FastQCFastQC Report
Sat 31 Dec 2022
SRR5849080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50861
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC16613.265763551640746No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT16393.222508405261398No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3230.6350641945695129No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1940.3814317453451564No Hit
CATCAAGGGTCACAACTGAAAGTATGAACTATTGAAAAATTAATCATGGCTG1850.36373645818996875No Hit
TTCAAGGGGATAGAAAATGTCTGACTGTTTGCAAAATAATATTCTGTTTTAA1670.3283458838795934No Hit
ATTAACGGGCCGGCGAATCAACATGGTAGAAGAGCTTGAGAAAGCCCTGGGG1650.32441359784510726No Hit
TAACTCGGGCACGGGGACCTGATCCGGGGCACGGTGCTCACCTACCAGTGCG1610.316549025776135No Hit
AATTGTGGGAGTCACAGTGGTGATAACATATGCTTTCAGATTTACGCAGCAT1530.30081988163819035No Hit
GACCAGGGGTACAGAGGGGGCTGCAGTGTACCCTCACGAGGGACAGCCAGGA1500.29492145258646113No Hit
TGCCGGGGGTTTGTTACTTGGACTAACAAGGCAGTAGATTTGCCTGTGTGGA1470.2890230235347319No Hit
GCATATGGGTAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAA1470.2890230235347319No Hit
AGTACTGGGAGAGGAATTAATTAAGTGGATATATACTATATTCATGGATAGG1450.28509073750024577No Hit
GTGCTGGGATTACAGGCGTGAGCCACCGCACCCAGCCATGCCTGGAATATTT1440.2831245944830027No Hit
TTATGTGGGGGCCGCTTCTCCCTGCCCCGCAGCGCGCAGGGACCATGTCGGC1440.2831245944830027No Hit
CAATAGGGGATTCACATTTTTGCCCATTTTTTAGTTGGGTTGTCTTTTTTTG1440.2831245944830027No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1420.27919230844851656No Hit
CTCGTAGGGTAGCCGGCTGGAGTACGGGGCCTTCGGGAGCCGCGCGCCTTCT1410.2772261654312735No Hit
TCATTTGGGGCCAGATTTTCCTGCTTTAGTACTTGGAGAAAGTATAGCAGCA1410.2772261654312735No Hit
TGGACTGGGGTTAGAGTAAATCTATTGTGTAATTTTACATGGCATTTATATT1380.27132773637954427No Hit
AATACCGGGAGGCGCTAAGAGGCACGCTGAGTAAACTGATTTCCCACCGGGG1380.27132773637954427No Hit
AAAGTAGGGCACATTCACTGAATTCACTGAGATTATTCCTATAATTTTAAAG1350.26542930732781506No Hit
ACATTTGGGGAAGGGAAATATGTTTTTAGAAACCCTTATTAAATACTACAAT1330.2614970212933289No Hit
ACCCGGGAGCATTTATTCTTGTGGATTTAATATGAATTTTAAAAACCACTTG1310.2575647352588427No Hit
CATGTGGGGTAATCACAAACCCCAAAACGGGAGAGAGCAGTGAAAACATGCA1280.2516663062071135No Hit
TTGAGAGGGGCTCCGGCCCAGCATGGCGACCCCGACCCAGACCCCCACAAAG1260.24773402017262736No Hit
AAGTGGGGTGATGAGAGTGTCTATGGGAAATGAGTGGTTGGAAGCCCAGCAG1260.24773402017262736No Hit
CGCTGCGGGACGGTCTCTTTGGGTAGATATGTAGCTGCACAAGCAAATATAA1250.24576787715538428No Hit
TGAAAAGGGTGGATATATATAGTATTGGGTGATGGTGCAGAATACAAAAGTT1220.23986944810365507No Hit
GCCCGAGGGGATACACTACCTCTTACGTGTTGCCTCTTTGTGTTGCTTGGTG1200.23593716206916893No Hit
ACTCATGGGTATGGCTTTATTTTTGGAGGCTTAAAAAATTAAAAACTCTAGA1170.2300387330174397No Hit
GCATTGGGATAATTTTAGACTTACAGAAGAGTTGTAAAAGTAGTAGAGTTCT1140.22414030396571047No Hit
TAGAAGGGATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATC1130.22217416094846737No Hit
TTAGGAGGGCCAGTTAACATTTAGGGAGTTATCTGTTTTCATCTTGAGGTGG1130.22217416094846737No Hit
TACTCCGGGGTCAGTATAAAAGTGCCAAACCTGCCCTCTTTTAAATAAAAAT1120.22020801793122433No Hit
CAGACCTGGGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGAC1120.22020801793122433No Hit
TTGTCGGGGAACTCAAGGCACTGATTTCTGCATCTTCAGCCTCCTCATTTCA1110.21824187491398125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1090.2143095888794951No Hit
ACACGGGATTTTGAGCGCTAATAAGCAAAAGAGCATGCAGTGCTGTTGAATA1080.212343445862252No Hit
TCTGGGGAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAA1070.21037730284500894No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT1050.2064450168105228No Hit
TGTCGTGGGAGTAGTGTGGAAACTTAGAGAACTTATTGTATGGTGCCTTGCA1050.2064450168105228No Hit
AGTTAGGGGCCTTAGAGCTTGAGCTGCCAGAGTCCCCTGTGAATCAAGATTT1040.20447887379327975No Hit
ATTGGTGGGATTCATACTTTTTTAACAAGCTTTTGTTATATATTTTCTAATC1030.20251273077603665No Hit
GTTCTGGGGAACTCCTTTGTGCCTCAAGATCCTTTTTTTCTGTTCCTTTTCA1030.20251273077603665No Hit
GCACTTGGGAAGAAAACACTGAATACGTAACTTTAAAGGATCCCTGAAGAAA1000.1966143017243074No Hit
TACGGCGGGAGCTCTCAGGCACAAATCAACATAACCACTATTCTAAAAGCTA990.19464815870706434No Hit
TTTACTGGGAGATATATTTTAAACGTGCCTTAATAGCTCAAGCTAGACTTCT990.19464815870706434No Hit
CTTGGGTTGGGTCGAGCCAAATCGCATGGCTTTCAGATCAGGTATTCTGCAC980.1926820156898213No Hit
TGATAAGGGTACGGAGGAATGATTGTTTAAACCATTGCCACTGGTGATCAAC970.1907158726725782No Hit
GGTGAGGGTAAGAGTCTGGAGGACACCTGTATATTTATACTGTCCATTTCGG970.1907158726725782No Hit
TTGCTTGGGCAAGGCACACATTCTACAGCAAGTCATAGAAAGTTTAATCCTT960.18874972965533512No Hit
GCTCGTGGGATAGGAATTCCTGGGACCTCTTTGACTTTTAACTTCTTATACA960.18874972965533512No Hit
TAAAGCGGGGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTT960.18874972965533512No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT950.18678358663809205No Hit
GCCATGGGCGCTCTTTCCGATCTCATTAGGGGTAAAGAAGTGAGGGCTGTGG930.1828513006036059No Hit
GCGAACTAGGCAGAAAGTAATTGTCACTCATTCACACACAGAACTTCCATCA920.18088515758636284No Hit
GAAAAAGGGTGGATTAGTACCATGGTCTGACTTGAAAGTGTTTTCGGTAAGT900.1769528715518767No Hit
ATAGAGGGGGTTACATCCACCAACAAAACACTAGGATTCCAATTTTTCCACA900.1769528715518767No Hit
GGTCATGGGAGAGGTTAGAGATTTAAAACTCTAGTAACTTATGTATTATAAT900.1769528715518767No Hit
CTAATTGGGATTTAAAACAGTGGCATCTGATTTATAAGGTGGCATTTGGGAT880.17302058551739052No Hit
TCGATAGGGTGGTGCAAAAGTGATCCGCATTCAGTAGAAACTGTTCCTCAAG880.17302058551739052No Hit
GTTAAAGGGATGAGTACGGTGTGTTTCTGAGAGAGTGTGATGTTCACCTCTG870.17105444250014745No Hit
ATTTGAGGGAAGGTGGGCTTGGTCCAACAGGTTCCCAGAGGGTACATACTCC870.17105444250014745No Hit
CTCTCTGGGGCTCTATAGAAATGAAAATGCAGCATGAAGTTGACATTGTGGA860.16908829948290438No Hit
GTAACTGGGAATGTTGGCATATATTATACTTTTCAAAAGCAGTTTCCACTAA860.16908829948290438No Hit
TAAGCGGGGAATAAGCAAATATTGTCTTCAAAAGAATGCACAAGAACCACAA860.16908829948290438No Hit
TATGAAGGGGGAGATGATACAGGATGGCCACCAGGTGTCTTTGTTAAGCGGG850.1671221564656613No Hit
TCGTCAGGGATTACTTGCACCAGAAATGATTTCAAACACTTTAGGAACTGTG850.1671221564656613No Hit
CAAAAGGGGAAGTTTCTTACTACAGGTAAAAGCTATGTGCAAGAACCTCAAA840.16515601344841824No Hit
ACGTTTGGGATTCATATTGTTTCTGGAAGAGGAAGAATTGGTTAAACAATAG830.16318987043117517No Hit
TTTATAGGGAGTGTTAAATATGTGTGCATAAAGTCTTAAATGAAAAGATGTG830.16318987043117517No Hit
GCGCTGGGAGATCTGTTTACTAGCCAATGAGCTGTTTGATGTCTGAGGGAAA830.16318987043117517No Hit
TAATCAGGGAGCTGAATTCACATATCTGATGAGATTAGACAGTTTATCAAAA810.15925758439668902No Hit
AACTTTGGGACCAGATTAAGTCATGTATTGACCAGTGAGCAAGTATACGCCA800.15729144137944592No Hit
ACAATTGGGATTTTTTCCCCTGGACTATACTTAGAAATACTACATCAATTGT800.15729144137944592No Hit
ACACAGGGACAATAATATTCAACAGAACTTAACCTGGACCTTATGTTGTAAT800.15729144137944592No Hit
CTCCCCGGGCATTTCCAGCTCTTCACTTTTTGGCACTGGATGCATCTCAGCA800.15729144137944592No Hit
TGAACTGGAAGAGGTATGGGAAAAATGGAACTAGGATGGTGGATAAAGGGCA800.15729144137944592No Hit
GAAGGTGGGTGCATATAAAAACTATAAATGCCGAGTGGTAGAAACTCCTCTT790.15532529836220285No Hit
AACCTTGGGCTGGTGCTGCTTCATAACAAAAATGAAAAGCTGCATGCATCTA780.1533591553449598No Hit
TAGTACGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT780.1533591553449598No Hit
AAGGCTGGGAAGGCAGAATGTTCCCTGACACCAGTGTGTGGATTTTTAACAT770.15139301232771674No Hit
GTATGTGGGAACACCCTAGTTTTCTGAACTGCAGCTGCATGGGTAATTATCT770.15139301232771674No Hit
AAGTGGGAGAGGTTTGGTAGTAACAACACTTCTGGATCTTAAGGCTTCATAA770.15139301232771674No Hit
CTGGTTGCGGGATGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGG760.14942686931047364No Hit
GTCATAGGGGTTTCAAGAGAAGAATTTGTTTACACATAAAGACAGATGCTTA760.14942686931047364No Hit
CACATCGGGGAGGGCTCAGCTCGCATGTGGAAGACTCTCACCTTCTTCGTCG760.14942686931047364No Hit
ACATTAGGGTTGCGGGGGCAGATTAAAAAATTAGAAGGAAATTTCAGTGGAT740.1454945832759875No Hit
TTAAGGGGAAGCAGCAAAGCATTTAAGAGGTGGCCTGGCTGATTCTGAAAGC740.1454945832759875No Hit
AGTCACGGGATGTAAAAACCTGGATGTTAACCTCCAAGACATTGTTCACGTC740.1454945832759875No Hit
CGATCCGGGTAGTTGGGTAATTCCAAGTGCTGATAGTACTATTCATCTTTTT740.1454945832759875No Hit
TTAGAGGGAAACAATCTTTGAAGAATTTCTGAAACTGTTTACAAGGAATTTT730.14352844025874442No Hit
ATAGCCGGGAGACAATCCACAATGTATACATATAGCAAAACATCATGTCGTA730.14352844025874442No Hit
GCAGAGGGGATTTGTAGATTGCAAGTTTTGTTTAAAATAACTTTATTGAGTT700.1376300112070152No Hit
AGTTTAGGGACTGTGTTACAATAATGGCAAGTAACATGTTAAGAAGGTTTAC690.13566386818977214No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTC690.13566386818977214No Hit
GCCATGGGCGCTTCCGATCTTAGCTAGGGAATCCAGATGTAAACAACCAGAT690.13566386818977214No Hit
TTTGTCGGGGTTAGTGACTGATAAGGATGCCTTTGTTTTGTACATGAACCAA690.13566386818977214No Hit
AAAACGGGGTGTCATTGAAACTACTCTAATGCTGTAAAAGTGGCACTGCTAA680.13369772517252904No Hit
ATTGGGGGGAGCGCAGGCGCGGTGGCGCGAGTTGGACTGTGAAGAAACATGG680.13369772517252904No Hit
AAGTACGGGATTCCCATTATCTTTGACCAGTATTAATTTTTGAGATCTTACT680.13369772517252904No Hit
TATGGAGGGGTCATCACCATGTAAGTCCCAAGATCAGTCCCGTAATATATTG680.13369772517252904No Hit
AGCAAGGGGATCAGTAAAGGTATTGCTGCTGGAATGAAGAAAATGGGATACG680.13369772517252904No Hit
GTCAAAGGGGCCAGGACATGTTTTCTTAAAGACAATTTAAGAGTCAGGAAGT670.13173158215528596No Hit
ATCTTAGGGAGGAGGCTGAGGTGGGAGAATTGCTTGAACCTGGGAGGCGGAG670.13173158215528596No Hit
CTTTGGGGGGAAGTTTTTGCAATTCTAGAACTTGGATTTTTTTTTAACAAAA670.13173158215528596No Hit
TACAGGGGTGGTGTTGGTCAGGAAAACTAGATTGATGGGCTATCTTGCCGTG670.13173158215528596No Hit
ACTAGCGGGAGATGTGTCAGAAACATAGTCAGGATGTGCCATTAACTTTGAT670.13173158215528596No Hit
AGATCAGGGAAAGAGAATATCATGAAAATCAGGAAGGATTACACCATTGAAG660.1297654391380429No Hit
TTGTGTGGGTATCCATTGACTTCTAATCTTCTGCTAATGGTGATGTGAATTC660.1297654391380429No Hit
CTCTGTGGGATGGATTTAGAAAAAAAGATCTGTGATGAAAACATTCATTGAT660.1297654391380429No Hit
AGAGTTGGGACGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTC660.1297654391380429No Hit
CTAGGCGGGCCATTTACCTAATTTTACTAGTGGATTTTATGCCTTCAAATGT660.1297654391380429No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC650.12779929612079982No Hit
CCAAAAGGGAGAATAATTGATTTTTATCATCTTTCTTAGTATACCTTTCACG640.12583315310355675No Hit
CTGCTAGGGATGTGATAAATCACATTTATTGATTTGAATATGTTGAACCAAC630.12386701008631368No Hit
TGCAGAGGGTGACACAACCACTTTGGAAAAATATGTGGCAGGTTCTTAAAGT630.12386701008631368No Hit
AACGCAGGGGAAAAGATCAAACGGTGAAGTGAAGAAATGATATGAAATGATG630.12386701008631368No Hit
ACTAGGGTGCCCCGCGGCCCTGCTGCTGGCCCCGGGGATCTGAGTCGTACCC630.12386701008631368No Hit
GCATAAGGGATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATAT630.12386701008631368No Hit
GATACGGGGCCCGAGGCCCTGCGCTCTCGGCCGGGCTCCCCAGCCCTCTGCT630.12386701008631368No Hit
CGGTATGGGTGTGACACCTTTATATGAATTATTTTCCCAATTTTTGCTCTAT620.1219008670690706No Hit
CATTGCGGGAATATTCTATTTGGTATTATATTATTTGATGTTTGCTGTTCTT620.1219008670690706No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTAT620.1219008670690706No Hit
TTGCTGGGAGCCCTCTGCTTTTTAACATGCAAGATGTATCAGTCGTTGGTTG620.1219008670690706No Hit
CATATTGGGTACCTTGATTCGTATCCTAATGTCTGATGTTATTGGTAGTTCT610.11993472405182753No Hit
TAATGTGGGGGATCTATAAATATGCTAAAGCCATTTCTATCCTTTATTTTTA610.11993472405182753No Hit
ACATGAGGGATGGCCCTTTACAATTTTTATTGCATAGTGCAATATAATAGCA600.11796858103458446No Hit
GAGTCTGGGACTCACTGCACATCATAAATCTCCATCATCTGTCTTAAAGTTT590.11600243801734138No Hit
AGGTTTGGGCCTGCTTAAGTGGAGAAATAGTAGACTTGAACCAATTGAAAAA590.11600243801734138No Hit
GTCGATGGGTGCTGCAATATAGATCAAAGATCTTGTGGCATAGGGGTAGGGG590.11600243801734138No Hit
CAAACTGGGACTTATTTTCTGAATAATTATATATGAATGCCCAAAAATAAAC590.11600243801734138No Hit
AAGAGAGGGATGGCTGGTTGTGGTGGCTCATGCCTGTAATCCCCGCGCTTTG590.11600243801734138No Hit
GTGGTTGGGCTCTTGTAAGAAATGTATTAGGCCAGGTGAGGTGGTTCATGCC590.11600243801734138No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC580.1140362950000983No Hit
CTTCTAGGGAATTGGGAAATTCATGCTAACAAATATTAATTGCTCTTTCTTC580.1140362950000983No Hit
TTAGCTGGGATGACATAGAAATGGCTATGTAAACTATAATTATTTTGCTAAG580.1140362950000983No Hit
CACGACGGGGACGCTCTTCCGATCTCTTTAGGGGATCTGTTCTCCAGTTGAA580.1140362950000983No Hit
TCATCCGGGGAATCACAGAATTTGTCCTTTTGTGACTGGCTAATTTCTTTTC580.1140362950000983No Hit
GACTTTGGGAAACCAAGCATTGAATAATGGCTGGATAACTGCCGAAGTAAGC570.11207015198285523No Hit
CCGATGGGGGTCACAATTCCTCAATCTGAAACAATGAGTTAAATATAATAAC550.10813786594836908No Hit
TCAATAGGGGTGACGTGTTGTAGTTTTTAGATGTTTGTAAAATGTTTAAAAA550.10813786594836908No Hit
TTAGTAGGGCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATC550.10813786594836908No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACCTCGTATGC540.106171722931126No Hit
GAGCCGGGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCG540.106171722931126No Hit
CTAAATGGGATGAAAATTTTCTGGTTTGCACCATGAATTTGTCAAATGGATA540.106171722931126No Hit
CGTTTGGGGAGGTTTAAGAAAAAATTTTCTTGTCTGAAAACATAAACATCTT540.106171722931126No Hit
TGTGAAGGGGAACTAAAGTATTTTTCTTAAGCCTATTGAGTGATTTATTTTT540.106171722931126No Hit
TATGCAGGGAACAAAACTGAAGCAAGAAACTGTAGAGATGGACATTAGAATT530.10420557991388293No Hit
GCGTTGGGGATATATTTATAAGTTTAATGATATTCTCAACAAAATTCCAATG530.10420557991388293No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT520.10223943689663988No Hit
ATACTGGGTCCACCTCCAGTGCACTTATAAACTCCTACACAGTATATAAGAT520.10223943689663988No Hit
TTGCATGGGATGAAGGATCAAAAAATGTTTTTCAATGTGTTCTTTGTTCCAC510.10027329387939678No Hit
GAACGTGGGATACCAATGAGAGCCTCACACATTCAAGGATGTACTAAGCACT510.10027329387939678No Hit
CTAAGCGGGAATGTTATCTGGATCCCTATCTGTGAGAGTTGCTCTTCTCTCT510.10027329387939678No Hit
CCCTTGGGGAACTTTGAAGTGTTTGTACAGTAATGATCTTTGCTGACTGATT510.10027329387939678No Hit
GCAAATGGGGATTACTCCTGCCATCATGACCCTTGGCCATAATATGATTTAT510.10027329387939678No Hit
GTCATCGGGAATGTTTATATATATATTTTTAAATTACTGAATTTCAAAATAT510.10027329387939678No Hit
CGACGCGGGTATCCTCAAGTTGTGTGCTATTCGTAAGTTCTGTGCAGTTTGG510.10027329387939678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)