FastQCFastQC Report
Sat 31 Dec 2022
SRR5849055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216369
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC27171.255725173199488No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT23341.078712754599781No Hit
AATTACGGGACAGAAGAAGGAACAGATGTTCAGAGATTTCCTAGCTATCCCA4610.21306194510304155No Hit
ACATCAGGGGTGCGGCTCTACACGGAGCCGCTGCTGGTGAACCTGCCGACAC4110.18995327426757067No Hit
TTGTGTGGGCTCCACTACATTCTACATTTGCATCATAGCCACAGATTTTTTT3690.17054199076577514No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3330.1539037477642361No Hit
ACAAAGGGAATCTAATTTTAATGTACTGATAAAAACAAACATGGTTGTCCTT3270.1511307072639796No Hit
AACTCTGGGAGCTTGCACAAGTGGGCTGAAACAGCCCACATGCATGTTTATT3230.1492820135971419No Hit
CCATTTGGGGGTCTTGGAAAAGATTGTCCCTTCTCTTGCTGTTCTAATGCAT3090.14281158576321007No Hit
TTGCTCCGGGCGGGAGCCACGGCTTGTTGTGGTTACTGACCCCAGGGCTGAC3080.14234941234650067No Hit
GGAACTGGGCTAGCTCCCTCATATGTGCAGTTCACAATAGGGTTCATACTTC3010.13911419842953474No Hit
TGTTACGGGACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCG3000.1386520250128253No Hit
GCTTGAGGGAATATCAAATTACTATTTATTCATAACAATTGATTTGATGCTA2930.13541681109585937No Hit
GGCACTGCGGGAAAGGCAGTGGAGCCCCACACTTCTGTTATTCCTCTATGCT2900.13403029084573115No Hit
GTAATGGGGGGTGGCCCGCCATGTTCTTCTCCGCGGCGCTCCGGGCCCGGGC2850.13171942376218404No Hit
TAACGGTGGGGGCTAACAGGCTGCAGAATGGCAGGAAGGACTCGCTAGAGAT2840.13125725034547464No Hit
GCACACGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA2810.1298707300953464No Hit
GACATGGGGGGAAACAGGTAGCGAGGAAAGTTTAAAAGCTATAGCAGATTTT2760.12755986301179928No Hit
GCTTCCGGGGCATGCAGATTTTGCAGGTGTGGAACGCCTAGCTCTTGTCACA2750.12709768959508985No Hit
CTCTGAGGGACCTGCTTACCAGCCGGGACCAGAGCTACCAGTTCAAGACGGG2740.12663551617838045No Hit
TTTCGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2710.1252489959282522No Hit
CTCAGAGGGGCAGCTGGACCCATGTGAAGATACCTCACTCCAGCCCACTTCC2710.1252489959282522No Hit
TTTCAAGGGATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCA2680.12386247567812395No Hit
GTTAACGGGCTGGGTAATTTATAAAGAAAAGAGGTTTAACTGACTCACAGTT2650.12247595542799569No Hit
TACTAGGGGTATGTTGAATCTTAAAACACTGCATCATGTTGCAAAATGTCCA2630.12155160859457685No Hit
TCCATGGGGATTCAATCCACCACTTCTCTGTGAAACACTCTACCTTGTTTTT2580.11924074151102976No Hit
TGTAAAGGGCTGCCTCCGTTATGAGATCAAGGATATCCATGTGCCACCCCGG2580.11924074151102976No Hit
GCCGGTGGGGGTGTTCTGAGGTAGTTGGAGATTTATACTTTGTACACAAATA2580.11924074151102976No Hit
AATACAGGGAGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAA2570.11877856809432036No Hit
AGGTCTGGGGAAAAATTTAGTTCTAAAAAATGCAACCTTGCTAAATTGAGTA2560.11831639467761094No Hit
CGTTCAGGGGCGACTGTATGGAGAAGAACTGTTTGTCATTCAGTGCCGTGGG2560.11831639467761094No Hit
ATGCACGGGAGAGAAGAGCCAAAAGGAGAAGAGAATGCTTTTATTTTTAATA2560.11831639467761094No Hit
TAGGATGGGAGAGGAGGAAGCGGAAAGTAGTTTTGAGTAATACTTTGTTCAT2490.11508118076064501No Hit
GCTACCGGGATGCTCCATCAACTCTGGAAAGCAGAAGGATGTGACTAAGAGC2480.1146190073439356No Hit
ATTCTTGGGACGTTAATTCTAGGTGATGTTCATTCTATAATGAGTGTTCATT2450.11323248709380734No Hit
GAAACAGGGAACTCCTTCATGCAATAAACTGAAAAGAGCCATGCTGTCTAGT2430.1123081402603885No Hit
TAGCGACGGGGAACCCAGTACCTCAGATGGAAATGCAGAAATCACCGTCTTC2410.11138379342696966No Hit
GCTAATGGGATTGTCACTCATTTTTCTAGTGAGTTGATATAAACTTATACTA2390.11045944659355082No Hit
TTGCAGGGGAATAGTCTTAACTTTGGACTTAAGCATGGCAGGGGACTTTGAT2380.10999727317684141No Hit
TGACGTTGGGGTAAATAGAAGCAAGCTATTATTAACAACTTTTGTGTACTTA2370.10953509976013201No Hit
AGTTCTGGGGGATTACTTGTAGAGTCCAATTCTTAGCAATGGAATCCCTCTA2370.10953509976013201No Hit
GCGCAGGGATATCAGGCAAGGTTTTGATATTCAGGATTTAGGAAGAACTCAT2370.10953509976013201No Hit
GCTACCGGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG2350.10861075292671317No Hit
AGGCATGGGATATTATGTGTTACACTGATTGATTTTCACATGTTGAACAATC2350.10861075292671317No Hit
TGCTACGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGG2340.10814857951000376No Hit
ATACCTGGATGAGATCAAAGCAGGAGAAGAATTTAGAAGCTTCAAAGATTCT2340.10814857951000376No Hit
GGGACCGGGATGGGCTCCAGAGTGTGTTTTAAAAAATACCCACTATTTCATT2340.10814857951000376No Hit
GTGCCAGGGCATGCATTGATTATAAAAATTCAGTATTAATTTTTTCTGATCT2330.10768640609329433No Hit
AGTGTGGGCTTCTTTTCACTATCTTATAGCTTAATTGGTAATGAGCAGCAAA2320.10722423267658492No Hit
AACTCAGGGAGTCCCATCTGAGCCAATTCAGTTCATGCGTTGAATCACAATA2320.10722423267658492No Hit
ATCGCGGGGTGGCATTTCGAATGGAATAAATGGGGGCAATCTCTCATTTCTG2320.10722423267658492No Hit
ATCAAGGGGGTTCTTTCATTAAAAGACCAAAAAGAAGTTAAAACTTAAATTG2310.10676205925987547No Hit
TTTCCTGGGGGGGGAGAAAATACATGAATTATGTAATATTGGTTATTGTAAT2300.10629988584316608No Hit
TAACAGGGGAGCATTAAAAAGTAATGCTTTATATGATCTTGTTTTATTACAA2280.10537553900974723No Hit
TTAGGCGGGTCTGACAAGGGGGACAAATGTTCTTCCAAAGAACATTGAATAC2280.10537553900974723No Hit
TGAAGGGGTCAGGTGTCTAGGTCACCCTCCAAGTCAGTGACATATATTCAGT2280.10537553900974723No Hit
CTTTGGTGGGATCTTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCATAT2280.10537553900974723No Hit
CTACATGGGAGGATAGAAAATGAATTACAACTTAAATACATGTTGGAAGTGT2280.10537553900974723No Hit
CATAATGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACG2270.10491336559303782No Hit
TGCAGGGAGGATAGGGAAAAGCTATGCTTTCCATAATTAGGAATTTTGTTAT2260.10445119217632841No Hit
GTCCTCGGGATGGCTAGATCCCCAAAACCAGTTGTAACAAAAACAAAAATTG2250.10398901875961898No Hit
TATCATGGGAATATGAATATAAATCTTGTAACTTTTAAAATTGTAATGGAGG2240.10352684534290957No Hit
TAGCAAGGGTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATT2240.10352684534290957No Hit
CTAGCGTAGGGGGGCCATACCACAACCTCAGCTCAGCCCCTTGTCGGATACG2240.10352684534290957No Hit
TAGGTTGGGAAGAGAACACAGAACTAAACAAAATCAGGAAAACCCAATACAT2230.10306467192620014No Hit
TGATTAGGGATCCCATGGATTCCAGGTGACTAAAGAATACTTATTTCTTAGG2220.10260249850949073No Hit
TAGCAGGGGACTTTTATTTAAATTTTATTTGGATTAATGCGGGACTCCAGAT2220.10260249850949073No Hit
CAATTTGGGATAAATGGAAAGACTTTCAGATTTTAGCTACTTTCGTAGTCTT2220.10260249850949073No Hit
TCATAGGGGGAACTGCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGG2220.10260249850949073No Hit
TTGCTAGGGGTTACCTCTTTTATGGTTTTTAATTTTTATCAGGACTAACATT2210.10214032509278131No Hit
ATACTTGGGAAGCCATATTAGATTGCTTTGATCAGGCTGCCATTTGAGAAGC2210.10214032509278131No Hit
TGTCTGGGGACTAAGCAAAGCAATTTGAGTTTATGATTGTGTTGAGGTGGTC2210.10214032509278131No Hit
TCTAGGGGAAGTGCGAAATACTTTGTCTACCATGTTAGGAAAGTCTGCCACC2210.10214032509278131No Hit
AGTATAGGGACACCACATTTTCAATTGTGTTAAAACAATTGATCAATTCACC2210.10214032509278131No Hit
ACATCTGGGATTTGCTAATTGTGGAATCAAAAATTTTGATAACATTCTAACT2200.10167815167607189No Hit
TGTGCTGGGGATTTCAAGGCACATTATTATCATCAGTCTGGGTGAGGTATCT2190.10121597825936247No Hit
ATACAAGGGATAAAGTGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGAC2170.10029163142594363No Hit
TAAGTGGGATGAGCAGGCTGAGAGTGACATCATGAAGAGACAGCCCAGAAAT2170.10029163142594363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)