FastQCFastQC Report
Sat 31 Dec 2022
SRR5849043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60700
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC20583.390444810543657No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT18963.1235584843492585No Hit
TATCGCGGAATCCCTGATATCTTTGGAACCTGAAAATGTTACCTTATTTGGA3720.6128500823723229No Hit
CTTAGTGGGACGGCGTTGTTCTGGATTCCCGTCGTAACTTAAAGGGAAACTT3510.5782537067545305No Hit
GCATCAGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA3510.5782537067545305No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3410.5617792421746293No Hit
TCTAGTGGGACCGGGCGCTGACCGTGCCCGAGCTCACCCAGCAGATGTTTGA3270.5387149917627677No Hit
CGCTGAGGGGGGGCGGAGAGGCCTGGCGCACAGGGCGAGGGCGGCTGCGGCG3220.5304777594728172No Hit
TTTAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAG3130.515650741350906No Hit
GCCAGTGGGTAGGAACAGAAGCTGTTCAAGACCCCACGAAGGTAGAAGCCCA3010.49588138385502467No Hit
GTGTACGGGAATGCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAAT2950.485996705107084No Hit
CAAAGGGCTCCTCATAGCCTTCGGCAGTCTCCTCTGAAAACACACTGCCTAC2930.4827018121911038No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTC2850.4695222405271829No Hit
AAGCAGGGTAAGTATATTTAATGAAATTGGTGGTTTTAGGAAGTCAACTTTA2840.46787479406919275No Hit
GATGCAGGGCTGGATTAAGAGAAGTTCTTTCTGTTAAACTCACCTAGGGTAG2830.46622734761120266No Hit
AGTTGCAGGGGCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC2830.46622734761120266No Hit
TAGGTTGGGATGCTCCAAGGATTGAGGTCATGACCATGGGAATAAAGTTATT2800.46128500823723234No Hit
AGTAAAGGGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCA2730.4497528830313015No Hit
AGCTCTGGGGTCCTGTCACACCCTCTGCACTCGCTGAGCTTCTTGACCTGCT2670.4398682042833608No Hit
GTCTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2590.4266886326194399No Hit
CGCCGAGGGGGAGGCGCCGGCGCCCTGGATCCGCCGGCCGTGGTCGCCGAGT2510.4135090609555189No Hit
TCTCCTGGGGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGG2460.4052718286655684No Hit
AGCCTCGGGAGAAGCAGCATAGGAACAATAAGAACAATAGGTAAAGCTATAA2450.40362438220757824No Hit
TTAAGTGGGGGCCGGGCCTGAGGGCGTGTATCCGCGGCCGTAGCAGCCGGGC2410.39703459637561783No Hit
AGCACAGGGATAGGAAATTATAGGAGATTTATTCAGTTTCTTCATGGCTGTG2380.39209225700164746No Hit
AACAGCGGGGTGAGTTACAATGACACCGCTCCCCGGATCCTGTTCTGGGCCC2370.3904448105436573No Hit
GAATATGGGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAG2350.3871499176276771No Hit
ATTTCTGGGAAGAACCTGTTAATAGGTTGGAAAGCTGTGTACTCTATTCATA2350.3871499176276771No Hit
ATTATAGGGACCCAAGGGCTACTTCCTGACCAAGATCTTCCACCCGAACGTG2340.38550247116968694No Hit
TATCGCGGGGATCTATGACATCTGTCGTAGAAACCTCGATATTGAGCGTCCA2310.3805601317957166No Hit
CATGTTGGGAAACATGAAAAATTTAAAAATCAGAAAAGAAAGAAAAACATTA2290.3772652388797364No Hit
CTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC2280.3756177924217463No Hit
CAGTCTGGGGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGT2250.37067545304777594No Hit
GGTCTTAGGGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCC2220.3657331136738056No Hit
CGATTAGGGCCCTTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCA2180.3591433278418451No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2170.357495881383855No Hit
CAAACAGGGCCTCTGAATATGGGAAGGTTGTATTCCCAGATTCTTTCCTGAA2160.35584843492586493No Hit
GGCTTTGGGGGCGGGACTGCGCGGCGGCGACTACAGACGTGTCGGGGGTCCG2120.3492586490939044No Hit
GTGACTGGGGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAAT2090.3443163097199341No Hit
ATCTTCGGGAAATGAATGAAATTGTGATTTCCTGAGAATCGAACCTTGGTTC2070.34102141680395387No Hit
CTAGCAGGGCTTTTTCTTCAGCGAGGCGGCCGAGCTGGTTGGTGGCGGCGGT1930.31795716639209226No Hit
TCGCATGGGAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGACAC1910.31466227347611203No Hit
CTCAAAGGGGCGGGCTGCCGGGAACGGGGCGGAGCGCGGCTGCGCCGGCGCG1880.3097199341021417No Hit
ATGCTTGGATATATTAAATTAAAAAAACTCAAACTCACAGAAGCAGGAAGTA1870.30807248764415157No Hit
ACAGTTGGGGAGAGTGACCGCGGCTGCTCCAGCGCTGACGCCGAGCCATGGC1860.30642504118616143No Hit
TCACACGGGAGTCACCTGAAGAAGGAAAGCGACAAGCCACTGTAGAACCTGC1830.3014827018121911No Hit
CCTTCAGGGGCTCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA1820.29983525535420097No Hit
GTCCACGGGTCTACCATGATCATACAAAAATCATCATCTGTAGCCAAAATGA1820.29983525535420097No Hit
CATAGTGGGTAGTAACGGGATTCCAGGAAATTTATTTTTGGGTTGTCCTACA1810.2981878088962109No Hit
ATGTTGGGGATCGATGAAACCGATAACATTGGCCTCATTGGATTTCTTTACC1800.29654036243822074No Hit
TCGCAGGGGCCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAG1800.29654036243822074No Hit
TTTGGTGGAAGGCTGTATGATGACCACACGTTCCTAAGCTGGAGTGCTTATC1780.29324546952224056No Hit
CATTTCGGGTATAGCAGCAAAATAAGTGAGGTAATACACATGCCATATCAAT1760.2899505766062603No Hit
AGTGCTGGGATTGCAGGCGTGAGCCACTGCACCCAGACTTGTGAGGTTTTAA1750.2883031301482702No Hit
TTACCTGGGTGGTTCGGCAGACTCCTACACTAGCCGTCCATCCGATTCCGAT1750.2883031301482702No Hit
GTTTGAGGGAGTGGCATTGATAGTGATTATTCACAACATACATTATACTCAT1730.28500823723228996No Hit
TGTCAAGGGACCAAAATAAAATCAAACAGGAAATCGATAAAAGAAAGATAAC1680.27677100494233936No Hit
GGGTTAGGGGTGTACCCCTCTATATATATGTTACATAGAATGTATATATGTT1680.27677100494233936No Hit
ACCACAGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACG1670.2751235584843493No Hit
AGTCTAGGGATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAA1670.2751235584843493No Hit
GCATCGGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACA1670.2751235584843493No Hit
AGCCTAGGGGAGGGGCGGACCAAGGAGCTGGGCTACACGGTGAAGAAGCATC1640.2701812191103789No Hit
CATCGTGGGATGGGAAATCTAGGCGAAAGTAATGGGGAAGATTAGAAAGAAT1640.2701812191103789No Hit
CTGCATGGGGTATGAGCTGGGTGTTTTTTTCCATTACTTTTAATGATCTTCG1620.26688632619439867No Hit
CGGCCTGGGTACACAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1610.2652388797364086No Hit
TGTTATGGGGGACGATACCATCGCTTCAGCAGCATCCTCTCAGACAAGAGCC1590.26194398682042835No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC1580.2602965403624382No Hit
TCGCTGGGGATCTGATCTACCCCATCTACCCCTGGATTTCTGCAAGATGTTC1560.25700164744645804No Hit
ATGCTTGGGTATGAATCTCAGGAACACCACCTTCTGTCAGATGGTGAGAACA1540.25370675453047775No Hit
CGTGCTGGGCTCAGCTTCACAGTATCCAAAAATGTAAATATCATGCCATCAA1530.2520593080724876No Hit
ATGCTTGGGAGGTGAGAAGAAAGGAGAGAAGGTTTTTAGGTTAGAAACCTTT1500.2471169686985173No Hit
GACACAGGGCCGGGAAAGCCCTCTGCCCACACCCACAGGCTTCACATTCCCA1490.2454695222405272No Hit
TGTAAAGGGATAGTTTTTAAATTATTGTTTAAAAAACAAAACAGCTGTTATA1490.2454695222405272No Hit
GGTCCAGGGGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGG1450.23887973640856672No Hit
TTCCAGGGGACCAGCCAGTTTCTCTATTTTACACATAAGAAAAATGAAACCA1440.2372322899505766No Hit
GACTTAGGGTCTATTACAGGGATTGAAAAAGTAGTATATACTGTGAAGCAAA1430.23558484349258652No Hit
CTAGTCGGGACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCACGA1430.23558484349258652No Hit
AAAGACGGGGATACATTTACAAAAATAAAAAGCGGGAATTTTCCCTTCGCTT1410.23228995057660626No Hit
TGCCACGGGACTCTTTTAGTATAAATAGTACCGTTAACTTCCAATTAACTAG1400.23064250411861617No Hit
AAGTTGGGAAGCTTATGTGGTACAGTAAGTGTTCTGTTCTGAGAAATACCTT1390.22899505766062603No Hit
CGCTTTGGGCCGATCTGTGTGGGGAAGGTGGTTAGGTTTTAAAGACCGTGCC1390.22899505766062603No Hit
TGCCTGGGAGACGATCAGTGGGAAGGCCTTTCCTAGGAGGTAACCAGAACAG1370.22570016474464577No Hit
AATACGGGGAGACATATGAGACCTGAAGGTTGTACCTTGAGTTTGGAGGAGG1360.22405271828665568No Hit
TTAATAGGGACCAACCCAGTCTCAACACACAAAGTGCCACCACCTTCCCTGA1350.22240527182866557No Hit
GTTATGGGGAAGGAAATGTTTGGTCTCCTATAAAGAAACCAGGCTCCAACCT1330.21911037891268534No Hit
TTATTGGGATAAACATAAGGCTTAAAGTCTTGGATTTGATGCATATGACTTA1320.21746293245469522No Hit
CAAATGGGGGGTGATATAGTTTTTAAAAATAACTATTTTGTTATAGATCATA1320.21746293245469522No Hit
GAAATGGGGAGACACCTAGAGCCAAAGAAGTTATGTCCATGAAAGGTCAGGC1310.21581548599670508No Hit
CGCCATGGGATGATCACGCCACTGCACTACAGCCTGGGCAACAAAGTGAGAC1300.214168039538715No Hit
TATGCAGGGGAGAAATCTCTGTATTCTGGTTGGTTCATATGTACAGAATATA1270.20922570016474468No Hit
TGATTTGGGAGATCACCTGGAGTTGATTATATGAGTCTATGCTTTTGAGCTC1260.20757825370675453No Hit
TTAGCAGGGGTAGCTGTAATTTTCTGACAAAATTTAAGACAAAATTGTCAAC1260.20757825370675453No Hit
TCTTCAGGGATTCTAATGAAGTCCAGTTTTATCACTTTTTCCTTTTGTAGAT1240.20428336079077428No Hit
TGTAAGGGGTAATGACGGGGAAGCAGAAAGGTGCCGACTTCCTGGTGACGGA1240.20428336079077428No Hit
GCCGAGTGCTCTGATGCAATAGTGGAAATCGCTTATATGTCCTTGGGTTGCT1230.2026359143327842No Hit
GGCTATGGGACATTGAGCTGTTACATTAGTTCAGGCTAAATGTTGGGAGCTC1210.19934102141680393No Hit
TTTGTAGGGGGGGGGCCGGCCGAGAGGAAAGCTGGAGGCGCGGGTGGGGAAC1210.19934102141680393No Hit
TAAGATGGGTAAACAGTCAATGAGAAAAATAGTGCATATAAACCAGTAAAAG1170.19275123558484347No Hit
AACGCGGGGGGACAGGGTCATCCTGAGGGTGCGACTCCGCCGCGATGGTGAC1140.18780889621087316No Hit
GAGCAAGGGGGCGGGGGCCGGCCGAGAGGAAAGCTGGAGGCGCGGGTGGGGA1140.18780889621087316No Hit
GAATCAGGGCACCCTTCCCAACTTGGTCTCCTCCAACATGCTGTCTTCATGT1130.18616144975288304No Hit
GCCGTTGGGTGAGTGCACGATGAATGATTTTAAGGGTTTCCAGGTCACTGCC1110.1828665568369028No Hit
TTTGCTGGGAGAATAAGAGCTTTTTTGTTTCTTGGGTTTTTAAAATGTGAAT1100.1812191103789127No Hit
CGAATTGGGGGGAGTTTCCAAGTATGGCATAAAAGTATCAACATCAGATCAA1080.17792421746293247No Hit
CACACCGGGACAGTAATTCTCTGTTGAAATGTAACATTTCTGTTTTACAGAT1080.17792421746293247No Hit
ATAGTGGGGGGAGCTCAGAGCTGTTCAGCCCAGGAAAACAAAGTGACTAAGA1070.17627677100494235No Hit
TTTAGTGGGAGGTTGGGCCCCTGGGGGATCTGAGGGCCTGTGGCCCCCAGGG1060.1746293245469522No Hit
CGCGAGAGGGAGAGAAACCCCGTCTGTACTAAAAATACAAAAATTAGGTCTG1040.17133443163097198No Hit
AAAATTGGGGAAGCTCACCCCTTGGAGAGCCTACAGAACCTCAGGCTGATAG1020.16803953871499178No Hit
ACACATGGGATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCT1010.16639209225700163No Hit
GTTGCAGGGAGGGCCCAATTTGCCCTGCCTGGAGTTCCCCCTGGCTCTAGGA1000.16474464579901155No Hit
TTACAGGGAGATGAGAACTATTATAGAGGTTATCATTGTGAAGTATTTTCAA1000.16474464579901155No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT990.16309719934102143No Hit
ATCTATGGGGAAAAAAAATTTCTATTCATAGCTTATCCTTCAGAGCAATGAC980.1614497528830313No Hit
ATACTGGGGCTTCCGGCCGGGCCGAGAGGTGGTTACATTCGTTGAAGGACAC970.1598023064250412No Hit
TCCTCTGGGATGTTCCTGCTGTGTTGCTCAATCTACTTTTCCTCTGGGGATC970.1598023064250412No Hit
TCTGCAGTTAATATCAAATGTGAGTTTGGGACTGCCTGGCAACATTTATATT970.1598023064250412No Hit
ATCTTGGGGAGTGAGCTGAGGTCGCGCCACTGAACTCCAGCCTGGAGACAGA970.1598023064250412No Hit
TGTGATGGGAAAGCTATAATTTTTAGAAGATAACATTGGAAAAAATCCTCAG960.15815485996705106No Hit
GTGTGAGAGGAAAAGTGGGAAGTACGTCCTGGGGTACAAGCAGACTCTGAAG950.15650741350906094No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT950.15650741350906094No Hit
TCTATGGGGGGAAGTGGCTCCTGTAAGGCAGCAAGGTAGCGTGGCCGGCGCC930.15321252059308071No Hit
CTTTAGGGGCAACACCCTCTTCCTCACCATGGATGAGAACAAGATGGGAGTG910.14991762767710048No Hit
GAAACGGGGAACAGAAATATATTTTGATCTATTTTCCTATGGAGTTGTTTCT900.14827018121911037No Hit
TATGCAGGGAGTGGCGTTTACATCTACTACACCAAGCTTCAGGGAAAGTCCC890.14662273476112028No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGT870.14332784184514002No Hit
TCACTCGGGACAGTGACCGCCACCGTTGCCGGGGGATGGGTCCCTGAGACTT870.14332784184514002No Hit
CATGGAGGGCAGTGGAGGCAGGCGTGGCTGCATTCCCCTCACGCTACCCTCC860.14168039538714994No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC850.1400329489291598No Hit
TCATATGGGGGAATCGGTTAAGTCATGCATCTTAAAGATTTTGGAAAAGACA850.1400329489291598No Hit
TGGCTGGGATAGATAGAGAATTACCATTATAACATTGCTCCATTGCCCACTC840.13838550247116968No Hit
GCATACGGGCAGGGCCTGTTACAGTCTACCCCTACATGCATGCCCTGGACAC830.13673805601317957No Hit
GGTAATGGGGACTGATAGCTGTTTTAAGAGCCACTGGCCTGTAATTGTTTGA820.13509060955518945No Hit
TTCACTGGGGTAGCCATGGAAGATACTTTCAAAAGGACATCTTCAGATCATA820.13509060955518945No Hit
CTTGAGACAGGGATGTCATATATGTGTGCTTCATGTCATGCGAAGTATTATC820.13509060955518945No Hit
ATGTTGGGGTCTTTTTGAGGAAGACGCGGTCGTAAGGGCTGAGGATTTTTGG810.13344316309719934No Hit
TTTTTTGGGATTGCACTGCATGGTATCTGCACTCAGCAGTTTACACCTGCTA810.13344316309719934No Hit
ATTCCAGGGAGATGCCTATGGGTGCAGTGCTTGGAGAACCAAATAAAACTAC800.13179571663920922No Hit
TTAACGGGGGCCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCA790.1301482701812191No Hit
CGGGTCGGGGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAG780.12850082372322902No Hit
AAAAAAGGGAATTTTGAGCAGTTACACATTTATCTAAAGGAAATAAATCAAC760.12520593080724876No Hit
CATAATGGGCTGCGCTAATTACCTGAGCGACCAGGACTACATTTCCCAAGAG760.12520593080724876No Hit
GCCACAGGGAATGTTTTAGCAAGCCATTCCTAAACAGCCCAACTGGCATTTA760.12520593080724876No Hit
CGCCTTACCCCCCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACC750.12355848434925865No Hit
TGAGTCGGGAAGATACCATGAATAATTTATGAACTCTGATGCTAAGAAATCC730.12026359143327843No Hit
GACTCCGGGACTTGGGAGAGAAGATCCAGGAGAACAACATCATCTGAGCATG730.12026359143327843No Hit
ATTGCTGGGAATTTCAAAGTCATTTATTGTGGAAGAATGACCAAGAAGTATG720.1186161449752883No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCC710.11696869851729819No Hit
TTTCCTGGGGACTGTTTATAATTCTACAAAGAACAAAAATTGTTATGTACTA710.11696869851729819No Hit
TGCAGCGGGACTGCTGGAAAAGCTAGATGTGAGGGGAAAACAATGGGCATTA700.11532125205930809No Hit
CAAGCCGGGGTTACCATTTTTCTGGATTTAGTAAGAAATTTGCAGTTTTGGT690.11367380560131796No Hit
GTTCCTGGGACTGATCGCATTGAAAACATTGATCACCTGGGTTTCTTTATTT690.11367380560131796No Hit
TATACTGGGATTGCCATCTCTGTTAGTGCAGCCATTAGAAAGACATTGTGCC690.11367380560131796No Hit
CTTTGGTGGGGGCACAAGGGAAGAAGGTGAAAACCTGAGTAGGTATGTTTTG680.11202635914332784No Hit
GTAAAGGGGAGAATGTGAAGATCTCTGATTCTCACAGTAGTCTATTTAGTGT670.11037891268533771No Hit
TGGAACGGGATGATTTCAGAGCACATAAGTAAAGGTGCTTTTTAATGTGCAG670.11037891268533771No Hit
ACCATAGGGTAGAAAAGGAGAAGGAGAGAATCAAGGTGTAAGTTGTCAATGG670.11037891268533771No Hit
TACCGCGGGTCTTAAGCCAAAGGGACATATTTTGGGGTTGCATATTCTGGTT660.10873146622734761No Hit
ACAAAGGGGACTGAATGAAGGAAACATGAATCAAATAAAGTTTTGAATTTTA660.10873146622734761No Hit
CTATATGGGATGGGAAGAGGTCATATCCCTTCCCTGCCCCTCGCTGTCTTTA650.1070840197693575No Hit
GTCATTGGGCTGCCTAAGTTGTCTGCCTTTTCTACCACCAAAAAGACTTTTA630.10378912685337727No Hit
TTAATAGGGGTCCCTTGAAAATGACAAGATTCTGCAGATCATTACGGAATTG620.10214168039538714No Hit
GTTTTTGGGTATCATAAAGGAGCATAAGATGACATTAGGGACAGAGATAGTT610.10049423393739704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)