FastQCFastQC Report
Sat 31 Dec 2022
SRR5849054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217494
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC24721.1365830781538802No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT23911.099340671466799No Hit
AATTACGGGACAGAAGAAGGAACAGATGTTCAGAGATTTCCTAGCTATCCCA4050.1862120334354051No Hit
TTGTGTGGGCTCCACTACATTCTACATTTGCATCATAGCCACAGATTTTTTT3450.1586250655190488No Hit
ACATCAGGGGTGCGGCTCTACACGGAGCCGCTGCTGGTGAACCTGCCGACAC3350.15402723753298941No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3250.14942940954693004No Hit
AACTCTGGGAGCTTGCACAAGTGGGCTGAAACAGCCCACATGCATGTTTATT3120.14345223316505284No Hit
CCATTTGGGGGTCTTGGAAAAGATTGTCCCTTCTCTTGCTGTTCTAATGCAT3050.14023375357481127No Hit
GCTTCCGGGGCATGCAGATTTTGCAGGTGTGGAACGCCTAGCTCTTGTCACA2960.13609570838735782No Hit
GACATGGGGGGAAACAGGTAGCGAGGAAAGTTTAAAAGCTATAGCAGATTTT2940.13517614279014595No Hit
GGAACTGGGCTAGCTCCCTCATATGTGCAGTTCACAATAGGGTTCATACTTC2920.13425657719293405No Hit
CGTTCAGGGGCGACTGTATGGAGAAGAACTGTTTGTCATTCAGTGCCGTGGG2900.1333370115957222No Hit
ACAAAGGGAATCTAATTTTAATGTACTGATAAAAACAAACATGGTTGTCCTT2850.1310380976026925No Hit
GCACACGGGATGAGGAAGAAAACTGGCTTCAAGAATGAAAATTTCCATTTTA2840.13057831480408655No Hit
TGTAAAGGGCTGCCTCCGTTATGAGATCAAGGATATCCATGTGCCACCCCGG2740.12598048681802715No Hit
AGGTCTGGGGAAAAATTTAGTTCTAAAAAATGCAACCTTGCTAAATTGAGTA2720.1250609212208153No Hit
GTAATGGGGGGTGGCCCGCCATGTTCTTCTCCGCGGCGCTCCGGGCCCGGGC2710.12460113842220936No Hit
TGTTACGGGACGAACTGAAGAAGGCCGCCTATGACATCGAGGTCAACACCCG2670.1227620072277856No Hit
TACTAGGGGTATGTTGAATCTTAAAACACTGCATCATGTTGCAAAATGTCCA2660.12230222442917965No Hit
TTGCTCCGGGCGGGAGCCACGGCTTGTTGTGGTTACTGACCCCAGGGCTGAC2660.12230222442917965No Hit
GGCACTGCGGGAAAGGCAGTGGAGCCCCACACTTCTGTTATTCCTCTATGCT2620.12046309323475592No Hit
TGACCCGGGATTTTGATCAAGTTTCTCAAATTTTATGCTACATGTATATGAG2610.12000331043614995No Hit
TAGGATGGGAGAGGAGGAAGCGGAAAGTAGTTTTGAGTAATACTTTGTTCAT2600.11954352763754403No Hit
TCCATGGGGATTCAATCCACCACTTCTCTGTGAAACACTCTACCTTGTTTTT2580.11862396204033215No Hit
CTCAGAGGGGCAGCTGGACCCATGTGAAGATACCTCACTCCAGCCCACTTCC2570.1181641792417262No Hit
ATTCTTGGGACGTTAATTCTAGGTGATGTTCATTCTATAATGAGTGTTCATT2560.11770439644312027No Hit
CCACGGGCCGATCTATAGAAGGGAATAGCTTTTCTCAAATATACTTTTAGAA2530.11632504804730244No Hit
AGTTCTGGGGGATTACTTGTAGAGTCCAATTCTTAGCAATGGAATCCCTCTA2510.11540548245009058No Hit
GCTACCGGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTG2510.11540548245009058No Hit
TTTCAAGGGATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCA2510.11540548245009058No Hit
TAACGGTGGGGGCTAACAGGCTGCAGAATGGCAGGAAGGACTCGCTAGAGAT2450.11264678565845496No Hit
GCGCAGGGATATCAGGCAAGGTTTTGATATTCAGGATTTAGGAAGAACTCAT2440.11218700285984899No Hit
ATCCTCGGGATATCACATACATTCATATATCTCAAGAAATGAAAGCAATGAA2430.11172722006124307No Hit
TGATTAGGGATCCCATGGATTCCAGGTGACTAAAGAATACTTATTTCTTAGG2430.11172722006124307No Hit
ACTATGGGAGAAGTCTTGTGATGAAAATGGCACTTGGAAAAGGTTTTATTTT2430.11172722006124307No Hit
TCTAGGGGAAGTGCGAAATACTTTGTCTACCATGTTAGGAAAGTCTGCCACC2420.11126743726263713No Hit
TAGGTTGGGAAGAGAACACAGAACTAAACAAAATCAGGAAAACCCAATACAT2420.11126743726263713No Hit
TTGCAGGGGAATAGTCTTAACTTTGGACTTAAGCATGGCAGGGGACTTTGAT2380.10942830606821337No Hit
TGAAGGGGTCAGGTGTCTAGGTCACCCTCCAAGTCAGTGACATATATTCAGT2370.10896852326960745No Hit
TAGCGACGGGGAACCCAGTACCTCAGATGGAAATGCAGAAATCACCGTCTTC2360.10850874047100151No Hit
CTCTGAGGGACCTGCTTACCAGCCGGGACCAGAGCTACCAGTTCAAGACGGG2360.10850874047100151No Hit
AATACAGGGAGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAA2360.10850874047100151No Hit
GTTAACGGGCTGGGTAATTTATAAAGAAAAGAGGTTTAACTGACTCACAGTT2360.10850874047100151No Hit
TTTCGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2350.10804895767239556No Hit
GCTACCGGGATGCTCCATCAACTCTGGAAAGCAGAAGGATGTGACTAAGAGC2350.10804895767239556No Hit
TTTCCTGGGGGGGGAGAAAATACATGAATTATGTAATATTGGTTATTGTAAT2350.10804895767239556No Hit
GCTTGAGGGAATATCAAATTACTATTTATTCATAACAATTGATTTGATGCTA2340.10758917487378962No Hit
GAAACAGGGAACTCCTTCATGCAATAAACTGAAAAGAGCCATGCTGTCTAGT2330.10712939207518368No Hit
TATCATGGGAATATGAATATAAATCTTGTAACTTTTAAAATTGTAATGGAGG2310.10620982647797181No Hit
AGTATAGGGACACCACATTTTCAATTGTGTTAAAACAATTGATCAATTCACC2310.10620982647797181No Hit
GCTAATGGGATTGTCACTCATTTTTCTAGTGAGTTGATATAAACTTATACTA2280.104830478082154No Hit
GCCGGTGGGGGTGTTCTGAGGTAGTTGGAGATTTATACTTTGTACACAAATA2280.104830478082154No Hit
GCGACCGGGAATGAGGAGATGAAAATTAGTTTCTTGGAAAAAACATCTCTCA2280.104830478082154No Hit
TCTACAGGGGAAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA2270.10437069528354806No Hit
AACACCGGGAGCCTCACCCCACCTCAGCCCCAGTCCTGCCCCTAGCACAGCC2270.10437069528354806No Hit
TGTCTGGGGACTAAGCAAAGCAATTTGAGTTTATGATTGTGTTGAGGTGGTC2270.10437069528354806No Hit
CATAGGGGGTGTGGTCAAAGCAAAAGTTGCACTATGGTCTTGTATAGCCTAT2260.10391091248494211No Hit
GGGACCGGGATGGGCTCCAGAGTGTGTTTTAAAAAATACCCACTATTTCATT2260.10391091248494211No Hit
ATCAAGGGGGTTCTTTCATTAAAAGACCAAAAAGAAGTTAAAACTTAAATTG2240.10299134688773023No Hit
TGTGCTGGGGATTTCAAGGCACATTATTATCATCAGTCTGGGTGAGGTATCT2240.10299134688773023No Hit
ATGCACGGGAGAGAAGAGCCAAAAGGAGAAGAGAATGCTTTTATTTTTAATA2230.1025315640891243No Hit
ATCGCGGGGTGGCATTTCGAATGGAATAAATGGGGGCAATCTCTCATTTCTG2230.1025315640891243No Hit
CAGTTAGGGGAAGGAAATTAGAACATGCTTGTAAGCATCATGTTCAATTAGA2230.1025315640891243No Hit
CGCCATGGGGTATATTGAGGATAATCGTGTGTTCTCTGACTACTTCACAGGA2230.1025315640891243No Hit
TCATAGGGGGAACTGCGGCTGCTGTTTGGAGCTCGCGACGGAGCCGGCTCGG2210.10161199849191241No Hit
TAACAGGGGAGCATTAAAAAGTAATGCTTTATATGATCTTGTTTTATTACAA2200.10115221569330649No Hit
GACCTCGGGAACCTATTGATGTAAGTATTAAGAAGTTTTTCATCAACTTTAA2200.10115221569330649No Hit
GTGCCAGGGCATGCATTGATTATAAAAATTCAGTATTAATTTTTTCTGATCT2200.10115221569330649No Hit
ATACAAGGGATAAAGTGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGAC2190.10069243289470055No Hit
ATACTTGGGAAGCCATATTAGATTGCTTTGATCAGGCTGCCATTTGAGAAGC2190.10069243289470055No Hit
AATCACGGGATACTCACTCATTTAGTTGCCGTTTCTGGTGTTCCTCATTTCT2180.10023265009609461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)