FastQCFastQC Report
Sat 31 Dec 2022
SRR5849056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219593
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC28231.2855601043749119No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT24761.1275404953709818No Hit
AATTACGGGACAGAAGAAGGAACAGATGTTCAGAGATTTCCTAGCTATCCCA4510.20537995291288885No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3750.17077047082557278No Hit
TTGTGTGGGCTCCACTACATTCTACATTTGCATCATAGCCACAGATTTTTTT3720.16940430705896817No Hit
ACATCAGGGGTGCGGCTCTACACGGAGCCGCTGCTGGTGAACCTGCCGACAC3660.16667197952575902No Hit
AACTCTGGGAGCTTGCACAAGTGGGCTGAAACAGCCCACATGCATGTTTATT3210.1461795230266903No Hit
GCTTCCGGGGCATGCAGATTTTGCAGGTGTGGAACGCCTAGCTCTTGTCACA3110.14162564380467502No Hit
CCATTTGGGGGTCTTGGAAAAGATTGTCCCTTCTCTTGCTGTTCTAATGCAT3110.14162564380467502No Hit
CTCTGAGGGACCTGCTTACCAGCCGGGACCAGAGCTACCAGTTCAAGACGGG3070.1398040921158689No Hit
TAACGGTGGGGGCTAACAGGCTGCAGAATGGCAGGAAGGACTCGCTAGAGAT2920.132973273282846No Hit
TGTTACGGGACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCG2890.1316071095162414No Hit
GCACACGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA2890.1316071095162414No Hit
GGAACTGGGCTAGCTCCCTCATATGTGCAGTTCACAATAGGGTTCATACTTC2860.13024094574963682No Hit
TGTAAAGGGCTGCCTCCGTTATGAGATCAAGGATATCCATGTGCCACCCCGG2790.12705323029422613No Hit
ACAAAGGGAATCTAATTTTAATGTACTGATAAAAACAAACATGGTTGTCCTT2790.12705323029422613No Hit
GTTAACGGGCTGGGTAATTTATAAAGAAAAGAGGTTTAACTGACTCACAGTT2780.1265978423720246No Hit
GGCACTGCGGGAAAGGCAGTGGAGCCCCACACTTCTGTTATTCCTCTATGCT2770.12614245444982308No Hit
GTAATGGGGGGTGGCCCGCCATGTTCTTCTCCGCGGCGCTCCGGGCCCGGGC2760.12568706652762157No Hit
GCTTGAGGGAATATCAAATTACTATTTATTCATAACAATTGATTTGATGCTA2710.12341012691661393No Hit
TTTCAAGGGATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCA2650.12067779938340477No Hit
GACATGGGGGGAAACAGGTAGCGAGGAAAGTTTAAAAGCTATAGCAGATTTT2630.1197670235390017No Hit
GCTACCGGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG2580.11749008392799407No Hit
TTTCCTGGGGGGGGAGAAAATACATGAATTATGTAATATTGGTTATTGTAAT2580.11749008392799407No Hit
TCCATGGGGATTCAATCCACCACTTCTCTGTGAAACACTCTACCTTGTTTTT2560.116579308083591No Hit
CGTTCAGGGGCGACTGTATGGAGAAGAACTGTTTGTCATTCAGTGCCGTGGG2560.116579308083591No Hit
TTTCGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2540.11566853223918794No Hit
AGTATAGGGACACCACATTTTCAATTGTGTTAAAACAATTGATCAATTCACC2540.11566853223918794No Hit
CTGTAGTGGGGAATCTTTGTTCTTTTTACATTTTATTGTATTTTAGACGGGG2530.11521314431698643No Hit
GTGCCAGGGCATGCATTGATTATAAAAATTCAGTATTAATTTTTTCTGATCT2530.11521314431698643No Hit
AGGTCTGGGGAAAAATTTAGTTCTAAAAAATGCAACCTTGCTAAATTGAGTA2520.1147577563947849No Hit
TAGGATGGGAGAGGAGGAAGCGGAAAGTAGTTTTGAGTAATACTTTGTTCAT2520.1147577563947849No Hit
ATACCTGGATGAGATCAAAGCAGGAGAAGAATTTAGAAGCTTCAAAGATTCT2490.11339159262818031No Hit
GCGCAGGGATATCAGGCAAGGTTTTGATATTCAGGATTTAGGAAGAACTCAT2490.11339159262818031No Hit
GCCGGTGGGGGTGTTCTGAGGTAGTTGGAGATTTATACTTTGTACACAAATA2480.11293620470597877No Hit
TAGCAAGGGTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATT2470.11248081678377726No Hit
AACTTAGGGATGCAAGAAATACTGTGGACTCGAATGAGGAGGATGGCGGTGG2460.11202542886157574No Hit
GCTAATGGGATTGTCACTCATTTTTCTAGTGAGTTGATATAAACTTATACTA2440.11111465301717267No Hit
ATGCACGGGAGAGAAGAGCCAAAAGGAGAAGAGAATGCTTTTATTTTTAATA2440.11111465301717267No Hit
TACTAGGGGTATGTTGAATCTTAAAACACTGCATCATGTTGCAAAATGTCCA2410.1097484892505681No Hit
AGTTCTGGGGGATTACTTGTAGAGTCCAATTCTTAGCAATGGAATCCCTCTA2390.10883771340616505No Hit
AATACAGGGAGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAA2370.10792693756176198No Hit
TGAAGGGGTCAGGTGTCTAGGTCACCCTCCAAGTCAGTGACATATATTCAGT2360.10747154963956047No Hit
TTAGGCGGGTCTGACAAGGGGGACAAATGTTCTTCCAAAGAACATTGAATAC2350.10701616171735893No Hit
CTCAGAGGGGCAGCTGGACCCATGTGAAGATACCTCACTCCAGCCCACTTCC2340.10656077379515741No Hit
ATCCTCGGGATATCACATACATTCATATATCTCAAGAAATGAAAGCAATGAA2330.10610538587295587No Hit
TGACGTTGGGGTAAATAGAAGCAAGCTATTATTAACAACTTTTGTGTACTTA2320.10564999795075435No Hit
GGGACCGGGATGGGCTCCAGAGTGTGTTTTAAAAAATACCCACTATTTCATT2320.10564999795075435No Hit
TGCAGGGAGGATAGGGAAAAGCTATGCTTTCCATAATTAGGAATTTTGTTAT2310.10519461002855282No Hit
GCTACCGGGATGCTCCATCAACTCTGGAAAGCAGAAGGATGTGACTAAGAGC2300.10473922210635131No Hit
CAATTTGGGATAAATGGAAAGACTTTCAGATTTTAGCTACTTTCGTAGTCTT2300.10473922210635131No Hit
TAAGTGGGATGAGCAGGCTGAGAGTGACATCATGAAGAGACAGCCCAGAAAT2300.10473922210635131No Hit
TATCATGGGAATATGAATATAAATCTTGTAACTTTTAAAATTGTAATGGAGG2270.1033730583397467No Hit
TTGCTCCGGGCGGGAGCCACGGCTTGTTGTGGTTACTGACCCCAGGGCTGAC2270.1033730583397467No Hit
AACTCAGGGAGTCCCATCTGAGCCAATTCAGTTCATGCGTTGAATCACAATA2260.10291767041754518No Hit
AATCACGGGATACTCACTCATTTAGTTGCCGTTTCTGGTGTTCCTCATTTCT2260.10291767041754518No Hit
TCATAGGGGGAACTGCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGG2250.10246228249534364No Hit
TGACCCGGGATTTTGATCAAGTTTCTCAAATTTTATGCTACATGTATATGAG2240.10200689457314215No Hit
TGATTTGGGCAGGGCAGCCCCCCACAAATAAGAATTATCCAGCCCCAAAATG2240.10200689457314215No Hit
TGATTAGGGATCCCATGGATTCCAGGTGACTAAAGAATACTTATTTCTTAGG2240.10200689457314215No Hit
TGACAGGGGAAATCACAAGGACTTGAAACTACAAAATGTATACTTAGAGCCA2230.1015515066509406No Hit
GTTACAGGGATATAGTTTTTTTTTAAAGGAATGTTTCCCTAGTCTTTTTGCC2220.10109611872873908No Hit
GGCTTTGGGAAGTTTTTTAAGAACCATGAAAAGCTAACCATTGACAATTTAA2220.10109611872873908No Hit
ATTCTTGGGACGTTAATTCTAGGTGATGTTCATTCTATAATGAGTGTTCATT2210.10064073080653754No Hit
TTGCAGGGGAATAGTCTTAACTTTGGACTTAAGCATGGCAGGGGACTTTGAT2200.10018534288433602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)