FastQCFastQC Report
Sat 31 Dec 2022
SRR5849084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180182
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT14700.8158417599982241No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC14650.8130667880254409No Hit
TCCATGGGGGAAATTTCACAATTCCAGAAAGCTTTAGTAATCAAAATATAGA2480.1376386098500405No Hit
ACATATGGGAAGCCTAGGTATCCTAAACACTTAGTTACTGTCACAAGTGAAG2310.12820370514257806No Hit
CGGGTTGGGCCGATCTAGATAAGGGCTGCTACTTACTAGCTATGAGATCTCT2250.12487373877523839No Hit
TATAAAGGGGTAGAACACACTAAATATAACAAACAATAACTATTACTCTGTC2230.12376374998612513No Hit
GTGAAGGGGAGGATGGTCAGTTGATATATTAATTACAAGTCGCCCTTTTCTC2210.1226537611970119No Hit
TTATGTGGGAGGAAGACCACAGCTAGATGGACAAACAGCATTGGGAGGCCTT2120.11765881164600238No Hit
TTGGGCGGACCGGGAAATAAAGATCTTAATAAAAGCAAGCTTAATGTAGTTA2010.11155387330587961No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC2010.11155387330587961No Hit
ATACAGGGGCGCTCTTCCGATCTACGTCAGGGACACTGGGGCTAGGGAAGTA2000.110998878911323No Hit
TTTGCGGGGATTTCAGAGGACGCCATCAAGAAAAGACAAGAAGGCCGGGCAC2000.110998878911323No Hit
GTTGCAGGGAATCAGTGATGCCAATATAATGAAGCTTTCATCAAGACCCAAA1980.10988889012220977No Hit
CTATCTGGGAACTGGGAATAATTTTATTTCAGATTTTACTAATGTAGTATGT1920.10655892375487008No Hit
GAGGCTGGGAGATGGGCTGGTGTGGTTGGGTCAAGGCCCCAACACCATGGCT1920.10655892375487008No Hit
CTTTCTGGGACTATATGGATGTTTCTAAGTGAAGGAATATGGTTCTTTTATT1900.10544893496575686No Hit
GTTGCAGGGATTGTCCTAAGAAGTATGTCACCAAAAGTAATTCCATTTTTTT1890.10489394057120022No Hit
ATTGCGGGGCTGCCCACTTGCTTACATAAACAGAAATTTAACAGCCCATATT1890.10489394057120022No Hit
TACGTTGGGGTCAGGCTTCCTGGAGGCATCAGGCTTTGAGCCCTGCTGGGGT1880.1043389461766436No Hit
GTAGTGGGAGGCTGAGTCCACTTCTGCTGAGGAGTGGAGTGGGGAAGGCACC1840.10211896859841717No Hit
TGTGAGGGGCAATGAAACCAACAACAAACATTTTTTAAAATTAAAATGGATA1840.10211896859841717No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)