FastQCFastQC Report
Sat 31 Dec 2022
SRR5849048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167307
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT28511.7040530282654045No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC21201.2671316800851131No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC3190.1906674556354486No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT2520.15062131291577757No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC2500.14942590567041425No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTC1860.11117287381878821No Hit
TGACTAGGTTATGCATTATATTTGACTGTTGACTCTTTAGTCACTCAGTGTA1740.10400043034660834No Hit
CACAGCGGGACACTAACTGTAGAATATCCACACAATAAAACTATTTTTTTAA1690.10101191223320005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)