FastQCFastQC Report
Sat 31 Dec 2022
SRR5849074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112400
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC23812.1183274021352316No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT22461.998220640569395No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC2950.2624555160142349No Hit
TCTTTGGGGGATCTTGACTTGGGGTAAGAATGTAAGGAGGGATAAGAACGAG2080.18505338078291814No Hit
GTCAATGGGGATGACATCCAATTATTATTTTTTTCTTTTATGTTTTTTGTGT2000.1779359430604982No Hit
CGACTCACTGCAACCTCTTCCTCCCAGGTTCAAGCATTTCTCCTGCCTCAGC1980.17615658362989323No Hit
GCAGGTGGGTCTGCAAGAGGGAAGACCTATAGATGTAAATATGGATGTAGAT1870.16637010676156583No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1790.1592526690391459No Hit
TTAGGAGGGTAATAAAAAGAGCTGCTGCTGGACATTTATCAAAGATAGGCAC1680.1494661921708185No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1560.1387900355871886No Hit
GTGTGCGGGTAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG1540.13701067615658363No Hit
AAAGGTGGGGAGAGCAAGCACTCTAGATGAAATAGCAGGTTATAGTAAACTG1530.13612099644128114No Hit
GTACAGGGGACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGG1520.13523131672597866No Hit
TGTTTAGGGGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT1510.13434163701067614No Hit
CGTTCTGGGATTGGCAGTGCCTGCTCTTGTCAATACTCCTGCCCCAAAATGC1490.13256227758007116No Hit
AGACTCGGGACCTGGAAGAGAGCCTGCCCTGCAGAAGACCCTGATTCAATTA1480.1316725978647687No Hit
TTCACTGGGATTATAGGCATGAGCCACTGCATCAGGCTAAAGAGCACATATT1480.1316725978647687No Hit
TCCGCACAGGGGACTGCCTGCCGCGTGCGGAGCCGGAGCCCGAGCCTGAGTG1480.1316725978647687No Hit
GATGCAGGGATCCTGGAGTTGGGTTGCTAACCTTAATTTCTATGATGTTTTC1450.1290035587188612No Hit
GGTAACGGGCGATCTCGACCTGGGGACTCAGATAGCTCAAGTGATTCTCAGT1440.12811387900355872No Hit
CATGCAGGGGGGCCCTCAGGGCTGGGGAGCTCAGTTCCCATTGCTTGTGCTC1430.12722419928825623No Hit
GCCAAAGGGATGTGGGAGAGCAGATTGCTGTTCCCCGCAATGATGAATGGGC1430.12722419928825623No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC1380.12277580071174378No Hit
TTGTTTGGGATGGATCTCCCGAAATTGGCCAGTGACGACCGCCCAAGCCCTC1380.12277580071174378No Hit
TTTTATGGGGTTTTCCCAATTCTACACATGGCAAAATGGAGACCCAGAGTCA1370.12188612099644128No Hit
AAGCAAGGGATTCCTCCACGGAAGGGAAGACAAGTATCAACACCACTGGCAT1370.12188612099644128No Hit
GCGACAGGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA1360.12099644128113879No Hit
TCAGCTGGGAACACATGATGTCATCAATAACCTACTGAAATTTATTCTACTT1350.1201067615658363No Hit
TAGATGGGGGTTTGTTATTGGGGGTGTGGCCCAGGGCCCCAGTCAAGCCGTG1350.1201067615658363No Hit
GGCAAAGGGCGGCAAGGCCAAGCTGCGCAGCGTGAAGGAGCGAAAGCTGGAG1350.1201067615658363No Hit
AGTTATGGGATTTCCAAGTTACTCTAAAGAAACCCAGTATAAAATAACTAGT1350.1201067615658363No Hit
ACCGTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1340.11921708185053381No Hit
TCAGTTGGGACAACTGTAGCAATCATGCCATTTGGCTAAATGTACAGAGTAC1330.11832740213523131No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1310.11654804270462633No Hit
GAAAGTGGGAAATCAGAGAAGCCAGAAAGCTGCGATAATGTGAAGGTTGTTG1300.11565836298932383No Hit
AATGTCGGGATGAAGGAAAAAAAGCAGATCTGTTCAAATGCATGCTTATGTC1290.11476868327402136No Hit
CTACTAGGGACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACC1290.11476868327402136No Hit
ACCTGAGGGTGGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGCGC1280.11387900355871886No Hit
TATTAAGGGATAAGTGGGAGCTAAACAGTGAGGACACATGGACACGTAGACA1280.11387900355871886No Hit
GAAACCGGGGGACAGGAATATGAAATTAGAAGATTAGAAGACAGGTGAGGAA1270.11298932384341638No Hit
GTAAATGGGAAGATTTAATCATTTGCTAATGGAAATCTTACCACCTTTCATT1260.11209964412811388No Hit
TGCATCGGGTGAGAATCACTAGTATAATCCAGTATGCTCTTCCAGCATCACA1260.11209964412811388No Hit
CACAATGGGGAAGAAAAAGTGTAACACTAAGGTGAACTTGCTTCCCTTTTCA1250.1112099644128114No Hit
TGCGCTAGGGGGGAGACCATCAGCTCATCCAGGAGGCCCTTGTCTTTGACAA1250.1112099644128114No Hit
ATCACTGGGACCCCCTCTTCGTTATCTCCGTAACCCCAGTCCAAATAAACAA1250.1112099644128114No Hit
TGAACCGGGAACACAGTGGTAGGTATTTGTGTATATGAACGTAGACAAGGGA1250.1112099644128114No Hit
ATGTATGGGTATCCTTTGCCTAAAGAAATCTCTTTACAAAATCTAAACTATG1240.1103202846975089No Hit
TCTAAAGGGGTGCTTTCAAATAACTAACACGAACTTCCAGAAAATTAACATT1240.1103202846975089No Hit
CAAAAAGGGGGGCCTCGGTCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCT1240.1103202846975089No Hit
ACTTTAGGGACCCCAGGCCCAAGCGCCTTGCTGTCACGCCCAACCTCTTTGG1230.1094306049822064No Hit
GCCATAGGGAAGAAATGCTATGTGTACTGTGATGTAAAAATAAGACATTAAC1220.10854092526690393No Hit
TGTACCGGGGTCACAGCTGCTGCCCCAGCTCTGGAACTCCCAGACGTCCCAG1220.10854092526690393No Hit
CAATTGGGGGATAAATTAACAAAAATAAAACCCTCTGGTTACTTCAGATAAA1210.10765124555160142No Hit
ATGTCAGGGAGTCTATGACTGAATTTTGCTTTTATGGCTTTTGCTCTCAAAA1210.10765124555160142No Hit
CATAACCGGGGTAAATGTGAATGGAAACTTGGAAATACTCGTTTTTATAAAC1210.10765124555160142No Hit
CATGATGGGTGGAGTTATACAACTTGGCTTGTTTACCTCCACATGCTGATGA1210.10765124555160142No Hit
CACTTAGGGACCGCTTCTATGCCGAAGCTAGCCTATTGAGAGTCATGGTGGT1190.10587188612099643No Hit
GGATAAGGGCCACAGTGAAGCACGAAGGCCTGGGGAATCCTGACTGTGGCTC1180.10498220640569396No Hit
GTATTAGGGGGGAATCCAGAGAGGGGCAGTAGTAGAGTGGGCCAGTTGGGAC1180.10498220640569396No Hit
AAAAAGGGGGTTTTTATGTAAATGGTTGGAAAAAATCAAAAGAAGAACTAGA1170.10409252669039146No Hit
CAAGCAGGGGCATTAGGTCTTGTGTTTGATGTGTTCTTGTGAATTTACTTTG1170.10409252669039146No Hit
AGCTAAGGGCGTCTCCATCAGTGTTAACACAGGAGAGATATGTTATTTTATG1170.10409252669039146No Hit
AGCAATGGGATGGCCTCAGCCAACTATCTTAGCTCCAGGTCACGGCCCCATC1170.10409252669039146No Hit
TAGTCAGGGATAGTGCTTCTGTATTTTCTACTCAAACATTCTTTGGAACGAT1160.10320284697508895No Hit
CGTATTGGGAAAGACACATGAGCCAGAGAAGCCGGCATGAGCAGAGATGCCC1160.10320284697508895No Hit
CGATGGGGGGCTTTCCTCTCGGCTGAGCCTCACGTTTGGAGCAGTTCTTGCG1160.10320284697508895No Hit
ATCATAGGGACTGCATTCATTCTGAGCCCCCTTCCTGTCCCCAGGGGAGGTG1160.10320284697508895No Hit
GTCTAAGGGGAGTGACAGATAATCAGGAGAAGAAAAGGAGATCCAGACTCAT1160.10320284697508895No Hit
GCACCTGGGATTTCCCGCACATATGATGCCACCACTCATTTTGAGACAACGT1160.10320284697508895No Hit
GATGCTGGGAAAGGCGTAAAACAACATGCCCATGTCCCCAAAGAAGGAAATT1150.10231316725978648No Hit
GTCTACGGGGATTTTCTGGGAGCTATCTTGTCTACCTTGAGGTTCCTGAACA1150.10231316725978648No Hit
TCGTTGGGGGCAGATGGCCATGGTGAAGTGTGGACAGATTGGAATAATATGT1140.10142348754448398No Hit
CTGGAGGGGGGCAATTGAAATGCAGGTGCAGTTTTCTGTTAATGTCATGCTG1140.10142348754448398No Hit
TATTTGGGACCCAAGCCAATATTTTGTGCTATGAAACCTGGAGGTATTCATC1140.10142348754448398No Hit
TATGCCGGAGGGAAACCCCCATCTCTACAAAAAATAAAAATAAAAAATCAGC1140.10142348754448398No Hit
CCTCAAGGGGCCACATAAATGTCTTCTTTTGAGAAGTGTTTGTTTATGTCCT1140.10142348754448398No Hit
CTGTTTGGGAAAGTAAGAATATATTATTAGAACTTGCCCTGTGGAGACACCT1140.10142348754448398No Hit
GCGGCAGGGATAAAGTGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGAC1140.10142348754448398No Hit
CCCAGTGGGACTTAGGTTCAGAAATATACACACACTTACTCTTTAGCCAGTT1140.10142348754448398No Hit
TACTATGGGGCTGCCAGACTTGGAAAGTCAATTAGTAGACTCATCGTTGTGG1140.10142348754448398No Hit
TTAGTTGGGAAGGACATTTTTTTTCCTCTTTTTTTTTTTTTTTTTTTTTTTT1130.1005338078291815No Hit
TTGGCTGGGTCTGCAGATGTTCTAGTTAAGCTAAAAAGTAAAAACTTTTTTA1130.1005338078291815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)