FastQCFastQC Report
Sat 31 Dec 2022
SRR5849068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178535
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT37402.0948273447783348No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC33871.8971070098300051No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3780.21172319153107233No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC3470.19435964936847117No Hit
CCGCATGGGGAAGGTTATGTAATATCTCTGTTGCTTTGTGTAGCTACCAGAC2690.15067073683031337No Hit
GGCACTGGGACTCGGACCTCTTTGAAGTCATCATAGTGTTTTTGGGAATAGA2490.13946845156411908No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT2490.13946845156411908No Hit
GACATGGGGGAAAAAGAGGACTGGGGCTTCAGCCTTCAGCATTCAGTATGTT2480.13890833730080937No Hit
TGTCAGGGTGCCAACGGGATATTGATACATTCAATTAAAAAAACTGGTACTA2290.12826616629792478No Hit
GACCACGGGGAGAGCTTTGTGGGATGGCAGAAATGCTCTACATTCTGATGGG2230.12490548071806648No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT2190.12266502366482762No Hit
ACTGTGGGGCTAGTTGAGGGATTGCCTTGCATAGTGTCTAAAAACTATGTTG2180.12210490940151791No Hit
GCATTCGGGGAAAATTCCCCTGGGATTGGAATTTTAAGACTCATGATGTCAA2010.11258296692525276No Hit
GGAAAAGGGGGGCACAGAGGGATTTAGAAGATGCATGTAATTCTGAGACCCA1990.11146273839863331No Hit
CCACAAGGGATCCTTATTTATTTATTTTTGCACTCATACTTGTTTTCTTTAT1920.1075419385554653No Hit
TGTACTGGGAGGGCCTTTTCTAGCATGTTCTTTTCTTTGGTTTTCTGATGTG1920.1075419385554653No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1900.10642171002884589No Hit
TGTGGCGGGCATTAGGTCAGATACATCAGGACATTTTGAACGTTTACTTGTG1810.10138068165905846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)