATTTTTGATTGTGAAAGCTCTGTTCATGTCATGATCACATGAT260.13094278807413376No Hit
GAAATCGGGGGAAGCAGGACTGGGGGTCTGGCTTGCCCCCTCCCTGGGCCTC260.13094278807413376No Hit
TTCACGGGGAACACATAAGGACTCGCATAAATTTAAAGTAAAGGGATGGAAA250.1259065269943594No Hit
GTCAATGGGATCTAATGAAGGGAGCTTTAACCAATTTGTCATAAATATGTTT250.1259065269943594No Hit
ATTCGAGGGAGATGTTTTTCTGTTTAATTTAGAAGACAGTATTTCTGAAAAA250.1259065269943594No Hit
TTACTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA250.1259065269943594No Hit
CTTAGTGGGTGTTTAATCTGTATGAAATGAAATTATAGCTGGGCACAGTGGT250.1259065269943594No Hit
CATATTGGGATTGGGCCGAATCCCTTCTGCTGTGGGCTATCAGCCTACCCTG250.1259065269943594No Hit
GCTTTGGGGACTCTGCTGCTAGGCACTCCTCTAAGCATCCTACAAATTTTAA250.1259065269943594No Hit
AGGTATGGGATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCTGAGG250.1259065269943594No Hit
GCTAGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC250.1259065269943594No Hit
AGTTGGGGACTATAATTGGATGAGAAAATTGTTAAACAATGTCACTTTCCAT250.1259065269943594No Hit
GCTTATGGGAGGAGACAATGGGAATGAGACTGGGCAGTTTTCTTCCTGTCAC250.1259065269943594No Hit
ACTACTGGGAAGAAAGAAGGTGCATTTCCAGTCACCCAGAACCGGGCGCTGC250.1259065269943594No Hit
CGAACGGGGATACTTGTCATCTATTAGTAGCTATCTAATCTACACACATACA250.1259065269943594No Hit
TGTTATGGGATACTGGGAATCTCTTCCTGAACAACGAATGTAAATTTGGTCA250.1259065269943594No Hit
CGCAAAGGGAGATAAATAGTCTGGCCTATGCAGAACAAAATGAAATGGTGAT240.12087026591458502No Hit
TAAAAGGGGAGTTCCTTCTTTCCCTTGGACGCCCAGTGCTGGCCTTGGAGGA240.12087026591458502No Hit
GACCTGGGGATCCTAGAAAATGTGCACCTTGATTCCTACCATTTCCATCACA240.12087026591458502No Hit
AATTCTGGGGGTCTCACTTCAGCCAGAGAGCGGGGTTAGGGCTTCATTGCCT240.12087026591458502No Hit
GTGCAAGGGATCTCTCTCAGGGAAAGAAACTGTTTAGATAATCTGCTTTTCA240.12087026591458502No Hit
GCCTCAGGAGGACACAGCTTTTGAGGTACCATCTTGGAAGCAGAGACCAGCC240.12087026591458502No Hit
GGCGCCGGGAGCTCCATCCATGTCCCTGCAAAGTACATGATCTCTTTCCTTT240.12087026591458502No Hit
GTATGGGACTGAAAAAGCACAAAATAAATCTCCTTCTAGACTTGATTCCTTT240.12087026591458502No Hit
GTTCTTGGGATAAACAGATTTACTGCATGACACATGATGAAGGATTTTATTG240.12087026591458502No Hit
CACCATGGGATGGTAAGCTCAATGGACTTTGAATTGTTTACAGTGCTGTATG230.11583400483481064No Hit
TGGACCTGGGATGTTTAAACTCTAGTTTAAAATTATCTCTTGTAGGTACAAA230.11583400483481064No Hit
GTTCATGGGACCAGTTTCTATGAGTGGCATGGTGTGAAAAAAGTTGAGGAGC230.11583400483481064No Hit
ATTTGAGGGATCGTTTCACTGGCACACAAAATTAATCCTTGTTAGACTGAAG230.11583400483481064No Hit
ACAACTGGGATTTTCAACTTTACTATATATTTCCAAAAAACTCTTCAAAGTG230.11583400483481064No Hit
TGCGCCGGCAAATGTGACTGAGTCATTACAGTGGACCCTCTGGGTGCATTGA230.11583400483481064No Hit
GTGATGGGGAGTGTAATATAAAGTGATAAATGTAGAATCGTGAAATTAGAAG230.11583400483481064No Hit
TAATTGGGGACTACATTAAAACCCATCTTTATTTTATTTTGTTGTTTTTGTT230.11583400483481064No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT230.11583400483481064No Hit
GAAACTGGGAGCCTACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCC230.11583400483481064No Hit
ATATCCGGGCTCTCAGCGGGAGTTTTACCACCTGAATACTGAAGCTGCCAAC230.11583400483481064No Hit
TTACAGGGGATGCCCTTACTTTAAAATAGTCCTTTTATTTTGTGTATTTTAT230.11583400483481064No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG230.11583400483481064No Hit
TTTCTGGGATAGCTAATAGTGAAATCTACAAACCAAAACAAGAACTTTTAAA230.11583400483481064No Hit
GTCAGGGTCTACACAGGGGGATAGGAAGGAGCTCAAAATTATGTGAGTTCAT220.11079774375503627No Hit
CGTACAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT220.11079774375503627No Hit
GCGACAGGGACTTCTGACTGGCTATAAATTGGAGTTCCCATGACCCCAGCCT220.11079774375503627No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC220.11079774375503627No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT220.11079774375503627No Hit
CTCTTAGGGGATATGAGATCTCTTTAACACCCCTCAGTCTATGTAGGAAATG220.11079774375503627No Hit
AGCTTGGGGACTGTGAGGATTAATTGAAATGGTTCATGAAAAGTTCCTAGTG220.11079774375503627No Hit
TAAAAAGGGCTTCTCGCCTAACGCTGCCAACATGGTGTTCAGGCGCTTCGTG220.11079774375503627No Hit
GACATTGGGGATCTTCTGGCGGGCGCTCTTCCGATCTGTTCGTGGGAGAGCG220.11079774375503627No Hit
TACCAGGGGGTCCTTGAATTTAACAGCAACCAACATATTGTTTGCATAGTTC220.11079774375503627No Hit
ACTTTAGGGATTGTGTAATAACTCACGTACTCTGAAGAGAGCTTGGTCAAAC220.11079774375503627No Hit
ATGGCTGGGAAGGATCTTATTCGAAGGCTCATTTGTAGCAGAGAACATCGAC220.11079774375503627No Hit
GGCTCTGGGAAAGTGGTCAGGGAAATGTAGTTCTTATTCTGCATACTGTTGT220.11079774375503627No Hit
GAGTGGGGGGCAAATCTGAGATTATAAAATGCCTTAGAGAATATACTAACTA220.11079774375503627No Hit
TCTATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC220.11079774375503627No Hit
TGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA220.11079774375503627No Hit
AACCTAGGGCAATGAAGCACCTAAAAACACTGCTTGATATTATAAATTTAAA220.11079774375503627No Hit
GTATTTGGGAAACATTAGTACTGAGGAAATAAAGAAAGGGAGAGAGGAAGAA220.11079774375503627No Hit
GCTCGCGGAGCGCCTGCTCTTCCTTGACTCAGCCCATCATCTGAAAATTCAG220.11079774375503627No Hit
GCTTAGGGATAGCAAGACCCTGTTTCTACAGAAAATTTAAAAATTTGCCAGG210.10576148267526189No Hit
AGGCAGCTGGGTTGCATGGAGAGGTCCAGGAGGGACCGGAGGTGTGACAGAT210.10576148267526189No Hit
GCTCATGGGATTGCAGCTATAAACCAGAGAAACAGTATCCATGTGCATAGTG210.10576148267526189No Hit
TGCGCTGGGACTGTAATTAGTTTTGCAAATAACCTCTTAATTTTCAACTAGA210.10576148267526189No Hit
GTAGAGGGGCTGTTGCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA210.10576148267526189No Hit
TATCTTGGGAAGCATACACAGGCACATGGCTTTAAGAACCACACTGATGCCT210.10576148267526189No Hit
CAACATGGGTCTTAAGTCGGGATTTTTAGAAGATTGTGTATTTCAAACAAAG210.10576148267526189No Hit
GACAACGGGATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGA210.10576148267526189No Hit
TTTGTGGGGGTCCTCCTTGTTTCTCTATTTTTGGTTATGAATGTTGGGGTTA210.10576148267526189No Hit
AACTGTGGGAGAATACACCTGGCATTTTATATGACAAAAGAATGGTCATTCT210.10576148267526189No Hit
AACCAAGGGAAGCACGTCACCTACAGTTTTGTGATGGTGCATTTTTGCATTG210.10576148267526189No Hit
TCGCTGGGGCGTACTCTGTATATGCACGCGCTGTGCATTCTGGGGCAGCCAC210.10576148267526189No Hit
AGTTGTGGGAAACAAGGTCTTGATAAGGATAATATCTGAAACTGATGCCATT210.10576148267526189No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA210.10576148267526189No Hit
TACAGGGGATGGATTCCAACCTTCCTTTTTTTAAATTTTCTCCAGTCCCTGG210.10576148267526189No Hit
TCACTAGGGAGAACTTGGATTTTTTTTTAACAAAAGTCCCAAAACACCAAAA210.10576148267526189No Hit
ACACATGGGGTTACACTCAGAACATAACCCCATCAACACTCCCAAATGCTAT210.10576148267526189No Hit
TAAGTCGGGGGTTCGAGATCAAGTTGTACAGGCTGTGCATTTTAAGATACTA210.10576148267526189No Hit
GCATTAGGGACAGACTGAATAAACAAGTGGTCTATTTAGGGCCACTTTTTTT200.1007252215954875No Hit
TGTAGAGGGATTCTATAACTCCCAACATTTTGCTTCTTGACTATTGATTTAA200.1007252215954875No Hit
TCTACGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT200.1007252215954875No Hit
GCTTATGGGATCCTGATCAAAGTCCAGACAGGTTTTGGGGGGGGCTCAGGTC200.1007252215954875No Hit
GTGCCAGGACTGGTCGTCTTGTTATAGGAGATCACAAATCAACATCTCACTT200.1007252215954875No Hit
GAACGGGCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAA200.1007252215954875No Hit
TTTTCGGGATTTGGCTTTGTAAGCATAAGCTACGTAAGACATACCACACCTA200.1007252215954875No Hit
CTCGCAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC200.1007252215954875No Hit
ATGGCGGGCTGCCCTGCTGGCTTCCTGACTGGAGAGACAGGACCCACAGAAA200.1007252215954875No Hit
TTCGCTGGGAGTCATTGTGTTTGCCAGTAATGTTTGAGAGACATGTAAGTTG200.1007252215954875No Hit
AAACACGGGACTTAAAATGAGTTTTTAAAAGTGAAAAAGAAATGACTATATA200.1007252215954875No Hit
GCTACTGGGATCATCTATCTTTCTATCACTCTATCATCTATCTATTATCTAT200.1007252215954875No Hit
TGGCATGGGGCATTCTCCGTTACAAAGACTTTTTGTAGCCCTGATGATGAAA200.1007252215954875No Hit
TTGAAGGGGATTGAATTTTGAATTTGAAGTTAAAATTATTATTTGTATTTTA200.1007252215954875No Hit
TACTAGGGGACTCTTTTTATAATTTTTTCTAAAAAAAATTAGTGTTTGTTCC200.1007252215954875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)