FastQCFastQC Report
Sat 31 Dec 2022
SRR5849058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41515
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC16734.029868722148621No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT16063.868481271829459No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3440.8286161628327111No Hit
GCCAAAGGGACCAGGACACCAAGGTGGACATCCCATCCCTCATTCAAACCAT2730.6575936408527039No Hit
TGCGCAGGGAACAAATGAGGACCTACAGGGCAGAAAGGTCTCTCTCCTCCTG2470.5949656750572082No Hit
CTTTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2420.5829218354811514No Hit
TCGTTGGGGCGCTCGCGCCGAGGGGCTGCGAGAGTGACCGCGGCTGCTCCAG2370.5708779959050945No Hit
CATATAGGGATCCGACACCAGCCGCCTCCACCATGCCGCCGAAGTTCGACCC2120.5106587980248103No Hit
GTAACTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC2020.48657111887269666No Hit
CTTAGCGGGGAGGGTGGGATCTGGAAGCCAAGGGAGGAAAGAAGGATCACTG2000.4817535830422739No Hit
GACTCGGGATGCACATTCACGCTGTTTGTTTCATATATACAGGCATAAAATA1930.4648922076357943No Hit
TAACGCCGGGATAGTGGGTGCCATTCTCAACATATTGGTTTGCTAACTTTAA1930.4648922076357943No Hit
CCGTGCGGGGTCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAA1900.4576659038901602No Hit
AACCACAGGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGG1870.45043960014452605No Hit
ACGCGGGCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCCGT1870.45043960014452605No Hit
TATTGCGGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTAC1850.4456220643141033No Hit
TTATACGGGATCTTTACGCGGGGTAAAGTTCCTTACTTTGCTGATTTTTCAT1830.4408045284836806No Hit
GGGTCTGGGCCGATCTAACGCCGGGGCACTGCGAGCAGTAGCCCAAACAATC1770.42635192099241237No Hit
CAGCTGGGGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAG1720.41430808141635556No Hit
TTATATGGGGGTCACGTGACGCGGTGCCTGGCGCCGAGCCTCCCAAGATGGC1720.41430808141635556No Hit
AGTGTTGGGATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCT1650.3974467060098759No Hit
ATACGCGGGGTGTCAGTGAATGGGCAGCCATGCAGGTGGTGGAGACACGCGG1650.3974467060098759No Hit
ATCAGCGGGCAAATTTGACCTGGTGTTATTTTAGCCCCAAATTTATGACATT1610.3878116343490305No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1560.3757677947729736No Hit
AGCCATGGGAAGTGCAGTGAAAGAACTGCACGGTGAGTTTAAAACAACCGCT1550.37335902685776223No Hit
GACTACGGGGACCTCATTCTTCATGTCATTAAGCAATAGGTTGCAGCAAACA1540.3709502589425509No Hit
GAAAACGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG1500.3613151872817054No Hit
AGTCTGGGTGGGAACACTGATGGGGCCTTGGACAGGGCTTTCTCTCAGGTAG1490.35890641936649403No Hit
GAAGATGGCGAATTAGAAGATGGTGAAATAGACGATGCAGGATTTGAAGAAA1480.35649765145128265No Hit
TTAACTGGGATCCATGTCACCCACACACACCTGTATGCTGACAAACACGGAC1470.3540888835360713No Hit
GCTAGCGGGATCTTACAAGAGCATGCATAATGTTTACAATGAAAACAAGCGG1470.3540888835360713No Hit
TAGCCCAGGGATTTTGCAGTTGTTGAAATTGTCCCCGCAGTTTTCAATCATG1420.34204504396001445No Hit
GCACGAGGGACAAATATAATACATACTCATACAAAACTTTGGAAATTCAGTA1400.33722750812959174No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1380.33240997229916897No Hit
AACCACGGCCACAGGCAAGGGGGTTGCAGTGGCCGCCTGCACCTTGGATGAG1370.33000120438395764No Hit
CTTCACGGGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACT1370.33000120438395764No Hit
GTTCTAGGGATTGTAAAATATCTATTCCGTATTAGAAATAGCTAGTTGACAG1360.32759243646874625No Hit
GCACCAGGGACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG1350.32518366855353487No Hit
GAGCAGGGAATCTATGTACATATATTGTCTGTCCATATGTATTTGTAAATAG1340.3227749006383235No Hit
TTGCGCGGGGTTTGAACAGAAAATTCAAACAAGACTCTTTCCAATTTAAAGG1340.3227749006383235No Hit
GTATTTGGGAAGGTATGGTCGGAGGAGGAGCTCCTGCCTGCGGTTTTGTGTA1340.3227749006383235No Hit
GGTGTAGGGTGGACTGAACAGGCCTGATGGAGCTTGTGGCTGGCCTCAGATA1310.3155485968926894No Hit
TAAGTCGGGGACCACCTCTTTTTTAATGTATTTTTAGTCCACTTACAGCTTT1270.30591352523184395No Hit
TCTGAAGGGATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACC1230.2962784535709984No Hit
TGATTAGGGGGGAATCCTAACAGACTCTGCTTTCCTCTCTTCGTGGTTTGTC1210.2914609177405757No Hit
CTTTGCAGGGCGATCTCATTGCGGGATGGACTCTTCCGTCTCAATGCCTCCA1200.2890521498253643No Hit
CTACGTGGGACTGCATTTTAGTTTTGAAAATGACTTCCCCACCACCTAGAAA1200.2890521498253643No Hit
TGTGGAGGGACCTCACAATATGGAAAGACGGGACAACCTATGGAACTATCTG1190.28664338191015293No Hit
GCTCGACGGGAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTT1180.28423461399494154No Hit
ATTGTAGGGCAAAAGGTCCTGGAACAAGTTCTTCATCAGAACAATACAGTTT1160.2794170781645189No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1150.2770083102493075No Hit
CAATGTGGGGATTAATACTGGGTATGTTCTGGTAAATCCATCCTTATTGTAT1150.2770083102493075No Hit
TAATACGGGTGGGGTTGGTACTGTAATTCTCTCTGTTTGACAGATAAGAAAA1140.2745995423340961No Hit
GCAAATGGGGGGACTGTAAATATGTATATTTAACTTTGTATAGCCCATGTAC1140.2745995423340961No Hit
AGAGTCGGGTTCCTCACGAACCTCAGGATTGAGCAAGGTCTTTGTAATTTTT1110.267373238588462No Hit
GTAAACGGGGAGCTAAGTCAGCCAACACCTCTTGTTGCTCAGAGCTTCCATG1090.26255570275803924No Hit
CCCCTACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTTCTC1070.25773816692761653No Hit
CCCCCTCTACCTAAAACTCACAGCCCTCGCTGTCACTTTCCTAGGACTTCTA1070.25773816692761653No Hit
TAATAGGGGAACGAATCCCAGAACTTAAAGATTGGTTCTCTAAAATAAGACA1050.25292063109719376No Hit
ACACTAGGGATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACC1050.25292063109719376No Hit
ATATATGGGATCATGAGCCTCCTCAACAAGCCCAAGAGTGAGATGACCCCAG990.23846802360592556No Hit
CCGATTGGGACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTT990.23846802360592556No Hit
AGCAAAGGGGTGTCACCTGGATTTTTTTCTGCCCATGAATGTTGCCAGTCAG970.23365048777550285No Hit
GGCTATGGGAGATTGTATACAGGCATTTCACATTATTAGGACATGGAATGTT970.23365048777550285No Hit
TCATTGGGGGTGCTATCAACAGATGGCTATTGAACACTTGAAATGTGGCTAG970.23365048777550285No Hit
ATTTTAGGGACTATAGAATGCAGTGAAAAAGGCATTGATGGTAACATGCATG970.23365048777550285No Hit
TCTTTGGGGATATATCAAGATAGAAGATGAAAGAATGGGCTGCGTCTCATGG960.23124171986029146No Hit
CTCACTGGGGGACCCCAACTCTCCTGAGCGCGTGACCTTTGTGTCCAGAGCC950.2288329519450801No Hit
GTCATGGGACCATAACTGTTAAAACAATTTTTAGCTTTCCTCAAGTTAGTTA950.2288329519450801No Hit
GTGTAAGGGTAAATCAAGGTCATTTATAATCATTTTCCTTTTTTATTTAAGA930.22401541611465733No Hit
TGAAGGGATAACATAGCATGTTAGAGTTCTCTAGAGGGACAGAACTAACAGG910.21919788028423462No Hit
ATAAATGGGATTATATAGTTGTTTACATACTTATAAGTCTATCATTTAAAGA890.21438034445381188No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT880.2119715765386005No Hit
TACAGAGGGGTGGCCTGACTTGAGGGAACGATGTGTCTGTATTTCATGTGGC870.20956280862338916No Hit
TAACGGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTC850.2047452727929664No Hit
ATGCAAGGGGGGGCGGCAGAGCTCGGCGGCTGGGACTGGAGGACAGCGGTGG850.2047452727929664No Hit
TGAACAGGGGACTCTGATCCCATCACTGTGACGGTCCCTGTAGAAGTCTCCA790.19029266530169817No Hit
TGTCTAGGGGATCTAGGAACGGGGGTGTGTCTCAGCTAATCCAAAGGGAGTT780.1878838973864868No Hit
CTCTCAGGGCTAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATCATTT770.18547512947127545No Hit
GACATGGGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAAC770.18547512947127545No Hit
GTCTACGGGATGGATAATGTTGATTGGATAATGCACATCTCAGTTACAAGCA760.1830663615560641No Hit
CACTTTGGGACATTAATCCAGGTTCTGCCACTGGGGCATATAATGCCTTGGA740.17824882572564132No Hit
ACGTAGGGGGGAAGGTGAATGGAAGAGGGCACGTAGGGTGCTTCAAAGATAA730.17584005781042997No Hit
CCGGGGGCTCGCTCTGTCTATGCAGGGGCCCCAAAGCCCGCACTTTGTCCGT720.17343128989521858No Hit
AAGTAGGGGAGGCTAGTGTAACTCGCATCCCATTGCAGTGCCGTTTCTTGAC710.17102252198000722No Hit
TTGAGGGGATTCCCTATCTTTTTTAATATTAATAAAGTAAAAAAGATTCATA700.16861375406479587No Hit
TACGAAGGGATGGAATGTAAGAAGATGAAGGTGACTGGGATTCAGCGAGAGA700.16861375406479587No Hit
CTTATGGGGAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTC660.15897868240395038No Hit
TAAGTGGGGGTGTGGGGCAGCGCCTCGTACGCCGTGAGCCTGGCGGCGCTGC630.15175237865831628No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC620.1493436107431049No Hit
GTTCAAGGGATGTTAGCAGAATACTATAAAAGTTTGAAATTTTTAAAAAGTA620.1493436107431049No Hit
CGAGCAGGGTACCCTGTGTCACCCACAGCTCAGTGGCCTGCCTGCCGGCCCT620.1493436107431049No Hit
GCAAAAGGGATGCAAAAGGAATCGTGCTGGAAAAAGTAGGAGTTGAAGCCAA620.1493436107431049No Hit
ATTTTTGGGAGAGGCATTGACAACAGGGTTCGTAGAAGATTCAAGGGCCAGA600.14452607491268216No Hit
CGGGCCGGGCGATCTGAGTCTGGGAGATGGTGAAATGTGACCCTCGCCATGG590.14211730699747077No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT590.14211730699747077No Hit
GCTTCCGGGATAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTTTG590.14211730699747077No Hit
GTTCACGGGCTTCTCTCTAAGGAGAATGGCCCAGTCCTCTCCCAAGTCCACA590.14211730699747077No Hit
TGCCCAAGAAAAGAACTCTGCTGTCCAGGCACTGCTTGGCTTACTATCCCAG580.13970853908225944No Hit
CGAACGGGAGGTGCCCACCATCACGCCTGGCTAATTTTTGTATTTTTAGTAC580.13970853908225944No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC570.13729977116704806No Hit
CTTCTAGGGGACCGCCAGATTCTCCCTTAAGGAATTGACTTCAGCAGGGGTG570.13729977116704806No Hit
GCGTTGGGGAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGAG560.1348910032518367No Hit
TATTCCGGGAGGTTCTCTTTTACCTCTTCTTTATTAAATTCCTTGTAATTTG530.12766469950620257No Hit
CTCTTAGGGATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAA520.12525593159099122No Hit
GCCATCGGGATTTGAGTACATGTGAATCATGGATTTGGTATTCAACTTTTTC520.12525593159099122No Hit
TTTCACGGGGGCCCTTCAAAACAATGATCTTCAACAGTAAATGCAAGCACAA510.12284716367577984No Hit
GTAATGGGGGCTTCTGGATATCAGGAGCTACCTGGAAAAAGTCGCCACAGGC500.12043839576056847No Hit
ACTCATGGGACTTTGGGAGGCCGAGGTAGGCGGATCATGAGGTCAGGAGATC500.12043839576056847No Hit
GTAACAGGGAGCTCAGTTTCGGGCCTCCCTGTCGGGGGAGTTTTCTTGTCTG490.11802962784535709No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACTGTCTCTTATACAC490.11802962784535709No Hit
CTACCCCGGGCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCA460.110803324099723No Hit
ACACGGGGGAGGCCAGGCGGGGTGGCTCATGCTTCTAATCCCAGCACTTTGA460.110803324099723No Hit
CAGTATGGACAGTGTAACAACAAAACCAAAATGGCTGGACAGACTTCTTGTG460.110803324099723No Hit
AAGACAGGGAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAAC460.110803324099723No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATG450.10839455618451162No Hit
TTTATGGGGGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCTCAAACA440.10598578826930025No Hit
TACCGGGGACCGCAGCTGTGCCGTGGCACGATGGCGAGGAAGCCAGCCCCAA430.10357702035408889No Hit
TATTCCGGGACCCACAATCTGAGCACGCTACCAAATCTCAAAATATCCTAAG420.1011682524388775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)