FastQCFastQC Report
Sat 31 Dec 2022
SRR5849085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176399
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC15470.8769890985776564No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15000.8503449566040624No Hit
ACATATGGGAAGCCTAGGTATCCTAAACACTTAGTTACTGTCACAAGTGAAG2440.13832277960759415No Hit
TCCATGGGGGAAATTTCACAATTCCAGAAAGCTTTAGTAATCAAAATATAGA2230.12641795021513727No Hit
GTGAAGGGGAGGATGGTCAGTTGATATATTAATTACAAGTCGCCCTTTTCTC2200.12471726030192916No Hit
TTATGTGGGAGGAAGACCACAGCTAGATGGACAAACAGCATTGGGAGGCCTT2180.12358346702645708No Hit
CGGGTTGGGCCGATCTAGATAAGGGCTGCTACTTACTAGCTATGAGATCTCT2170.12301657038872103No Hit
CTTTCTGGGACTATATGGATGTTTCTAAGTGAAGGAATATGGTTCTTTTATT2160.12244967375098499No Hit
GAGGCTGGGAGATGGGCTGGTGTGGTTGGGTCAAGGCCCCAACACCATGGCT2150.12188277711324895No Hit
TATAAAGGGGTAGAACACACTAAATATAACAAACAATAACTATTACTCTGTC2070.11734760401136062No Hit
TTTGCGGGGATTTCAGAGGACGCCATCAAGAAAAGACAAGAAGGCCGGGCAC2010.11394622418494436No Hit
TCGGTTGGGACTGGCAGGATGAGCTCTGTGAAGGCAGATTTATTAATGAAAA1990.11281243090947228No Hit
GAACTTGGGGTGTTCTCATTTAAATAACTTTTTTGAGATTTTAATTTTCCTG1930.10941105108305603No Hit
AGTCAGGGGATGCAGTCACTGGGGCTGTGGACCTGGGACTGCCTGCAGGGTC1910.10827725780758395No Hit
ATACAGGGGCGCTCTTCCGATCTACGTCAGGGACACTGGGGCTAGGGAAGTA1910.10827725780758395No Hit
GGTGCAGGGATATACGCACAGGTCAAAGAGAATGAACTCTAAATGTTTCCCA1850.1048758779811677No Hit
GTTGCAGGGAATCAGTGATGCCAATATAATGAAGCTTTCATCAAGACCCAAA1840.10430898134343165No Hit
TTCAGCGAGGGCGGAGGCAGCCATGGTGCGGTTCAAGCACAGGTACCTGCTC1790.10147449815475144No Hit
GTCCTGGGGGTTCCTCTTCGTGCGCGACCCCTTCGTGCGCCTGATCTCCGCC1780.1009076015170154No Hit
TGCCTGGGGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCC1770.10034070487927936No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)