FastQCFastQC Report
Sat 31 Dec 2022
SRR5849046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164663
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC27751.6852601981015771No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT24401.4818143723848103No Hit
ACACAAGGGGATCAACATTTATTGCATGCTTATTACATGCCAGTCACTCAAC3510.2131626412733887No Hit
CCGCCTGGGAGCTCCTTTCACTTAACTGAAAGTCCAATGCAACCTCTGATAA3290.1998020198830338No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3140.190692505298701No Hit
GGCAATGGGAGTTGCAAGTGTTGAACAAGTTACTCTTTTGAAGCTGATGTGC2650.16093475765654702No Hit
TTAGCGGATTGCACTAAATTTGTTGAACCTGTGGTAGGCACATCTCTTAGGA2640.16032745668425816No Hit
AAATTGGGAAGAAGCTGCTATGAGGTCTCGTAAACAGGGTCTTGACAATGTT2640.16032745668425816No Hit
CATACTGGGCAGGTTGCTTGAGCCCAGTAGTTTGAGACCAGCCTGGCAATGT2620.15911285473968043No Hit
TTGTTAGGGGTGGAAGTGGCTGGCCTGACATCCTTCTCCTTTGGGGAAGATG2600.15789825279510272No Hit
CAAGATGGGGATCTAAGATGGGGAGCTCACCAACACCTACTGTCTGGTAGCG2600.15789825279510272No Hit
ATTTTTGGGCAAGGCGGGGCCTCCACACCACGAGTCCCATGCCAAGGTGATG2600.15789825279510272No Hit
CAAGTAGGGAGGAGAGGTAGGTAAATAAGAAAGATCCTGTGACTTAGGATGC2560.1554690489059473No Hit
CAATACGGGTACATTTTTGTATCATAATAGATTTTAAAAATCTAATGTTCTT2480.15061064112763645No Hit
GTCTTTGGGATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGA2460.14939603918305874No Hit
CAAGCTGGGACTGTGAGAGTAGGACCCAGATCCACCTTGCTCACTTCTGTGT2450.14878873821076988No Hit
GAGCAGGGGGCTACTTGACAGTTTATAAAATAGTTATAAGCCCAGATTTCTC2430.14757413626619215No Hit
CTAGGGAATGAAATCACTTCATAAGGTGTCAAAAGGTATTCAAATGTTACGT2430.14757413626619215No Hit
GTTTGGGATACCAAATATTGGGAGGACCTGGTGAAAGATGCTTTTAGGATCT2380.14453763140474787No Hit
TACGACGGGGCTATACTATTCGTTTGTTCTTATTAAGCCTTATTGTGTGTAA2380.14453763140474787No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2340.14210842751559244No Hit
CCTATTGGGGAGAAACAAAGACTTCCAAGAGGTCACCCTTGAGAAGAACGGA2340.14210842751559244No Hit
CAGAGGGGCCCTTATACCTCTCGGATCTTGAAACCTTATATCAGGCGTGATT2340.14210842751559244No Hit
CAGTAAGGGCAAAACAATATTACTTTGTCTTTTCAATCTTCTACTTTTAACT2310.1402865245987259No Hit
CACTGGGGACCAGGGGTTAGAAGTTGCCTCATTGGTTTAGTTCCTTGAGTGT2300.13967922362643703No Hit
TGCACTGGGAGCTCTCAGAAGCGCTACCGGGCCGCCAGTGCCTTCTTCACCT2300.13967922362643703No Hit
GGAAATGGGATGTCCGAGGTTGGACAGGCAGTAGCTGGACAGATGCCATTGC2300.13967922362643703No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC2290.13907192265414817No Hit
CACTTTGGGTCTTTTGCCGGGACAGTGTTTTTTAAAAAGACACAAAAAAACT2280.1384646216818593No Hit
TATACTGGGGTCTAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCG2270.13785732070957046No Hit
CTGATGAGGTCCAGAAGCAATTTTATAACAGATTTCATTTTAACAAAATACT2270.13785732070957046No Hit
GAAAATGGGGAAACAAACATTATTGTTGAGAGTTTGTTTATCCTATGGTGCT2240.1360354177927039No Hit
GTAGAGGGGGTCACCCTGAAGCCCTTAGATTCCAACCCCTCAATCAGAGACT2240.1360354177927039No Hit
GGACGAAGGGATTACATGTTCTAAATGCTTTACTTGTCGTATTTAATCCTAA2220.1348208158481262No Hit
TAATGGGGGATAAGATCTATTAAGCTTGACACATCTGTGTCATCACGCACTG2220.1348208158481262No Hit
TCATAAGGGAGAAAACAGGAAGAGAAATATGCATAAACCAGGGCAGCTCATT2210.13421351487583733No Hit
TGATATGGGGCGGAATGTCTGGATTAAGAAGCATTACCAATAGGAATGGATC2210.13421351487583733No Hit
ATTGCTGGGGTAAATGATGTCTGTAATGGGTTGAGTTACTGAGATGACAATC2200.13360621390354846No Hit
GGTATGGGGCCGGCTGCCGCATCATCGGGCGCATGTGTGTGGGGAACAGGCA2180.13239161195897076No Hit
ATACGAGGGGAGATCCTAGAGGGAGTCAGGCTGAAGAGGCGGGACTTTCCTG2170.1317843109866819No Hit
TCAACAGGGAGGGTTTGCGCTGCTATGAGAATCTAAGGCTGCCACTGATCTG2160.13117701001439303No Hit
AGCGCAGGGATTTTAGCTTCACTTCTGACTGAACAGTCATTAGTCAACTTTT2160.13117701001439303No Hit
TTATCTGGGATTCCAAGAATTAAAATATAGAAATGGGTATATCAACCCAAAT2150.13056970904210416No Hit
GAGATAGGGGGAAGCAGTGAGAGCTGGGAAGCTTCTTTTGGCTCTAGGTGAG2140.12996240806981532No Hit
GGTAATGGGGCAAAGAACCTAAATAGACATTCTCAAAATAAGACATAAAAAT2140.12996240806981532No Hit
CCATCAGGGCCATGAAGTCCTAAATCTTCTTTAAAGTCAGAATAGGTATCTT2130.12935510709752648No Hit
ATCTTTGGGAGGGTATTTACAAGAAGACAAGTGGAAGAATTGGGGATCTTTG2120.12874780612523762No Hit
AGTACGGGACGACATGTATAGGTTACCTAGATGTTATGAGAAATTTTAGTTT2120.12874780612523762No Hit
GCACTCGGGATTTTTGTATATAACTGGTTAATTTTAATAAGAGATACCATTA2100.1275332041806599No Hit
TGTCAGGGATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAAC2100.1275332041806599No Hit
TAAACTGGGATCCTTTCTCATGGAAGCGCGTACCCGTTCGTGGAGGAAACGG2080.12631860223608218No Hit
TTTTATGGGAAATGGAAACACTATGTAATGAAGGTTACTTCTCTATAAAATG2070.12571130126379332No Hit
GATCATGGGGAAGCTGCCAGCCTATAAGGCACAGTAGGATGGTGTGGACTAT2050.1244966993192156No Hit
AATACTGGGAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTGT2050.1244966993192156No Hit
TCATGTGGGACATGTTTCTAGACAGCTGTTAGATGCTTTAAATTCCAAATAG2030.1232820973746379No Hit
TAACGTGGGAAACGTTTAACGTATAATGTCTGTTTGGATACTGTTCCAAATT2030.1232820973746379No Hit
GTATGGGGTCAGGGTTTAGCAGAGAAACAGAACCAATAGAATCAATCTATCT2030.1232820973746379No Hit
TTTTGCGGGAATGTCATAATGGCCTCCAGGGCAGTGAGATTTGAAAGCCCTC2030.1232820973746379No Hit
TTAAGGGGGAACACATGGTTAGAAGGGATAATTTATACTTCAATTTTGAGGA2020.12267479640234905No Hit
ATATTTGGGTGTGGTACAATATTTTCAGTGCAAAAGAGATGTCATTCAGTTA2000.12146019445777131No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1980.12024559251319361No Hit
AAGACCGGGATTCTACTTCACATCAACAGAACATATATTTTTTCTCATCTGT1980.12024559251319361No Hit
ATGAATGGGATGCCAATCACTGAGACTGCATCAGAGTTTGCAATTTTGTATG1970.11963829154090476No Hit
GGCGCAGGGAATCCTTTTTAGAAAACTGTTTTGTCACATCTGTGAGACAATG1970.11963829154090476No Hit
TGATAAGGGGGGATTTCATTTTGGTTCCATCAAATTAGGAAAAGAAATGGCT1970.11963829154090476No Hit
TAAGATGGGGATGAGGTTCTAGGACATTTACAAAATTCCATGTAACCTGTGT1970.11963829154090476No Hit
GGCACAGGGACATTTGAATATGATTTGATGTGCTGTTTGATAAAAGAACATC1970.11963829154090476No Hit
GCAACGGGGGCTCCAGCGCTGACGCCGAGCCATGGCGGACGAGGAGCTTGAG1960.1190309905686159No Hit
ATAGCTGGGGAGCCTTCAGATGACTGTGGTCCCATCCCTTATCTGATGGCAA1960.1190309905686159No Hit
GGATTGGGACAAGAAGCTTAAGAAGAAGGATAAAGGGAAGCTGGAAGAGAAG1940.11781638862403819No Hit
AAATCAGGGTGTGTGACTATATTCCATAGAGAAGAAAGATGGGATAGCTCAA1930.11720908765174934No Hit
TTTAACGGGAAGGAATAGTAGGGGGAAAGTTAATGTGAGCAAGATTTTTACT1930.11720908765174934No Hit
GTGTTTGGGAGTCCTGATGTGGCTCCTGACCTACGTTGGCGCTCTCTTCAAT1920.11660178667946047No Hit
CAATCGGGGCTTCTAGGAACTGCCTGGTTTTCAAGCAGGTCCTGGCTGAGCG1920.11660178667946047No Hit
GGGTACGGGGCTTTTAAGGTGTTTGTTTAGGGATACAATGACCACTAGATGT1910.11599448570717161No Hit
ATCTAAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA1890.1147798837625939No Hit
GTATATGGGGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAAT1890.1147798837625939No Hit
TTGTTGGGGATGGGGCAAAATGAGAGGGCTTCACTTTGTCCCTCACCTGCCC1890.1147798837625939No Hit
AGACTAGGGGATTTATTGTACTATAGATGGTGAAGCTTTATTCAAGTATCAA1880.11417258279030505No Hit
ACCACAGGGACGACAGTTTTCCGGCCTAGTTTGCGGCTGCTCCTGCCGTGTT1880.11417258279030505No Hit
CTTAGTGCGGGACCTCACAACAACCATTTGCCTCCTTTAAGAACACTGATGG1870.11356528181801619No Hit
ATTGTGGGGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGTGCTC1860.11295798084572732No Hit
AATATCGGGCATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCT1860.11295798084572732No Hit
TGTCAGTGGGATAGCTGTGGTTATGTTATTTTTTATTTAAAAACTTTATATT1860.11295798084572732No Hit
AACAAGGGGGGAATTTTCAAACTTACAGTGTAATGAAACCTATATATCCATA1840.11174337890114963No Hit
GTTCCTGGGCACACACACACACAAAACAACAGCAACAACAACAGAACAACAA1830.11113607792886077No Hit
AGCCTGGGATGCTCAGTTTCTTATTAGTTTCATTGCTTAATTCCTGCTTAAG1830.11113607792886077No Hit
ACACGAGGGCCTCATCTCTGAGATGAGTAAAATCTATGTGTGTTGCTATGGG1830.11113607792886077No Hit
GTAGACGGGAGGAATTTTGATTGCATTAATTTGTGGTTTAGTGAAGGGAGAA1830.11113607792886077No Hit
CTGTGGCGGGCGATCTAAAAGTGGGCACTGGACAGACCACCCACTGCCGCCG1820.1105287769565719No Hit
TAGAACAGGGAGTATCATACGTTGATAGTTTACAAACAAGATACGTATATTT1820.1105287769565719No Hit
TGGCTGGGGGGGAGGAGGAAGGTGGAGAATAGCAGATTGTGGGTGATATAAT1810.10992147598428303No Hit
ATTATGGGGATGTTTAAAGCATTAATAAAATGTGATGAAGTACTTGCACCTG1800.1093141750119942No Hit
TCGAGAGGGGGAAAAAGTGAGTGCAGTGGTTAAAATGAGATGATTAATGCAT1790.10870687403970535No Hit
ACAGCAGGGGGTTTGTATTGAAATAAAAGATATTTTCTTGTAGCAGGGCTAA1790.10870687403970535No Hit
ACCAGTGGGGCATGGGAAAGCAAAGCAGAAAACAGTGCCCCAAATGGAAAAA1790.10870687403970535No Hit
TGCCTTGGGAGGAGTGGCTCAGTGTTGGGGATGGACGCTGTCGCCCAGCCAT1780.10809957306741648No Hit
ACAGTAGGGACATTAATACCAGTATCACTTAAATAGAAGGTAAAAACAAAAA1770.10749227209512761No Hit
ATGGGTGGGGATCATTTCCAATCCACTGTTTAAATGCAGAAATAAGTAGCAG1770.10749227209512761No Hit
TGTTTGGGGACACGACCCCAGTAGGTAACCCTGAGTGCTCAGGTCTGAGCTG1730.1050630682059722No Hit
GCATGTGGGATAGCAATCTGATAGACATGATGAAGGCAACATCTAAGGGAGA1730.1050630682059722No Hit
CCGGGTGGGTCTGACTTGGGGGGGGCCTATATCAGAATCATAGTTCCAGATC1720.10445576723368333No Hit
AAGACTGGGAGCAAAACATAATGAAGATATTTGTTAAAAACCTGTGACAACG1720.10445576723368333No Hit
TCGAAGGGGAATTGGTTGACTTAATATATTTGCTTTTTTTCTCACCTGCACT1710.10384846626139449No Hit
CCGAACGGGATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAAT1700.10324116528910564No Hit
CAAAATGGGCACATCATAGAAAGCATACAAGTCACAAATAAGGAGATAAATT1700.10324116528910564No Hit
TGCAGAGGGGAGGCAGGAGAATTGCTTGAACCTCGTGGGTGGAGGTTGCAGT1700.10324116528910564No Hit
GCTCGGGATGGACCCAAACTAGACTATACTTGGATAAGTTAAGCTCTTCTTC1700.10324116528910564No Hit
TACCCAGGGATGTCAAAGAGCCAGAAAGGCGGGGTCCTCAGAAACGACTGGC1690.10263386431681677No Hit
ACTTCGGGGCTTAGGCAACAGAAGGGAAAGCACTGCTGTGAAGGTGTCACTT1690.10263386431681677No Hit
CACATAGGGGTTTGGATTTTCTGTATACATTGAGGAAGCATTACCGGAATGA1690.10263386431681677No Hit
TTTCTGGGATTACTGGTGATAAAGAGGTTCATTTTATAATCATGAAGGTTTG1680.1020265633445279No Hit
TAACTGGGGGTAGTCTATATAAGTGATGTATGAGTTGAGTTATGTAAATTTT1680.1020265633445279No Hit
AGCCTGGGCGACAGAGAGAGACTCCGACTCAAAAAAAATAAATAAATAAAAA1670.10141926237223906No Hit
ATCCTTGGGGAAGCCAAGTCCCAGGTGTCCTGGCTGCTCTCCTGGGCGGGGT1670.10141926237223906No Hit
TGCTCAGGGATCTTGATTTCCTTTTCACATCAATACTCTAAGATCACTATTT1670.10141926237223906No Hit
TATTTTGGGAACCTTATTTGCTTTATTAACTCTTATTTATCTTTCTCTTTTC1670.10141926237223906No Hit
TAGACTGGGATCTCCCCAGCGGGCAGGCGGGCGGAGCCTGTATATATGTATA1660.1008119613999502No Hit
TCAAAAGGGACTGACACAGGCCTGGTTCATGCATAGAATGTCAAGTGGGAAA1660.1008119613999502No Hit
GATGCAGGGATTCATTATCAGCGCCAATGAAGCAAAGACCCCTGGCAAAGCA1660.1008119613999502No Hit
TGTGGTGGGCTTTTTGGCCTCCCCCTATTTCCTAGGATCCGCATTCGGGTGG1650.10020466042766135No Hit
CACGCTGGGACTGCAGCATGAACTGCAAGCTCCCCTCAGCCCATCTTGCTCC1650.10020466042766135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)