FastQCFastQC Report
Sat 31 Dec 2022
SRR5849073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111467
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC23382.0974817659038103No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT22251.996106470973472No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC2780.24940116805870796No Hit
TCTTTGGGGGATCTTGACTTGGGGTAAGAATGTAAGGAGGGATAAGAACGAG2160.19377932482259325No Hit
GTCAATGGGGATGACATCCAATTATTATTTTTTTCTTTTATGTTTTTTGTGT2120.19019081880736002No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1790.16058564418168605No Hit
TTTTATGGGGTTTTCCCAATTCTACACATGGCAAAATGGAGACCCAGAGTCA1790.16058564418168605No Hit
CGACTCACTGCAACCTCTTCCTCCCAGGTTCAAGCATTTCTCCTGCCTCAGC1680.15071725263979474No Hit
TTAGGAGGGTAATAAAAAGAGCTGCTGCTGGACATTTATCAAAGATAGGCAC1680.15071725263979474No Hit
TCAGCTGGGAACACATGATGTCATCAATAACCTACTGAAATTTATTCTACTT1670.14982012613598644No Hit
GTACAGGGGACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGG1620.14533449361694492No Hit
GACCTTGGGATCCTCATCGCTGTGGGCGCTGTCATGATGTTCGTTGGCTTCC1580.14174598760171173No Hit
CGTTCTGGGATTGGCAGTGCCTGCTCTTGTCAATACTCCTGCCCCAAAATGC1570.1408488610979034No Hit
TGCGCTAGGGGGGAGACCATCAGCTCATCCAGGAGGCCCTTGTCTTTGACAA1560.1399517345940951No Hit
ATGTATGGGTATCCTTTGCCTAAAGAAATCTCTTTACAAAATCTAAACTATG1550.1390546080902868No Hit
AGACTCGGGACCTGGAAGAGAGCCTGCCCTGCAGAAGACCCTGATTCAATTA1520.1363632285788619No Hit
GCAGGTGGGTCTGCAAGAGGGAAGACCTATAGATGTAAATATGGATGTAGAT1500.1345689755712453No Hit
GCGACAGGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA1500.1345689755712453No Hit
CAAAAAGGGGGGCCTCGGTCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCT1500.1345689755712453No Hit
TGTTTAGGGGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT1490.133671849067437No Hit
GTGTGCGGGTAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG1490.133671849067437No Hit
AAAGGTGGGGAGAGCAAGCACTCTAGATGAAATAGCAGGTTATAGTAAACTG1490.133671849067437No Hit
AAGCAAGGGATTCCTCCACGGAAGGGAAGACAAGTATCAACACCACTGGCAT1450.1300833430522038No Hit
AAAAAGGGGGTTTTTATGTAAATGGTTGGAAAAAATCAAAAGAAGAACTAGA1440.1291862165483955No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1420.12739196354077886No Hit
CATAACCGGGGTAAATGTGAATGGAAACTTGGAAATACTCGTTTTTATAAAC1420.12739196354077886No Hit
TAGATGGGGGTTTGTTATTGGGGGTGTGGCCCAGGGCCCCAGTCAAGCCGTG1410.1264948370369706No Hit
GCAAGCGGATCTTATTCTTCATGTAATCCCACCTTCTAACAAAAAATAAATA1390.12470058402935398No Hit
GGCAAAGGGCGGCAAGGCCAAGCTGCGCAGCGTGAAGGAGCGAAAGCTGGAG1390.12470058402935398No Hit
ACTTTAGGGACCCCAGGCCCAAGCGCCTTGCTGTCACGCCCAACCTCTTTGG1380.12380345752554568No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC1380.12380345752554568No Hit
TCCGCACAGGGGACTGCCTGCCGCGTGCGGAGCCGGAGCCCGAGCCTGAGTG1380.12380345752554568No Hit
TCAGTTGGGACAACTGTAGCAATCATGCCATTTGGCTAAATGTACAGAGTAC1370.12290633102173738No Hit
CCTGCCCCTCCTGCCAGGCCCTCCCTCCCGGGTGGTGGCCGGCACCTCTGCT1360.12200920451792907No Hit
CTACTAGGGACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACC1350.12111207801412077No Hit
ATCACTGGGACCCCCTCTTCGTTATCTCCGTAACCCCAGTCCAAATAAACAA1330.11931782500650416No Hit
GAGCTGGGGAGAGGGAGGCGGCTGGCCCGGCGGCAGGCTCTCAGAACCGCTA1330.11931782500650416No Hit
GTATTAGGGGGGAATCCAGAGAGGGGCAGTAGTAGAGTGGGCCAGTTGGGAC1330.11931782500650416No Hit
TCGCCAGGGAATTGTCTAAGGCATAGAGAGATTAGTAACTGTTGATACTGAT1320.11842069850269586No Hit
GATGCTGGGAAAGGCGTAAAACAACATGCCCATGTCCCCAAAGAAGGAAATT1310.11752357199888755No Hit
GTAAATGGGAAGATTTAATCATTTGCTAATGGAAATCTTACCACCTTTCATT1310.11752357199888755No Hit
CATGCAGGGGGGCCCTCAGGGCTGGGGAGCTCAGTTCCCATTGCTTGTGCTC1310.11752357199888755No Hit
TGTACCGGGGTCACAGCTGCTGCCCCAGCTCTGGAACTCCCAGACGTCCCAG1310.11752357199888755No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1290.11572931899127097No Hit
GCCAAAGGGATGTGGGAGAGCAGATTGCTGTTCCCCGCAATGATGAATGGGC1290.11572931899127097No Hit
AGTTATGGGATTTCCAAGTTACTCTAAAGAAACCCAGTATAAAATAACTAGT1280.11483219248746267No Hit
TAGTCAGGGATAGTGCTTCTGTATTTTCTACTCAAACATTCTTTGGAACGAT1270.11393506598365435No Hit
AATGTCGGGATGAAGGAAAAAAAGCAGATCTGTTCAAATGCATGCTTATGTC1270.11393506598365435No Hit
GGTAATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.11393506598365435No Hit
TTGTTTGGGATGGATCTCCCGAAATTGGCCAGTGACGACCGCCCAAGCCCTC1270.11393506598365435No Hit
TATTAAGGGATAAGTGGGAGCTAAACAGTGAGGACACATGGACACGTAGACA1260.11303793947984606No Hit
ATCATAGGGACTGCATTCATTCTGAGCCCCCTTCCTGTCCCCAGGGGAGGTG1230.11034655996842115No Hit
ATGTCAGGGAGTCTATGACTGAATTTTGCTTTTATGGCTTTTGCTCTCAAAA1230.11034655996842115No Hit
GGGCCAGGGGTCACTTTTCTGTGAGAGGTATTCATCCCTGACCTTGAAATAT1230.11034655996842115No Hit
GCACCTGGGATTTCCCGCACATATGATGCCACCACTCATTTTGAGACAACGT1220.10944943346461285No Hit
TGCATTGGGAAATTAATGCTAATTAAAGATTAATGTGTTGTAATTAGCATTA1210.10855230696080453No Hit
CAAGCAGGGGCATTAGGTCTTGTGTTTGATGTGTTCTTGTGAATTTACTTTG1200.10765518045699624No Hit
CGTATTGGGAAAGACACATGAGCCAGAGAAGCCGGCATGAGCAGAGATGCCC1190.10675805395318792No Hit
TCTATAGGGATTCCAAGAGATATACAGATTTTAAGAATAGATGCTCAGTAAT1190.10675805395318792No Hit
CCTCAAGGGGCCACATAAATGTCTTCTTTTGAGAAGTGTTTGTTTATGTCCT1180.10586092744937965No Hit
CAGTGCTGGGAGACTATTCAAAATACTTCCTCGGAGCTTGTATAGATTTCAG1180.10586092744937965No Hit
TTCACTGGGATTATAGGCATGAGCCACTGCATCAGGCTAAAGAGCACATATT1180.10586092744937965No Hit
CACAATGGGGAAGAAAAAGTGTAACACTAAGGTGAACTTGCTTCCCTTTTCA1170.10496380094557134No Hit
ACCTGAGGGTGGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGCGC1170.10496380094557134No Hit
ACAGTTGGGGGGGACTAGGTTTCAGCGGCCGCTGCGATGACCAAAATAAAGG1170.10496380094557134No Hit
CTACGCTTGGGTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATC1170.10496380094557134No Hit
GAAAGTGGGAAATCAGAGAAGCCAGAAAGCTGCGATAATGTGAAGGTTGTTG1170.10496380094557134No Hit
GTCTACGGGGATTTTCTGGGAGCTATCTTGTCTACCTTGAGGTTCCTGAACA1170.10496380094557134No Hit
CACTTAGGGACCGCTTCTATGCCGAAGCTAGCCTATTGAGAGTCATGGTGGT1160.10406667444176304No Hit
GTCTAAGGGGAGTGACAGATAATCAGGAGAAGAAAAGGAGATCCAGACTCAT1160.10406667444176304No Hit
AGACTTGGGACCCTTCTGAACAAAACATGTACTTACTCTCCGAAAGGCATCT1150.10316954793795474No Hit
TATGCCGGAGGGAAACCCCCATCTCTACAAAAAATAAAAATAAAAAATCAGC1150.10316954793795474No Hit
TACCATGGGGGGGTGTACGAAGCGGCCAAGCTGCTCAACGTGCGTTTTGCTG1150.10316954793795474No Hit
AACAGGGGAGACTCAGGGCCAAGAAATTGGTAAGGAAGACAAAATAACATGA1150.10316954793795474No Hit
ACTTCGGGTGAGGTCCTCACCTTCCTCTCAGTAACTACAGAGAAGACGTTTC1150.10316954793795474No Hit
ACGCATGGGGGGAAATTTTGATATCAGTTGAAAAGGCATTCCAAATGAAGAG1140.10227242143414643No Hit
AACAAGGGGGGAACACAACAGAGCCCTCTCGAACATGCAAAGATCCTATAGA1140.10227242143414643No Hit
TGAACCGGGAACACAGTGGTAGGTATTTGTGTATATGAACGTAGACAAGGGA1130.10137529493033813No Hit
GGTAACGGGCGATCTCGACCTGGGGACTCAGATAGCTCAAGTGATTCTCAGT1120.10047816842652982No Hit
AGAACAAGGGTGTGAGAAGGAAAAATAATAGCCAGGTTTTAAGTTTTCCATA1120.10047816842652982No Hit
GGCACTGGGACATCACACCCTGTCTCACGTCGCAGTGCCATGGACGCAGCAG1120.10047816842652982No Hit
GGATAAGGGCCACAGTGAAGCACGAAGGCCTGGGGAATCCTGACTGTGGCTC1120.10047816842652982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)