FastQCFastQC Report
Sat 31 Dec 2022
SRR5849041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296208
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT55071.8591665316264248No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC50371.7004942472856912No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC4100.1384162480419165No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT3230.10904499540863179No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3220.10870739480365148No Hit
GAACTGGGGGGATCTAATTGACTGAAGAGTTTCTGCACAGCAAAAGAAAACT2970.1002673796791444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)