FastQCFastQC Report
Sat 31 Dec 2022
SRR5849057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41400
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC15843.826086956521739No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15603.768115942028986No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3500.8454106280193237No Hit
TGCGCAGGGAACAAATGAGGACCTACAGGGCAGAAAGGTCTCTCTCCTCCTG2490.6014492753623188No Hit
GCCAAAGGGACCAGGACACCAAGGTGGACATCCCATCCCTCATTCAAACCAT2340.5652173913043479No Hit
CATATAGGGATCCGACACCAGCCGCCTCCACCATGCCGCCGAAGTTCGACCC2150.5193236714975845No Hit
CCGTGCGGGGTCAGTGGTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAA2120.5120772946859903No Hit
CTTTGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2110.5096618357487923No Hit
GACTCGGGATGCACATTCACGCTGTTTGTTTCATATATACAGGCATAAAATA2090.5048309178743962No Hit
ATCAGCGGGCAAATTTGACCTGGTGTTATTTTAGCCCCAAATTTATGACATT2080.5024154589371981No Hit
CTTAGCGGGGAGGGTGGGATCTGGAAGCCAAGGGAGGAAAGAAGGATCACTG2070.5No Hit
ACGCGGGCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCCGT2030.49033816425120774No Hit
TAACGCCGGGATAGTGGGTGCCATTCTCAACATATTGGTTTGCTAACTTTAA1960.47342995169082125No Hit
GGGTCTGGGCCGATCTAACGCCGGGGCACTGCGAGCAGTAGCCCAAACAATC1940.4685990338164251No Hit
CAGCTGGGGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAG1840.4444444444444444No Hit
AGTGTTGGGATATTTGACATTCTTCACTGTCTGTTGTTTACCTACCGTAGCT1790.4323671497584541No Hit
GAAAACGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG1780.42995169082125606No Hit
ATACGCGGGGTGTCAGTGAATGGGCAGCCATGCAGGTGGTGGAGACACGCGG1770.4275362318840579No Hit
GAGCAGGGAATCTATGTACATATATTGTCTGTCCATATGTATTTGTAAATAG1740.4202898550724638No Hit
GTAACTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1740.4202898550724638No Hit
TCGTTGGGGCGCTCGCGCCGAGGGGCTGCGAGAGTGACCGCGGCTGCTCCAG1730.41787439613526567No Hit
GACTACGGGGACCTCATTCTTCATGTCATTAAGCAATAGGTTGCAGCAAACA1720.41545893719806765No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1670.40338164251207725No Hit
AACCACAGGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGG1620.391304347826087No Hit
TTATACGGGATCTTTACGCGGGGTAAAGTTCCTTACTTTGCTGATTTTTCAT1580.38164251207729466No Hit
AGTCTGGGTGGGAACACTGATGGGGCCTTGGACAGGGCTTTCTCTCAGGTAG1570.37922705314009664No Hit
GCACCAGGGACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG1490.35990338164251207No Hit
TATTGCGGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTAC1490.35990338164251207No Hit
AGCCATGGGAAGTGCAGTGAAAGAACTGCACGGTGAGTTTAAAACAACCGCT1490.35990338164251207No Hit
CTTTGCAGGGCGATCTCATTGCGGGATGGACTCTTCCGTCTCAATGCCTCCA1470.35507246376811596No Hit
GTTCTAGGGATTGTAAAATATCTATTCCGTATTAGAAATAGCTAGTTGACAG1460.3526570048309179No Hit
GCTCGACGGGAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTT1450.3502415458937198No Hit
TTATATGGGGGTCACGTGACGCGGTGCCTGGCGCCGAGCCTCCCAAGATGGC1410.34057971014492755No Hit
TAGCCCAGGGATTTTGCAGTTGTTGAAATTGTCCCCGCAGTTTTCAATCATG1330.321256038647343No Hit
GTATTTGGGAAGGTATGGTCGGAGGAGGAGCTCCTGCCTGCGGTTTTGTGTA1320.3188405797101449No Hit
TTGCGCGGGGTTTGAACAGAAAATTCAAACAAGACTCTTTCCAATTTAAAGG1310.3164251207729468No Hit
TTAACTGGGATCCATGTCACCCACACACACCTGTATGCTGACAAACACGGAC1290.3115942028985507No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1290.3115942028985507No Hit
CCGATTGGGACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTT1290.3115942028985507No Hit
GCAAATGGGGGGACTGTAAATATGTATATTTAACTTTGTATAGCCCATGTAC1290.3115942028985507No Hit
TGATTAGGGGGGAATCCTAACAGACTCTGCTTTCCTCTCTTCGTGGTTTGTC1250.30193236714975846No Hit
ATTGTAGGGCAAAAGGTCCTGGAACAAGTTCTTCATCAGAACAATACAGTTT1240.2995169082125604No Hit
GTCATGGGACCATAACTGTTAAAACAATTTTTAGCTTTCCTCAAGTTAGTTA1240.2995169082125604No Hit
GCTAGCGGGATCTTACAAGAGCATGCATAATGTTTACAATGAAAACAAGCGG1220.2946859903381642No Hit
GGTGTAGGGTGGACTGAACAGGCCTGATGGAGCTTGTGGCTGGCCTCAGATA1220.2946859903381642No Hit
TAATAGGGGAACGAATCCCAGAACTTAAAGATTGGTTCTCTAAAATAAGACA1210.2922705314009662No Hit
CAATGTGGGGATTAATACTGGGTATGTTCTGGTAAATCCATCCTTATTGTAT1210.2922705314009662No Hit
TGTGGAGGGACCTCACAATATGGAAAGACGGGACAACCTATGGAACTATCTG1200.2898550724637681No Hit
GCACGAGGGACAAATATAATACATACTCATACAAAACTTTGGAAATTCAGTA1180.28502415458937197No Hit
CTTCACGGGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACT1160.28019323671497587No Hit
TAAGTCGGGGACCACCTCTTTTTTAATGTATTTTTAGTCCACTTACAGCTTT1140.2753623188405797No Hit
AACCACGGCCACAGGCAAGGGGGTTGCAGTGGCCGCCTGCACCTTGGATGAG1140.2753623188405797No Hit
CTACGTGGGACTGCATTTTAGTTTTGAAAATGACTTCCCCACCACCTAGAAA1130.27294685990338163No Hit
ATATATGGGATCATGAGCCTCCTCAACAAGCCCAAGAGTGAGATGACCCCAG1120.2705314009661836No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1100.26570048309178745No Hit
GTAAACGGGGAGCTAAGTCAGCCAACACCTCTTGTTGCTCAGAGCTTCCATG1100.26570048309178745No Hit
AGCAAAGGGGTGTCACCTGGATTTTTTTCTGCCCATGAATGTTGCCAGTCAG1090.2632850241545894No Hit
GGCTATGGGAGATTGTATACAGGCATTTCACATTATTAGGACATGGAATGTT1090.2632850241545894No Hit
ACACTAGGGATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACC1080.26086956521739135No Hit
CCCCTACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTTCTC1060.25603864734299514No Hit
ATTTTAGGGACTATAGAATGCAGTGAAAAAGGCATTGATGGTAACATGCATG1050.25362318840579706No Hit
GAAGATGGCGAATTAGAAGATGGTGAAATAGACGATGCAGGATTTGAAGAAA1040.25120772946859904No Hit
AGAGTCGGGTTCCTCACGAACCTCAGGATTGAGCAAGGTCTTTGTAATTTTT1040.25120772946859904No Hit
TCTTTGGGGATATATCAAGATAGAAGATGAAAGAATGGGCTGCGTCTCATGG1030.24879227053140096No Hit
AAGTAGGGGAGGCTAGTGTAACTCGCATCCCATTGCAGTGCCGTTTCTTGAC960.2318840579710145No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC960.2318840579710145No Hit
GACATGGGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAAC960.2318840579710145No Hit
TCATTGGGGGTGCTATCAACAGATGGCTATTGAACACTTGAAATGTGGCTAG950.22946859903381642No Hit
TAATACGGGTGGGGTTGGTACTGTAATTCTCTCTGTTTGACAGATAAGAAAA950.22946859903381642No Hit
ATAAATGGGATTATATAGTTGTTTACATACTTATAAGTCTATCATTTAAAGA930.2246376811594203No Hit
TTGAGGGGATTCCCTATCTTTTTTAATATTAATAAAGTAAAAAAGATTCATA920.2222222222222222No Hit
GTGTAAGGGTAAATCAAGGTCATTTATAATCATTTTCCTTTTTTATTTAAGA920.2222222222222222No Hit
TCTGAAGGGATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACC920.2222222222222222No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC900.21739130434782608No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT870.2101449275362319No Hit
GTCTACGGGATGGATAATGTTGATTGGATAATGCACATCTCAGTTACAAGCA870.2101449275362319No Hit
TAACGGAGGGGCAGATAATTTTTAGCTTAGGGCCTGGTGGCTAGGTCGGTTC850.20531400966183577No Hit
CTTATGGGGAACTATTTATATTATCCTAACTACTACCGCATTCCTACTACTC850.20531400966183577No Hit
TGTCTAGGGGATCTAGGAACGGGGGTGTGTCTCAGCTAATCCAAAGGGAGTT830.20048309178743962No Hit
ATGCAAGGGGGGGCGGCAGAGCTCGGCGGCTGGGACTGGAGGACAGCGGTGG830.20048309178743962No Hit
TACAGAGGGGTGGCCTGACTTGAGGGAACGATGTGTCTGTATTTCATGTGGC790.19082125603864733No Hit
CCGGGGGCTCGCTCTGTCTATGCAGGGGCCCCAAAGCCCGCACTTTGTCCGT790.19082125603864733No Hit
CCCCCTCTACCTAAAACTCACAGCCCTCGCTGTCACTTTCCTAGGACTTCTA780.18840579710144928No Hit
TGAAGGGATAACATAGCATGTTAGAGTTCTCTAGAGGGACAGAACTAACAGG770.1859903381642512No Hit
TACGAAGGGATGGAATGTAAGAAGATGAAGGTGACTGGGATTCAGCGAGAGA770.1859903381642512No Hit
TGAACAGGGGACTCTGATCCCATCACTGTGACGGTCCCTGTAGAAGTCTCCA740.17874396135265702No Hit
ACGTAGGGGGGAAGGTGAATGGAAGAGGGCACGTAGGGTGCTTCAAAGATAA730.17632850241545894No Hit
CACTTTGGGACATTAATCCAGGTTCTGCCACTGGGGCATATAATGCCTTGGA710.17149758454106281No Hit
CTCTCAGGGCTAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATCATTT700.16908212560386474No Hit
GCCATCGGGATTTGAGTACATGTGAATCATGGATTTGGTATTCAACTTTTTC680.16425120772946858No Hit
CGAGCAGGGTACCCTGTGTCACCCACAGCTCAGTGGCCTGCCTGCCGGCCCT660.15942028985507245No Hit
CTCACTGGGGGACCCCAACTCTCCTGAGCGCGTGACCTTTGTGTCCAGAGCC650.1570048309178744No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT630.15217391304347827No Hit
GCAAAAGGGATGCAAAAGGAATCGTGCTGGAAAAAGTAGGAGTTGAAGCCAA620.1497584541062802No Hit
GTAATGGGGGCTTCTGGATATCAGGAGCTACCTGGAAAAAGTCGCCACAGGC610.1473429951690821No Hit
GCGTTGGGGAATCCCAGCACTTTGGGAGGCTGAGGCGGGCAGATCACCTGAG610.1473429951690821No Hit
ATTTTTGGGAGAGGCATTGACAACAGGGTTCGTAGAAGATTCAAGGGCCAGA590.14251207729468598No Hit
GCTTCCGGGATAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTTTG580.14009661835748793No Hit
TAAGTGGGGGTGTGGGGCAGCGCCTCGTACGCCGTGAGCCTGGCGGCGCTGC570.13768115942028986No Hit
CGGGCCGGGCGATCTGAGTCTGGGAGATGGTGAAATGTGACCCTCGCCATGG570.13768115942028986No Hit
CGAACGGGAGGTGCCCACCATCACGCCTGGCTAATTTTTGTATTTTTAGTAC550.13285024154589373No Hit
ACACGGGGGAGGCCAGGCGGGGTGGCTCATGCTTCTAATCCCAGCACTTTGA530.12801932367149757No Hit
ACTGTTGGGGAGGAGTATGAGTACCCCTCCTAGAGGACCCAGCCGCCATCAG510.12318840579710144No Hit
TGCCCAAGAAAAGAACTCTGCTGTCCAGGCACTGCTTGGCTTACTATCCCAG510.12318840579710144No Hit
CAGTATGGACAGTGTAACAACAAAACCAAAATGGCTGGACAGACTTCTTGTG510.12318840579710144No Hit
ATAATCGGGGCCTCATTATCATTCTTGAGTCAGATACTTCTGTTGATGGGAG510.12318840579710144No Hit
CTACCCCGGGCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCA500.12077294685990338No Hit
CTCTTAGGGATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAA500.12077294685990338No Hit
AAGACAGGGAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAAC490.11835748792270531No Hit
CTAGCTGGGCCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.11835748792270531No Hit
GTTCACGGGCTTCTCTCTAAGGAGAATGGCCCAGTCCTCTCCCAAGTCCACA490.11835748792270531No Hit
TTTCACGGGGGCCCTTCAAAACAATGATCTTCAACAGTAAATGCAAGCACAA480.11594202898550725No Hit
CGAACAGGGCGGAGGCGTTGATGTCCCCCTGTCTGGCCACGTCCTTCTCCTT470.11352657004830918No Hit
GTTCAAGGGATGTTAGCAGAATACTATAAAAGTTTGAAATTTTTAAAAAGTA470.11352657004830918No Hit
CTTCTAGGGGACCGCCAGATTCTCCCTTAAGGAATTGACTTCAGCAGGGGTG460.1111111111111111No Hit
AGCACCGGGATTTCCATTTTAATGGTTCTTTTATACTCTAAAATATTGACTT460.1111111111111111No Hit
TATTCCGGGAGGTTCTCTTTTACCTCTTCTTTATTAAATTCCTTGTAATTTG450.10869565217391304No Hit
CACACGGGACCATTTAAAAATTGTCACTGCTCTTTTTCATTACTGTAGATAA450.10869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACTGTCTCTTATACAC450.10869565217391304No Hit
GTAGCTGGGGTTTTGATATTAGGAGCCAGCCCATGTAATGCCTCTCAGGCTT440.10628019323671498No Hit
ACTACCGGGGACAAACCCTCATGAATTTGTATAAGAACTTACAAACAAATAT440.10628019323671498No Hit
GCAATAGGGAGGGAAGGCAGGGCTGCCCAGAAGTCTCAGAGGCACCTCACGC440.10628019323671498No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACCTCGTATGC430.10386473429951691No Hit
TTTATGGGGGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCTCAAACA430.10386473429951691No Hit
GTAACAGGGAGCTCAGTTTCGGGCCTCCCTGTCGGGGGAGTTTTCTTGTCTG430.10386473429951691No Hit
AACAGTGGGGCCTGACCAACATGGTGAAACCCCGTCTCTACTAAAAAAATAT430.10386473429951691No Hit
ACTCATGGGACTTTGGGAGGCCGAGGTAGGCGGATCATGAGGTCAGGAGATC430.10386473429951691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)