FastQCFastQC Report
Sat 31 Dec 2022
SRR5849082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168420
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT19271.1441634010212565No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC17971.0669754185963662No Hit
CATTCTTCTAGAGATCAGTGAAGGAGCTGATTCTTTTATTACTTCATATAGC2070.12290701816886355No Hit
ATGGTAGGGTGTTATTCACAGACAATTGTCTTGTTTCAATCCTTTTCAAAAG2010.11934449590309941No Hit
GTGCTAGGGGAAAAGAAAAAGGCAGAAATAATATTTGAGGAAATAATGGCTG1930.11459446621541385No Hit
CTTCTCGGGGTAGCCTTGGTCATATCAAGTTTCTGCTGAAAAATTTGGCATG1810.10746942168388551No Hit
CTTTTAGGGATATTATTAAATGTTGATTGATGGCTTGTAATGTACTGCACAC1780.10568816055100344No Hit
ATGGCCGGGATGGATACTATTTCTAGTAATGTAATTTTTATCAGAATTGCCA1740.10331314570716067No Hit
ACAATGGGACATTGATCATGGCGGATTCTTTTAAGAATCCTTGGCCTTTTCC1740.10331314570716067No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1730.10271939199619998No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)