FastQCFastQC Report
Sat 31 Dec 2022
SRR5849072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110677
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC22642.045592128445838No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT21751.965177950251633No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3110.2809978586336818No Hit
GTCAATGGGGATGACATCCAATTATTATTTTTTTCTTTTATGTTTTTTGTGT2600.23491782393812627No Hit
CGACTCACTGCAACCTCTTCCTCCCAGGTTCAAGCATTTCTCCTGCCTCAGC2230.20148721053154678No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2080.18793425915050102No Hit
TCTTTGGGGGATCTTGACTTGGGGTAAGAATGTAAGGAGGGATAAGAACGAG1990.17980248832187357No Hit
TTAGGAGGGTAATAAAAAGAGCTGCTGCTGGACATTTATCAAAGATAGGCAC1650.1490824651915032No Hit
CACAATGGGGAAGAAAAAGTGTAACACTAAGGTGAACTTGCTTCCCTTTTCA1620.14637187491529405No Hit
GCAGGTGGGTCTGCAAGAGGGAAGACCTATAGATGTAAATATGGATGTAGAT1600.14456481473115462No Hit
GGCAAAGGGCGGCAAGGCCAAGCTGCGCAGCGTGAAGGAGCGAAAGCTGGAG1590.1436612846390849No Hit
AGACTCGGGACCTGGAAGAGAGCCTGCCCTGCAGAAGACCCTGATTCAATTA1570.14185422445494547No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1540.13914363417873632No Hit
TTCACTGGGATTATAGGCATGAGCCACTGCATCAGGCTAAAGAGCACATATT1530.1382401040866666No Hit
AAGCAAGGGATTCCTCCACGGAAGGGAAGACAAGTATCAACACCACTGGCAT1520.1373365739945969No Hit
CGTTCTGGGATTGGCAGTGCCTGCTCTTGTCAATACTCCTGCCCCAAAATGC1500.13552951381045744No Hit
AATGTCGGGATGAAGGAAAAAAAGCAGATCTGTTCAAATGCATGCTTATGTC1470.13281892353424832No Hit
TCAGCTGGGAACACATGATGTCATCAATAACCTACTGAAATTTATTCTACTT1460.1319153934421786No Hit
GACCTTGGGATCCTCATCGCTGTGGGCGCTGTCATGATGTTCGTTGGCTTCC1460.1319153934421786No Hit
CCTGCCCCTCCTGCCAGGCCCTCCCTCCCGGGTGGTGGCCGGCACCTCTGCT1440.13010833325803917No Hit
ATGTATGGGTATCCTTTGCCTAAAGAAATCTCTTTACAAAATCTAAACTATG1430.12920480316596944No Hit
GTCTACGGGGATTTTCTGGGAGCTATCTTGTCTACCTTGAGGTTCCTGAACA1410.12739774298183001No Hit
CTACTAGGGACATGTTATAGCAGTTCTTGGTATAGAGCAAACTAGAAGAACC1390.1255906827976906No Hit
TGTTTAGGGGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCT1380.12468715270562086No Hit
TTTTATGGGGTTTTCCCAATTCTACACATGGCAAAATGGAGACCCAGAGTCA1370.12378362261355115No Hit
GTACAGGGGACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGG1350.12197656242941171No Hit
ACTTTAGGGACCCCAGGCCCAAGCGCCTTGCTGTCACGCCCAACCTCTTTGG1350.12197656242941171No Hit
CTACGCTTGGGTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATC1340.121073032337342No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC1330.12016950224527229No Hit
TAGATGGGGGTTTGTTATTGGGGGTGTGGCCCAGGGCCCCAGTCAAGCCGTG1310.11836244206113285No Hit
ATGTCAGGGAGTCTATGACTGAATTTTGCTTTTATGGCTTTTGCTCTCAAAA1310.11836244206113285No Hit
TCAGTTGGGACAACTGTAGCAATCATGCCATTTGGCTAAATGTACAGAGTAC1290.11655538187699342No Hit
CATAACCGGGGTAAATGTGAATGGAAACTTGGAAATACTCGTTTTTATAAAC1290.11655538187699342No Hit
GGGCCAGGGGTCACTTTTCTGTGAGAGGTATTCATCCCTGACCTTGAAATAT1290.11655538187699342No Hit
AAAGGTGGGGAGAGCAAGCACTCTAGATGAAATAGCAGGTTATAGTAAACTG1290.11655538187699342No Hit
CGTATTGGGAAAGACACATGAGCCAGAGAAGCCGGCATGAGCAGAGATGCCC1270.11474832169285397No Hit
CAAAAAGGGGGGCCTCGGTCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCT1270.11474832169285397No Hit
GTGTGCGGGTAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTG1260.11384479160078426No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC1260.11384479160078426No Hit
TCCGCACAGGGGACTGCCTGCCGCGTGCGGAGCCGGAGCCCGAGCCTGAGTG1260.11384479160078426No Hit
CATGCAGGGGGGCCCTCAGGGCTGGGGAGCTCAGTTCCCATTGCTTGTGCTC1250.11294126150871454No Hit
GCCAAAGGGATGTGGGAGAGCAGATTGCTGTTCCCCGCAATGATGAATGGGC1250.11294126150871454No Hit
TCATCCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1250.11294126150871454No Hit
AGACTTGGGACCCTTCTGAACAAAACATGTACTTACTCTCCGAAAGGCATCT1240.11203773141664483No Hit
CTGAGTGAGGGGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCCGTATGA1230.11113420132457513No Hit
GGTAACGGGCGATCTCGACCTGGGGACTCAGATAGCTCAAGTGATTCTCAGT1220.11023067123250539No Hit
CAGTGCTGGGAGACTATTCAAAATACTTCCTCGGAGCTTGTATAGATTTCAG1210.10932714114043568No Hit
GAGCTGGGGAGAGGGAGGCGGCTGGCCCGGCGGCAGGCTCTCAGAACCGCTA1200.10842361104836597No Hit
ATCAATGGGATGATGACATCAAGAAGGTGGTGAAGCAGGCGTCGGAGGGCCC1200.10842361104836597No Hit
CCTCAAGGGGCCACATAAATGTCTTCTTTTGAGAAGTGTTTGTTTATGTCCT1200.10842361104836597No Hit
CATAAGGGGGGAAAGCAAAACTGTGGATAAGGGGGGACTAACTGTACCAGTA1190.10752008095629625No Hit
TCGCCAGGGAATTGTCTAAGGCATAGAGAGATTAGTAACTGTTGATACTGAT1190.10752008095629625No Hit
ATCACTGGGACCCCCTCTTCGTTATCTCCGTAACCCCAGTCCAAATAAACAA1180.10661655086422653No Hit
CTGGAGGGGGGCAATTGAAATGCAGGTGCAGTTTTCTGTTAATGTCATGCTG1180.10661655086422653No Hit
CTGTTTGGGAAAGTAAGAATATATTATTAGAACTTGCCCTGTGGAGACACCT1180.10661655086422653No Hit
TCGTCAGGGAAGAAGTCATTGAGTGGCTCGCCACTCAAAGTGAAGAACTCAA1180.10661655086422653No Hit
GTCTAAGGGGAGTGACAGATAATCAGGAGAAGAAAAGGAGATCCAGACTCAT1180.10661655086422653No Hit
TGCGCTAGGGGGGAGACCATCAGCTCATCCAGGAGGCCCTTGTCTTTGACAA1170.10571302077215682No Hit
CATAACGGGATGAAATGTTAACAATGCCTATTGTAATATAGAAAAAAACATT1170.10571302077215682No Hit
CGATGGGGGGCTTTCCTCTCGGCTGAGCCTCACGTTTGGAGCAGTTCTTGCG1170.10571302077215682No Hit
ATCATAGGGACTGCATTCATTCTGAGCCCCCTTCCTGTCCCCAGGGGAGGTG1170.10571302077215682No Hit
CGCTGGGCATGGAGGGATGTCCTTGTTGGCCTAACTGTGCTGAGCCAAGTGC1170.10571302077215682No Hit
CATAATGGGATTTCCTAACATTGTTTATGCATCAGACTATCTCAAAGGATTT1160.1048094906800871No Hit
TTGGTTGGGCAACACATCAAATAAAAGACACAGGATTTCAAAGAAAACCAAT1160.1048094906800871No Hit
GGCATAGGGGTATTCTTCCCCCCACCATGTGGCTGGGAGGACTTGGGAGGGG1160.1048094906800871No Hit
TCAAGGAGGGATATTTAATGGCAATGCAAAAGTCTTCCTGCTTCCGTGCTGA1150.10390596058801739No Hit
TGAACCGGGAACACAGTGGTAGGTATTTGTGTATATGAACGTAGACAAGGGA1150.10390596058801739No Hit
GCTCGCGGGGAGTCTGAGGAGTGCAGAGAAAAGATATATCCAAAAGGAGAAT1150.10390596058801739No Hit
CAAGCAGGGGCATTAGGTCTTGTGTTTGATGTGTTCTTGTGAATTTACTTTG1140.10300243049594768No Hit
ACAGGTTAGGGGGTGAAGGTCACAGCCCAAGGGTATAGTCTTACTGAAAGAC1140.10300243049594768No Hit
TACTATGGGGCTGCCAGACTTGGAAAGTCAATTAGTAGACTCATCGTTGTGG1140.10300243049594768No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1140.10300243049594768No Hit
GCAAGCGGATCTTATTCTTCATGTAATCCCACCTTCTAACAAAAAATAAATA1130.10209890040387794No Hit
CGTGGTGGGCAGGCAGTCAGTCAGAGTCTGAGTCAGACACTTCCCGAGAAAA1130.10209890040387794No Hit
AGTTATGGGATTTCCAAGTTACTCTAAAGAAACCCAGTATAAAATAACTAGT1130.10209890040387794No Hit
AGCAATGGGATGGCCTCAGCCAACTATCTTAGCTCCAGGTCACGGCCCCATC1130.10209890040387794No Hit
TCTATAGGGATTCCAAGAGATATACAGATTTTAAGAATAGATGCTCAGTAAT1130.10209890040387794No Hit
CATAAGGGATCTGTTAACGTTATTGGAATGCTGCTCAGTTCTCTGATCAGTG1120.10119537031180822No Hit
TTGGCTGGGTCTGCAGATGTTCTAGTTAAGCTAAAAAGTAAAAACTTTTTTA1120.10119537031180822No Hit
GGTTGCGGGACTTTAAAGCAAGGATTTAAACAAAGAAAACCATAAAAACAAA1120.10119537031180822No Hit
GTATTAGGGGGGAATCCAGAGAGGGGCAGTAGTAGAGTGGGCCAGTTGGGAC1110.10029184021973853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)