FastQCFastQC Report
Sat 31 Dec 2022
SRR5849053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102538
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC16831.6413427217226784No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT15311.4931049952212838No Hit
TATCATGGGACGATTTTGACTACTCTGAGTATGACATGTAAATGGAATCATG3390.3306091400261366No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC2780.2711189997854454No Hit
CAGCATGGGGATCTTCTTTAGGGATGAAACCTCCCAAACATTTAAGGAAGAA2270.22138134155142483No Hit
GAAGTGGGGTTGGTAGTAAATTTTGAAATCAGCTAGTATAAGTTTTCCAACC2110.20577737034075172No Hit
ACAGCCGGGAACTTAGATCTAGGATGTATAGAACCTGACAAATGATATGAAT2040.19895063293608223No Hit
TCGTGCGGGAGAACGTATGCTGTTCCCATTGCCCCGGGGCAGTGCCCTCTCC1950.1901733991300786No Hit
ACATTTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1920.1872476545280774No Hit
CTCTCTGGGGAAAGCTTTTAGAAGGCTTTAGTTTTAGAGTATCAGGGAAGTG1870.18237141352474204No Hit
GGTCTAGGGATAGACTGTTATATATAGCATATTATTGTTGAAATACAAAACA1850.1804209171234079No Hit
GGCCAGGGGGATATTGGCTACTGTCAAATATTTAAAAGATGAACAATGTTTG1840.17944566892274086No Hit
GTAAACGGGACCTGACTGTTGTAGATGCAGCTTCACTAAGACGACAGATAAT1820.1774951725214067No Hit
TCAAAAGGGGGCTTTGAAAGGGATTACAGTATCACACAATGTCAAGCTAGAG1810.17651992432073962No Hit
GAAATTGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC1760.1716436833174043No Hit
ATGCAGGGTCCACATTAGTGAAGGAATGGCTTGATTGAAAATTTGATTTCAG1750.1706684351167372No Hit
GCGCATGGGGATCAGAGTGCTCTAGAATAATGGATGGTCTTGGATGATGGAT1740.16969318691607013No Hit
TATACAGGGTTGCTGGTACTACACATGTTTAGAAGCTGAGCAATTGGTCAGC1730.16871793871540305No Hit
TGGCCTGGGGCCTGAAAAATTAGCCAACGCTTGGGGGGAAATGAGCTTCACA1720.167742690514736No Hit
GATCTAGGGTGTGCAGATAAAATAAAAGAATGCAATGTTCTACAGTAGTCAT1710.16676744231406893No Hit
TAAAGGGGGGAGGTGTGTTTGTGTTTGAGGAGGGTACAAGGAACTGCTGCTT1710.16676744231406893No Hit
TCTATCGGGCTAAGCGCGGGGTATGTCAGTGTAACATGCAGATGTATATTGC1690.1648169459127348No Hit
CAATTAGGGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCCT1680.16384169771206772No Hit
ACTCTGGGGTTCAAAGCTGCTGGGACGTTAGAATTAGGCCATGATACTGGTC1680.16384169771206772No Hit
CTAATGGGGATTCAAGATGAATTTAAAAGTCAACCCTTCACACAGTTTCCCT1680.16384169771206772No Hit
CTACCAGGGACTCAGGCTGGAGTGCAGTGGCACAATCTTGGCTTACTCAGCT1680.16384169771206772No Hit
TTTGACGGGGTTTTGGTTGTTGCTGCTGGAAAGCAAGTTTATGTACATATTG1660.1618912013107336No Hit
GGCAGGGGATTTGTATATAAGTAATTGGCCTGTGATTGTTTCTTTTTTGGTT1640.15994070490939943No Hit
TTAGTTGGGATTTCTCATAATTTCCACATCAACTCCCATGATGATCATGCAG1630.15896545670873238No Hit
CGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAAC1620.1579902085080653No Hit
GCGCCTGGGAGGTTGCCATGAGCCTTTTCTCCTTCTATAAACCGTGTCAGCA1600.15603971210673115No Hit
GAATGTGGGAGTACAAACACCTTAGCACATCACTGCACACACAGTATTCTGA1600.15603971210673115No Hit
TCTTTGGGGATCCTGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCC1600.15603971210673115No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1580.15408921570539702No Hit
TGCACAGGGGGATTAGGAATGCTCAGCTTGTATATACAGTCATGCCTTGCTT1580.15408921570539702No Hit
TTTCCTGGGAGATTCTTTCAGGTACTCAATCAGGAAGCTGGAGGTGTTAGAC1570.15311396750472994No Hit
ATATTTGGGTCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA1560.15213871930406286No Hit
GACTGGGTCACTGTAATAAAATTTACAGAAGGAAAGATCCTGTGTACCCTTT1550.1511634711033958No Hit
CGTACGGGGATGTTTAGGTGGAGCAAATATTTCGAAGGTGTTTGTGAATATT1540.15018822290272876No Hit
GCATCGGGGAAGGTTACTGAGATTACAATTCTTCAAAGGTTAATGATAATGT1540.15018822290272876No Hit
CGTCCAGGGAAGGGTGTTCGGTTGCGTGCATGTGCGTTTTTAGCAACACATC1540.15018822290272876No Hit
CAGAAAGGGATTATAGTAGGAGACCATTTTGTATTACACACAAACATGTCTG1530.14921297470206168No Hit
ACAAACAGGGAGGGGTGTGTTTGCCACATGGAGCATCCCTTCCTGGTCTTGC1530.14921297470206168No Hit
ATTTAGGGTCTGTACATGGACCCTGCATAGTCCTGGATACTTGGGCCAACGT1530.14921297470206168No Hit
ATTGCTGGGATTCTAAATGATGTAGAAGGTTTAAAAATAATTACATTATGCT1520.1482377265013946No Hit
GTAAGAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT1520.1482377265013946No Hit
GTAATGGGAGTATTCCCAATTCCACATACACCCACACCCCAGAGCAATATTC1510.14726247830072753No Hit
GTATCAGGGAGATGTAGAACCATTTAATGAGATGAGCTATTCTGTGAGAGGA1510.14726247830072753No Hit
CTAGGGAGTGAACCAAATCTAGAAAGTACCAAGGCAGAGGTATGCTCCTGCT1500.14628723010006048No Hit
TGTAGTGGGTCCTGGAATAATAAAGTTTGATGTTCAGCAAGAGAACTGCTTG1500.14628723010006048No Hit
TAGTGCAGGGACAACCATATATTGAGGTTTATTGGGAAGATTCTCTATTGGC1490.1453119818993934No Hit
TCTCGAGGGGTAGGTTATGTGTACATTTTTTGCGTAGTGAAGTACTCTGTCC1490.1453119818993934No Hit
TAGGTTCGGGGAAACTACAAGCAATAAATTTTAGTAGGGGAAATGTGTACAT1490.1453119818993934No Hit
TATAGGGGGTGATGTTGGGAAGCTATTTCCTTTGCGCAAACTTTAACAGGTT1480.14433673369872632No Hit
ACTGCGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1480.14433673369872632No Hit
GACAATGGGGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGTA1480.14433673369872632No Hit
GCACAGCGGGCCTATTGTCCTTTATGCATTTTCAATGAAATGGAATGAAGGC1470.14336148549805924No Hit
GTAACTGGGGATCTAAGTTGGGGATGTGGCCCGATGGGGATGTGCTGTTACC1470.14336148549805924No Hit
GCTCCTGGGATATATAATACACTAGAAAAAGAAATACATTAAAAAAAGAAAA1460.1423862372973922No Hit
TAGTGAGGGCATCTGTTAGGAGCACCTTGACATTTTCAGTATGTTCAAGAAA1460.1423862372973922No Hit
TTCTATGGGGAACTTGTGAATTTGTGTACAAATCCTGTTTATGTTTCATTTG1450.14141098909672511No Hit
TGTAATGGGTAACAGCTGTGTGATCTTGGGGAAGTTACTTGGCCTCTCTGTG1450.14141098909672511No Hit
CACACAGGGGCTTCCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAA1450.14141098909672511No Hit
GGCACAGGGCAGAGGATGCTGCTCCGAACTGTTGGAAACAAGGACATTAGCT1430.13946049269539096No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA1420.1384852444947239No Hit
GCAATTGGGGAAGGTAGTAAATCAGGGTTCTAACAGTTGGAGAGTTTTTCCA1420.1384852444947239No Hit
CTAGCGGGGATTCCTCTGACAGCAGCGTCACTCCCTCTGGCAGCCCCTGGGT1420.1384852444947239No Hit
GGCTATGGGGTGAAGTCCAAATTCCAATGATGTATCAGCAAGGAGAATTTTA1420.1384852444947239No Hit
TTCACCGGGAATAGTTGAAATGTATATTTGTACAGTCTTTTAGACCTATTCA1420.1384852444947239No Hit
TTTGCGGGTCAAGTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCA1410.13750999629405683No Hit
GTTTTAGGGGATTCTGAGCCGAGCCCGGTGCCAAGCGCAGCTAGCTCAGCAG1410.13750999629405683No Hit
ACTGTTGGGTGTCTAGTGAAACTTATCTGATGGCATTTATTGAAAACCTTCT1400.13653474809338975No Hit
TAGCATTGGGCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACT1400.13653474809338975No Hit
CGTCATGGGAGAAGAGAACTCCAAATGAAACCACATCAGTTTTAGAACCAAA1390.1355594998927227No Hit
GATTGAGGGATCAAGAGGAAGCAGAACGTCTGCACAAGCGGGTGACTGAACT1390.1355594998927227No Hit
TTTCAGGGGATCTGTGCTTCTAATATTAGTTGGCTTTCATAAATCATGTTGT1390.1355594998927227No Hit
CGTAATGGGAGTTTACAGATATTTTTGACCAGTTCCTTTTAGAGATTCTTTC1380.13458425169205562No Hit
TGCCATGGGCAAAGGGGATATTACCACCGACCCCAGATAGATACAAAGATTT1370.13360900349138857No Hit
ACAGCAGGGCATCACTGGTGTCTGCTGATGTCAGAAGAACAGAAGGCATTTG1370.13360900349138857No Hit
GAACCTGGGGATTATAGAATGTTGGTAGCTAAATTGAAATAAAATTTAAATA1370.13360900349138857No Hit
CTGCCTGGGCTCTGAAATCTCTCTTAATTCCTGCTGTCCAGGCAGCAGAATG1370.13360900349138857No Hit
GGTATGGGATCCAATTTCTGTTTAGTCCATGATGAAAACACCCCATTTTTAT1360.1326337552907215No Hit
GCTTATGGGAACCACGGCCAATGTTCCTTAGCTGCACCAGGCCCGAAGCTGT1350.13165850709005442No Hit
CTTGCTGGGGACCCGTCCTCAATAACAATCCCCCAGCTGTGGTGGTGAACTC1350.13165850709005442No Hit
TAGGTAGGGGGAAGACAGCTACGGGCAGCAGTGGACCTATGAGCAGAGGAAA1340.13068325888938734No Hit
TAATGCAGGGACTAGACAAAGACTTTAGCTATTACAAATATAAATATGTTCA1340.13068325888938734No Hit
TTTCAAGGGGCCTCCCTCAGACAAGCAAAAACTGAGGGAATTCATTACTACT1340.13068325888938734No Hit
ACGTAGGGGCGATCTTCAGTAGGGAAATGAGGTACTGCATGTTCCTTTAAGA1330.1297080106887203No Hit
TACAGGGTTCCTAACATAATTGGAGAAACAGAGGAGGTAGAGCTTCAGGAAT1320.1287327624880532No Hit
CATGCAGGGGATTCCCACCCACGACTCACAGGCATTCCTCCCACAGCCCTTT1320.1287327624880532No Hit
ACAACTGGGGAGCAAAACTGGTTAGATTTTGCATGAAATGGTTCTGAAAGGT1310.12775751428738613No Hit
TTTGCTGGGGTTCTTTTTCAAGATTGTTTTGGCTACCTGGGGTTCCTTGAGA1310.12775751428738613No Hit
GTGAATGGGGTAATTGGCTTTTGAATCAAAGAATAGGGAGACAATCTAAAAA1300.12678226608671905No Hit
TTGTTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1300.12678226608671905No Hit
GCTCAAGGGGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCATCAT1300.12678226608671905No Hit
TATTACGGGGTGTTGGCTTGGCTGTTGACATGGATGAAATTGAAAAGTACCA1290.125807017886052No Hit
CAGTAAGGGATAATTTTATGTCTATGTTTAATCATTGCCAAAATTTTAATTT1270.12385652148471785No Hit
TTATGAGGGACAGATACTAATGTCAAGAGTTAAACCTCCTCAGGTTCAACCT1260.12288127328405078No Hit
TCTCAGGGGTACCAGCACCAACGGCAACAGATGCTGTGCCTGGAGCGGCATA1260.12288127328405078No Hit
CAGCAGGGGATGCTGCACCCACTTAATCTACTGTGGTGTTTTGTTTATTAGT1250.12190602508338373No Hit
TGATGTGGGAGCTGCAGGACTCTGGCAATAGGGTTTTTGGAAGATGTAATTT1250.12190602508338373No Hit
GAAGCTGGGAGAGATACAATCTTGCTTGACCGTTTGGGACATCAATCTTCCA1250.12190602508338373No Hit
AACGATGGGAACCATCTGCGAAAGGACACACACAGGAAATTCATAAAAGAAA1250.12190602508338373No Hit
ATGATAGGGGTATGCATATGAAATTCAACCAAGAAATGATTCCCACAGTTAG1250.12190602508338373No Hit
AACATTGGGGTTTCTCAAACATGCTGAAGTTCAGCATTCCCACCTTGGGGCC1250.12190602508338373No Hit
CCTGGCGGGATCTATGAAGGGTTGTGCAAAACAGGAAGGTCTGGGAATGACC1240.12093077688271667No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1240.12093077688271667No Hit
ATGATTGGGATGAGGTATTTACTGTGCAATAACTGATTCATTTTTTTCAGAG1230.11995552868204959No Hit
GCAAATGGGTTCGAATGGCGAGGATGGCAAGAAAAGCTGGCAACTTCTATGT1230.11995552868204959No Hit
TAAGCAGGGGATCCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAAC1220.11898028048138251No Hit
AAAAGGGGGACTCTTTAGCCTTGTTTTCACTTTATTAATATGAAGGTAGTGA1220.11898028048138251No Hit
TCTTTGTGGGGGACAGACCGTGTGTTTCCAAAATGGCGGCAGCGATGGATGT1210.11800503228071545No Hit
ACTTCCGGGCTAGGCGTTTACCGCACGCTCTATCTCTTCAACTGGATCTGGC1200.11702978408004838No Hit
TATCGAGGGACTTTTTCAATATCCTGAGTTTAGGATACTGAGGAAACACATG1200.11702978408004838No Hit
TACATAGGGATCACACCATCGCTGGCCCCTTTGGGCCCTGCATGTAGTGTGG1200.11702978408004838No Hit
GTTTATGGGATGTGCTGAAATAATTACTGGTAAAATTTCAGGGTTGTGGATT1200.11702978408004838No Hit
GGCACGGGGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT1190.1160545358793813No Hit
AGCTATGGGAACATATTTTGGTAGATATACTTATAAACCAGAAAATACATTT1180.11507928767871423No Hit
CCGCGATGGGTGCTATCGGGCATGGACAACAAGACCAATAAGAGGCGTGGGT1180.11507928767871423No Hit
AACAAGGGGGAACCCCAAGTTTAACTAATGGAGACAATATCAGGAGCAGGTG1180.11507928767871423No Hit
AATCCTGGGGGTGCAATCTCGGTTCACTGCAACCTCTGCCTGCTGGGTTCAA1180.11507928767871423No Hit
ATCCTAGGGATTTGCTGTGATTACTTAGGTTTAGACTTTCTCAACTTCTTAG1180.11507928767871423No Hit
TGTAATGGGACTAGTTTACTATGTATCAATTCTTTATACTCCAGTCATAAAT1170.11410403947804716No Hit
GATTGAGGGATGGAGCATATATTATGGAACACAGTGAGTATAGTACCTGCCT1170.11410403947804716No Hit
AGACAGGGAATCTCGTGTCTGCAAATCAGACTATTGGGTTTGGCAGTCCCTC1150.11215354307671302No Hit
TGCCTGGGGGACCATTGTGAAATCCTTTCTCAGTGTAACTGCATTTCTAATA1130.11020304667537888No Hit
TTTTAAGGGACCCCTTAAGCGTTTAAAATAAAATATGCAACAAAATGGATGA1120.10922779847471181No Hit
TTGCCTGGGAATATAATTTTCTCACAGATTTTTCTCCCACTTAATTCTCAGC1120.10922779847471181No Hit
CACACAGGGGAACACTTGGTGCTGGGAACCTCAGGGTATTGCTTGCCACTAA1120.10922779847471181No Hit
CTCTGAGGGACAGTTTCAGCCTAAAAAGAGCCAAGATCTCACCTATTATTAC1110.10825255027404473No Hit
TCAGCAGGATTGCCAACTGGAAAGTCAAAATTTTCTAACAACTTTAAGTAAG1110.10825255027404473No Hit
GTGCAGGGGGACCTGGGGTGTAAATGGTGAGACGGGTACTTTGGTGGACATG1110.10825255027404473No Hit
CTAGCAGGCAATGTTAAAATACATTTACTATAAAATACTAAATGAATAATAA1100.10727730207337768No Hit
CCTATGGGCTGGCCATTCCTTTCGGTGCTGCTGTCCTTTTTGGGGGGGTTCC1090.10630205387271062No Hit
GTCGCCGGGACCAGAAGCTGTCGGAGCTGGACGACCGTGCAGATGCACTCCA1090.10630205387271062No Hit
GCGCAGGGACAAAAACAAAACAAAACAAAACAAAAAAACATTCTACAGTGGC1090.10630205387271062No Hit
GTAACAGGGAGAGCTGGTGCCAGATGTGGGCTCAGATCCTGGGCATGATGGG1090.10630205387271062No Hit
TAAGTGGGGTGCCAATATTTTTCAAGGAGAGGCTTCTTGCTGAATTTTGATT1080.10532680567204354No Hit
CGGCCCGGGATGGGATGAGAGGAGAGAGGTTTTTTAGACAACATTTGTAGAC1080.10532680567204354No Hit
CTGCCTAGGGGATTGAAGCATGTAAATTTCAAAGAATAGCCTTTTTTTGTGC1080.10532680567204354No Hit
GTGTAAGGGAAAATGTATAAATGATGGATAGATTTTTGTATTGATTTGCAAA1080.10532680567204354No Hit
GTGGTAGCATGGGGGATAAAAAAGAATAGAAATGGAAAGTGGGACTGAGAGG1080.10532680567204354No Hit
TCCTCGGGGGAATTGACACATAATGAAGGCTCAGTAAACATTCATTTTTGTT1070.10435155747137648No Hit
CACTTAGGGAGGTTTTTTGAGGTCTTAAGCTAATATAAATACAAAATACAAA1060.1033763092707094No Hit
GGCGCTGGGGAGATTTATTTTGATGGACGTGATATAAAGAATGGTTGTTGCG1060.1033763092707094No Hit
CTAAAAGGGGAAAGCACGGACCACAGTTTCAATGTTGTATCAGTAGTTTCCT1060.1033763092707094No Hit
CTAAGAAGGGGATGGAACCAAAAAAGAGCCTACATAGCCAAAGGAAGACTAA1060.1033763092707094No Hit
GAAGCGCGGGGGAAGATCAATTGTATCATTGGTAAAATAGGTTATATTACAA1050.10240106107004233No Hit
TAATCTGGGCGATCTGATTATGGGCCGATCTGGAACGGGACAGCAAGAAGAT1050.10240106107004233No Hit
TGCACAGGGAGACTCATCTTACTGTACCTGTTCCTTAGACCTTCCATAATGC1050.10240106107004233No Hit
ATTATGGGGGTGGCTGAAGAGAGTGAGAGGAACTGGTTGGGTGGTCTCTGGC1050.10240106107004233No Hit
GGTCGAGGGCTGTCCCGAGCAGAGGTAATTCCTCACAACTTAGTGCACCAGT1040.10142581286937526No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC1040.10142581286937526No Hit
CTTGTGGGGAGATGGTGTTACCTGGAGCTTAAAAAGCTGCACGCAAGTGTTA1030.10045056466870819No Hit
AGCTAGGGGACCTCAAAGGGGTGGTATAAGGTTTAAAAAGATAAAGATTCAG1030.10045056466870819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)