FastQCFastQC Report
Sat 31 Dec 2022
SRR5849090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19833
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC2321.1697675591186407No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT2041.0285887157767357No Hit
GGTGTAGGGACATGGAACCACAAAATACCAAAATAGCCAAAACTATCCTGAG540.2722734835879595No Hit
TTTAAAGGGACCACCTTAGATGGGTTTTTAATCCATACTTCAGCAAAATTCA400.201684061917007No Hit
CAGTAAGGGACTTTCAAGATTGAACATAATTACAGTAATGAATTATAAATGA400.201684061917007No Hit
GAAAATGGGAATAAAAATGAATTATATCAAGAGTTTATTTAGTTAATTGACA400.201684061917007No Hit
GTGCTTGGGATTCTGCGGCTGTGGGCATGTGGCTCTCGCAGGCACCGTGGCG390.19664196036908183No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC390.19664196036908183No Hit
CGCTTAGGGGTTAGTCACAAAATTATTTAGTCCAGTTTGCCAGCAAAAGTTA370.18655775727323148No Hit
CACTGCGGGGTAATCTGAAATTTTGCCAGGCCTCATGTGTAGAATAAGTGGC370.18655775727323148No Hit
TTTGCGGGGGCCCTATACCACCTGTACAAGACAAATTCCTTTTCAACTTCTT360.18151565572530629No Hit
TCTATTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC360.18151565572530629No Hit
AAGCCCTGGGAACTCACTGAAATTTTTATATCCTTTTACCAGCATCTCTCCT360.18151565572530629No Hit
TATTCGCGGGATCGCAGTTTAATCTACAAATGAAATTTCTACAGAAACAGGA360.18151565572530629No Hit
TTCACGGGGAACACATAAGGACTCGCATAAATTTAAAGTAAAGGGATGGAAA350.17647355417738111No Hit
TGGTAGGGGGCATCTGATGTGTTCTTTGAACCAGAAGGTAAGCTTTTTTGGC350.17647355417738111No Hit
GTTATAGGGGTAGGATAGAAGGGACTCCAGTAGACAAAAGAATCAGATTCCT340.17143145262945594No Hit
GCCATAGGGGTTACAGCCCTAGAATAGAATACTAATTGGTATGTATAAAGAA330.16638935108153077No Hit
AGGCAGCTGGGTTGCATGGAGAGGTCCAGGAGGGACCGGAGGTGTGACAGAT330.16638935108153077No Hit
GGCGCCGGGAGCTCCATCCATGTCCCTGCAAAGTACATGATCTCTTTCCTTT330.16638935108153077No Hit
AAATGTGGGACCATTCCCATTTTCTCAGCCTCTTCTCACTGCCTCCAGAGAG320.1613472495336056No Hit
TATCTTGGGAAGCATACACAGGCACATGGCTTTAAGAACCACACTGATGCCT320.1613472495336056No Hit
AACGATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC320.1613472495336056No Hit
GTCAAGGGGAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCC310.15630514798568043No Hit
TAAGATGGGGAAGATTCAGCATACCAAAACTTACAGGATGTAGTAGCAAAAG310.15630514798568043No Hit
GTTTAAGGGGACGGCAGATGCAGCGAAGAGGCTCAATGTTACACCACTGGCA310.15630514798568043No Hit
GAAATAGGGATACCCGGCTAATTTTGTATTTTTAGTAGACATGGGTTTTCTC310.15630514798568043No Hit
AATCAAGGGATCGTCCTGTTGTGCTCAGCCCCAACATCCCAGACCCGTTACC310.15630514798568043No Hit
TACCAGGGGGTCCTTGAATTTAACAGCAACCAACATATTGTTTGCATAGTTC300.15126304643775526No Hit
AGATATGGGGTGATGTGGTTAATGAAAAGAAGAGAACTCCAAATGAAACCAC300.15126304643775526No Hit
GTGATGGGGAGTGTAATATAAAGTGATAAATGTAGAATCGTGAAATTAGAAG300.15126304643775526No Hit
TTACAGGGGATGCCCTTACTTTAAAATAGTCCTTTTATTTTGTGTATTTTAT300.15126304643775526No Hit
TGCGCTGGGACTGTAATTAGTTTTGCAAATAACCTCTTAATTTTCAACTAGA290.1462209448898301No Hit
CAGTAGGGTGAAGAACTAAATATAAAAGTGCAATTGCAAAATACTAATTCAC290.1462209448898301No Hit
GCTTACGGGCTGATTCATGAGCACATGGTTATTACTGATCGCATTGAAAACA290.1462209448898301No Hit
GCTGCTGGTGCTCACCCTCCTCTGCAGCCTGGTGCCCATCTGTGTGCTGCGC290.1462209448898301No Hit
TGCACAGGGATCATTTAAAAAGATTTACTGCAAACTTGAAAAAGGGATTTCT280.14117884334190492No Hit
GCACATGGGAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGAACAAGG280.14117884334190492No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT280.14117884334190492No Hit
TCTACGGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT280.14117884334190492No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC280.14117884334190492No Hit
GACCTGGGGATCCTAGAAAATGTGCACCTTGATTCCTACCATTTCCATCACA280.14117884334190492No Hit
CGCAAGGGGATGTATAAGCAGTATGCTTTGATTTTTACTTAATTTAATCTTC280.14117884334190492No Hit
GAAACTGGGAGCCTACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCC280.14117884334190492No Hit
AATCTGGGGATAAGAGGAACCGTTCAGAGTCTTCTCGCAGTTCCAGAAGGCA280.14117884334190492No Hit
GTATTTGGGAAACATTAGTACTGAGGAAATAAAGAAAGGGAGAGAGGAAGAA280.14117884334190492No Hit
ATATATGGGACACATAGAAGGTGGCCACATAGGTCTCTGGGGTGAGAAGACA280.14117884334190492No Hit
TAAAAGGGGAGTTCCTTCTTTCCCTTGGACGCCCAGTGCTGGCCTTGGAGGA270.13613674179397975No Hit
GCTCTCCGGGCTCTGTCTCCCTATTCCTTCTGGTAAGAAGATAAAAGCTAAT270.13613674179397975No Hit
ATTTGAGGGATCGTTTCACTGGCACACAAAATTAATCCTTGTTAGACTGAAG270.13613674179397975No Hit
TTTTCAGGGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGTCAGGTG270.13613674179397975No Hit
ATTTACGGGGGGCTTGCTAATATTGCCCTCAGAATTAACCTACCCACCCAGT270.13613674179397975No Hit
GACAACGGGATTACTACCACTGACATGACTTTCCAAAAAACACATAATTTGA270.13613674179397975No Hit
TAGCATGGGAGTAAACTGAAACATAAAGTAAACATGCATACAAATAAAAAAC270.13613674179397975No Hit
TTTCTGGGACTCTATCTAAACTGTGAAGCTAGTAATATGGCAACAATATTAA260.13109464024605455No Hit
CTTAGTGGGTGTTTAATCTGTATGAAATGAAATTATAGCTGGGCACAGTGGT260.13109464024605455No Hit
ATTGTAGGGAAGAAATAACTGGACGAGTGCGGTATGATGTTTGCATGTGATA260.13109464024605455No Hit
AGGTATGGGATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCTGAGG260.13109464024605455No Hit
AGTTGGGGACTATAATTGGATGAGAAAATTGTTAAACAATGTCACTTTCCAT260.13109464024605455No Hit
TGTAGGGGGAACAAAAAACAGCACAAGGAAAGCCCCCAAAATATTCCAGATT260.13109464024605455No Hit
GTATGGGACTGAAAAAGCACAAAATAAATCTCCTTCTAGACTTGATTCCTTT260.13109464024605455No Hit
TTGAAGGGGATTGAATTTTGAATTTGAAGTTAAAATTATTATTTGTATTTTA260.13109464024605455No Hit
TCCAAACAGGGTGGGCCTAAATTGGGCTCTAGGTCTCTGTCCCTGGCGCTTG250.12605253869812938No Hit
AGGACCGGGACGGAGTTTGAACTCCATTCACCATCACCTTTGAGAATTTGGC250.12605253869812938No Hit
CAACCAGGGAAGGGTTCTCATTGTTGCCTTTTAAGAGGCCTAAGTCCTGCAT250.12605253869812938No Hit
TGAGCCTGGGAATCGGTGGCTGCAGCAAGCTGTGGTCACACCACTGTAATCC250.12605253869812938No Hit
GTAATGGGGCAGCTTTATTTTTCTTTAAAGCCAGTCATCATCTCTTGATAAA250.12605253869812938No Hit
AGCCCAGGTTCCCAGGGGGAGGGAAAATGGAGAAAATATAAAAAGATGTGAA250.12605253869812938No Hit
GCACTGGCACCCTTAAAATACACAAACAGTAATCATTATTCTTGTTCACATC250.12605253869812938No Hit
GCACATGGGGAAGTTTCCTGCTCTTCCATTGCAATGTTCAAACCCTTTATTG250.12605253869812938No Hit
TCTAAAGGATCTGGTGAAGGAGTCAGATGAGTAAATAAATAAGCAAGACAAA250.12605253869812938No Hit
CTAGTGGGGATATTGAAGTCGAGCAATTACTTTGCAATTTTGTACCTGACAA250.12605253869812938No Hit
CTCTGCGGGAATAGGAGATCAGCTGGAATAGGTAAGTGACAGCAACTTCCAG250.12605253869812938No Hit
GAGTAGGGGAATTCACACCATAAAGATTGTGAAGTGGTTATTGGCAAACGTT250.12605253869812938No Hit
AACCTAGGGCAATGAAGCACCTAAAAACACTGCTTGATATTATAAATTTAAA250.12605253869812938No Hit
TACAGGGGATGGATTCCAACCTTCCTTTTTTTAAATTTTCTCCAGTCCCTGG250.12605253869812938No Hit
TACGATGGGGCTATTCTAGAAGTTCTTGACAGACGAGAGAGAAGGGCAGAGT250.12605253869812938No Hit
TTGTGCGGGACGACTTTTCCTCTAAATACATCTTTATCCATATCTCTTAAAT240.12101043715020421No Hit
TTACTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA240.12101043715020421No Hit
GCGACAGGGACTTCTGACTGGCTATAAATTGGAGTTCCCATGACCCCAGCCT240.12101043715020421No Hit
GCTCATGGGATTGCAGCTATAAACCAGAGAAACAGTATCCATGTGCATAGTG240.12101043715020421No Hit
ATGACTGGGAGGAAAAATATTTAATGAAATGCAAAGACTTATAAATGAAGAT240.12101043715020421No Hit
TGCGCCGGCAAATGTGACTGAGTCATTACAGTGGACCCTCTGGGTGCATTGA240.12101043715020421No Hit
ATATCCGGGCTCTCAGCGGGAGTTTTACCACCTGAATACTGAAGCTGCCAAC240.12101043715020421No Hit
GGAAATGGGATTGGGTGTTTTTGTTTTGTTTTTACTATGAAAAGACAGCTTA240.12101043715020421No Hit
TAAGTCGGGGGTTCGAGATCAAGTTGTACAGGCTGTGCATTTTAAGATACTA240.12101043715020421No Hit
CGTACAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT230.11596833560227904No Hit
GCTTAGGGATAGCAAGACCCTGTTTCTACAGAAAATTTAAAAATTTGCCAGG230.11596833560227904No Hit
TCAAACGGGACTGGCTTGTGTTTTAGGGCACTCAATACATAAAGATGTGATT230.11596833560227904No Hit
TCACATGGGGGGCTTACATCCCCCAGGTACATACTTTTACTTATTGTGGGAT230.11596833560227904No Hit
GGCTAAGGGAGGATTAATATAAAATTCATGGAAAATGATTAATGAAGCATGG230.11596833560227904No Hit
TTTGCAGGTCAGGAGCTGAAGTCAGGATCAGAAACCACCAACTGTACTGCAA230.11596833560227904No Hit
TGCTACGGGACTGGGTATCTACCGAGAGGAAAAGAAGTCATTATACGAAAAA230.11596833560227904No Hit
GAGTGGGGGGCAAATCTGAGATTATAAAATGCCTTAGAGAATATACTAACTA230.11596833560227904No Hit
TCGCTGGGGCGTACTCTGTATATGCACGCGCTGTGCATTCTGGGGCAGCCAC230.11596833560227904No Hit
TATAACGGATTATTACTTCAGCATCAGGAAAGATTTCTTAAATAGGATATAA230.11596833560227904No Hit
ACTATGGGGGAAAAAGAAAGTAGAGAGCAGTGTGAGCTTCCCCATCAGAGTA230.11596833560227904No Hit
GCTCTAGGGATCTTGTGTGGGGATCTGGAGCCATAGTGTCCCTGAGCGATGA230.11596833560227904No Hit
AGCAGCGGAGTTACTGTAGATGAAATACAAGATGACAGCAGTGATTTGCATT230.11596833560227904No Hit
GAAATCGGGGGAAGCAGGACTGGGGGTCTGGCTTGCCCCCTCCCTGGGCCTC230.11596833560227904No Hit
AAATACGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACA220.11092623405435387No Hit
AATCCTGGGCTCTCTGCAACTTCCGCCTCCCAGGTTCAAGTGATCCTCCTGC220.11092623405435387No Hit
ACGCTCAGGGAGGATGTCCCCAGATAAATGCCCTTTAGCCTGTGACGTAAAG220.11092623405435387No Hit
CAACATGGGTCTTAAGTCGGGATTTTTAGAAGATTGTGTATTTCAAACAAAG220.11092623405435387No Hit
AGCTTGGGGACTGTGAGGATTAATTGAAATGGTTCATGAAAAGTTCCTAGTG220.11092623405435387No Hit
TAAAAAGGGCTTCTCGCCTAACGCTGCCAACATGGTGTTCAGGCGCTTCGTG220.11092623405435387No Hit
GCCTCAGGAGGACACAGCTTTTGAGGTACCATCTTGGAAGCAGAGACCAGCC220.11092623405435387No Hit
GTCAGGGGGATTTGGGAACAAAGAGAGTTTTCATCTTTTTTTCAGATCAAAA220.11092623405435387No Hit
CGAACGGGGATACTTGTCATCTATTAGTAGCTATCTAATCTACACACATACA220.11092623405435387No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG220.11092623405435387No Hit
CAACGAGGGATTCATTAAATTAGCTACTTTTTTTTTACTTTTGATAAAAGTA220.11092623405435387No Hit
GCCGCTGGGATGCTTGACACTGAGAATTCAAAGGTGAAACAAGATGGTTCTG210.10588413250642867No Hit
GAAATAGGGGCATTGATTCAGGATCTTATTGTCCATTGGGAAGGAAGATTTA210.10588413250642867No Hit
GTTCATGGGACCAGTTTCTATGAGTGGCATGGTGTGAAAAAAGTTGAGGAGC210.10588413250642867No Hit
GGTACTGGGAAATATAGTATCGTCTGCATTCCAGCAAAAATATCAACTCGTA210.10588413250642867No Hit
TCCATGGGAGTGTATCAGCGACAAACAGTTTCAGCACCTCAGTGTCTCTGCA210.10588413250642867No Hit
CTTCATTGGGGCTATCTAGAATACAGGTCTTCCAGTCAGCTGGTCATTTACC210.10588413250642867No Hit
AGATACGGGGTGGCTGTTGAGCACTAACTAGTACAGTCCAGTGCCATGGCCT210.10588413250642867No Hit
GCAAAAGGGCAGTCCACTAAAAACTAAGGGAAAGTATGTCTCCTGTAGTTAT210.10588413250642867No Hit
GACATTGGGGATCTTCTGGCGGGCGCTCTTCCGATCTGTTCGTGGGAGAGCG210.10588413250642867No Hit
TACTACGGGATATCAAAATCCAGTATTTGTAAAGCATTTAATAATATTTTTT210.10588413250642867No Hit
TTCAACGGGAATTCACTCAAAAATGTGACCGAGTAAGTATTTAGGAAGGAAT210.10588413250642867No Hit
TTCGCTGGGAGTCATTGTGTTTGCCAGTAATGTTTGAGAGACATGTAAGTTG210.10588413250642867No Hit
GGTGGGATTTCACAAAGAAATAGGATCATGAAATTGAACTTTTTTCGGACAT210.10588413250642867No Hit
GGCTAGGGGATCCTGATGAGAGAGAAGATGGTGCCAAGGCTGTCTTTGTATA210.10588413250642867No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC210.10588413250642867No Hit
TCTACGGGAGTGAGAGCCTGGCTGGGCTGGTGTCATTGGTCAGGGAAATGCA210.10588413250642867No Hit
ATAATCGGGGAGTTCTGAGCCTCTCAAGTTCCTTTCCCCAGTTAGAGTGGGG210.10588413250642867No Hit
TTTTAAGGGAACTTCTCAGGGGGCTAAAGTAGAAGGATTGCTTGAGCCTAGG210.10588413250642867No Hit
TTAAAAGGGATAGTATTCTGACTTGACAACTTAGCTAAGTTTATTAGGAATG210.10588413250642867No Hit
ACAAGAGGGATTGTTACTATTATTTATTAAAATTATTATGAGTAAAATGTTA200.1008420309585035No Hit
AGTAGCGGGTGTGTCATATGTATGTGTCATATATGTGTGTCATATATGTGTG200.1008420309585035No Hit
GCAATTGGGGAGCGAGAGTGATGATGGCTTCAGCATACACAATGCTACACTG200.1008420309585035No Hit
TCTCTGGGGGTTTTCCAGTTTTACCTTCTTTCCAGGCGGTATCCTTTGTGCA200.1008420309585035No Hit
ACTGCAGGGATGAATGTAGATCTCAGGTTAGGTTTAAGCTGACTGTCAAGTT200.1008420309585035No Hit
GCTTTGGGGACTCTGCTGCTAGGCACTCCTCTAAGCATCCTACAAATTTTAA200.1008420309585035No Hit
AAGGCTGGGAGCAGTAGAGGTTGTTCATGCTGGAAGCACATCTAGGTACACC200.1008420309585035No Hit
GATTCAGGGACTCCTCTTCCTTTTCTTCAGAGGCAGGAGAAAAGGGATTGCT200.1008420309585035No Hit
TTTTCTGGGAACTTTTTTTGGTGAAGATTCATGGTTCCTAAGTTCTGTATTC200.1008420309585035No Hit
GTGTATGGGATATGGTTCCAAGTTGATTAAGCTTTGTTACCAGCAGACTATG200.1008420309585035No Hit
TACACAGGGTGTGGTTATGATTCCAGAGTGGAATTCCCAGATGTGAGAAACT200.1008420309585035No Hit
AAACACGGGACTTAAAATGAGTTTTTAAAAGTGAAAAAGAAATGACTATATA200.1008420309585035No Hit
CATAAGGGGCTGGTAGAATTGGGTAATTGGAATTATGTAGAGCTGGAATTTT200.1008420309585035No Hit
GCTACTGGGATCATCTATCTTTCTATCACTCTATCATCTATCTATTATCTAT200.1008420309585035No Hit
GAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA200.1008420309585035No Hit
ATTACAGGGATTGAAATGTTTTGAAACTGAAAAAAAATTTTACAGCTACTGA200.1008420309585035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)