FastQCFastQC Report
Sat 31 Dec 2022
SRR5849091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19658
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC2241.1394851968664157No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT2161.0987892969783295No Hit
GGTGTAGGGACATGGAACCACAAAATACCAAAATAGCCAAAACTATCCTGAG430.21874046189846374No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC420.21365347441245297No Hit
GAAATCGGGGGAAGCAGGACTGGGGGTCTGGCTTGCCCCCTCCCTGGGCCTC420.21365347441245297No Hit
CAGTAAGGGACTTTCAAGATTGAACATAATTACAGTAATGAATTATAAATGA390.1983925119544206No Hit
TTACAGGGGATGCCCTTACTTTAAAATAGTCCTTTTATTTTGTGTATTTTAT370.18821853698239902No Hit
TGGTAGGGGGCATCTGATGTGTTCTTTGAACCAGAAGGTAAGCTTTTTTGGC350.17804456201037747No Hit
TCTAAAGGATCTGGTGAAGGAGTCAGATGAGTAAATAAATAAGCAAGACAAA350.17804456201037747No Hit
TAAGATGGGGAAGATTCAGCATACCAAAACTTACAGGATGTAGTAGCAAAAG340.17295757452436666No Hit
GTAATGGGGCAGCTTTATTTTTCTTTAAAGCCAGTCATCATCTCTTGATAAA340.17295757452436666No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC340.17295757452436666No Hit
CTAGTGGGGATATTGAAGTCGAGCAATTACTTTGCAATTTTGTACCTGACAA340.17295757452436666No Hit
AGCTTGGGGACTGTGAGGATTAATTGAAATGGTTCATGAAAAGTTCCTAGTG340.17295757452436666No Hit
AACGATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC340.17295757452436666No Hit
ACTGCAGGGATGAATGTAGATCTCAGGTTAGGTTTAAGCTGACTGTCAAGTT330.1678705870383559No Hit
GTTCATGGGACCAGTTTCTATGAGTGGCATGGTGTGAAAAAAGTTGAGGAGC330.1678705870383559No Hit
ACGTCGGGGAGTCATTCAGCTTACAGGTCTGCTTAAAAAACAGAACATTTTT330.1678705870383559No Hit
TGTAGGGGGAACAAAAAACAGCACAAGGAAAGCCCCCAAAATATTCCAGATT330.1678705870383559No Hit
AGCAGCGGAGTTACTGTAGATGAAATACAAGATGACAGCAGTGATTTGCATT330.1678705870383559No Hit
ATGACTGGGAGGAAAAATATTTAATGAAATGCAAAGACTTATAAATGAAGAT320.1627835995523451No Hit
CACTGCGGGGTAATCTGAAATTTTGCCAGGCCTCATGTGTAGAATAAGTGGC320.1627835995523451No Hit
GTGATGGGGAGTGTAATATAAAGTGATAAATGTAGAATCGTGAAATTAGAAG320.1627835995523451No Hit
TATTCGCGGGATCGCAGTTTAATCTACAAATGAAATTTCTACAGAAACAGGA320.1627835995523451No Hit
AGATACGGGGTGGCTGTTGAGCACTAACTAGTACAGTCCAGTGCCATGGCCT310.1576966120663343No Hit
GCTCTCCGGGCTCTGTCTCCCTATTCCTTCTGGTAAGAAGATAAAAGCTAAT310.1576966120663343No Hit
GCTAGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC310.1576966120663343No Hit
TTTTCTGGGAACTTTTTTTGGTGAAGATTCATGGTTCCTAAGTTCTGTATTC310.1576966120663343No Hit
TCTATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC310.1576966120663343No Hit
GGCGCCGGGAGCTCCATCCATGTCCCTGCAAAGTACATGATCTCTTTCCTTT310.1576966120663343No Hit
TCTACGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT300.15260962458032354No Hit
AGATATGGGGTGATGTGGTTAATGAAAAGAAGAGAACTCCAAATGAAACCAC300.15260962458032354No Hit
GTGTATGGGATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTATG300.15260962458032354No Hit
AACCTAGGGCAATGAAGCACCTAAAAACACTGCTTGATATTATAAATTTAAA300.15260962458032354No Hit
GACCTGGGGATCCTAGAAAATGTGCACCTTGATTCCTACCATTTCCATCACA290.14752263709431274No Hit
ATTTGAGGGATCGTTTCACTGGCACACAAAATTAATCCTTGTTAGACTGAAG290.14752263709431274No Hit
GACAACGGGATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGA290.14752263709431274No Hit
ATTACAGGGATTGAAATGTTTTGAAACTGAAAAAAAATTTTACAGCTACTGA290.14752263709431274No Hit
TAAGTCGGGGGTTCGAGATCAAGTTGTACAGGCTGTGCATTTTAAGATACTA290.14752263709431274No Hit
GTCAAGGGGAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCC280.14243564960830196No Hit
TTTAAAGGGACCACCTTAGATGGGTTTTTAATCCATACTTCAGCAAAATTCA280.14243564960830196No Hit
GAAATAGGGATACCCGGCTAATTTTGTATTTTTAGTAGACATGGGTTTTCTC280.14243564960830196No Hit
GGCTAAGGGAGGATTAATATAAAATTCATGGAAAATGATTAATGAAGCATGG280.14243564960830196No Hit
GGAAATGGGATTGGGTGTTTTTGTTTTGTTTTTACTATGAAAAGACAGCTTA280.14243564960830196No Hit
ATATATGGGACACATAGAAGGTGGCCACATAGGTCTCTGGGGTGAGAAGACA280.14243564960830196No Hit
GTCAGGGTCTACACAGGGGGATAGGAAGGAGCTCAAAATTATGTGAGTTCAT270.1373486621222912No Hit
GCAATTGGGGAGCGAGAGTGATGATGGCTTCAGCATACACAATGCTACACTG270.1373486621222912No Hit
CTAAATGGGATGTATGGCCCAATGTATTTGAGACATCTCCTCATGTAATTCA270.1373486621222912No Hit
CGCAAGGGGATGTATAAGCAGTATGCTTTGATTTTTACTTAATTTAATCTTC270.1373486621222912No Hit
TTCAACGGGAATTCACTCAAAAATGTGACCGAGTAAGTATTTAGGAAGGAAT270.1373486621222912No Hit
AAACACGGGACTTAAAATGAGTTTTTAAAAGTGAAAAAGAAATGACTATATA270.1373486621222912No Hit
TTCCTTGGGAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACAC270.1373486621222912No Hit
GCTGTTGGGAGATGAAAAGGGAGTAAAGGAAAAAAGAGGTCAACCACTGTTT260.1322616746362804No Hit
GCCATAGGGGTTACAGCCCTAGAATAGAATACTAATTGGTATGTATAAAGAA260.1322616746362804No Hit
TGTAGAGGGATTCTATAACTCCCAACATTTTGCTTCTTGACTATTGATTTAA260.1322616746362804No Hit
AATCCTGGGCTCTCTGCAACTTCCGCCTCCCAGGTTCAAGTGATCCTCCTGC260.1322616746362804No Hit
CGCTTAGGGGTTAGTCACAAAATTATTTAGTCCAGTTTGCCAGCAAAAGTTA260.1322616746362804No Hit
GGTACTGGGAAATATAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTA260.1322616746362804No Hit
TCACATGGGGGGCTTACATCCCCCAGGTACATACTTTTACTTATTGTGGGAT260.1322616746362804No Hit
AGTTGGGGACTATAATTGGATGAGAAAATTGTTAAACAATGTCACTTTCCAT260.1322616746362804No Hit
TGCGCCGGCAAATGTGACTGAGTCATTACAGTGGACCCTCTGGGTGCATTGA260.1322616746362804No Hit
GCTTATGGGAGGAGACAATGGGAATGAGACTGGGCAGTTTTCTTCCTGTCAC260.1322616746362804No Hit
GTAGCTGGGGGTACAGGAGAGAATTTGTGATGATGAGCTGATCTTAATCAAA260.1322616746362804No Hit
GAAAATGGGAATAAAAATGAATTATATCAAGAGTTTATTTAGTTAATTGACA260.1322616746362804No Hit
TTCACGGGGAACACATAAGGACTCGCATAAATTTAAAGTAAAGGGATGGAAA250.1271746871502696No Hit
CTGTGGGGGAGCCTGGGCTGGGTCCCCCAAAGGGACCATCCAGGTGATAACA250.1271746871502696No Hit
GCGACAGGGACTTCTGACTGGCTATAAATTGGAGTTCCCATGACCCCAGCCT250.1271746871502696No Hit
GAACGGGCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAA250.1271746871502696No Hit
GAGTGGGGGGCAAATCTGAGATTATAAAATGCCTTAGAGAATATACTAACTA250.1271746871502696No Hit
GCTACTGGGATCATCTATCTTTCTATCACTCTATCATCTATCTATTATCTAT250.1271746871502696No Hit
GAAACTGGGAGCCTACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCC250.1271746871502696No Hit
GCACAGGGGGGCAAATTATAGCAACAGACAGTGAGGAAGTAAGGGAAGAAAT250.1271746871502696No Hit
ATATCCGGGCTCTCAGCGGGAGTTTTACCACCTGAATACTGAAGCTGCCAAC250.1271746871502696No Hit
CGTACAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT240.12208769966425882No Hit
ACAAGAGGGATTGTTACTATTATTTATTAAAATTATTATGAGTAAAATGTTA240.12208769966425882No Hit
GTGCTTGGGATTCTGCGGCTGTGGGCATGTGGCTCTCGCAGGCACCGTGGCG240.12208769966425882No Hit
CTTAGTGGGTGTTTAATCTGTATGAAATGAAATTATAGCTGGGCACAGTGGT240.12208769966425882No Hit
GTGCCAGGACTGGTCGTCTTGTTATAGGAGATCACAAATCAACATCTCACTT240.12208769966425882No Hit
TGTACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA240.12208769966425882No Hit
TTTGCAGGTCAGGAGCTGAAGTCAGGATCAGAAACCACCAACTGTACTGCAA240.12208769966425882No Hit
ATTTACGGGGGGCTTGCTAATATTGCCCTCAGAATTAACCTACCCACCCAGT240.12208769966425882No Hit
ACTACTGGGAAGAAAGAAGGTGCATTTCCAGTCACCCAGAACCGGGCGCTGC240.12208769966425882No Hit
TAATTGGGGACTACATTAAAACCCATCTTTATTTTATTTTGTTGTTTTTGTT240.12208769966425882No Hit
ATAACTGGGAATGAAGAAATGGAGGTCTAGAGATGTTAAATATCTTAGCCTG240.12208769966425882No Hit
GTTTGAGGGGTGGGCCTAATTCCTAGAAGGCAAGTCCCTGGATAGGAAAGGT240.12208769966425882No Hit
ACTTTTGGGGACAATATTTATAATGTTGATCTGGCTTATTTCAGTTAGCATG240.12208769966425882No Hit
CGAACGGGGATACTTGTCATCTATTAGTAGCTATCTAATCTACACACATACA240.12208769966425882No Hit
GGCGATGGGATCGGCCACTTATTCGTGATGCCAACAATTTTGTATTAGATGA240.12208769966425882No Hit
GTTTAAGGGGACGGCAGATGCAGCGAAGAGGCTCAATGTTACACCACTGGCA230.11700071217824803No Hit
AGGCAGCTGGGTTGCATGGAGAGGTCCAGGAGGGACCGGAGGTGTGACAGAT230.11700071217824803No Hit
GTATGTGGGCCTGGACATCACTCTACTCCTGACGTCCCTGCTCAAGAATCAA230.11700071217824803No Hit
AATTCTGGGGGTCTCACTTCAGCCAGAGAGCGGGGTTAGGGCTTCATTGCCT230.11700071217824803No Hit
AACCAAGGGAAGCACGTCACCTACAGTTTTGTGATGGTGCATTTTTGCATTG230.11700071217824803No Hit
AGTATGGGAACCAAAACTAATGAGAGGCTAGCAGAGGTCAACACCAAACTTC230.11700071217824803No Hit
GTATGGGACTGAAAAAGCACAAAATAAATCTCCTTCTAGACTTGATTCCTTT230.11700071217824803No Hit
CACAAGGGGTGAAGGGGGAATGATCTGAGCCTTGGTTCCCCTGACACGTCTT230.11700071217824803No Hit
ATAATCGGGGAGTTCTGAGCCTCTCAAGTTCCTTTCCCCAGTTAGAGTGGGG230.11700071217824803No Hit
GTATGAGGGATTTTTGGGAGTGTCTATCCTTCAATGGCTGAATAGTTCCTTA230.11700071217824803No Hit
GAACAGGGAGATATACCCAGTGAGACTTGTGTTCATGTGTTAACGATGCCAT230.11700071217824803No Hit
GCTCGCGGAGCGCCTGCTCTTCCTTGACTCAGCCCATCATCTGAAAATTCAG230.11700071217824803No Hit
ATCGGCGGGTATGTATTCTGTTAACGTAAAGAGTAACTCTTTTTGATACTAT220.11191372469223726No Hit
GTAGATGGGCAGCCCAAGTACCCTCCTCCAGAAGTCTGTGACTACCTTGTCA220.11191372469223726No Hit
TGCACAGGGATCATTTAAAAAGATTTACTGCAAACTTGAAAAAGGGATTTCT220.11191372469223726No Hit
CACCATGGGATGGTAAGCTCAATGGACTTTGAATTGTTTACAGTGCTGTATG220.11191372469223726No Hit
GCACATGGGAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGAACAAGG220.11191372469223726No Hit
TGCTAGGGGACTTGCCTGTTCTGTGGAGAAACTTTTCTTTTTGAGGGCTGTG220.11191372469223726No Hit
AGGCGGGGGACCCATAGCAGCACAAGATGCAAGTCTTACCCCCAAATGCTTA220.11191372469223726No Hit
GTTATAGGGGTAGGATAGAAGGGACTCCAGTAGACAAAAGAATCAGATTCCT220.11191372469223726No Hit
AAATGTGGGACCATTCCCATTTTCTCAGCCTCTTCTCACTGCCTCCAGAGAG220.11191372469223726No Hit
TATCTTGGGAAGCATACACAGGCACATGGCTTTAAGAACCACACTGATGCCT220.11191372469223726No Hit
CAACATGGGTCTTAAGTCGGGATTTTTAGAAGATTGTGTATTTCAAACAAAG220.11191372469223726No Hit
GCTTACGGGCTGATTCATGAGCACATGGTTATTACTGATCGCATTGAAAACA220.11191372469223726No Hit
AACTGTGGGAGAATACACCTGGCATTTTATATGACAAAAGAATGGTCATTCT220.11191372469223726No Hit
AATCAAGGGATCGTCCTGTTGTGCTCAGCCCCAACATCCCAGACCCGTTACC220.11191372469223726No Hit
AAGCCCTGGGAACTCACTGAAATTTTTATATCCTTTTACCAGCATCTCTCCT220.11191372469223726No Hit
ACACATGGGGTTACACTCAGAACATAACCCCATCAACACTCCCAAATGCTAT220.11191372469223726No Hit
TCTAGCGGGCTCGCACAGGGACACACAAAGAATTAAGTAGAGTGGCTTCAAA220.11191372469223726No Hit
AGGACCGGGACGGAGTTTGAACTCCATTCACCATCACCTTTGAGAATTTGGC210.10682673720622649No Hit
GCTTAGGGGCTATCCTCTGGGTCCTCCTTTTCACTCCCTGCTCCTTTGTCTG210.10682673720622649No Hit
CGCAAAGGGAGATAAATAGTCTGGCCTATGCAGAACAAAATGAAATGGTGAT210.10682673720622649No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT210.10682673720622649No Hit
TAAAAGGGGAGTTCCTTCTTTCCCTTGGACGCCCAGTGCTGGCCTTGGAGGA210.10682673720622649No Hit
CATATTGGGATTGGGCCGAATCCCTTCTGCTGTGGGCTATCAGCCTACCCTG210.10682673720622649No Hit
TTTTCAGGGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGTCAGGTG210.10682673720622649No Hit
GTCAGGGGGATTTGGGAACAAAGAGAGTTTTCATCTTTTTTTCAGATCAAAA210.10682673720622649No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC210.10682673720622649No Hit
AATCTGGGGATAAGAGGAACCGTTCAGAGTCTTCTCGCAGTTCCAGAAGGCA210.10682673720622649No Hit
CAGCCTGGGGGCCTATGGGCTGAGCGCGGGGAGTTACGCCCCGCTGGTTTTC200.10173974972021568No Hit
GCTCCAGGGTAATGGTGCAATTTTGCACGTGTGTGTGGACGCACTGGCCTAG200.10173974972021568No Hit
GTTTGCGGGGGAGAGAAGGCAGCAAGAATTATGACTAATTCAAAACTGGGTT200.10173974972021568No Hit
GCTTAGGGATAGCAAGACCCTGTTTCTACAGAAAATTTAAAAATTTGCCAGG200.10173974972021568No Hit
TCAAACGGGACTGGCTTGTGTTTTAGGGCACTCAATACATAAAGATGTGATT200.10173974972021568No Hit
CAATGAGGGACCAAGCTCCTTCCTAAGTACTTTGAGGGTAAAATGAAGAAGG200.10173974972021568No Hit
GAAAGTGGGATCATGCAGCATCTGGAACAGAGAAGACATTTTCATTTATGTT200.10173974972021568No Hit
CAGTAGGGTGAAGAACTAAATATAAAAGTGCAATTGCAAAATACTAATTCAC200.10173974972021568No Hit
CTTCATTGGGGCTATCTAGAATACAGGTCTTCCAGTCAGCTGGTCATTTACC200.10173974972021568No Hit
CTCGAGGGGAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATG200.10173974972021568No Hit
TACTACGGGATATCAAAATCCAGTATTTGTAAAGCATTTAATAATATTTTTT200.10173974972021568No Hit
TACCAGGGGGTCCTTGAATTTAACAGCAACCAACATATTGTTTGCATAGTTC200.10173974972021568No Hit
TCGCTGGGGCGTACTCTGTATATGCACGCGCTGTGCATTCTGGGGCAGCCAC200.10173974972021568No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA200.10173974972021568No Hit
TCACTAGGGAGAACTTGGATTTTTTTTTAACAAAAGTCCCAAAACACCAAAA200.10173974972021568No Hit
TAGCATGGGAGTAAACTGAAACATAAAGTAAACATGCATACAAATAAAAAAC200.10173974972021568No Hit
TTAAAAGGGATAGTATTCTGACTTGACAACTTAGCTAAGTTTATTAGGAATG200.10173974972021568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)