FastQCFastQC Report
Sat 31 Dec 2022
SRR5849037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100939
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT22732.251855080791369No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC22342.213217884068596No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3780.37448359900533984No Hit
CTCGCGTAGGGGTAGAATTACATGAGCCCAGCAGATAGAGGCTGCAGTAAGC3020.29919060026352545No Hit
GTCACGGGGATCTCATCAGGACAGAGCCAGAACAAGGAACCCTCTATTCACC2840.2813580479299379No Hit
GCTGGTGGGAACAACAGTGGAATTAAATTCAAACTTTACAAAAGGAAACATG2820.2793766532262059No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCA2420.2397487591515668No Hit
AGCGTAAGGGGATTAACATTTTTTACAAATTTGCCTGCATAAAGATTAAATT2340.23182318033663896No Hit
GAACATGGGATACAGTTACAGTTTACTCTGCAGAGGTACCTTACCTGGTATT2300.22786039092917507No Hit
GGAAACGGGATTATCACTAAATGGTATATTCTCTTTTCCTGCTGAAAATCTC2290.22686969357730907No Hit
CTTCACGGGCAGATTCACCAAGGTTGAAATGAAGGAAAAAATGTTAAGGGCA2280.22587899622544308No Hit
TAGTGGGGATGGCAGTGGGAGACATAGGAGTAAGTGGAAATGTGTAGGAGAC2260.22389760152171112No Hit
CCGTGGGGACCTGGCTAATTTTTTAAATTTTATGTAGAGGTGGGGTCTTGCT2240.2219162068179792No Hit
TTTACGGGGGGCAGACTGGAGCGTAAAACTTCTGCAAGATGATCAGGACACA2220.21993481211424723No Hit
TGTCGTGGGAGGACGGGAAAGTCCAAGGAAGGGGCCTGGTGGTGCTCCACTT2200.21795341741051524No Hit
CACCAGGGGGAGGTCCCCAAATCCTAAACACTGGCACACCTGGAACTTGCTA2200.21795341741051524No Hit
CACACGGGGAATCCAAAAGTATTTCACAGCACTAATGCTGATCAAAATTTGC2190.21696272005864928No Hit
TTTTCTGGGACAGACTGAGGTGTGTTGCTTTTAATCATAGATAAATTTTAGA2170.21498132535491735No Hit
TCCAGAGGGAGTGTTATAAATATTGAGAAGATACACTGGGGAGATAGCATAT2160.21399062800305135No Hit
GCCGTTGGGATTTAGAATCATATTGGCCGCTTGAACTCTGTAAGCCACCACT2140.2120092332993194No Hit
TCTGCTGGGGACACCTCCCAGGAACCTTCTCCACCCAAGGCATTTGCTGTTA2090.2070557465399895No Hit
GCTTGCGGGCAATAAGTGGTGTAATATGAGTTGTTATGGACTGAATGTTTAT2060.20408365448439156No Hit
GAGTATGAGGCAAAGTGCTAGGATTACAGGCATGAGCCACCACGCTCGGCAT2050.20309295713252556No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCTTATAC2040.20210225978065963No Hit
AAGTTTGGGATTGTATAATATTGGTGGATATGAAATGTGAGTTCTTCTAAAA2000.19813947037319568No Hit
GCAGTTGGGGTAACTCCCATACTTTAAAAATTAAGTATAACATTTAACCTAG1980.19615807566946372No Hit
ATTATGGGAGTCCAAAAAAGTATCTTCTTTTAAGTAAGCAATGAATAAATAT1960.19417668096573176No Hit
ATAGAAGGGGAACTCTTAGTACTGCTGATCGTATCACCCTTTCTTTTCCTTT1940.19219528626199983No Hit
GCTGGGGATAGAGTTCAATATTTTTTAGTTTTTATACTTTTGAGATAAAATT1940.19219528626199983No Hit
GTTTTGGGGATGAGCCAGAAGAAGGGAAAAAAAGAACTGGAAAACAAAATGA1920.19021389155826787No Hit
GGAGTCGGGGCTATATTTTAATGCCATCCCAAAATATCTTGAATTATCTATG1900.1882324968545359No Hit
ATAAGTGGGCCGGGTTAAAGCGATTCTTGTGCCTCGGTCTCCCGGGTAGCTG1880.18625110215080395No Hit
ACTTCGGGGAACTATCCTAAATATATATGCACCCAATACAGGAGCACCCAGA1840.18228831274334004No Hit
TTTCCAGGGAACTCACTTGCTTTTTTTCTCCTCCATGCTAACACGTTCAGCT1830.18129761539147404No Hit
AAGTCAGGGTCCGAGAGGCCAATCACAATGTGTCCGCCCCCTATGGCCGTAT1820.18030691803960808No Hit
GAACTAGGGGTGCGTGCTGATGGCGGTACTGGCGGCTGGCGCCCTGACGCAG1810.1793162206877421No Hit
ATTCGAGGGATCAAATAATATGTTTATTGTCATCTACTGTCCAACACAGGAA1800.17832552333587612No Hit
GCATTGGGATAGCTCTGTATTTCTCACTATTTTCCCCATGATTATTCAGCAT1800.17832552333587612No Hit
ATTCCAGGGACTAGTTTTCTGCTATGATGGCTTTAAATAATTTTACAATTAA1800.17832552333587612No Hit
CTACAAGGGGAGTGATGGCCAGTTACCTTTGATCTTGAGTCTTACTTTCTGA1800.17832552333587612No Hit
GCATTGGGGCTTCCGATCTTGTTGGGGGAGGATTTGCTGGATTTACTAACAA1790.17733482598401012No Hit
ACAGTAGGGGAGCTGTACTTACGTCTAGATGTATAAGAAGCATGGGGTCAGC1760.1743627339284122No Hit
TAGCTCGGGGGTGATTGAGAAAAAAATCATGTTGAGTAAGTTGCCATTGTGA1760.1743627339284122No Hit
TTTGCTGGGGCGATTCTCCTGCCTCAGCCTCCTGGGAGGCTGGGAATACAAA1760.1743627339284122No Hit
AGATAGGGGTCCGAGGCTCTGAGGTCTGTTTTGGCCCAGGGAACTCTAGGAG1740.17238133922468027No Hit
AGCTATGGGGAAAGTGGGGAGATTGACTTAGATGGCTTAGACACACCGTCAG1730.17139064187281428No Hit
TTTCTTGGGACGCCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAACT1720.17039994452094828No Hit
TTGCAAGGGATATCTGTACATTTTCAGAAGTTCCTCTTGTTACTGATTTCTA1710.16940924716908232No Hit
CCGTTTGGGACAGCAGTTCACAAAAAAATGTTCATTGTAGATTTTGTTATGT1700.16841854981721635No Hit
TACCTGGGAGTTGAAGAAACTGAGGCTGCTTGAATTAATAACTCACCCAGCA1660.16445576040975243No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCCTGTCTCT1660.16445576040975243No Hit
ACAATAGGGACAAATCACCTATTTATTAATAAGTTATATTCTAAAAATATTT1650.16346506305788644No Hit
TTCTATGGGGTGTTCTTTATGATAATGATCTTTGTAGATTGGTTATTTCTGT1620.16049297100228851No Hit
GGCGCAGGGTAAGAGTGATGTCTGGTCATATGGAATATTACTGTGGGAAATC1620.16049297100228851No Hit
GAGCAAGGGAGAGACTTCGTCCACGAATCCACACCACCTTTCCCATCAGTTC1600.15851157629855656No Hit
CGTCCAGGGAAAATCTTATAGTTTTCTGAGCTATTACATCAAATCAATTCAT1600.15851157629855656No Hit
TATGCTGGGAGCAGAAACACTGGCAAAAAAACTTTTGAGAGGCAGAGCTTCC1590.15752087894669056No Hit
ACTCGATGGGTCTCCTCTGTGCTTTGTGCTCCAGGAAAAAGCACTGTGTATT1590.15752087894669056No Hit
TAATGGGACGAATGTTCAGAACATGAACCCAATGATGGCCCAGATGCAGATG1590.15752087894669056No Hit
AGGTTTGGGACAGCCATGACCAGCTGTTCACTCATGTTACTATGTCAGATCT1590.15752087894669056No Hit
ATTTCCGGGGGGACGGGGACATGCATCAGTCCTTTGTATACACAAAACTAGT1560.15454878689109264No Hit
TATATAGGGGAGCTGGATGCAGGAGCCCATGGCTGAAAGGAGTTAAAACGCC1550.15355808953922664No Hit
TGCTTGGGAAGAGAACTGCTTGAAATTTCCTGAGAAAATCTGAGTTGTATTA1540.1525673921873607No Hit
TTTCCGGGGGAATGAACAAAAGCTAAACAGATATCCAGCTTCTTCTCTTGTG1530.1515766948354947No Hit
AAGCATGGGAGGGGTTTTTGTTGATAAAATCAAGTCTTGGTTGTGGCTTGCT1520.15058599748362872No Hit
GCTTACGGGGCACTTGTCCCTGGTGACTTCCAGAGTATCTGGCATACCATTG1520.15058599748362872No Hit
ATATCGGGGCGCTCTTCCGATCTATATGTGGGGATTTCTCACAGAGTTAAAG1520.15058599748362872No Hit
GTTCCGGGATAGAATTTGGATCCTTTGGTCGGGTTCTCCCAAATTCTTTTGA1490.1476139054280308No Hit
TCTTTTGGGATGTAGAACATTGTTTGAGCCTTCCTTGTTTGGCATTTTGGCA1490.1476139054280308No Hit
GTTGTCGGGAGACCATGCATTTTTGAAGTTAGTGTCCACTAGGGTCCTATTA1470.14563251072429884No Hit
GAACGGGGGACCTGTGGCGTCGCCAGTGATGGAGCAGATACTCAAGGAGGCA1470.14563251072429884No Hit
TCGCTTGGGAAACTTACAACATCTACTCTTAGGAGATGAGGTGTTTGAAATT1470.14563251072429884No Hit
AAATTTGGGGAAAGTGGCATTCAGCTGACTTGCTCATTTAGACTGTTCACGG1460.14464181337243287No Hit
ATAAAAGGGCTGGAGGTGAGGTATGCCGTCCGCACTCAATAATTACTTGCAG1430.14166972131683492No Hit
TGAACAGGGGTTTTAATTGATAGCAAAATAGTTTATGGGTTTGGAAACTTGC1430.14166972131683492No Hit
GAAACAGGGGGACCAGTATTTGTAATAATCGAGAATGCCTGGGCATTTTACA1410.13968832661310296No Hit
CTAAACGGGTAAGATCACACACACACACACACACACACACACACACGAACTA1410.13968832661310296No Hit
CAGTACGGGATACCAGCAAAAGTGCAGTAGTGTCACTTTTTTCCTGTCAATA1400.138697629261237No Hit
GGAGTTGGGATTCTTTTCAGGAGATCACTGAGAGAGGAATACAAAGCTTAAA1400.138697629261237No Hit
TGAACGGGGAACGTTTCTCAGATAACCTGAAATGTTCATGTGGCATTCATTC1400.138697629261237No Hit
TTTAGAGGGGGCGCAGCCTTGGCTCGCTGTAACCTCCACCTTTCGGGCTCAA1390.137706931909371No Hit
GTAACAGGGACACGAAGGACAAGCATCCATGATTTTTTGACCAAGGACAGTA1380.13671623455750503No Hit
GAAATTGGGGGGACTACAGGAATCATATTGACAGGTCATGAAAATGAGACCA1370.13572553720563904No Hit
GTTCCTTGGGGCTGAAGAAATCCAAGTCCAAGACATATGGAATTAAGCACTC1370.13572553720563904No Hit
ACTCTCGGGTATTCTAGACTCTGATAAACCTTATTTCTCCTAATTCTGTACA1370.13572553720563904No Hit
CAAAGGGGAGCCAGCCCGGGGATGATTGATGTCCGGAAAAACCCCCTGTAAG1360.13473483985377308No Hit
TGTATTGGGACCTCTGTTCAGAATATATACCATATGTCTGTTCTGTGGTTCC1360.13473483985377308No Hit
GAACCTGGGATGCATGAATGATCTTAAATTTCCTGTAGTTGTGCTGGATGAG1350.13374414250190708No Hit
GCTGATGGGATTTCATAGTGCTTAAGATGTTAACTATATCGAGATCATTAAT1340.13275344515004112No Hit
ATACAGGGGGAATTAGCTAGAACTTGTAGTTTGCATAGTATCCGACAGATGT1340.13275344515004112No Hit
GCCACAGGGAACACAAACCTCAAGCTTTACTGACTTCAATTATCTGTTTTTA1330.13176274779817515No Hit
TCTAAAGGGGTGGTGGTCCTCAGCTTTGGGTGGATGTTCAAAAAATCTGAGC1300.1287906557425772No Hit
TGATTAGGGAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGA1300.1287906557425772No Hit
TCATGAGGGTGCCAAAGGCTTTCCTAGGCAAAGTCATTCTGTGAAGACTGGA1300.1287906557425772No Hit
AGACGGGAACCAGTTTGTGTTTATTTTGTTTTCTAATAGCAGTGGGAACTAA1290.12779995839071123No Hit
GTAACTGGGACTAACAGTAGAAATTAAAACAAATTTCTAAAATGACTTTTCT1290.12779995839071123No Hit
TTTGCTGGGAAAAGGAAATAGTAGCATAGTTAATTGTGTTTTTGTATGTCCA1270.12581856368697925No Hit
TTGCAGGGGAAGTATTGATATAGCATTTACATTGTATTAGGTATTATAAGTA1270.12581856368697925No Hit
GTTATCGGGGTTGACAATGTCTTGTGGAGCATTTTTGCTGAGGAAAAGGTCA1270.12581856368697925No Hit
ACTTCAGGGAGTGTCTGTCAGCCCTCTTAACTCTGTGCACGCCCTATTTCAG1260.1248278663351133No Hit
TGTTGAGGGCAGTGCTCAAGAACAATTTGTGTCCCTCGGGCAGCAACATCAT1250.12383716898324731No Hit
GGTTCCGGGAATGACTTAATGCTTCAAAAAACATCAACAAATTTAAAAGAAA1240.12284647163138132No Hit
GTAGTGGGGCTGCGGGGGCCGCTTCCACCCTCAGCCGATGATAATCTCAAGA1240.12284647163138132No Hit
CTCTTTTTGGGGAAAAAAGACAACTCAGAATGTTGTTATTTGAGCATAATTC1230.12185577427951536No Hit
GAAAAGGGGATTTTTATTAACTTTTAGTTAAATACAGATTTTACAACGAGGT1220.12086507692764938No Hit
AAGCTGGGGGACTCACCCTCTCTCAGCCTCAGTTTCCTCATTTCTAAATTGA1220.12086507692764938No Hit
CTACTAGGGGAAACTGAGACCAAGTCTCTCTAAGACAAATGCAGTGTATTTA1220.12086507692764938No Hit
GGTTATGGGACAGACAATGGATTTTATAAATATTTAACATTTTTGCCAATGT1220.12086507692764938No Hit
TCTAGAGGGCCAAATGGGATATGGCCAATTTTCCAATAACATAAGAAAAGAT1210.1198743795757834No Hit
TGCTAGGGGGGACAAACTGAGCTTACTGAAAGTCTGAAGACCAAAGCTTTTC1210.1198743795757834No Hit
TCATAGGGGGAGTTTCCTTTCTCACAGTTTTCCATCTCCCCACTTCTTTGAT1180.11690228752018546No Hit
GGCTCAGGATAGCATTAACATGTCATCAGATAATTTCCCGCAGACAAGTGGT1180.11690228752018546No Hit
AATTTAGGGAACACTATTTTGGCAGTTTACACATTACTTTTTGTTTTTCCTT1170.11591159016831948No Hit
GTGAAAGGGCCGATCTATTGATGGGGTATTATATAAAATATGTATGTTTTAT1170.11591159016831948No Hit
GTACCAGGGATGTAAAACCATTAAAATACATTGACATCCCAACACATATTTT1160.11492089281645351No Hit
GGCGTTGGGCTAGAAGTGGAAGGATTGTAGTCATCCTGAAAATGGGTTTACT1160.11492089281645351No Hit
ACAACAGGGTGGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATTG1160.11492089281645351No Hit
GATGGTGGGATTGCATGAATCCAAGGCCAGAGTGCTATGAAGTGGGGGTTAT1160.11492089281645351No Hit
CTAGCCGGGATTTTCTAAGAAGAAGAATAGATCATAAATCTGACAAGGAAAA1150.11393019546458753No Hit
TAAGTGAGGGGGCAGAGAGAAAGAGAGAGAGGGAGAGGAAAGAAGTGGGTGT1150.11393019546458753No Hit
TATACCGGGAAATAATCCATACCTTGAAGAAGTTGGCCAATGATATTATATA1140.11293949811272154No Hit
TTGGATGGGCGGGTTCGGGTGATTCTCCTGCCTCAGCCTCCCGAGTGGCGGG1140.11293949811272154No Hit
TCGCGAGGGGTTTCCTCTATTTGTAGCTTAATTTTCTTCATAGAACATAACA1140.11293949811272154No Hit
AGTGAAGGGGTCATATGTCTGTGATATCTCCAAATAGTAAAATAAAATTTAA1140.11293949811272154No Hit
CACAAGGGGAAATAATGTTACAAATACATTTATTGACATGTAATTCATTATG1130.11194880076085556No Hit
TCCATAGGGGTGTAGGTGAATGCAAACTCCATCGTTGAGCCTGGGGTGTAAG1130.11194880076085556No Hit
GAAGCGGGGACTTGCTCCAATCTGTATTCTGTACAGAATTGTATATCAAAGA1120.1109581034089896No Hit
TAAAAGGGAGCTGGGGGGATTGGGATACTTTACAATTTTTACATTTTTAAAT1120.1109581034089896No Hit
TGACTAGGGATGTAATTGACTTGTAACTATGCCCGTGGTGTAGGAATTAATG1120.1109581034089896No Hit
AAATAAGGGATGTCGGAGGTGCTGCCCTACGGCGACGAGAAGCTGAGCCCCT1120.1109581034089896No Hit
GCGTAGGGGGTACAATCTTGCTATGGTTCTGTATCCTTGGCCTTCACAGTTG1120.1109581034089896No Hit
CTTTACGGGCGGTCACACCTCTGCAGGGCCGGCTCTCTGATAGAAAGTGGAA1120.1109581034089896No Hit
TATGGCGGGGGAATGACTCCTTTTCCTTCCTGGCCCTCGGGGGCCTCTCGAG1110.10996740605712362No Hit
CTTTTTGGGGAAGTCCCTTGAGCACAAAAATTTAAAATTTTGTAAATCTAGT1110.10996740605712362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAACTCGTATGC1100.10897670870525762No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCAT1100.10897670870525762No Hit
CCAACAGGACTGAAACCAGATATTTATGCAACAATCATGGACTTCTTTGCAT1100.10897670870525762No Hit
ATTGATGGGATTACAGCTAAAAAAATCATTCATTAGCAAAAGGAAAAGTGGT1100.10897670870525762No Hit
TCGAATAGGGGGAATTGATTGGGATAATATTCGGAACTGTGAAGCACCTTAT1090.10798601135339166No Hit
ACCTAAGGGAATTATAGTTCCCATAATCCCCACTTGTCATGGGAGGGAGCAG1090.10798601135339166No Hit
TATCAGGGGACAGGAGACAGGAAGATAAGAGAGAAATGTGGAAGATTAGAAG1090.10798601135339166No Hit
AGACCCGGGGGTGTCCCAGCAGCCTGTCCTGTTGGTTCAGATAATGATTTAT1090.10798601135339166No Hit
TTGAATGGGAAAGAATACAAGAGGATGTGCATAGGTTATGTGAAAATACTAC1080.10699531400152568No Hit
TACGTGGGGAGATATTTACCTGATACTAATCAGGAAATATATGATATCCGTA1080.10699531400152568No Hit
CACAAAGGGCATTACAGAGACTCAGCCAGGAGGACATGCCCTGGGGAGTTTA1080.10699531400152568No Hit
AGCACAGGGTGGAGAATGGGGTGTTGCCGTGTTGGCCAGGCTGGTCTCGAGC1080.10699531400152568No Hit
CATGTGGGGGATCTCTCGCGGGTACATTACACTAGTAGAATTAAGGGAGAAT1070.1060046166496597No Hit
CAATTAGGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT1070.1060046166496597No Hit
TTCGAGGGGAGCAAGACTAATAAAGAAGAAAAGAGAGAAGGATCAAATAGAC1070.1060046166496597No Hit
CTTAAGGGGGGTGGAGGCCATGCCCTGTATCTTTCAGGACCTGTTTTAGTTG1060.1050139192977937No Hit
TGATATGGGACTGGCATCACTAGATGGACAATCCAAGAAGAAATTGGTATAC1060.1050139192977937No Hit
CAACAGGGGATTCCAAAACAGCTTTAATGGCCCATATGTACACTTCGTAATC1050.10402322194592775No Hit
GCTACTGGGACAGAATGAAACATTAATGGTAAAAGTGGAGTAATTATTTAAA1050.10402322194592775No Hit
AACGCAGGGAACCATCAGCATGCTTTGCTCAAGTACTTCATATTCAGTAGGA1050.10402322194592775No Hit
AGGTTAGGGAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCA1050.10402322194592775No Hit
AAATTTGGGACCTTGAGCTGTATATCCTTTTAGTTTTTAATGTGTATGTGTA1040.10303252459406176No Hit
TTTAAAGGGTCACCCAGCGAGTGCTGAGACCCCATTTTCTGTCGAGGCGGGC1040.10303252459406176No Hit
TTCGTAGGGGGCTCACTGCAACCTCTGCCTCCTGAGTTCAAGTGATTCTCCT1030.10204182724219578No Hit
CTACGCGGGGTGGGAAAGACTATAGGCAATAGGAATCTCCTGCAAACTCCTA1030.10204182724219578No Hit
ATCTGAGGGGGTAGCGCACTGCTTCGAGAACGCCCACCGCGTGCGGGTGTCC1020.10105112989032981No Hit
GGTCCCGGGAGGAACCAAGAAATGAATGGCACATGAGTGGAACAGGCATATT1020.10105112989032981No Hit
CTTTAAGGGGTTATCAACCATATGTACATGAGAACCCTTCCCCTTCATGGCG1010.10006043253846383No Hit
ACACTAGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA1010.10006043253846383No Hit
TCACACGGGGGGTCTGCACCACCTTTAAGAGCTGCAACACTCACCGCGAGGG1010.10006043253846383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)