FastQCFastQC Report
Sat 31 Dec 2022
SRR5849045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165776
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC26981.627497345816041No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT23061.3910336840073352No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3620.21836695299681497No Hit
ACACAAGGGGATCAACATTTATTGCATGCTTATTACATGCCAGTCACTCAAC3010.18157031174597046No Hit
CATACTGGGCAGGTTGCTTGAGCCCAGTAGTTTGAGACCAGCCTGGCAATGT2990.1803638644918444No Hit
CCGCCTGGGAGCTCCTTTCACTTAACTGAAAGTCCAATGCAACCTCTGATAA2830.17071228645883602No Hit
GAGCAGGGGGCTACTTGACAGTTTATAAAATAGTTATAAGCCCAGATTTCTC2650.15985426117170157No Hit
TTAGCGGATTGCACTAAATTTGTTGAACCTGTGGTAGGCACATCTCTTAGGA2630.15864781391757551No Hit
TCATGTGGGACATGTTTCTAGACAGCTGTTAGATGCTTTAAATTCCAAATAG2610.1574413666634495No Hit
CCTATTGGGGAGAAACAAAGACTTCCAAGAGGTCACCCTTGAGAAGAACGGA2590.15623491940932344No Hit
GGCAATGGGAGTTGCAAGTGTTGAACAAGTTACTCTTTTGAAGCTGATGTGC2560.15442524852813433No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2550.15382202490107133No Hit
CAATACGGGTACATTTTTGTATCATAATAGATTTTAAAAATCTAATGTTCTT2540.1532188012740083No Hit
AAATTGGGAAGAAGCTGCTATGAGGTCTCGTAAACAGGGTCTTGACAATGTT2490.15020268313869317No Hit
TACGACGGGGCTATACTATTCGTTTGTTCTTATTAAGCCTTATTGTGTGTAA2460.14839301225750412No Hit
CAGTAAGGGCAAAACAATATTACTTTGTCTTTTCAATCTTCTACTTTTAACT2430.146583341376315No Hit
TTATCTGGGATTCCAAGAATTAAAATATAGAAATGGGTATATCAACCCAAAT2380.1435672232409999No Hit
GGAAATGGGATGTCCGAGGTTGGACAGGCAGTAGCTGGACAGATGCCATTGC2380.1435672232409999No Hit
ATACGAGGGGAGATCCTAGAGGGAGTCAGGCTGAAGAGGCGGGACTTTCCTG2340.1411543287327478No Hit
AGCGCAGGGATTTTAGCTTCACTTCTGACTGAACAGTCATTAGTCAACTTTT2310.13934465785155872No Hit
CAGAGGGGCCCTTATACCTCTCGGATCTTGAAACCTTATATCAGGCGTGATT2310.13934465785155872No Hit
TTGTTAGGGGTGGAAGTGGCTGGCCTGACATCCTTCTCCTTTGGGGAAGATG2300.1387414342244957No Hit
GTTTGGGATACCAAATATTGGGAGGACCTGGTGAAAGATGCTTTTAGGATCT2280.13753498697036964No Hit
ATCTTTGGGAGGGTATTTACAAGAAGACAAGTGGAAGAATTGGGGATCTTTG2260.13632853971624362No Hit
TTTTATGGGAAATGGAAACACTATGTAATGAAGGTTACTTCTCTATAAAATG2260.13632853971624362No Hit
GGACGAAGGGATTACATGTTCTAAATGCTTTACTTGTCGTATTTAATCCTAA2240.13512209246211757No Hit
GATCATGGGGAAGCTGCCAGCCTATAAGGCACAGTAGGATGGTGTGGACTAT2230.13451886883505454No Hit
TTTTATGGGAGATATCAAATTCATGAATATCAACATTGGTATGAAAAGTCAC2230.13451886883505454No Hit
GGTAATGGGGCAAAGAACCTAAATAGACATTCTCAAAATAAGACATAAAAAT2220.1339156452079915No Hit
AGTACGGGACGACATGTATAGGTTACCTAGATGTTATGAGAAATTTTAGTTT2210.1333124215809285No Hit
CCATCAGGGCCATGAAGTCCTAAATCTTCTTTAAAGTCAGAATAGGTATCTT2190.13210597432680243No Hit
GTCTTTGGGATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGA2170.13089952707267638No Hit
CAAGATGGGGATCTAAGATGGGGAGCTCACCAACACCTACTGTCTGGTAGCG2170.13089952707267638No Hit
AACAAGGGGGGAATTTTCAAACTTACAGTGTAATGAAACCTATATATCCATA2160.13029630344561335No Hit
GGCGCAGGGAATCCTTTTTAGAAAACTGTTTTGTCACATCTGTGAGACAATG2150.12969307981855033No Hit
GAGATAGGGGGAAGCAGTGAGAGCTGGGAAGCTTCTTTTGGCTCTAGGTGAG2150.12969307981855033No Hit
GTAGAGGGGGTCACCCTGAAGCCCTTAGATTCCAACCCCTCAATCAGAGACT2150.12969307981855033No Hit
GAAAATGGGGAAACAAACATTATTGTTGAGAGTTTGTTTATCCTATGGTGCT2110.12728018531029825No Hit
CAAGCTGGGACTGTGAGAGTAGGACCCAGATCCACCTTGCTCACTTCTGTGT2100.12667696168323522No Hit
CACTTTGGGTCTTTTGCCGGGACAGTGTTTTTTAAAAAGACACAAAAAAACT2090.1260737380561722No Hit
ATTTTTGGGCAAGGCGGGGCCTCCACACCACGAGTCCCATGCCAAGGTGATG2080.12547051442910914No Hit
TTTTGCGGGAATGTCATAATGGCCTCCAGGGCAGTGAGATTTGAAAGCCCTC2070.12486729080204613No Hit
TCATAAGGGAGAAAACAGGAAGAGAAATATGCATAAACCAGGGCAGCTCATT2070.12486729080204613No Hit
TGATAAGGGGGGATTTCATTTTGGTTCCATCAAATTAGGAAAAGAAATGGCT2050.12366084354792008No Hit
GGATTGGGACAAGAAGCTTAAGAAGAAGGATAAAGGGAAGCTGGAAGAGAAG2050.12366084354792008No Hit
TGGCTGGGGGGGAGGAGGAAGGTGGAGAATAGCAGATTGTGGGTGATATAAT2050.12366084354792008No Hit
CAATCGGGGCTTCTAGGAACTGCCTGGTTTTCAAGCAGGTCCTGGCTGAGCG2050.12366084354792008No Hit
CTGATGAGGTCCAGAAGCAATTTTATAACAGATTTCATTTTAACAAAATACT2050.12366084354792008No Hit
TGATATGGGGCGGAATGTCTGGATTAAGAAGCATTACCAATAGGAATGGATC2050.12366084354792008No Hit
TAATGGGGGATAAGATCTATTAAGCTTGACACATCTGTGTCATCACGCACTG2030.12245439629379404No Hit
TCAAAAGGGACTGACACAGGCCTGGTTCATGCATAGAATGTCAAGTGGGAAA2030.12245439629379404No Hit
GTAGACGGGAGGAATTTTGATTGCATTAATTTGTGGTTTAGTGAAGGGAGAA2030.12245439629379404No Hit
TTGTTGGGGATGGGGCAAAATGAGAGGGCTTCACTTTGTCCCTCACCTGCCC2030.12245439629379404No Hit
GTTCCTGGGCACACACACACACAAAACAACAGCAACAACAACAGAACAACAA2020.12185117266673101No Hit
ATTGCTGGGGTAAATGATGTCTGTAATGGGTTGAGTTACTGAGATGACAATC2020.12185117266673101No Hit
TGCACTGGGAGCTCTCAGAAGCGCTACCGGGCCGCCAGTGCCTTCTTCACCT2020.12185117266673101No Hit
CTTAGTGCGGGACCTCACAACAACCATTTGCCTCCTTTAAGAACACTGATGG2010.12124794903966798No Hit
CAAAATGGGCACATCATAGAAAGCATACAAGTCACAAATAAGGAGATAAATT1990.12004150178554195No Hit
TAGAACAGGGAGTATCATACGTTGATAGTTTACAAACAAGATACGTATATTT1990.12004150178554195No Hit
GGCACAGGGACATTTGAATATGATTTGATGTGCTGTTTGATAAAAGAACATC1990.12004150178554195No Hit
GTAGAGGGGATTAGCCATGTGATCAGGGGCAAATCATGTAATCATCTGTTTT1970.11883505453141589No Hit
TATACTGGGGTCTAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCG1960.11823183090435285No Hit
TAAGATGGGGATGAGGTTCTAGGACATTTACAAAATTCCATGTAACCTGTGT1960.11823183090435285No Hit
ACAGTGGGGTAGCAAGACTCTGTGGAGTTTGTTCAGTGGTACGGTGTCCAAG1940.1170253836502268No Hit
TACGTGGGAAGACTTGTGGGAAATACAAAATTGTAAAAGAGGTTTTTGTTTT1940.1170253836502268No Hit
GTATATGGGGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAAT1930.11642216002316379No Hit
CTTTAGGGGGGATTGGTTTTAATTTTTATGAGTTTGTCTCTGCAGTGATTCG1930.11642216002316379No Hit
TGCTCAGGGATCTTGATTTCCTTTTCACATCAATACTCTAAGATCACTATTT1930.11642216002316379No Hit
ATTATGGGGATGTTTAAAGCATTAATAAAATGTGATGAAGTACTTGCACCTG1930.11642216002316379No Hit
TACTCGGGATTTTCATTGATCAGAAATATTGGAATAGCATGAAATAATTTAG1930.11642216002316379No Hit
TTAAGGGGGAACACATGGTTAGAAGGGATAATTTATACTTCAATTTTGAGGA1920.11581893639610076No Hit
AGTTATGGGAACTGTAATAATAACCCATTTAACTTTATTGCTTTTCTTTTAT1920.11581893639610076No Hit
ACCACAGGGACGACAGTTTTCCGGCCTAGTTTGCGGCTGCTCCTGCCGTGTT1910.11521571276903773No Hit
GGGTACGGGGCTTTTAAGGTGTTTGTTTAGGGATACAATGACCACTAGATGT1910.11521571276903773No Hit
CACTGGGGACCAGGGGTTAGAAGTTGCCTCATTGGTTTAGTTCCTTGAGTGT1910.11521571276903773No Hit
TTTCTGGGATTACTGGTGATAAAGAGGTTCATTTTATAATCATGAAGGTTTG1900.11461248914197472No Hit
ATAGCTGGGGAGCCTTCAGATGACTGTGGTCCCATCCCTTATCTGATGGCAA1900.11461248914197472No Hit
AGCCTGGGCGACAGAGAGAGACTCCGACTCAAAAAAAATAAATAAATAAAAA1880.11340604188784867No Hit
ATTGTGGGGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGTGCTC1880.11340604188784867No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1880.11340604188784867No Hit
ACATGGGGGTACCTGTAAGAATGATTACACAGACATGATAAAAGCGCTTTTA1880.11340604188784867No Hit
CAAGTAGGGAGGAGAGGTAGGTAAATAAGAAAGATCCTGTGACTTAGGATGC1880.11340604188784867No Hit
TCTCGCGGGAACTACTTTAAGGGGAAGATAAGATTTCTGTCTACTAAGTGAT1880.11340604188784867No Hit
ACGGAAAGGCATGACTGGGAGGCCCACAAGGACTCTCATTGAGTTCTTACTT1870.11280281826078564No Hit
TACCCAGGGATGTCAAAGAGCCAGAAAGGCGGGGTCCTCAGAAACGACTGGC1870.11280281826078564No Hit
GGTATGGGGCCGGCTGCCGCATCATCGGGCGCATGTGTGTGGGGAACAGGCA1870.11280281826078564No Hit
CTGTGGCGGGCGATCTAAAAGTGGGCACTGGACAGACCACCCACTGCCGCCG1860.11219959463372262No Hit
GTATGGGGTCAGGGTTTAGCAGAGAAACAGAACCAATAGAATCAATCTATCT1850.11159637100665958No Hit
CTAGTTGGGCTATTTCTAAATTTACTTCTATCAGTGGATAATTCGTGTATAG1830.11038992375253354No Hit
GCAACGGGGGCTCCAGCGCTGACGCCGAGCCATGGCGGACGAGGAGCTTGAG1830.11038992375253354No Hit
ATCTAAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA1830.11038992375253354No Hit
TGTCAGGGATCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAAC1830.11038992375253354No Hit
TAAACAGGGACAAAAGCAGGAAGAAAGTCAGAGTTGTTTCAAGAGAAGAATT1820.10978670012547051No Hit
TCAACAGGGAGGGTTTGCGCTGCTATGAGAATCTAAGGCTGCCACTGATCTG1820.10978670012547051No Hit
CACATAGGGGTTTGGATTTTCTGTATACATTGAGGAAGCATTACCGGAATGA1820.10978670012547051No Hit
TAAGCAGGGGTAATAAAACTTGGTTGGCTTGATATTTTAAGGAATTGAAACC1810.10918347649840748No Hit
TACTGCGGGCAAACAGCTCGATAAGCCTTTTTGACTGTGTACATCTGTACCG1810.10918347649840748No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1810.10918347649840748No Hit
TAACGTGGGAAACGTTTAACGTATAATGTCTGTTTGGATACTGTTCCAAATT1810.10918347649840748No Hit
ATGATGGGGATCTGGTTCTTGGGATTTTTTTTCATAATTAAAATTATGATAT1810.10918347649840748No Hit
CTAGGGAATGAAATCACTTCATAAGGTGTCAAAAGGTATTCAAATGTTACGT1810.10918347649840748No Hit
GCTCGGGATGGACCCAAACTAGACTATACTTGGATAAGTTAAGCTCTTCTTC1810.10918347649840748No Hit
TCGAAGGGGAATTGGTTGACTTAATATATTTGCTTTTTTTCTCACCTGCACT1800.10858025287134446No Hit
CCGAACGGGATCCCTGGATTGAAGCGCAAAGCTGAATGAAAACGCCCAAAAT1800.10858025287134446No Hit
AGCCTGGGATGCTCAGTTTCTTATTAGTTTCATTGCTTAATTCCTGCTTAAG1800.10858025287134446No Hit
TAAGCACGGGATTTTTCTAATTCTAACAAGTTAGAATGTGAGGAAGGAATGA1800.10858025287134446No Hit
AAATTGGGATGTACTCAATTTTTTATGAATTATTTCACTCAGCATAATAGTT1800.10858025287134446No Hit
AATATCGGGCATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCT1790.10797702924428144No Hit
GCCTACGGGATTAAACTAATAATCCTAACAGCTCCATTGTCTTGCCTTTGCT1770.10677058199015539No Hit
TGAAATGGGATAGGCAGAAAACAAATTTAGTAGGATTATAACATTTTCTATA1770.10677058199015539No Hit
TAAACTGGGATCCTTTCTCATGGAAGCGCGTACCCGTTCGTGGAGGAAACGG1770.10677058199015539No Hit
ACACGAGGGCCTCATCTCTGAGATGAGTAAAATCTATGTGTGTTGCTATGGG1760.10616735836309237No Hit
TGTCAGTGGGATAGCTGTGGTTATGTTATTTTTTATTTAAAAACTTTATATT1750.10556413473602935No Hit
AAGACTGGGAGCAAAACATAATGAAGATATTTGTTAAAAACCTGTGACAACG1740.10496091110896633No Hit
TTTAACGGGAAGGAATAGTAGGGGGAAAGTTAATGTGAGCAAGATTTTTACT1740.10496091110896633No Hit
GCACTCGGGATTTTTGTATATAACTGGTTAATTTTAATAAGAGATACCATTA1730.10435768748190329No Hit
ATGAATGGGATGCCAATCACTGAGACTGCATCAGAGTTTGCAATTTTGTATG1720.10375446385484026No Hit
AACGAGGGATTCAGTAGGTTAGAAATCTGTGACTTAAGAGTGAATTTAGGCT1720.10375446385484026No Hit
AAGACCGGGATTCTACTTCACATCAACAGAACATATATTTTTTCTCATCTGT1720.10375446385484026No Hit
TCTTATGGGAGGTTTGTGAAATTGGTTCTTTGTATGGGGGATGGTCCTTTTT1710.10315124022777723No Hit
TCCCAAGGGCATTCCTTAGACAGCAGTGGTCTTTATCCCTTTCTGGAAAGAA1690.1019447929736512No Hit
ATCCTTGGGGAAGCCAAGTCCCAGGTGTCCTGGCTGCTCTCCTGGGCGGGGT1690.1019447929736512No Hit
AATACTGGGAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTGT1690.1019447929736512No Hit
TTTGGAGGGATCGTCGATTTGGTAGTTCAATTTTAACTACTCAATGAAAACA1680.10134156934658817No Hit
ATACGAGGGCTGGGGAAGGGATAGCATTGGGAAAAATACCTAATGTAGATGA1670.10073834571952514No Hit
TCTAGCGGGCAGCTCTGAGGGCAGCTCAATGAAAAAGTGAATGCACACGCCC1670.10073834571952514No Hit
AAATCAGGGTGTGTGACTATATTCCATAGAGAAGAAAGATGGGATAGCTCAA1670.10073834571952514No Hit
GACACGGGGATTTATCAAAATATTAATTGGACGTAATCTTCAATTTAGCAGT1670.10073834571952514No Hit
TGGCCTGGGAGAACCAACAAGGGGCTGAGTAGCTGAGAAAGGGGCCACCCAA1660.10013512209246211No Hit
ATGGGTGGGGATCATTTCCAATCCACTGTTTAAATGCAGAAATAAGTAGCAG1660.10013512209246211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)