FastQCFastQC Report
Sat 31 Dec 2022
SRR5849044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61223
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC20063.2765463959623014No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT19923.253679172859873No Hit
GCATCAGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA3710.6059814122143639No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3550.5798474429544452No Hit
CTTAGTGGGACGGCGTTGTTCTGGATTCCCGTCGTAACTTAAAGGGAAACTT3270.5341129967495876No Hit
TATCGCGGAATCCCTGATATCTTTGGAACCTGAAAATGTTACCTTATTTGGA3030.4949120428597096No Hit
TCTAGTGGGACCGGGCGCTGACCGTGCCCGAGCTCACCCAGCAGATGTTTGA2980.486745177465985No Hit
GCCAGTGGGTAGGAACAGAAGCTGTTCAAGACCCCACGAAGGTAGAAGCCCA2910.4753115659147706No Hit
GTGTACGGGAATGCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAAT2790.45571108896983165No Hit
CGCTGAGGGGGGGCGGAGAGGCCTGGCGCACAGGGCGAGGGCGGCTGCGGCG2780.45407771589108664No Hit
AGCTCTGGGGTCCTGTCACACCCTCTGCACTCGCTGAGCTTCTTGACCTGCT2780.45407771589108664No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTC2740.447544223576107No Hit
TAGGTTGGGATGCTCCAAGGATTGAGGTCATGACCATGGGAATAAAGTTATT2730.44591085049736207No Hit
TTTAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAG2720.4442774774186172No Hit
TATCGCGGGGATCTATGACATCTGTCGTAGAAACCTCGATATTGAGCGTCCA2680.43774398510363755No Hit
GATGCAGGGCTGGATTAAGAGAAGTTCTTTCTGTTAAACTCACCTAGGGTAG2670.43611061202489265No Hit
CAAAGGGCTCCTCATAGCCTTCGGCAGTCTCCTCTGAAAACACACTGCCTAC2630.4295771197099129No Hit
GAATATGGGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAG2610.42631037355242307No Hit
AGTTGCAGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC2610.42631037355242307No Hit
AGTAAAGGGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCA2560.41814350815869855No Hit
TCTCCTGGGGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGG2560.41814350815869855No Hit
AACAGCGGGGTGAGTTACAATGACACCGCTCCCCGGATCCTGTTCTGGGCCC2440.3985430312137595No Hit
GTCTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2420.3952762850562697No Hit
AAGCAGGGTAAGTATATTTAATGAAATTGGTGGTTTTAGGAAGTCAACTTTA2420.3952762850562697No Hit
GTCCACGGGTCTACCATGATCATACAAAAATCATCATCTGTAGCCAAAATGA2420.3952762850562697No Hit
TTAAGTGGGGGCCGGGCCTGAGGGCGTGTATCCGCGGCCGTAGCAGCCGGGC2350.3838426735050553No Hit
AGCACAGGGATAGGAAATTATAGGAGATTTATTCAGTTTCTTCATGGCTGTG2310.3773091811900756No Hit
ATTTCTGGGAAGAACCTGTTAATAGGTTGGAAAGCTGTGTACTCTATTCATA2290.3740424350325858No Hit
CGCCGAGGGGGAGGCGCCGGCGCCCTGGATCCGCCGGCCGTGGTCGCCGAGT2290.3740424350325858No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2250.36750894271760615No Hit
CAAACAGGGCCTCTGAATATGGGAAGGTTGTATTCCCAGATTCTTTCCTGAA2230.3642421965601163No Hit
ATTATAGGGACCCAAGGGCTACTTCCTGACCAAGATCTTCCACCCGAACGTG2220.3626088234813714No Hit
CTAGCAGGGCTTTTTCTTCAGCGAGGCGGCCGAGCTGGTTGGTGGCGGCGGT2200.3593420773238815No Hit
GGTCTTAGGGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCC2200.3593420773238815No Hit
TCGCATGGGAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGACAC2190.3577087042451366No Hit
CAGTCTGGGGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGT2140.34954183885141205No Hit
CATGTTGGGAAACATGAAAAATTTAAAAATCAGAAAAGAAAGAAAAACATTA2130.3479084657726671No Hit
GGCTTTGGGGGCGGGACTGCGCGGCGGCGACTACAGACGTGTCGGGGGTCCG2110.3446417196151773No Hit
AGCCTCGGGAGAAGCAGCATAGGAACAATAAGAACAATAGGTAAAGCTATAA2100.3430083465364324No Hit
CTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC2090.34137497345768747No Hit
CATAGTGGGTAGTAACGGGATTCCAGGAAATTTATTTTTGGGTTGTCCTACA2040.3332081080639629No Hit
CTCAAAGGGGCGGGCTGCCGGGAACGGGGCGGAGCGCGGCTGCGCCGGCGCG2000.32667461574898327No Hit
ATCTTCGGGAAATGAATGAAATTGTGATTTCCTGAGAATCGAACCTTGGTTC1970.3217744965127485No Hit
GTGACTGGGGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAAT1970.3217744965127485No Hit
TTTGGTGGAAGGCTGTATGATGACCACACGTTCCTAAGCTGGAGTGCTTATC1970.3217744965127485No Hit
ACAGTTGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGACGCCGAGCCATGGC1950.31850775035525863No Hit
CGATTAGGGCCCTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCA1940.31687437727651374No Hit
CCTTCAGGGGCTCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA1880.3070741388040442No Hit
ATGCTTGGATATATTAAATTAAAAAAACTCAAACTCACAGAAGCAGGAAGTA1880.3070741388040442No Hit
TGTTATGGGGGACGATACCATCGCTTCAGCAGCATCCTCTCAGACAAGAGCC1850.3021740195678095No Hit
CGCTTTGGGCCGATCTGTGTGGGGAAGGTGGTTAGGTTTTAAAGACCGTGCC1820.29727390033157475No Hit
TCACACGGGAGTCACCTGAAGAAGGAAAGCGACAAGCCACTGTAGAACCTGC1750.28584028878036033No Hit
GTTTGAGGGAGTGGCATTGATAGTGATTATTCACAACATACATTATACTCAT1740.2842069157016154No Hit
TCGCAGGGGCCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAG1740.2842069157016154No Hit
ACCACAGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACG1710.27930679646538065No Hit
GCATCGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACA1690.2760400503078908No Hit
ATGCTTGGGAGGTGAGAAGAAAGGAGAGAAGGTTTTTAGGTTAGAAACCTTT1670.27277330415040096No Hit
CAAATGGGGGGTGATATAGTTTTTAAAAATAACTATTTTGTTATAGATCATA1630.26623981183542134No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1610.2629730656779315No Hit
CATCGTGGGATGGGAAATCTAGGCGAAAGTAATGGGGAAGATTAGAAAGAAT1580.25807294644169676No Hit
TCGCTGGGGATCTGATCTACCCCATCTACCCCTGGATTTCTGCAAGATGTTC1570.2564395733629518No Hit
TTACCTGGGTGGTTCGGCAGACTCCTACACTAGCCGTCCATCCGATTCCGAT1570.2564395733629518No Hit
ATGTTGGGGATCGATGAAACCGATAACATTGGCCTCATTGGATTTCTTTACC1560.2548062002842069No Hit
CGTGCTGGGCTCAGCTTCACAGTATCCAAAAATGTAAATATCATGCCATCAA1520.24827270796922724No Hit
TGCCACGGGACTCTTTTAGTATAAATAGTACCGTTAACTTCCAATTAACTAG1520.24827270796922724No Hit
CATTTCGGGTATAGCAGCAAAATAAGTGAGGTAATACACATGCCATATCAAT1510.24663933489048234No Hit
ATGCTTGGGTATGAATCTCAGGAACACCACCTTCTGTCAGATGGTGAGAACA1480.2417392156542476No Hit
AACGCGGGGGGACAGGGTCATCCTGAGGGTGCGACTCCGCCGCGATGGTGAC1470.2401058425755027No Hit
TGCCTGGGAGACGATCAGTGGGAAGGCCTTTCCTAGGAGGTAACCAGAACAG1470.2401058425755027No Hit
GACACAGGGCCGGGAAAGCCCTCTGCCCACACCCACAGGCTTCACATTCCCA1460.23847246949675774No Hit
TTCCAGGGGACCAGCCAGTTTCTCTATTTTACACATAAGAAAAATGAAACCA1440.23520572333926792No Hit
CTGCATGGGGTATGAGCTGGGTGTTTTTTTCCATTACTTTTAATGATCTTCG1440.23520572333926792No Hit
CGGCCTGGGTACACAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1440.23520572333926792No Hit
AGTGCTGGGATTGCAGGCGTGAGCCACTGCACCCAGACTTGTGAGGTTTTAA1420.23193897718177808No Hit
TATGCAGGGGAGAAATCTCTGTATTCTGGTTGGTTCATATGTACAGAATATA1420.23193897718177808No Hit
TTAATAGGGACCAACCCAGTCTCAACACACAAAGTGCCACCACCTTCCCTGA1420.23193897718177808No Hit
GGGTTAGGGGTGTACCCCTCTATATATATGTTACATAGAATGTATATATGTT1420.23193897718177808No Hit
TGTCAAGGGACCAAAATAAAATCAAACAGGAAATCGATAAAAGAAAGATAAC1410.2303056041030332No Hit
TTAGCAGGGGTAGCTGTAATTTTCTGACAAAATTTAAGACAAAATTGTCAAC1410.2303056041030332No Hit
AGCCTAGGGGAGGGGCGGACCAAGGAGCTGGGCTACACGGTGAAGAAGCATC1410.2303056041030332No Hit
CTAGTCGGGACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGA1380.2254054848667984No Hit
AAGTTGGGAAGCTTATGTGGTACAGTAAGTGTTCTGTTCTGAGAAATACCTT1370.2237721117880535No Hit
GCCGAGTGCTCTGATGCAATAGTGGAAATCGCTTATATGTCCTTGGGTTGCT1330.21723861947307385No Hit
TGTAAGGGGTAATGACGGGGAAGCAGAAAGGTGCCGACTTCCTGGTGACGGA1320.21560524639432896No Hit
AGTCTAGGGATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAA1310.21397187331558398No Hit
AATACGGGGAGACATATGAGACCTGAAGGTTGTACCTTGAGTTTGGAGGAGG1310.21397187331558398No Hit
GGTCCAGGGGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGG1300.2123385002368391No Hit
ATAGTGGGGGGAGCTCAGAGCTGTTCAGCCCAGGAAAACAAAGTGACTAAGA1280.20907175407934928No Hit
GACTTAGGGTCTATTACAGGGATTGAAAAAGTAGTATATACTGTGAAGCAAA1270.20743838100060435No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1270.20743838100060435No Hit
GCCGTTGGGTGAGTGCACGATGAATGATTTTAAGGGTTTCCAGGTCACTGCC1270.20743838100060435No Hit
ATACTGGGGCTTCCGGCCGGGCCGAGAGGTGGTTACATTCGTTGAAGGACAC1250.20417163484311454No Hit
TGTAAAGGGATAGTTTTTAAATTATTGTTTAAAAAACAAAACAGCTGTTATA1240.20253826176436956No Hit
CGAATTGGGGGGAGTTTCCAAGTATGGCATAAAAGTATCAACATCAGATCAA1230.20090488868562467No Hit
TGTGATGGGAAAGCTATAATTTTTAGAAGATAACATTGGAAAAAATCCTCAG1230.20090488868562467No Hit
CGCCATGGGATGATCACGCCACTGCACTACAGCCTGGGCAACAAAGTGAGAC1220.19927151560687975No Hit
CACACCGGGACAGTAATTCTCTGTTGAAATGTAACATTTCTGTTTTACAGAT1200.19600476944938994No Hit
GTTATGGGGAAGGAAATGTTTGGTCTCCTATAAAGAAACCAGGCTCCAACCT1190.19437139637064502No Hit
TCATATGGGGGAATCGGTTAAGTCATGCATCTTAAAGATTTTGGAAAAGACA1170.19110465021315517No Hit
GAAACGGGGAACAGAAATATATTTTGATCTATTTTCCTATGGAGTTGTTTCT1160.18947127713441028No Hit
ATCTATGGGGAAAAAAAATTTCTATTCATAGCTTATCCTTCAGAGCAATGAC1150.18783790405566536No Hit
AAAGACGGGGATACATTTACAAAAATAAAAAGCGGGAATTTTCCCTTCGCTT1130.18457115789817552No Hit
TCTTCAGGGATTCTAATGAAGTCCAGTTTTATCACTTTTTCCTTTTGTAGAT1120.1829377848194306No Hit
TAAGATGGGTAAACAGTCAATGAGAAAAATAGTGCATATAAACCAGTAAAAG1120.1829377848194306No Hit
AAAATTGGGGAAGCTCACCCCTTGGAGAGCCTACAGAACCTCAGGCTGATAG1120.1829377848194306No Hit
TGATTTGGGAGATCACCTGGAGTTGATTATATGAGTCTATGCTTTTGAGCTC1120.1829377848194306No Hit
TTTGTAGGGGGGGGGCCGGCCGAGAGGAAAGCTGGAGGCGCGGGTGGGGAAC1120.1829377848194306No Hit
GAATCAGGGCACCCTTCCCAACTTGGTCTCCTCCAACATGCTGTCTTCATGT1110.1813044117406857No Hit
GGCTATGGGACATTGAGCTGTTACATTAGTTCAGGCTAAATGTTGGGAGCTC1110.1813044117406857No Hit
GAGCAAGGGGGCGGGGGCCGGCCGAGAGGAAAGCTGGAGGCGCGGGTGGGGA1100.17967103866194076No Hit
TTTAGTGGGAGGTTGGGCCCCTGGGGGATCTGAGGGCCTGTGGCCCCCAGGG1090.17803766558319586No Hit
TCTATGGGGGGAAGTGGCTCCTGTAAGGCAGCAAGGTAGCGTGGCCGGCGCC1090.17803766558319586No Hit
TTATTGGGATAAACATAAGGCTTAAAGTCTTGGATTTGATGCATATGACTTA1080.17640429250445094No Hit
TTTGCTGGGAGAATAAGAGCTTTTTTGTTTCTTGGGTTTTTAAAATGTGAAT1050.1715041732682162No Hit
TTAACGGGGGCCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCA1050.1715041732682162No Hit
CGCGAGAGGGAGAGAAACCCCGTCTGTACTAAAAATACAAAAATTAGGTCTG1040.1698708001894713No Hit
GAAATGGGGAGACACCTAGAGCCAAAGAAGTTATGTCCATGAAAGGTCAGGC1040.1698708001894713No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT1010.16497068095323653No Hit
GTTGCAGGGAGGGCCCAATTTGCCCTGCCTGGAGTTCCCCCTGGCTCTAGGA1010.16497068095323653No Hit
TCACTCGGGACAGTGACCGCCACCGTTGCCGGGGGATGGGTCCCTGAGACTT980.1600705617170018No Hit
TTACAGGGAGATGAGAACTATTATAGAGGTTATCATTGTGAAGTATTTTCAA980.1600705617170018No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC960.15680381555951195No Hit
GTGTGAGAGGAAAAGTGGGAAGTACGTCCTGGGGTACAAGCAGACTCTGAAG950.15517044248076703No Hit
GGTAATGGGGACTGATAGCTGTTTTAAGAGCCACTGGCCTGTAATTGTTTGA950.15517044248076703No Hit
GCCACAGGGAATGTTTTAGCAAGCCATTCCTAAACAGCCCAACTGGCATTTA950.15517044248076703No Hit
ATTCCAGGGAGATGCCTATGGGTGCAGTGCTTGGAGAACCAAATAAAACTAC940.1535370694020221No Hit
TACCGCGGGTCTTAAGCCAAAGGGACATATTTTGGGGTTGCATATTCTGGTT930.15190369632327722No Hit
ACACATGGGATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCT920.15027032324453227No Hit
TTCACTGGGGTAGCCATGGAAGATACTTTCAAAAGGACATCTTCAGATCATA900.14700357708704245No Hit
GCATACGGGCAGGGCCTGTTACAGTCTACCCCTACATGCATGCCCTGGACAC890.14537020400829753No Hit
CATGGAGGGCAGTGGAGGCAGGCGTGGCTGCATTCCCCTCACGCTACCCTCC890.14537020400829753No Hit
ATGTTAGGGGAATGGCTCAAACGCTTTTGTATACCTATTGTTCTATATAAAG880.1437368309295526No Hit
AAAAAAGGGAATTTTGAGCAGTTACACATTTATCTAAAGGAAATAAATCAAC870.1421034578508077No Hit
CTTGAGACAGGGATGTCATATATGTGTGCTTCATGTCATGCGAAGTATTATC860.1404700847720628No Hit
TATGCAGGGAGTGGCGTTTACATCTACTACACCAAGCTTCAGGGAAAGTCCC850.13883671169331788No Hit
GTTCCTGGGACTGATCGCATTGAAAACATTGATCACCTGGGTTTCTTTATTT850.13883671169331788No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT840.13720333861457296No Hit
TGGAACGGGATGATTTCAGAGCACATAAGTAAAGGTGCTTTTTAATGTGCAG830.13556996553582804No Hit
ATCTTGGGGAGTGAGCTGAGGTCGCGCCACTGAACTCCAGCCTGGAGACAGA830.13556996553582804No Hit
ACAAAGGGGACTGAATGAAGGAAACATGAATCAAATAAAGTTTTGAATTTTA820.13393659245708311No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCACCTCGTATGC810.13230321937833822No Hit
TCCTCTGGGATGTTCCTGCTGTGTTGCTCAATCTACTTTTCCTCTGGGGATC810.13230321937833822No Hit
GTCATTGGGCTGCCTAAGTTGTCTGCCTTTTCTACCACCAAAAAGACTTTTA800.13066984629959327No Hit
TCTGCAGTTAATATCAAATGTGAGTTTGGGACTGCCTGGCAACATTTATATT800.13066984629959327No Hit
ATGTTGGGGTCTTTTTGAGGAAGACGCGGTCGTAAGGGCTGAGGATTTTTGG790.12903647322084838No Hit
CTTTAGGGGCAACACCCTCTTCCTCACCATGGATGAGAACAAGATGGGAGTG780.12740310014210346No Hit
TATACTGGGATTGCCATCTCTGTTAGTGCAGCCATTAGAAAGACATTGTGCC770.12576972706335854No Hit
TTGAGCAGGGTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACC750.12250298090586871No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC750.12250298090586871No Hit
GTCTACGGGGAAATGACTGGTATGCAATATTTTTTAAAGCCTTCATTTTTGG710.11596948859088904No Hit
TGGCTGGGATAGATAGAGAATTACCATTATAACATTGCTCCATTGCCCACTC700.11433611551214413No Hit
ATCCACGGGTGCTCCTGGGTTTGAAGATGCAGGCCGATCGCCAGCTCCGTGG700.11433611551214413No Hit
TGAGTCGGGAAGATACCATGAATAATTTATGAACTCTGATGCTAAGAAATCC690.1127027424333992No Hit
TGCAGCGGGACTGCTGGAAAAGCTAGATGTGAGGGGAAAACAATGGGCATTA690.1127027424333992No Hit
GTAAAGGGGAGAATGTGAAGATCTCTGATTCTCACAGTAGTCTATTTAGTGT680.1110693693546543No Hit
CAAGCCGGGGTTACCATTTTTCTGGATTTAGTAAGAAATTTGCAGTTTTGGT670.10943599627590939No Hit
CGGGTCGGGGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAG670.10943599627590939No Hit
ATTGCTGGGAATTTCAAAGTCATTTATTGTGGAAGAATGACCAAGAAGTATG660.10780262319716448No Hit
CTTTGGTGGGGGCACAAGGGAAGAAGGTGAAAACCTGAGTAGGTATGTTTTG650.10616925011841954No Hit
CATAATGGGCTGCGCTAATTACCTGAGCGACCAGGACTACATTTCCCAAGAG640.10453587703967464No Hit
TACACAGGGCACCTGATTTTCATGACAAATACGGTAATGCTGTATTAGCTAG630.10290250396092972No Hit
ACCATAGGGTAGAAAAGGAGAAGGAGAGAATCAAGGTGTAAGTTGTCAATGG630.10290250396092972No Hit
TTAATAGGGAGATCTTTTACTCACATTCTGTTGTTCAGTCCTCTGCACTGGG620.10126913088218478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)