FastQCFastQC Report
Sat 31 Dec 2022
SRR5849035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309414
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT29620.9572934644198388No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC27700.8952406807707474No Hit
TTTTTAGGGATATTTCTTAATTTTAAAATAATTTGCCATTTTCTAGTTAGCT4740.15319280963369467No Hit
TATACTGGGGACTACCACAATGTGTACCGAAAGAGTTAGGTAGCTCAAGAGG4160.13444769790636493No Hit
GAGCAAGGGGACCGGCGGACTCGTGAGCGCGCCGCTGCCGGGACCGCTCCTG4060.13121578209130808No Hit
AAATTCGGGGATGCATTGACGTGACCTGAACTAATTCGGAAAAGTATTCTAA3930.12701429153173419No Hit
CTATTAGGGATCCTTGCCTTGTTGAACTCAGTTTGCTAGTATTTTGTTGAGG3930.12701429153173419No Hit
CTGATAGGGAACTAATAAGCTCTTAACAGGCACTTTATTGTGGGGAGGGGAA3900.12604471678721713No Hit
TAAACTGGGAGCAGTGTCTACTCGTAGTGAATTCAGTCACTTTTGTATTTTC3890.12572152520571145No Hit
GATTTTGGGAGTTAGAATAAGTTCTCATTTCCAATCTGTAACTTGGATTCAC3880.12539833362420577No Hit
GTTCAGGGGGCAAGCCAGCAAGATAACTTCACCAGGGAATTGAATTAGCCAG3750.12119684306463184No Hit
CTCGTTGGGATTTCCTACTCAGTCTGAGTCGGAGATCTCACCCTAGTGTGTG3740.12087365148312616No Hit
ACTGGTGGGCCGATCTTAGGGGGGAGTCAACCATCCCATGTGGTGACTGATA3720.1202272683201148No Hit
CTTGAGTTGGGATGATTAACTTTTTCTATTTGTTATAGCCCTTAGAAAGTGT3700.11958088515710343No Hit
TGACGCGGGATATATGTGGAAAATTCTTCTCTCAGATGTCATACCTCACTAT3690.11925769357559773No Hit
CAAATCGGGGATCATAACACAAGAACAACAACATTCAAAGATCCTCGCAATG3690.11925769357559773No Hit
GACCTTGGGGAGAAGAGCGGCAGTTTCGTGAGCGACCTCAGCAGTTACTGCA3670.11861131041258637No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3670.11861131041258637No Hit
GCATATGGGAGGCCCCCATGATTCAGTGATCTTCCCTTGGGTCCCTTCCACA3660.11828811883108069No Hit
TTATAGGGAGCTGGAGAATTGAAATCACTACATCACAAATATTCCATCACTT3650.117964927249575No Hit
GCTACGGGTAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT3640.11764173566806932No Hit
ACGGGGATGAAGAGCAGAGTTTGAAGACAGAGACAGTCAGGTGGTGTCAGTT3590.11602577776054089No Hit
AGGGTAGGGCACAACATTATTCACAATGGCCAAGATATGGAATCAATGTAAT3590.11602577776054089No Hit
CCGCTAGGGGGGGCCGGGGCCGAGGCCGCGGAGCTCGCGGAGGCAAGGCCGA3560.11505620301602384No Hit
TGGCGCCGGTCCCACCAGCAACGCACACGGACTGCAATTCCTCCACACCCTT3540.11440981985301248No Hit
CACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAA3540.11440981985301248No Hit
GCGCTTGGGGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAAGGA3530.11408662827150678No Hit
TTTGCTGGGAATGACAAGGAAAAAAAAGCCTGTACATGTTCAGCACAAGTGA3510.11344024510849542No Hit
AAATACGGGAATATAGAAATAGTGAAAAAAATTGTTATTAAAGTCTCTTCAG3510.11344024510849542No Hit
GCGAATGGGAGCAATGAAGAGGAGGAAGAGGAAGATGAAGAAGAGTCAGAGT3470.11214747878247268No Hit
GATAGTGGGAATGACATAGTCTGTGTCTAAGCCGCCTTTATTTCAAAGGACT3460.111824287200967No Hit
TAACACGGACAGGTTATCCTGATTCATATTTCTTTGAAGGTAGTCTTGGGAA3450.11150109561946131No Hit
TTCAAAGGGTTACAGTTTATACTAAAACATTAAGAATCAATTATTATAAGAG3450.11150109561946131No Hit
TAATTAGGGGCATTAAATATGGAATATCTTCTTACGGAATATCTTCTTCCTT3450.11150109561946131No Hit
TCGACAGGGAGGCCAGCTGCCGCCTCCCCCACTATGTAGCATCTGATTCGTC3440.11117790403795563No Hit
ATGAAAGGGGGCTGGAGCGGGAACGGGAGTAGCTGCCTGGGCGCCAAAGGCC3440.11117790403795563No Hit
CATGAGGGGGTGCCCCGAGGTGAGGCGGCGGGGGCGGTGCAGGAGCTGGCGC3420.11053152087494426No Hit
TGTAGTGGGAGGCCAGTTCTGGAACTTCACTGAGTCCCTCTGCTCTTCTGAA3420.11053152087494426No Hit
TTGAGTGGGGACTTTGGTTCTGGCAGAGGGAAGCGACCCTGATGAGGGTGTC3420.11053152087494426No Hit
GCTATTGGGATTCTAAGGGCAAGTGTAACCTACAGTTGTTGGATTTCTTTGG3420.11053152087494426No Hit
CAGTACGGGAGGAAAAATGAAATTTAGCAATTTGTTTTCATTTAATTTTATT3400.1098851377119329No Hit
CTAACCGGGGTTCTTGTTTTGTTTTGTTTACCCACTGGGAATCACTTTCCCA3380.1092387545489215No Hit
TGCTCTGGGGTCGTGTGCCATGACATTTCTATTGCACATTCTTTGTACTGGC3330.10762279664139308No Hit
AGATCTGGGAGATATACATATGTCTTACCTGTTAGTGCATGGAATTCAAAGT3300.10665322189687602No Hit
GCATAAGGGGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCT3260.10536045557085329No Hit
CTTTACCGGGGGTTAATGTAAAACTTACCAGATGAACCTTGTGTTTCAGCTT3250.10503726398934761No Hit
CTATGAGCGGGACTAATAGAATCAATATAATTGAAAATTGATATTTAAAAAT3240.10471407240784192No Hit
TTTGCTGGGGACATCACTATAGATGTGAGAGGCATTAAACAATCATAAGGGA3220.10406768924483055No Hit
GACGATGGGGCTTCCAAAAGTGATAGTGTGTGTAAGATTTTTACCTTCCTTT3210.10374449766332487No Hit
GTAAATGGGTAGCTCACTGCGGCCCCAAACTTCTGGGCTCAAGTGGTCTTAC3200.10342130608181918No Hit
AAGTCAGGAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAAT3200.10342130608181918No Hit
AGTGCAGGGCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGA3190.1030981145003135No Hit
TAATGGGGAGCTGGGTGAAGTGGCAGCCTTTGGGGGCAGCAATGTGGAGCCC3190.1030981145003135No Hit
TTGGATGGGAGGGTCAAAAGACAGTTACTAGCAATGGGGAATGCTTGTCACT3150.10180534817429077No Hit
TCAATTGGGCAGTAAAGAACTGTGTATTGTGGCTTGGGTTATAATCACTTGT3120.1008357734297737No Hit
TTTGCTGGGGTACTTGGAGTGGGGCCTCAGAATTTCTATTTCTGAAAAGTTC3110.10051258184826803No Hit
TGCAATGGGAGAACTGAAGCAAAAAGGTTTCAATCCGTTCCGTTTTCTTTGT3110.10051258184826803No Hit
CTTACAGGGACCCTTCATCCAGCCCACTCTGGGAAGACTGCGAGAGTTGCAA3110.10051258184826803No Hit
TATGCGGGGTACGAAGTTATCAATGCATCTTTCAGTATAGGCAGATCTCTCC3110.10051258184826803No Hit
TGTTCAGGGAGGCGTGAGCCACCACGCCTGGCCTGGTTAAAGACTTGAGTGA3100.10018939026676234No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)