FastQCFastQC Report
Sat 31 Dec 2022
SRR5849047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165523
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC27731.67529588033083No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT24151.4590117385499295No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3410.20601366577454494No Hit
ACACAAGGGGATCAACATTTATTGCATGCTTATTACATGCCAGTCACTCAAC3030.18305613117210298No Hit
CCGCCTGGGAGCTCCTTTCACTTAACTGAAAGTCCAATGCAACCTCTGATAA3030.18305613117210298No Hit
CATACTGGGCAGGTTGCTTGAGCCCAGTAGTTTGAGACCAGCCTGGCAATGT2770.1673483443388532No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2660.16070274221709369No Hit
GGCAATGGGAGTTGCAAGTGTTGAACAAGTTACTCTTTTGAAGCTGATGTGC2530.1528488488004688No Hit
TACGACGGGGCTATACTATTCGTTTGTTCTTATTAAGCCTTATTGTGTGTAA2480.14982812056330538No Hit
CAAGATGGGGATCTAAGATGGGGAGCTCACCAACACCTACTGTCTGGTAGCG2460.14861982926844003No Hit
GTCTTTGGGATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGA2430.14680739232614198No Hit
TTAGCGGATTGCACTAAATTTGTTGAACCTGTGGTAGGCACATCTCTTAGGA2350.14197422714668054No Hit
GAGCAGGGGGCTACTTGACAGTTTATAAAATAGTTATAAGCCCAGATTTCTC2340.14137008149924785No Hit
ATACGAGGGGAGATCCTAGAGGGAGTCAGGCTGAAGAGGCGGGACTTTCCTG2330.14076593585181515No Hit
CCTATTGGGGAGAAACAAAGACTTCCAAGAGGTCACCCTTGAGAAGAACGGA2310.1395576445569498No Hit
AAATTGGGAAGAAGCTGCTATGAGGTCTCGTAAACAGGGTCTTGACAATGTT2310.1395576445569498No Hit
CAGTAAGGGCAAAACAATATTACTTTGTCTTTTCAATCTTCTACTTTTAACT2310.1395576445569498No Hit
CTAGGGAATGAAATCACTTCATAAGGTGTCAAAAGGTATTCAAATGTTACGT2300.1389534989095171No Hit
CAATACGGGTACATTTTTGTATCATAATAGATTTTAAAAATCTAATGTTCTT2290.13834935326208442No Hit
TCAACAGGGAGGGTTTGCGCTGCTATGAGAATCTAAGGCTGCCACTGATCTG2270.13714106196721906No Hit
GTAGAGGGGGTCACCCTGAAGCCCTTAGATTCCAACCCCTCAATCAGAGACT2260.13653691631978637No Hit
TTATCTGGGATTCCAAGAATTAAAATATAGAAATGGGTATATCAACCCAAAT2250.13593277067235368No Hit
GGTAATGGGGCAAAGAACCTAAATAGACATTCTCAAAATAAGACATAAAAAT2230.13472447937748833No Hit
GAGATAGGGGGAAGCAGTGAGAGCTGGGAAGCTTCTTTTGGCTCTAGGTGAG2220.13412033373005564No Hit
TCATAAGGGAGAAAACAGGAAGAGAAATATGCATAAACCAGGGCAGCTCATT2200.13291204243519028No Hit
TGATATGGGGCGGAATGTCTGGATTAAGAAGCATTACCAATAGGAATGGATC2200.13291204243519028No Hit
TGTCAGGGATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAAC2200.13291204243519028No Hit
GAAAATGGGGAAACAAACATTATTGTTGAGAGTTTGTTTATCCTATGGTGCT2190.1323078967877576No Hit
CAGAGGGGCCCTTATACCTCTCGGATCTTGAAACCTTATATCAGGCGTGATT2180.1317037511403249No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC2170.13109960549289223No Hit
TGATAAGGGGGGATTTCATTTTGGTTCCATCAAATTAGGAAAAGAAATGGCT2130.1286830229031615No Hit
CAAGCTGGGACTGTGAGAGTAGGACCCAGATCCACCTTGCTCACTTCTGTGT2130.1286830229031615No Hit
AGTACGGGACGACATGTATAGGTTACCTAGATGTTATGAGAAATTTTAGTTT2130.1286830229031615No Hit
TAATGGGGGATAAGATCTATTAAGCTTGACACATCTGTGTCATCACGCACTG2120.1280788772557288No Hit
GATCATGGGGAAGCTGCCAGCCTATAAGGCACAGTAGGATGGTGTGGACTAT2110.12747473160829614No Hit
AAATCAGGGTGTGTGACTATATTCCATAGAGAAGAAAGATGGGATAGCTCAA2100.12687058596086345No Hit
GGTATGGGGCCGGCTGCCGCATCATCGGGCGCATGTGTGTGGGGAACAGGCA2100.12687058596086345No Hit
TAAGATGGGGATGAGGTTCTAGGACATTTACAAAATTCCATGTAACCTGTGT2100.12687058596086345No Hit
GGACGAAGGGATTACATGTTCTAAATGCTTTACTTGTCGTATTTAATCCTAA2090.12626644031343076No Hit
ATTGCTGGGGTAAATGATGTCTGTAATGGGTTGAGTTACTGAGATGACAATC2090.12626644031343076No Hit
GTTTGGGATACCAAATATTGGGAGGACCTGGTGAAAGATGCTTTTAGGATCT2090.12626644031343076No Hit
TGGCTGGGGGGGAGGAGGAAGGTGGAGAATAGCAGATTGTGGGTGATATAAT2070.1250581490185654No Hit
ATTTTTGGGCAAGGCGGGGCCTCCACACCACGAGTCCCATGCCAAGGTGATG2070.1250581490185654No Hit
TATACTGGGGTCTAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCG2050.12384985772370004No Hit
TAACGTGGGAAACGTTTAACGTATAATGTCTGTTTGGATACTGTTCCAAATT2050.12384985772370004No Hit
TTGTTAGGGGTGGAAGTGGCTGGCCTGACATCCTTCTCCTTTGGGGAAGATG2040.12324571207626735No Hit
GCAACGGGGGCTCCAGCGCTGACGCCGAGCCATGGCGGACGAGGAGCTTGAG2030.12264156642883466No Hit
GGCGCAGGGAATCCTTTTTAGAAAACTGTTTTGTCACATCTGTGAGACAATG2030.12264156642883466No Hit
AGCGCAGGGATTTTAGCTTCACTTCTGACTGAACAGTCATTAGTCAACTTTT2030.12264156642883466No Hit
TCATGTGGGACATGTTTCTAGACAGCTGTTAGATGCTTTAAATTCCAAATAG2020.12203742078140199No Hit
CTGTGGCGGGCGATCTAAAAGTGGGCACTGGACAGACCACCCACTGCCGCCG2020.12203742078140199No Hit
TGTCAGTGGGATAGCTGTGGTTATGTTATTTTTTATTTAAAAACTTTATATT2020.12203742078140199No Hit
CAAAATGGGCACATCATAGAAAGCATACAAGTCACAAATAAGGAGATAAATT2010.1214332751339693No Hit
GGATTGGGACAAGAAGCTTAAGAAGAAGGATAAAGGGAAGCTGGAAGAGAAG2000.12082912948653661No Hit
CAATCGGGGCTTCTAGGAACTGCCTGGTTTTCAAGCAGGTCCTGGCTGAGCG2000.12082912948653661No Hit
TAGAACAGGGAGTATCATACGTTGATAGTTTACAAACAAGATACGTATATTT2000.12082912948653661No Hit
CACTTTGGGTCTTTTGCCGGGACAGTGTTTTTTAAAAAGACACAAAAAAACT1990.12022498383910393No Hit
GTATGGGGTCAGGGTTTAGCAGAGAAACAGAACCAATAGAATCAATCTATCT1990.12022498383910393No Hit
TTTTGCGGGAATGTCATAATGGCCTCCAGGGCAGTGAGATTTGAAAGCCCTC1970.11901669254423856No Hit
TTAAGGGGGAACACATGGTTAGAAGGGATAATTTATACTTCAATTTTGAGGA1960.11841254689680589No Hit
TGCTCAGGGATCTTGATTTCCTTTTCACATCAATACTCTAAGATCACTATTT1950.1178084012493732No Hit
GGCACAGGGACATTTGAATATGATTTGATGTGCTGTTTGATAAAAGAACATC1950.1178084012493732No Hit
CCATCAGGGCCATGAAGTCCTAAATCTTCTTTAAAGTCAGAATAGGTATCTT1940.1172042556019405No Hit
ATCTAAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA1930.11660010995450784No Hit
TGCACTGGGAGCTCTCAGAAGCGCTACCGGGCCGCCAGTGCCTTCTTCACCT1930.11660010995450784No Hit
AGACTAGGGGATTTATTGTACTATAGATGGTGAAGCTTTATTCAAGTATCAA1920.11599596430707515No Hit
TACTGCGGGCAAACAGCTCGATAAGCCTTTTTGACTGTGTACATCTGTACCG1920.11599596430707515No Hit
GTAGAGGGGATTAGCCATGTGATCAGGGGCAAATCATGTAATCATCTGTTTT1920.11599596430707515No Hit
GTGTTTGGGAGTCCTGATGTGGCTCCTGACCTACGTTGGCGCTCTCTTCAAT1910.11539181865964246No Hit
CACTGGGGACCAGGGGTTAGAAGTTGCCTCATTGGTTTAGTTCCTTGAGTGT1910.11539181865964246No Hit
CTTAGTGCGGGACCTCACAACAACCATTTGCCTCCTTTAAGAACACTGATGG1910.11539181865964246No Hit
ATTGTGGGGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGTGCTC1900.1147876730122098No Hit
ATCTTTGGGAGGGTATTTACAAGAAGACAAGTGGAAGAATTGGGGATCTTTG1900.1147876730122098No Hit
TGTTTGGGGACACGACCCCAGTAGGTAACCCTGAGTGCTCAGGTCTGAGCTG1890.1141835273647771No Hit
TAAACTGGGATCCTTTCTCATGGAAGCGCGTACCCGTTCGTGGAGGAAACGG1890.1141835273647771No Hit
AATACTGGGAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTGT1890.1141835273647771No Hit
GTATATGGGGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAAT1880.11357938171734441No Hit
CTGATGAGGTCCAGAAGCAATTTTATAACAGATTTCATTTTAACAAAATACT1880.11357938171734441No Hit
CCGAACGGGATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAAT1870.11297523606991174No Hit
TAAGCAGGGGTAATAAAACTTGGTTGGCTTGATATTTTAAGGAATTGAAACC1870.11297523606991174No Hit
CAAGTAGGGAGGAGAGGTAGGTAAATAAGAAAGATCCTGTGACTTAGGATGC1870.11297523606991174No Hit
TTGTTGGGGATGGGGCAAAATGAGAGGGCTTCACTTTGTCCCTCACCTGCCC1870.11297523606991174No Hit
ACATGGGGGTACCTGTAAGAATGATTACACAGACATGATAAAAGCGCTTTTA1860.11237109042247906No Hit
AAGACCGGGATTCTACTTCACATCAACAGAACATATATTTTTTCTCATCTGT1860.11237109042247906No Hit
GCTCGGGATGGACCCAAACTAGACTATACTTGGATAAGTTAAGCTCTTCTTC1850.11176694477504637No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1840.11116279912761369No Hit
GGAAATGGGATGTCCGAGGTTGGACAGGCAGTAGCTGGACAGATGCCATTGC1830.110558653480181No Hit
GCCATTGGGGTGCATGTTTTTTTGACTGGAAAGTGGAAAATATTGAAATGTG1820.10995450783274831No Hit
ATGAATGGGATGCCAATCACTGAGACTGCATCAGAGTTTGCAATTTTGTATG1810.10935036218531564No Hit
ATGGGTGGGGATCATTTCCAATCCACTGTTTAAATGCAGAAATAAGTAGCAG1800.10874621653788295No Hit
GGGTACGGGGCTTTTAAGGTGTTTGTTTAGGGATACAATGACCACTAGATGT1790.10814207089045026No Hit
CACATAGGGGTTTGGATTTTCTGTATACATTGAGGAAGCATTACCGGAATGA1790.10814207089045026No Hit
ACACGAGGGCCTCATCTCTGAGATGAGTAAAATCTATGTGTGTTGCTATGGG1780.1075379252430176No Hit
ATATTTGGGTGTGGTACAATATTTTCAGTGCAAAAGAGATGTCATTCAGTTA1770.10693377959558491No Hit
TAGCAGGGACCAGAGGAATCTGACACCTCTGACATAGATAGATAGATAGATA1760.10632963394815222No Hit
ATCCTTGGGGAAGCCAAGTCCCAGGTGTCCTGGCTGCTCTCCTGGGCGGGGT1740.10512134265328685No Hit
TTTTATGGGAAATGGAAACACTATGTAATGAAGGTTACTTCTCTATAAAATG1740.10512134265328685No Hit
AACAAGGGGGGAATTTTCAAACTTACAGTGTAATGAAACCTATATATCCATA1730.10451719700585417No Hit
GTAGACGGGAGGAATTTTGATTGCATTAATTTGTGGTTTAGTGAAGGGAGAA1730.10451719700585417No Hit
ATTATGGGGATGTTTAAAGCATTAATAAAATGTGATGAAGTACTTGCACCTG1730.10451719700585417No Hit
CACGCTGGGACTGCAGCATGAACTGCAAGCTCCCCTCAGCCCATCTTGCTCC1730.10451719700585417No Hit
GCATGTGGGATAGCAATCTGATAGACATGATGAAGGCAACATCTAAGGGAGA1730.10451719700585417No Hit
TATTTTGGGAACCTTATTTGCTTTATTAACTCTTATTTATCTTTCTCTTTTC1730.10451719700585417No Hit
TAGACTGGGATCTCCCCAGCGGGCAGGCGGGCGGAGCCTGTATATATGTATA1710.1033089057109888No Hit
ACAGTGGGGTAGCAAGACTCTGTGGAGTTTGTTCAGTGGTACGGTGTCCAAG1710.1033089057109888No Hit
AGCCTGGGCGACAGAGAGAGACTCCGACTCAAAAAAAATAAATAAATAAAAA1700.10270476006355611No Hit
TCCCAAGGGCATTCCTTAGACAGCAGTGGTCTTTATCCCTTTCTGGAAAGAA1700.10270476006355611No Hit
TAAGCACGGGATTTTTCTAATTCTAACAAGTTAGAATGTGAGGAAGGAATGA1700.10270476006355611No Hit
GCACTCGGGATTTTTGTATATAACTGGTTAATTTTAATAAGAGATACCATTA1700.10270476006355611No Hit
TGCCTTGGGAGGAGTGGCTCAGTGTTGGGGATGGACGCTGTCGCCCAGCCAT1690.10210061441612345No Hit
GTAAAAGGGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT1670.10089232312125807No Hit
CCAACAGGGACTCAAGAGAAGTGAAAACATGTCTGTGAAAAGATTTGTTCAT1660.1002881774738254No Hit
ATGATGGGGATCTGGTTCTTGGGATTTTTTTTCATAATTAAAATTATGATAT1660.1002881774738254No Hit
ATCCACGGGGATTTTTATTTAAATAAATATTATGATTGCATTTTGTATATAA1660.1002881774738254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)