FastQCFastQC Report
Sat 31 Dec 2022
SRR5849034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308022
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT30440.9882410996617125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC28390.9216874119381083No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC4320.14024972242242437No Hit
TTTTTAGGGATATTTCTTAATTTTAAAATAATTTGCCATTTTCTAGTTAGCT4300.13960041815195018No Hit
CAAATCGGGGATCATAACACAAGAACAACAACATTCAAAGATCCTCGCAATG4200.13635389679957927No Hit
AAATTCGGGGATGCATTGACGTGACCTGAACTAATTCGGAAAAGTATTCTAA4140.1344059839881567No Hit
GATTTTGGGAGTTAGAATAAGTTCTCATTTCCAATCTGTAACTTGGATTCAC4100.13310737544720833No Hit
TGACGCGGGATATATGTGGAAAATTCTTCTCTCAGATGTCATACCTCACTAT4090.13278272331197122No Hit
GTTCAGGGGGCAAGCCAGCAAGATAACTTCACCAGGGAATTGAATTAGCCAG4090.13278272331197122No Hit
TAACACGGACAGGTTATCCTGATTCATATTTCTTTGAAGGTAGTCTTGGGAA3970.12888689768912612No Hit
GACCTTGGGGAGAAGAGCGGCAGTTTCGTGAGCGACCTCAGCAGTTACTGCA3900.12661433274246645No Hit
GATAGTGGGAATGACATAGTCTGTGTCTAAGCCGCCTTTATTTCAAAGGACT3860.12531572420151807No Hit
TTATAGGGAGCTGGAGAATTGAAATCACTACATCACAAATATTCCATCACTT3760.12206920284914713No Hit
GAGCAAGGGGACCGGCGGACTCGTGAGCGCGCCGCTGCCGGGACCGCTCCTG3740.12141989857867294No Hit
GCGCTTGGGGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAAGGA3710.12044594217296167No Hit
ACGGGGATGAAGAGCAGAGTTTGAAGACAGAGACAGTCAGGTGGTGTCAGTT3700.12012129003772458No Hit
ATGAAAGGGGGCTGGAGCGGGAACGGGAGTAGCTGCCTGGGCGCCAAAGGCC3690.11979663790248749No Hit
TATACTGGGGACTACCACAATGTGTACCGAAAGAGTTAGGTAGCTCAAGAGG3650.11849802936153911No Hit
CTAACCGGGGTTCTTGTTTTGTTTTGTTTACCCACTGGGAATCACTTTCCCA3650.11849802936153911No Hit
CACTTGGGGTATGGTATCTACCAGAGATACCAACTAGAAGAATTTCTTTTCA3580.11622546441487945No Hit
GCTAATGGGTATGACATTGTGATATCAGTGTTTGACTCTCATTGTGTATGTT3580.11622546441487945No Hit
CTATGAGCGGGACTAATAGAATCAATATAATTGAAAATTGATATTTAAAAAT3570.11590081227964236No Hit
TTCAAAGGGTTACAGTTTATACTAAAACATTAAGAATCAATTATTATAAGAG3570.11590081227964236No Hit
AGATCTGGGAGATATACATATGTCTTACCTGTTAGTGCATGGAATTCAAAGT3530.114602203738694No Hit
GCTACGGGTAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT3510.1139528994682198No Hit
ACTGGTGGGCCGATCTTAGGGGGGAGTCAACCATCCCATGTGGTGACTGATA3500.11362824733298271No Hit
AGGGTAGGGCACAACATTATTCACAATGGCCAAGATATGGAATCAATGTAAT3490.11330359519774562No Hit
TTGCGGGGTCTGGCTCCTACATCGCCCCTGGCACTGAAGAGGAGGGAGAGGC3480.11297894306250852No Hit
AAATACGGGAATATAGAAATAGTGAAAAAAATTGTTATTAAAGTCTCTTCAG3480.11297894306250852No Hit
CTCGTTGGGATTTCCTACTCAGTCTGAGTCGGAGATCTCACCCTAGTGTGTG3470.11265429092727143No Hit
ATTTTGGGGTGAAAACTTAAGCTGAAAAATTGTAAAAACATTTCACGCCTAC3460.11232963879203434No Hit
CTGATAGGGAACTAATAAGCTCTTAACAGGCACTTTATTGTGGGGAGGGGAA3460.11232963879203434No Hit
TTTGCTGGGGTACTTGGAGTGGGGCCTCAGAATTTCTATTTCTGAAAAGTTC3430.11135568238632305No Hit
AATGCTGGGGATTCATGAGTGTTAGCTTTTTGGCGTGTTCTGAATGCCAACT3430.11135568238632305No Hit
CTTGAGTTGGGATGATTAACTTTTTCTATTTGTTATAGCCCTTAGAAAGTGT3410.11070637811584888No Hit
CCGCTAGGGGGGGCCGGGGCCGAGGCCGCGGAGCTCGCGGAGGCAAGGCCGA3400.11038172598061179No Hit
GCTATTGGGATTCTAAGGGCAAGTGTAACCTACAGTTGTTGGATTTCTTTGG3390.11005707384537469No Hit
CTCCAGGGGAGTTTTTTATAACCATAGTTGATTAGGCCAAGAGGCATTCATT3380.1097324217101376No Hit
CTATTAGGGATCCTTGCCTTGTTGAACTCAGTTTGCTAGTATTTTGTTGAGG3370.1094077695749005No Hit
AACAAAGGGGCTTCTTGAAGTTGTTCAACTATCCTTGCCACTGGAAGACCAA3360.10908311743966341No Hit
GTTATAGGGATTTCTCTGTTCCATAAAATGAAGAAACAAAAAATCTTCTTAC3350.10875846530442632No Hit
TAATGGGGAGCTGGGTGAAGTGGCAGCCTTTGGGGGCAGCAATGTGGAGCCC3340.10843381316918922No Hit
CATGAGGGGGTGCCCCGAGGTGAGGCGGCGGGGGCGGTGCAGGAGCTGGCGC3330.10810916103395213No Hit
TAACATGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA3330.10810916103395213No Hit
ATCCTGGGGGATTGTTGGGGTCTCTTTTCTGTACCTGATGTTGGTTGCACAA3310.10745985676347794No Hit
TAAACTGGGAGCAGTGTCTACTCGTAGTGAATTCAGTCACTTTTGTATTTTC3300.10713520462824085No Hit
TAATCAGGGATGATTGCTTTGTTTTGGATCCATTAATTCCAATGTAGACTAT3290.10681055249300375No Hit
TGTAGTGGGAGGCCAGTTCTGGAACTTCACTGAGTCCCTCTGCTCTTCTGAA3280.10648590035776666No Hit
CACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAA3280.10648590035776666No Hit
TTTGCTGGGGACATCACTATAGATGTGAGAGGCATTAAACAATCATAAGGGA3270.10616124822252956No Hit
TTTGCTGGGAATGACAAGGAAAAAAAAGCCTGTACATGTTCAGCACAAGTGA3270.10616124822252956No Hit
TGCAATGGGAGAACTGAAGCAAAAAGGTTTCAATCCGTTCCGTTTTCTTTGT3240.1051872918168183No Hit
AAGTTAGGGAACAACCTCAATCACATCTAAACTCCTGAGTACACACATCATC3240.1051872918168183No Hit
CAGTACGGGAGGAAAAATGAAATTTAGCAATTTGTTTTCATTTAATTTTATT3230.1048626396815812No Hit
GCGAATGGGAGCAATGAAGAGGAGGAAGAGGAAGATGAAGAAGAGTCAGAGT3230.1048626396815812No Hit
TAATTAGGGGCATTAAATATGGAATATCTTCTTACGGAATATCTTCTTCCTT3230.1048626396815812No Hit
AAGTCAGGAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAAT3220.10453798754634408No Hit
TGTTCAGGGAGGCGTGAGCCACCACGCCTGGCCTGGTTAAAGACTTGAGTGA3210.10421333541110699No Hit
TGCTCTGGGGTCGTGTGCCATGACATTTCTATTGCACATTCTTTGTACTGGC3210.10421333541110699No Hit
CTCATAGGGAACCAGTTTAGCAGAAAGTATAAAGTCAATGAACCTTAGGCCA3210.10421333541110699No Hit
GTAAATGGGTAGCTCACTGCGGCCCCAAACTTCTGGGCTCAAGTGGTCTTAC3200.10388868327586989No Hit
TGGCGCCGGTCCCACCAGCAACGCACACGGACTGCAATTCCTCCACACCCTT3190.1035640311406328No Hit
TTGCATGGGCAGGATTTGGTAATCTCATGTCTAAGCTGTGGTAGATTTGAAC3170.10291472687015861No Hit
GAACCTGGATGACATTTCAGTTAACTAGTATTACCTGTTTGTAGAATCTCTG3170.10291472687015861No Hit
GCATAAGGGGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCT3160.10259007473492152No Hit
AGCACTGGGAAATATGCCAGGCTCAGAATCATAGAACTGTCATATCATAAAC3150.10226542259968444No Hit
TGCTTAGGGAGAATACAGAAATCTATAGAGACAGAAACTAGATTAATGGTTG3150.10226542259968444No Hit
CAGTCGGGAGTCACCCTTTTTAATCTCTTTGGTAGTCAGAAGATATTATCAG3140.10194077046444734No Hit
TAGCCTGGGACGTCAAGGAGCTTACAGTCAAGTCATACATAAATTTGTCTGT3140.10194077046444734No Hit
CTTTACCGGGGGTTAATGTAAAACTTACCAGATGAACCTTGTGTTTCAGCTT3130.10161611832921025No Hit
TGATGCGGGAAATATTTTACTGTAAGAATACTATTTCTATACTCTGTTCCTA3130.10161611832921025No Hit
GCATATGGGAGGCCCCCATGATTCAGTGATCTTCCCTTGGGTCCCTTCCACA3120.10129146619397315No Hit
TGGTACGGGACCACCGTGTAGCTTCTAACAGTTGATCCTCGTACTGTTTGTT3110.10096681405873606No Hit
GCCCAGGAGTAATGGAGTCCAAAGAGGAACGAGCGTTAAACAATCTCATCGT3090.10031750978826187No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)