FastQCFastQC Report
Sat 31 Dec 2022
SRR5849066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176760
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT36032.038357094365241No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC31141.7617107942973522No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3660.2070604209097081No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC3500.19800859923059516No Hit
CCGCATGGGGAAGGTTATGTAATATCTCTGTTGCTTTGTGTAGCTACCAGAC2810.1589726182394207No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT2420.13690880289658294No Hit
ACTGTGGGGCTAGTTGAGGGATTGCCTTGCATAGTGTCTAAAAACTATGTTG2380.13464584747680472No Hit
GACATGGGGGAAAAAGAGGACTGGGGCTTCAGCCTTCAGCATTCAGTATGTT2300.13011993663724825No Hit
GGCACTGGGACTCGGACCTCTTTGAAGTCATCATAGTGTTTTTGGGAATAGA2200.12446254808780267No Hit
GACAAGGGGACTTTTTGCTAGATTATACCATGACATGCACACATTTCTCCAG2100.11880515953835709No Hit
GGAAAAGGGGGGCACAGAGGGATTTAGAAGATGCATGTAATTCTGAGACCCA2060.11654220411857887No Hit
CCACAAGGGATCCTTATTTATTTATTTTTGCACTCATACTTGTTTTCTTTAT2040.11541072640868974No Hit
GCTCGGGACAAGAAAGATGTTGTTACATCAGGAGAATTGGAAATCAGTCAAA2030.1148449875537452No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1960.11088481556913328No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1950.11031907671418874No Hit
GCATTCGGGGAAAATTCCCCTGGGATTGGAATTTTAAGACTCATGATGTCAA1860.10522742701968772No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT1840.1040959493097986No Hit
TGTACTGGGAGGGCCTTTTCTAGCATGTTCTTTTCTTTGGTTTTCTGATGTG1810.10239873274496493No Hit
GCGTGTGGGGTTCCTCTTCATCGCCTTAATGTCTAAAGATCAGAAATCGCTG1780.10070151618013126No Hit
GACCACGGGGAGAGCTTTGTGGGATGGCAGAAATGCTCTACATTCTGATGGG1770.10013577732518669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)