FastQCFastQC Report
Sat 31 Dec 2022
SRR5849086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179573
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15450.8603743324441869No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC14930.8314167497340914No Hit
CGGGTTGGGCCGATCTAGATAAGGGCTGCTACTTACTAGCTATGAGATCTCT2430.13532101151063913No Hit
CTTTCTGGGACTATATGGATGTTTCTAAGTGAAGGAATATGGTTCTTTTATT2360.1314228753765878No Hit
TCCATGGGGGAAATTTCACAATTCCAGAAAGCTTTAGTAATCAAAATATAGA2360.1314228753765878No Hit
ACATATGGGAAGCCTAGGTATCCTAAACACTTAGTTACTGTCACAAGTGAAG2320.12919536901427275No Hit
GTGTACGGGAGTCTAGAAAAACCAACAAGATCCCCCTTATTTAGAGATTATT2070.1152734542498037No Hit
TTTGCGGGGATTTCAGAGGACGCCATCAAGAAAAGACAAGAAGGCCGGGCAC2010.11193219470633115No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1950.10859093516285856No Hit
TTATGTGGGAGGAAGACCACAGCTAGATGGACAAACAGCATTGGGAGGCCTT1930.10747718198170103No Hit
TTCAGCGAGGGCGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTGCTC1920.10692030539112228No Hit
GAACTTGGGGTGTTCTCATTTAAATAACTTTTTTGAGATTTTAATTTTCCTG1890.10524967561938599No Hit
GAGGCTGGGAGATGGGCTGGTGTGGTTGGGTCAAGGCCCCAACACCATGGCT1880.10469279902880722No Hit
ATACAGGGGCGCTCTTCCGATCTACGTCAGGGACACTGGGGCTAGGGAAGTA1870.10413592243822846No Hit
GTGAAGGGGAGGATGGTCAGTTGATATATTAATTACAAGTCGCCCTTTTCTC1870.10413592243822846No Hit
AGTCAGGGGATGCAGTCACTGGGGCTGTGGACCTGGGACTGCCTGCAGGGTC1850.10302216925707093No Hit
TGTGAGGGGCAATGAAACCAACAACAAACATTTTTTAAAATTAAAATGGATA1840.10246529266649217No Hit
TATTACGGGCTCCGTCGACCAGATTGGGTGCTTGGCCGTCCCTGCCATTAGC1830.10190841607591343No Hit
GTTGCAGGGAATCAGTGATGCCAATATAATGAAGCTTTCATCAAGACCCAAA1820.10135153948533467No Hit
TTGGGCGGACCGGGAAATAAAGATCTTAATAAAAGCAAGCTTAATGTAGTTA1810.1007946628947559No Hit
TCGGTTGGGACTGGCAGGATGAGCTCTGTGAAGGCAGATTTATTAATGAAAA1800.10023778630417714No Hit
GTAGTGGGAGGCTGAGTCCACTTCTGCTGAGGAGTGGAGTGGGGAAGGCACC1800.10023778630417714No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)