FastQCFastQC Report
Sat 31 Dec 2022
SRR5849079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50271
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT16453.2722643273457854No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC16313.2444152692407155No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3230.6425175548526983No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1940.3859083765988343No Hit
ATTAACGGGCCGGCGAATCAACATGGTAGAAGAGCTTGAGAAAGCCCTGGGG1770.35209166318553436No Hit
AGTACTGGGAGAGGAATTAATTAAGTGGATATATACTATATTCATGGATAGG1680.3341886972608462No Hit
TTATGTGGGGGCCGCTTCTCCCTGCCCCGCAGCGCGCAGGGACCATGTCGGC1670.33219947882476974No Hit
GACCAGGGGTACAGAGGGGGCTGCAGTGTACCCTCACGAGGGACAGCCAGGA1620.3222533866443874No Hit
CAATAGGGGATTCACATTTTTGCCCATTTTTTAGTTGGGTTGTCTTTTTTTG1620.3222533866443874No Hit
TAACTCGGGCACGGGGACCTGATCCGGGGCACGGTGCTCACCTACCAGTGCG1580.31429651290008154No Hit
TTCAAGGGGATAGAAAATGTCTGACTGTTTGCAAAATAATATTCTGTTTTAA1560.3103180760279286No Hit
CGCTGCGGGACGGTCTCTTTGGGTAGATATGTAGCTGCACAAGCAAATATAA1540.3063396391557757No Hit
TTGCTTGGGCAAGGCACACATTCTACAGCAAGTCATAGAAAGTTTAATCCTT1500.29838276541146985No Hit
TTGAGAGGGGCTCCGGCCCAGCATGGCGACCCCGACCCAGACCCCCACAAAG1480.2944043285393169No Hit
CATCAAGGGTCACAACTGAAAGTATGAACTATTGAAAAATTAATCATGGCTG1480.2944043285393169No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1450.2884366732310875No Hit
TGCCGGGGGTTTGTTACTTGGACTAACAAGGCAGTAGATTTGCCTGTGTGGA1410.28047979948678164No Hit
TGGACTGGGGTTAGAGTAAATCTATTGTGTAATTTTACATGGCATTTATATT1400.2784905810507052No Hit
AATACCGGGAGGCGCTAAGAGGCACGCTGAGTAAACTGATTTCCCACCGGGG1370.2725229257424758No Hit
TCATTTGGGGCCAGATTTTCCTGCTTTAGTACTTGGAGAAAGTATAGCAGCA1350.26854448887032284No Hit
GTGCTGGGATTACAGGCGTGAGCCACCGCACCCAGCCATGCCTGGAATATTT1320.26257683356209344No Hit
AATTGTGGGAGTCACAGTGGTGATAACATATGCTTTCAGATTTACGCAGCAT1320.26257683356209344No Hit
ACCCGGGAGCATTTATTCTTGTGGATTTAATATGAATTTTAAAAACCACTTG1310.26058761512601697No Hit
TAGAAGGGATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATC1290.25660917825386403No Hit
TTAGGAGGGCCAGTTAACATTTAGGGAGTTATCTGTTTTCATCTTGAGGTGG1280.2546199598177876No Hit
GCATATGGGTAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAA1280.2546199598177876No Hit
GCATTGGGATAATTTTAGACTTACAGAAGAGTTGTAAAAGTAGTAGAGTTCT1260.25064152294563463No Hit
ACATTTGGGGAAGGGAAATATGTTTTTAGAAACCCTTATTAAATACTACAAT1260.25064152294563463No Hit
ATTGGTGGGATTCATACTTTTTTAACAAGCTTTTGTTATATATTTTCTAATC1210.24069543076525235No Hit
ACACGGGATTTTGAGCGCTAATAAGCAAAAGAGCATGCAGTGCTGTTGAATA1200.23870621232917588No Hit
AAAGTAGGGCACATTCACTGAATTCACTGAGATTATTCCTATAATTTTAAAG1180.23472777545702292No Hit
AAGTGGGGTGATGAGAGTGTCTATGGGAAATGAGTGGTTGGAAGCCCAGCAG1150.22876012014879354No Hit
CTCCCCGGGCATTTCCAGCTCTTCACTTTTTGGCACTGGATGCATCTCAGCA1130.22478168327664058No Hit
ACAATTGGGATTTTTTCCCCTGGACTATACTTAGAAATACTACATCAATTGT1120.22279246484056414No Hit
CTCTCTGGGGCTCTATAGAAATGAAAATGCAGCATGAAGTTGACATTGTGGA1080.2148355910962583No Hit
AGTTAGGGGCCTTAGAGCTTGAGCTGCCAGAGTCCCCTGTGAATCAAGATTT1080.2148355910962583No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1070.21284637266018183No Hit
TTGTCGGGGAACTCAAGGCACTGATTTCTGCATCTTCAGCCTCCTCATTTCA1060.21085715422410534No Hit
GTATGTGGGAACACCCTAGTTTTCTGAACTGCAGCTGCATGGGTAATTATCT1050.20886793578802887No Hit
ACTCATGGGTATGGCTTTATTTTTGGAGGCTTAAAAAATTAAAAACTCTAGA1050.20886793578802887No Hit
CATGTGGGGTAATCACAAACCCCAAAACGGGAGAGAGCAGTGAAAACATGCA1050.20886793578802887No Hit
GCACTTGGGAAGAAAACACTGAATACGTAACTTTAAAGGATCCCTGAAGAAA1040.2068787173519524No Hit
CTCGTAGGGTAGCCGGCTGGAGTACGGGGCCTTCGGGAGCCGCGCGCCTTCT1040.2068787173519524No Hit
CTAGGCGGGCCATTTACCTAATTTTACTAGTGGATTTTATGCCTTCAAATGT1040.2068787173519524No Hit
TACTCCGGGGTCAGTATAAAAGTGCCAAACCTGCCCTCTTTTAAATAAAAAT1030.20488949891587596No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT1030.20488949891587596No Hit
CAGACCTGGGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGAC1020.2029002804797995No Hit
TGTCGTGGGAGTAGTGTGGAAACTTAGAGAACTTATTGTATGGTGCCTTGCA1020.2029002804797995No Hit
TGATAAGGGTACGGAGGAATGATTGTTTAAACCATTGCCACTGGTGATCAAC1010.20091106204372303No Hit
GCGAACTAGGCAGAAAGTAATTGTCACTCATTCACACACAGAACTTCCATCA1000.19892184360764653No Hit
TCGTCAGGGATTACTTGCACCAGAAATGATTTCAAACACTTTAGGAACTGTG980.19494340673549362No Hit
ATAGCCGGGAGACAATCCACAATGTATACATATAGCAAAACATCATGTCGTA980.19494340673549362No Hit
GAAAAAGGGTGGATTAGTACCATGGTCTGACTTGAAAGTGTTTTCGGTAAGT970.19295418829941716No Hit
TAAAGCGGGGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTT950.18897575142726422No Hit
GTTCTGGGGAACTCCTTTGTGCCTCAAGATCCTTTTTTTCTGTTCCTTTTCA940.18698653299118775No Hit
GCTCGTGGGATAGGAATTCCTGGGACCTCTTTGACTTTTAACTTCTTATACA930.18499731455511131No Hit
ACACAGGGACAATAATATTCAACAGAACTTAACCTGGACCTTATGTTGTAAT930.18499731455511131No Hit
TCGATAGGGTGGTGCAAAAGTGATCCGCATTCAGTAGAAACTGTTCCTCAAG920.18300809611903482No Hit
TGAAAAGGGTGGATATATATAGTATTGGGTGATGGTGCAGAATACAAAAGTT920.18300809611903482No Hit
ACTAGCGGGAGATGTGTCAGAAACATAGTCAGGATGTGCCATTAACTTTGAT910.18101887768295835No Hit
GCCCGAGGGGATACACTACCTCTTACGTGTTGCCTCTTTGTGTTGCTTGGTG900.1790296592468819No Hit
GATACGGGGCCCGAGGCCCTGCGCTCTCGGCCGGGCTCCCCAGCCCTCTGCT900.1790296592468819No Hit
TATGAAGGGGGAGATGATACAGGATGGCCACCAGGTGTCTTTGTTAAGCGGG890.17704044081080542No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT880.17505122237472898No Hit
CTTGGGTTGGGTCGAGCCAAATCGCATGGCTTTCAGATCAGGTATTCTGCAC880.17505122237472898No Hit
GCGCTGGGAGATCTGTTTACTAGCCAATGAGCTGTTTGATGTCTGAGGGAAA880.17505122237472898No Hit
TAAGCGGGGAATAAGCAAATATTGTCTTCAAAAGAATGCACAAGAACCACAA860.17107278550257604No Hit
TCTGGGGAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAA860.17107278550257604No Hit
GTTAAAGGGATGAGTACGGTGTGTTTCTGAGAGAGTGTGATGTTCACCTCTG840.1670943486304231No Hit
TAATCAGGGAGCTGAATTCACATATCTGATGAGATTAGACAGTTTATCAAAA840.1670943486304231No Hit
CTGGTTGCGGGATGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGG820.16311591175827017No Hit
GCCATGGGCGCTCTTTCCGATCTCATTAGGGGTAAAGAAGTGAGGGCTGTGG820.16311591175827017No Hit
GGTGAGGGTAAGAGTCTGGAGGACACCTGTATATTTATACTGTCCATTTCGG820.16311591175827017No Hit
ATTGGGGGGAGCGCAGGCGCGGTGGCGCGAGTTGGACTGTGAAGAAACATGG800.15913747488611726No Hit
CTAATTGGGATTTAAAACAGTGGCATCTGATTTATAAGGTGGCATTTGGGAT800.15913747488611726No Hit
TCAATAGGGGTGACGTGTTGTAGTTTTTAGATGTTTGTAAAATGTTTAAAAA800.15913747488611726No Hit
GCCATGGGCGCTTCCGATCTTAGCTAGGGAATCCAGATGTAAACAACCAGAT800.15913747488611726No Hit
AACTTTGGGACCAGATTAAGTCATGTATTGACCAGTGAGCAAGTATACGCCA790.15714825645004077No Hit
AGGTTTGGGCCTGCTTAAGTGGAGAAATAGTAGACTTGAACCAATTGAAAAA780.1551590380139643No Hit
TACGGCGGGAGCTCTCAGGCACAAATCAACATAACCACTATTCTAAAAGCTA780.1551590380139643No Hit
AGAGTTGGGACGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTC770.15316981957788786No Hit
ACGTTTGGGATTCATATTGTTTCTGGAAGAGGAAGAATTGGTTAAACAATAG760.15118060114181137No Hit
GGTCATGGGAGAGGTTAGAGATTTAAAACTCTAGTAACTTATGTATTATAAT760.15118060114181137No Hit
GTCATAGGGGTTTCAAGAGAAGAATTTGTTTACACATAAAGACAGATGCTTA750.14919138270573493No Hit
ATTTGAGGGAAGGTGGGCTTGGTCCAACAGGTTCCCAGAGGGTACATACTCC750.14919138270573493No Hit
TGCAGAGGGTGACACAACCACTTTGGAAAAATATGTGGCAGGTTCTTAAAGT750.14919138270573493No Hit
TTAAGGGGAAGCAGCAAAGCATTTAAGAGGTGGCCTGGCTGATTCTGAAAGC750.14919138270573493No Hit
CGTTTGGGGAGGTTTAAGAAAAAATTTTCTTGTCTGAAAACATAAACATCTT750.14919138270573493No Hit
ATAGAGGGGGTTACATCCACCAACAAAACACTAGGATTCCAATTTTTCCACA750.14919138270573493No Hit
AGCAAGGGGATCAGTAAAGGTATTGCTGCTGGAATGAAGAAAATGGGATACG750.14919138270573493No Hit
AGATCAGGGAAAGAGAATATCATGAAAATCAGGAAGGATTACACCATTGAAG740.14720216426965846No Hit
AAGGCTGGGAAGGCAGAATGTTCCCTGACACCAGTGTGTGGATTTTTAACAT730.145212945833582No Hit
ATCTTAGGGAGGAGGCTGAGGTGGGAGAATTGCTTGAACCTGGGAGGCGGAG730.145212945833582No Hit
CAAAAGGGGAAGTTTCTTACTACAGGTAAAAGCTATGTGCAAGAACCTCAAA730.145212945833582No Hit
TTAGAGGGAAACAATCTTTGAAGAATTTCTGAAACTGTTTACAAGGAATTTT730.145212945833582No Hit
AAGAGAGGGATGGCTGGTTGTGGTGGCTCATGCCTGTAATCCCCGCGCTTTG720.14322372739750552No Hit
AGTCACGGGATGTAAAAACCTGGATGTTAACCTCCAAGACATTGTTCACGTC720.14322372739750552No Hit
GCAAATGGGGATTACTCCTGCCATCATGACCCTTGGCCATAATATGATTTAT720.14322372739750552No Hit
TAATGTGGGGGATCTATAAATATGCTAAAGCCATTTCTATCCTTTATTTTTA720.14322372739750552No Hit
ACATTAGGGTTGCGGGGGCAGATTAAAAAATTAGAAGGAAATTTCAGTGGAT710.14123450896142906No Hit
TTTATAGGGAGTGTTAAATATGTGTGCATAAAGTCTTAAATGAAAAGATGTG700.1392452905253526No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTC700.1392452905253526No Hit
CACATCGGGGAGGGCTCAGCTCGCATGTGGAAGACTCTCACCTTCTTCGTCG700.1392452905253526No Hit
CTAAATGGGATGAAAATTTTCTGGTTTGCACCATGAATTTGTCAAATGGATA690.13725607208927612No Hit
CACGACGGGGACGCTCTTCCGATCTCTTTAGGGGATCTGTTCTCCAGTTGAA690.13725607208927612No Hit
CTAAGCGGGAATGTTATCTGGATCCCTATCTGTGAGAGTTGCTCTTCTCTCT680.13526685365319965No Hit
AACCTTGGGCTGGTGCTGCTTCATAACAAAAATGAAAAGCTGCATGCATCTA670.13327763521712319No Hit
TAGTACGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT670.13327763521712319No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTAT670.13327763521712319No Hit
CATTGCGGGAATATTCTATTTGGTATTATATTATTTGATGTTTGCTGTTCTT660.13128841678104672No Hit
GCAGAGGGGATTTGTAGATTGCAAGTTTTGTTTAAAATAACTTTATTGAGTT660.13128841678104672No Hit
CGATCCGGGTAGTTGGGTAATTCCAAGTGCTGATAGTACTATTCATCTTTTT660.13128841678104672No Hit
TTTACTGGGAGATATATTTTAAACGTGCCTTAATAGCTCAAGCTAGACTTCT660.13128841678104672No Hit
TGAACTGGAAGAGGTATGGGAAAAATGGAACTAGGATGGTGGATAAAGGGCA660.13128841678104672No Hit
TATGGAGGGGTCATCACCATGTAAGTCCCAAGATCAGTCCCGTAATATATTG660.13128841678104672No Hit
CGGTATGGGTGTGACACCTTTATATGAATTATTTTCCCAATTTTTGCTCTAT650.12929919834497025No Hit
GTCAAAGGGGCCAGGACATGTTTTCTTAAAGACAATTTAAGAGTCAGGAAGT650.12929919834497025No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC650.12929919834497025No Hit
GCCTGTGGGGGAGGGGAAGATAGTTTTGTTTTTATTATTAATTTCTAGCTGT650.12929919834497025No Hit
CTCTGTGGGATGGATTTAGAAAAAAAGATCTGTGATGAAAACATTCATTGAT650.12929919834497025No Hit
TTAGCTGGGATGACATAGAAATGGCTATGTAAACTATAATTATTTTGCTAAG650.12929919834497025No Hit
TTTGTCGGGGTTAGTGACTGATAAGGATGCCTTTGTTTTGTACATGAACCAA640.1273099799088938No Hit
TCATCCGGGGAATCACAGAATTTGTCCTTTTGTGACTGGCTAATTTCTTTTC640.1273099799088938No Hit
GAACGTGGGATACCAATGAGAGCCTCACACATTCAAGGATGTACTAAGCACT630.12532076147281732No Hit
CTGTCCGGGACTGTCTACTAGATCACTACCATTTGCTTCTTAAGTCCTCCGC630.12532076147281732No Hit
CCCTTGGGGAACTTTGAAGTGTTTGTACAGTAATGATCTTTGCTGACTGATT620.12333154303674086No Hit
ACTAGGGTGCCCCGCGGCCCTGCTGCTGGCCCCGGGGATCTGAGTCGTACCC620.12333154303674086No Hit
CATATTGGGTACCTTGATTCGTATCCTAATGTCTGATGTTATTGGTAGTTCT610.12134232460066441No Hit
TTAGTAGGGCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATC610.12134232460066441No Hit
GAGCCGGGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCG600.11935310616458794No Hit
AACGCAGGGGAAAAGATCAAACGGTGAAGTGAAGAAATGATATGAAATGATG600.11935310616458794No Hit
AAGTGGGAGAGGTTTGGTAGTAACAACACTTCTGGATCTTAAGGCTTCATAA600.11935310616458794No Hit
GTCGATGGGTGCTGCAATATAGATCAAAGATCTTGTGGCATAGGGGTAGGGG590.11736388772851146No Hit
CTGCTAGGGATGTGATAAATCACATTTATTGATTTGAATATGTTGAACCAAC590.11736388772851146No Hit
ACATGAGGGATGGCCCTTTACAATTTTTATTGCATAGTGCAATATAATAGCA590.11736388772851146No Hit
CCGATGGGGGTCACAATTCCTCAATCTGAAACAATGAGTTAAATATAATAAC580.115374669292435No Hit
TACTCTGGGATAAAAAAGAATGAAATAATGTCTTTTGCAGCAACTTGGATGA580.115374669292435No Hit
CCAAAAGGGAGAATAATTGATTTTTATCATCTTTCTTAGTATACCTTTCACG580.115374669292435No Hit
GCATAAGGGATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATAT580.115374669292435No Hit
TTGTGTGGGTATCCATTGACTTCTAATCTTCTGCTAATGGTGATGTGAATTC570.11338545085635854No Hit
GTTTTGGGGGTTTTCTGGGAGTCAGGCGAGTGTCTGTAGTCTCTGCTCGGGG570.11338545085635854No Hit
CTTTGGGGGGAAGTTTTTGCAATTCTAGAACTTGGATTTTTTTTTAACAAAA560.11139623242028207No Hit
TACAGGGGTGGTGTTGGTCAGGAAAACTAGATTGATGGGCTATCTTGCCGTG560.11139623242028207No Hit
GAGTCTGGGACTCACTGCACATCATAAATCTCCATCATCTGTCTTAAAGTTT550.1094070139842056No Hit
TATTTAGGGGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCG540.10741779554812915No Hit
GAAGGTGGGTGCATATAAAAACTATAAATGCCGAGTGGTAGAAACTCCTCTT540.10741779554812915No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC530.10542857711205267No Hit
ATACTGGGTCCACCTCCAGTGCACTTATAAACTCCTACACAGTATATAAGAT530.10542857711205267No Hit
GTGGTTGGGCTCTTGTAAGAAATGTATTAGGCCAGGTGAGGTGGTTCATGCC530.10542857711205267No Hit
TGCCATGGGGACTTGAGAATATTCTGCAGCTATAAATTTTGAACCATTGATG520.1034393586759762No Hit
GCAGTTGGGCGATCTCTGGAGGGGTAACTACTACCGCATTCCTACTACTCAA520.1034393586759762No Hit
GTAGAGGGGAATGCCCCTGGTGATCTGGGTCTATAGCACAGACACTAACTTT520.1034393586759762No Hit
GCGCCCGGCTATTTTTTTGTATTTTTAGTAGAGATGGGGCTTCACCATCTTG520.1034393586759762No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATG520.1034393586759762No Hit
GTAACTGGGAATGTTGGCATATATTATACTTTTCAAAAGCAGTTTCCACTAA510.10145014023989975No Hit
GTAACAGGGGATCTATTCTAACTCAGTTCACAGTTGAGAAAATTAAAGCCCG510.10145014023989975No Hit
CATACTGGGCTTTGTCTCTTTTTACAGATTTTGTCTTGAAATATATTTTATC510.10145014023989975No Hit
AGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCATTCCAGCCTTGGCAA510.10145014023989975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)