FastQCFastQC Report
Sat 31 Dec 2022
SRR5849081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171518
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT18541.08093611166175No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC17411.0150538135939084No Hit
CATTCTTCTAGAGATCAGTGAAGGAGCTGATTCTTTTATTACTTCATATAGC2160.1259343042712718No Hit
ATGGTAGGGTGTTATTCACAGACAATTGTCTTGTTTCAATCCTTTTCAAAAG1950.11369069135600929No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC1930.11252463298312713No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1810.10552828274583426No Hit
AAGCATGGGAGAGCCACTGAATATTAAAAATGCCCATAGGGAAAAGCAGGAA1810.10552828274583426No Hit
ACAATGGGACATTGATCATGGCGGATTCTTTTAAGAATCCTTGGCCTTTTCC1730.10086404925430568No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)