FastQCFastQC Report
Sat 31 Dec 2022
SRR5849078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50118
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15953.1824893251925452No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC15653.122630591803344No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3330.6644319406201366No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2030.4050440959335967No Hit
CATCAAGGGTCACAACTGAAAGTATGAACTATTGAAAAATTAATCATGGCTG1820.3631429825611557No Hit
TTCAAGGGGATAGAAAATGTCTGACTGTTTGCAAAATAATATTCTGTTTTAA1780.3551618181092621No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1730.34518536254439525No Hit
CAATAGGGGATTCACATTTTTGCCCATTTTTTAGTTGGGTTGTCTTTTTTTG1630.3252324514146614No Hit
GACCAGGGGTACAGAGGGGGCTGCAGTGTACCCTCACGAGGGACAGCCAGGA1610.32124186918871467No Hit
TGGACTGGGGTTAGAGTAAATCTATTGTGTAATTTTACATGGCATTTATATT1600.31924657807574125No Hit
ATTAACGGGCCGGCGAATCAACATGGTAGAAGAGCTTGAGAAAGCCCTGGGG1580.31525599584979447No Hit
CGCTGCGGGACGGTCTCTTTGGGTAGATATGTAGCTGCACAAGCAAATATAA1570.3132607047368211No Hit
TGCCGGGGGTTTGTTACTTGGACTAACAAGGCAGTAGATTTGCCTGTGTGGA1540.30727483139790096No Hit
AGTACTGGGAGAGGAATTAATTAAGTGGATATATACTATATTCATGGATAGG1510.3012889580589808No Hit
GCATATGGGTAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAA1490.29729837583303403No Hit
TCATTTGGGGCCAGATTTTCCTGCTTTAGTACTTGGAGAAAGTATAGCAGCA1460.2913125024941139No Hit
TAACTCGGGCACGGGGACCTGATCCGGGGCACGGTGCTCACCTACCAGTGCG1400.2793407558162736No Hit
ACCCGGGAGCATTTATTCTTGTGGATTTAATATGAATTTTAAAAACCACTTG1390.27734546470330024No Hit
TTGAGAGGGGCTCCGGCCCAGCATGGCGACCCCGACCCAGACCCCCACAAAG1380.2753501735903268No Hit
AATACCGGGAGGCGCTAAGAGGCACGCTGAGTAAACTGATTTCCCACCGGGG1380.2753501735903268No Hit
AAAGTAGGGCACATTCACTGAATTCACTGAGATTATTCCTATAATTTTAAAG1320.26337842691248653No Hit
ACACGGGATTTTGAGCGCTAATAAGCAAAAGAGCATGCAGTGCTGTTGAATA1280.255397262460593No Hit
GTGCTGGGATTACAGGCGTGAGCCACCGCACCCAGCCATGCCTGGAATATTT1230.24542080689572607No Hit
CTCTCTGGGGCTCTATAGAAATGAAAATGCAGCATGAAGTTGACATTGTGGA1220.24342551578275268No Hit
AATTGTGGGAGTCACAGTGGTGATAACATATGCTTTCAGATTTACGCAGCAT1220.24342551578275268No Hit
ATTGGTGGGATTCATACTTTTTTAACAAGCTTTTGTTATATATTTTCTAATC1220.24342551578275268No Hit
TTATGTGGGGGCCGCTTCTCCCTGCCCCGCAGCGCGCAGGGACCATGTCGGC1210.24143022466977934No Hit
TTGTCGGGGAACTCAAGGCACTGATTTCTGCATCTTCAGCCTCCTCATTTCA1200.23943493355680595No Hit
TACTCCGGGGTCAGTATAAAAGTGCCAAACCTGCCCTCTTTTAAATAAAAAT1190.23743964244383256No Hit
TAGAAGGGATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATC1180.23544435133085917No Hit
ACATTTGGGGAAGGGAAATATGTTTTTAGAAACCCTTATTAAATACTACAAT1180.23544435133085917No Hit
TTGCTTGGGCAAGGCACACATTCTACAGCAAGTCATAGAAAGTTTAATCCTT1150.229458477991939No Hit
GCATTGGGATAATTTTAGACTTACAGAAGAGTTGTAAAAGTAGTAGAGTTCT1140.22746318687896566No Hit
CTCGTAGGGTAGCCGGCTGGAGTACGGGGCCTTCGGGAGCCGCGCGCCTTCT1130.22546789576599227No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1090.2174867313140987No Hit
GCACTTGGGAAGAAAACACTGAATACGTAACTTTAAAGGATCCCTGAAGAAA1060.2115008579751786No Hit
ACTCATGGGTATGGCTTTATTTTTGGAGGCTTAAAAAATTAAAAACTCTAGA1050.2095055668622052No Hit
CATGTGGGGTAATCACAAACCCCAAAACGGGAGAGAGCAGTGAAAACATGCA1050.2095055668622052No Hit
TGAAAAGGGTGGATATATATAGTATTGGGTGATGGTGCAGAATACAAAAGTT1050.2095055668622052No Hit
AGTTAGGGGCCTTAGAGCTTGAGCTGCCAGAGTCCCCTGTGAATCAAGATTT1050.2095055668622052No Hit
CTAATTGGGATTTAAAACAGTGGCATCTGATTTATAAGGTGGCATTTGGGAT1020.20351969352328503No Hit
GTTCTGGGGAACTCCTTTGTGCCTCAAGATCCTTTTTTTCTGTTCCTTTTCA1020.20351969352328503No Hit
ACTAGCGGGAGATGTGTCAGAAACATAGTCAGGATGTGCCATTAACTTTGAT1010.20152440241031164No Hit
CTTGGGTTGGGTCGAGCCAAATCGCATGGCTTTCAGATCAGGTATTCTGCAC1000.1995291112973383No Hit
TAAAGCGGGGTACACATATGTCACCACGAGTAGTGAATCGCATGAGCCGTTT1000.1995291112973383No Hit
GTATGTGGGAACACCCTAGTTTTCTGAACTGCAGCTGCATGGGTAATTATCT980.19553852907139152No Hit
TGATAAGGGTACGGAGGAATGATTGTTTAAACCATTGCCACTGGTGATCAAC950.18955265573247138No Hit
GGTGAGGGTAAGAGTCTGGAGGACACCTGTATATTTATACTGTCCATTTCGG950.18955265573247138No Hit
TCGATAGGGTGGTGCAAAAGTGATCCGCATTCAGTAGAAACTGTTCCTCAAG950.18955265573247138No Hit
TGAACTGGAAGAGGTATGGGAAAAATGGAACTAGGATGGTGGATAAAGGGCA950.18955265573247138No Hit
GCCCGAGGGGATACACTACCTCTTACGTGTTGCCTCTTTGTGTTGCTTGGTG940.18755736461949798No Hit
TTAGAGGGAAACAATCTTTGAAGAATTTCTGAAACTGTTTACAAGGAATTTT940.18755736461949798No Hit
TTTGTCGGGGTTAGTGACTGATAAGGATGCCTTTGTTTTGTACATGAACCAA940.18755736461949798No Hit
TACGGCGGGAGCTCTCAGGCACAAATCAACATAACCACTATTCTAAAAGCTA930.1855620735065246No Hit
GAAAAAGGGTGGATTAGTACCATGGTCTGACTTGAAAGTGTTTTCGGTAAGT930.1855620735065246No Hit
GCTCGTGGGATAGGAATTCCTGGGACCTCTTTGACTTTTAACTTCTTATACA920.18356678239355123No Hit
AGCAAGGGGATCAGTAAAGGTATTGCTGCTGGAATGAAGAAAATGGGATACG920.18356678239355123No Hit
CTCCCCGGGCATTTCCAGCTCTTCACTTTTTGGCACTGGATGCATCTCAGCA910.18157149128057784No Hit
CACATCGGGGAGGGCTCAGCTCGCATGTGGAAGACTCTCACCTTCTTCGTCG910.18157149128057784No Hit
TGTCGTGGGAGTAGTGTGGAAACTTAGAGAACTTATTGTATGGTGCCTTGCA910.18157149128057784No Hit
ACAATTGGGATTTTTTCCCCTGGACTATACTTAGAAATACTACATCAATTGT900.17957620016760445No Hit
ATAGAGGGGGTTACATCCACCAACAAAACACTAGGATTCCAATTTTTCCACA890.17758090905463106No Hit
GCCATGGGCGCTTCCGATCTTAGCTAGGGAATCCAGATGTAAACAACCAGAT880.1755856179416577No Hit
TATGAAGGGGGAGATGATACAGGATGGCCACCAGGTGTCTTTGTTAAGCGGG870.1735903268286843No Hit
CTAAATGGGATGAAAATTTTCTGGTTTGCACCATGAATTTGTCAAATGGATA870.1735903268286843No Hit
GCCATGGGCGCTCTTTCCGATCTCATTAGGGGTAAAGAAGTGAGGGCTGTGG870.1735903268286843No Hit
AGAGTTGGGACGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTC870.1735903268286843No Hit
ATCTTAGGGAGGAGGCTGAGGTGGGAGAATTGCTTGAACCTGGGAGGCGGAG860.17159503571571091No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT860.17159503571571091No Hit
TTTACTGGGAGATATATTTTAAACGTGCCTTAATAGCTCAAGCTAGACTTCT860.17159503571571091No Hit
CTAGGCGGGCCATTTACCTAATTTTACTAGTGGATTTTATGCCTTCAAATGT860.17159503571571091No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC850.16959974460273755No Hit
GTCATAGGGGTTTCAAGAGAAGAATTTGTTTACACATAAAGACAGATGCTTA850.16959974460273755No Hit
GAGCCGGGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCG850.16959974460273755No Hit
CAAACTGGGACTTATTTTCTGAATAATTATATATGAATGCCCAAAAATAAAC840.16760445348976416No Hit
ATTGGGGGGAGCGCAGGCGCGGTGGCGCGAGTTGGACTGTGAAGAAACATGG840.16760445348976416No Hit
GCGAACTAGGCAGAAAGTAATTGTCACTCATTCACACACAGAACTTCCATCA840.16760445348976416No Hit
CGATCCGGGTAGTTGGGTAATTCCAAGTGCTGATAGTACTATTCATCTTTTT840.16760445348976416No Hit
AAGTGGGGTGATGAGAGTGTCTATGGGAAATGAGTGGTTGGAAGCCCAGCAG830.16560916237679077No Hit
TCTGGGGAGCATGGACGATCAGGAAGAAATGGAGGAAGAAGCAGAAATGGAA830.16560916237679077No Hit
TTAGGAGGGCCAGTTAACATTTAGGGAGTTATCTGTTTTCATCTTGAGGTGG820.16361387126381738No Hit
CTGGTTGCGGGATGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGG820.16361387126381738No Hit
ATTTGAGGGAAGGTGGGCTTGGTCCAACAGGTTCCCAGAGGGTACATACTCC820.16361387126381738No Hit
TTTATAGGGAGTGTTAAATATGTGTGCATAAAGTCTTAAATGAAAAGATGTG820.16361387126381738No Hit
CAGACCTGGGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGAC820.16361387126381738No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT810.16161858015084402No Hit
ACGTTTGGGATTCATATTGTTTCTGGAAGAGGAAGAATTGGTTAAACAATAG810.16161858015084402No Hit
ACATTAGGGTTGCGGGGGCAGATTAAAAAATTAGAAGGAAATTTCAGTGGAT810.16161858015084402No Hit
CTGTCCGGGACTGTCTACTAGATCACTACCATTTGCTTCTTAAGTCCTCCGC810.16161858015084402No Hit
GCGCTGGGAGATCTGTTTACTAGCCAATGAGCTGTTTGATGTCTGAGGGAAA800.15962328903787062No Hit
TATGGAGGGGTCATCACCATGTAAGTCCCAAGATCAGTCCCGTAATATATTG800.15962328903787062No Hit
AAGGCTGGGAAGGCAGAATGTTCCCTGACACCAGTGTGTGGATTTTTAACAT790.15762799792489723No Hit
GAAGGTGGGTGCATATAAAAACTATAAATGCCGAGTGGTAGAAACTCCTCTT790.15762799792489723No Hit
AACTTTGGGACCAGATTAAGTCATGTATTGACCAGTGAGCAAGTATACGCCA780.15563270681192387No Hit
TAGTACGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT770.15363741569895048No Hit
ATAGCCGGGAGACAATCCACAATGTATACATATAGCAAAACATCATGTCGTA770.15363741569895048No Hit
TCGTCAGGGATTACTTGCACCAGAAATGATTTCAAACACTTTAGGAACTGTG760.1516421245859771No Hit
TAATGTGGGGGATCTATAAATATGCTAAAGCCATTTCTATCCTTTATTTTTA750.1496468334730037No Hit
AAGAGAGGGATGGCTGGTTGTGGTGGCTCATGCCTGTAATCCCCGCGCTTTG730.14565625124705694No Hit
GTTAAAGGGATGAGTACGGTGTGTTTCTGAGAGAGTGTGATGTTCACCTCTG730.14565625124705694No Hit
AGATCAGGGAAAGAGAATATCATGAAAATCAGGAAGGATTACACCATTGAAG730.14565625124705694No Hit
GCAGAGGGGATTTGTAGATTGCAAGTTTTGTTTAAAATAACTTTATTGAGTT730.14565625124705694No Hit
GGTCATGGGAGAGGTTAGAGATTTAAAACTCTAGTAACTTATGTATTATAAT730.14565625124705694No Hit
ACACAGGGACAATAATATTCAACAGAACTTAACCTGGACCTTATGTTGTAAT710.1416656690211102No Hit
GCATAAGGGATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATAT710.1416656690211102No Hit
CGTTTGGGGAGGTTTAAGAAAAAATTTTCTTGTCTGAAAACATAAACATCTT700.1396703779081368No Hit
AGTCACGGGATGTAAAAACCTGGATGTTAACCTCCAAGACATTGTTCACGTC700.1396703779081368No Hit
AAGTACGGGATTCCCATTATCTTTGACCAGTATTAATTTTTGAGATCTTACT700.1396703779081368No Hit
GTCGATGGGTGCTGCAATATAGATCAAAGATCTTGTGGCATAGGGGTAGGGG690.1376750867951634No Hit
TTGTGTGGGTATCCATTGACTTCTAATCTTCTGCTAATGGTGATGTGAATTC690.1376750867951634No Hit
TAAGCGGGGAATAAGCAAATATTGTCTTCAAAAGAATGCACAAGAACCACAA690.1376750867951634No Hit
TAATCAGGGAGCTGAATTCACATATCTGATGAGATTAGACAGTTTATCAAAA690.1376750867951634No Hit
CAAAAGGGGAAGTTTCTTACTACAGGTAAAAGCTATGTGCAAGAACCTCAAA690.1376750867951634No Hit
AAGTGGGAGAGGTTTGGTAGTAACAACACTTCTGGATCTTAAGGCTTCATAA690.1376750867951634No Hit
CTCTGTGGGATGGATTTAGAAAAAAAGATCTGTGATGAAAACATTCATTGAT670.13368450456921666No Hit
ACTAGGGTGCCCCGCGGCCCTGCTGCTGGCCCCGGGGATCTGAGTCGTACCC670.13368450456921666No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTAT670.13368450456921666No Hit
GTGGTTGGGCTCTTGTAAGAAATGTATTAGGCCAGGTGAGGTGGTTCATGCC670.13368450456921666No Hit
CCGATGGGGGTCACAATTCCTCAATCTGAAACAATGAGTTAAATATAATAAC650.12969392234326987No Hit
AACTTAGGGATCTCTTTTCATTCAACGATGTAATGAAGTAGGAATTATACTT640.1276986312302965No Hit
TCATCCGGGGAATCACAGAATTTGTCCTTTTGTGACTGGCTAATTTCTTTTC640.1276986312302965No Hit
AACCTTGGGCTGGTGCTGCTTCATAACAAAAATGAAAAGCTGCATGCATCTA630.12570334011732312No Hit
CTTTGGGGGGAAGTTTTTGCAATTCTAGAACTTGGATTTTTTTTTAACAAAA630.12570334011732312No Hit
GTAACTGGGAATGTTGGCATATATTATACTTTTCAAAAGCAGTTTCCACTAA630.12570334011732312No Hit
TTAAGGGGAAGCAGCAAAGCATTTAAGAGGTGGCCTGGCTGATTCTGAAAGC630.12570334011732312No Hit
CACGACGGGGACGCTCTTCCGATCTCTTTAGGGGATCTGTTCTCCAGTTGAA630.12570334011732312No Hit
CTGCTAGGGATGTGATAAATCACATTTATTGATTTGAATATGTTGAACCAAC620.12370804900434973No Hit
TCAATAGGGGTGACGTGTTGTAGTTTTTAGATGTTTGTAAAATGTTTAAAAA620.12370804900434973No Hit
GCGTTGGGGATATATTTATAAGTTTAATGATATTCTCAACAAAATTCCAATG620.12370804900434973No Hit
GTTTTGGGGGTTTTCTGGGAGTCAGGCGAGTGTCTGTAGTCTCTGCTCGGGG620.12370804900434973No Hit
GATACGGGGCCCGAGGCCCTGCGCTCTCGGCCGGGCTCCCCAGCCCTCTGCT620.12370804900434973No Hit
CGGTATGGGTGTGACACCTTTATATGAATTATTTTCCCAATTTTTGCTCTAT610.12171275789137634No Hit
AACGCAGGGGAAAAGATCAAACGGTGAAGTGAAGAAATGATATGAAATGATG610.12171275789137634No Hit
TTAGCTGGGATGACATAGAAATGGCTATGTAAACTATAATTATTTTGCTAAG610.12171275789137634No Hit
GCAAATGGGGATTACTCCTGCCATCATGACCCTTGGCCATAATATGATTTAT600.11971746677840298No Hit
CAAGTAGGGGGCTTTTAAAAAGGCTATCTTAGGCCTGGTGCAGTGGCTCACA590.11772217566542958No Hit
TATTTAGGGGAGATGAGTTTCTGAGGATCAGCACTGCCAGTGGAGATGGGCG580.1157268845524562No Hit
TGCAGAGGGTGACACAACCACTTTGGAAAAATATGTGGCAGGTTCTTAAAGT580.1157268845524562No Hit
CCAAAAGGGAGAATAATTGATTTTTATCATCTTTCTTAGTATACCTTTCACG580.1157268845524562No Hit
TTGCCGCGGGTACTGATTAGACTTTGAGCCAAGGGGAAAATACTAAATTCTT580.1157268845524562No Hit
ACATGAGGGATGGCCCTTTACAATTTTTATTGCATAGTGCAATATAATAGCA570.11373159343948283No Hit
CGACGCGGGTATCCTCAAGTTGTGTGCTATTCGTAAGTTCTGTGCAGTTTGG570.11373159343948283No Hit
CATATTGGGTACCTTGATTCGTATCCTAATGTCTGATGTTATTGGTAGTTCT560.11173630232650944No Hit
GTCAAAGGGGCCAGGACATGTTTTCTTAAAGACAATTTAAGAGTCAGGAAGT560.11173630232650944No Hit
TTGGACTGGGATTCAAATTCAATAAGTGATGCTGAAACAACAGGTTATCTAT560.11173630232650944No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTC560.11173630232650944No Hit
TTAGTAGGGCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATC560.11173630232650944No Hit
CACGATGGGTGACTTCCATTGCAGTTCATCAATGGTGCTAAGGAAATTTGCT560.11173630232650944No Hit
AATACTGGGAATGGAAGGTTTTTTGTGATGTATGAAACTTGTGTTTTTTATA550.10974101121353605No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACTGTCTCTTATACAC550.10974101121353605No Hit
AGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCATTCCAGCCTTGGCAA550.10974101121353605No Hit
AGGTTTGGGCCTGCTTAAGTGGAGAAATAGTAGACTTGAACCAATTGAAAAA540.10774572010056266No Hit
GTAGAGGGGAATGCCCCTGGTGATCTGGGTCTATAGCACAGACACTAACTTT540.10774572010056266No Hit
AGTTTAGGGACTGTGTTACAATAATGGCAAGTAACATGTTAAGAAGGTTTAC530.1057504289875893No Hit
TACAGGGGTGGTGTTGGTCAGGAAAACTAGATTGATGGGCTATCTTGCCGTG530.1057504289875893No Hit
GACCCGGGGGACTCTGAGTAACAGATGTTCTCAGCCTTGTGTTTCCTGTCTG520.1037551378746159No Hit
GCTAAAGGGGAACAATTGTGTTTTCGTCACAGGAGTCTTTGTAATGCTTGTA520.1037551378746159No Hit
GACTTTGGGAAACCAAGCATTGAATAATGGCTGGATAACTGCCGAAGTAAGC520.1037551378746159No Hit
GTCATCGGGAATGTTTATATATATATTTTTAAATTACTGAATTTCAAAATAT520.1037551378746159No Hit
AACAGAGGGGGAACAACAACAAAAAATCCTATGAAGTTTTTATAGAAAAGTG520.1037551378746159No Hit
GCGATTGGGTTTCAAATCAAATTTGGATGCCAACTATGTGGAACAGAAGTGT510.10175984676164251No Hit
CATACTGGGCTTTGTCTCTTTTTACAGATTTTGTCTTGAAATATATTTTATC510.10175984676164251No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATG510.10175984676164251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)