FastQCFastQC Report
Sat 31 Dec 2022
SRR5849067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175575
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT36162.059518724191941No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC32071.8265698419478855No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3430.1953581090701979No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC3410.19421899473159618No Hit
CCGCATGGGGAAGGTTATGTAATATCTCTGTTGCTTTGTGTAGCTACCAGAC2550.1452370781717215No Hit
GGCACTGGGACTCGGACCTCTTTGAAGTCATCATAGTGTTTTTGGGAATAGA2410.13726327780150932No Hit
GACATGGGGGAAAAAGAGGACTGGGGCTTCAGCCTTCAGCATTCAGTATGTT2350.1338459347857041No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT2330.13270682044710236No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT2260.1287199202619963No Hit
ACTGTGGGGCTAGTTGAGGGATTGCCTTGCATAGTGTCTAAAAACTATGTTG2120.12074611989178413No Hit
CCACAAGGGATCCTTATTTATTTATTTTTGCACTCATACTTGTTTTCTTTAT2090.11903744838388153No Hit
GACCACGGGGAGAGCTTTGTGGGATGGCAGAAATGCTCTACATTCTGATGGG2040.11618966253737718No Hit
GAGAAAGGGGTTAACATGGGTTTGGTAGTATATATGGGATGTAATAGGGTAG2000.11391143386017373No Hit
TGTCAGGGTGCCAACGGGATATTGATACATTCAATTAAAAAAACTGGTACTA1950.11106364801366937No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1920.10935497650576677No Hit
GTCTAGGGGGTTCATTGCAAAACCTTGCAGCTGAATTATCTGGGCAATAAAA1890.10764630499786416No Hit
TGTACTGGGAGGGCCTTTTCTAGCATGTTCTTTTCTTTGGTTTTCTGATGTG1870.10650719065926242No Hit
GACAAGGGGACTTTTTGCTAGATTATACCATGACATGCACACATTTCTCCAG1850.10536807632066068No Hit
TTTTATGGGAGATGGACTATAGTTTATCTGTTCAGTCACTGAAGGACATTTT1770.10081161896625374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)