FastQCFastQC Report
Sat 31 Dec 2022
SRR5849033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308232
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGCCGTCT28610.9281969425627449No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGCATCTCGTATGC27080.8785590075008435No Hit
GCGCTTGGGGAAAAACTGAATCATTTAAAATCACAAAAGAGAAAATCAAGGA4330.1404786005346622No Hit
GATTTTGGGAGTTAGAATAAGTTCTCATTTCCAATCTGTAACTTGGATTCAC4320.1401541695865452No Hit
TATACTGGGGACTACCACAATGTGTACCGAAAGAGTTAGGTAGCTCAAGAGG4260.13820758389784318No Hit
TTTTTAGGGATATTTCTTAATTTTAAAATAATTTGCCATTTTCTAGTTAGCT4220.13690986010537518No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCTCTGC3880.12587920786939708No Hit
AAATTCGGGGATGCATTGACGTGACCTGAACTAATTCGGAAAAGTATTCTAA3870.1255547769212801No Hit
GAGCAAGGGGACCGGCGGACTCGTGAGCGCGCCGCTGCCGGGACCGCTCCTG3860.12523034597316307No Hit
CAAATCGGGGATCATAACACAAGAACAACAACATTCAAAGATCCTCGCAATG3780.12263489838822705No Hit
AGATCTGGGAGATATACATATGTCTTACCTGTTAGTGCATGGAATTCAAAGT3680.11939058890705703No Hit
GTTCAGGGGGCAAGCCAGCAAGATAACTTCACCAGGGAATTGAATTAGCCAG3680.11939058890705703No Hit
TGACGCGGGATATATGTGGAAAATTCTTCTCTCAGATGTCATACCTCACTAT3670.11906615795894002No Hit
GCGAATGGGAGCAATGAAGAGGAGGAAGAGGAAGATGAAGAAGAGTCAGAGT3650.11841729606270601No Hit
TTTGCTGGGAATGACAAGGAAAAAAAAGCCTGTACATGTTCAGCACAAGTGA3640.11809286511458902No Hit
CTATTAGGGATCCTTGCCTTGTTGAACTCAGTTTGCTAGTATTTTGTTGAGG3620.117444003218355No Hit
ACTGGTGGGCCGATCTTAGGGGGGAGTCAACCATCCCATGTGGTGACTGATA3610.117119572270238No Hit
CTCGTTGGGATTTCCTACTCAGTCTGAGTCGGAGATCTCACCCTAGTGTGTG3580.11614627942588698No Hit
ACGGGGATGAAGAGCAGAGTTTGAAGACAGAGACAGTCAGGTGGTGTCAGTT3570.11582184847777No Hit
GATAGTGGGAATGACATAGTCTGTGTCTAAGCCGCCTTTATTTCAAAGGACT3570.11582184847777No Hit
ATTTTGGGGTGAAAACTTAAGCTGAAAAATTGTAAAAACATTTCACGCCTAC3560.115497417529653No Hit
TAGCCTGGGACGTCAAGGAGCTTACAGTCAAGTCATACATAAATTTGTCTGT3560.115497417529653No Hit
TTCAAAGGGTTACAGTTTATACTAAAACATTAAGAATCAATTATTATAAGAG3540.11484855563341897No Hit
GAGTTGGGGAAAGATTGGATTGTCTAGCCATTCATCAAATGCTTAAAATTTG3530.11452412468530197No Hit
TTTGCTGGGGTACTTGGAGTGGGGCCTCAGAATTTCTATTTCTGAAAAGTTC3500.11355083184095097No Hit
TAACACGGACAGGTTATCCTGATTCATATTTCTTTGAAGGTAGTCTTGGGAA3490.11322640089283396No Hit
GACCTTGGGGAGAAGAGCGGCAGTTTCGTGAGCGACCTCAGCAGTTACTGCA3490.11322640089283396No Hit
GCTACGGGTAAGAATATCTTGAATGTTGTGTATATATTTTAAAAAGCACTTT3490.11322640089283396No Hit
AAATACGGGAATATAGAAATAGTGAAAAAAATTGTTATTAAAGTCTCTTCAG3470.11257753899659997No Hit
CAGTCGGGAGTCACCCTTTTTAATCTCTTTGGTAGTCAGAAGATATTATCAG3460.11225310804848297No Hit
TTATAGGGAGCTGGAGAATTGAAATCACTACATCACAAATATTCCATCACTT3450.11192867710036596No Hit
TATAGAGGGATATTATTTTTGTGAATCATGTGAGTGTTGGTTGTAGACATCA3440.11160424615224895No Hit
GCTATTGGGATTCTAAGGGCAAGTGTAACCTACAGTTGTTGGATTTCTTTGG3440.11160424615224895No Hit
CACTTGGGGTATGGTATCTACCAGAGATACCAACTAGAAGAATTTCTTTTCA3420.11095538425601496No Hit
TAATTAGGGGCATTAAATATGGAATATCTTCTTACGGAATATCTTCTTCCTT3370.10933322951542995No Hit
TTTGCTGGGGACATCACTATAGATGTGAGAGGCATTAAACAATCATAAGGGA3360.10900879856731292No Hit
TGCAATGGGAGAACTGAAGCAAAAAGGTTTCAATCCGTTCCGTTTTCTTTGT3360.10900879856731292No Hit
AAGTTAGGGAACAACCTCAATCACATCTAAACTCCTGAGTACACACATCATC3330.10803550572296193No Hit
CTACGAGAGGGTTCTCTGGAGAGAAATACAACCTGCACATGTGGTGAGGCAG3300.10706221287861092No Hit
CCGCTAGGGGGGGCCGGGGCCGAGGCCGCGGAGCTCGCGGAGGCAAGGCCGA3290.10673778193049392No Hit
CTGATAGGGAACTAATAAGCTCTTAACAGGCACTTTATTGTGGGGAGGGGAA3290.10673778193049392No Hit
TGTAGTGGGAGGCCAGTTCTGGAACTTCACTGAGTCCCTCTGCTCTTCTGAA3280.1064133509823769No Hit
AGGGTAGGGCACAACATTATTCACAATGGCCAAGATATGGAATCAATGTAAT3280.1064133509823769No Hit
TGCTCTGGGGTCGTGTGCCATGACATTTCTATTGCACATTCTTTGTACTGGC3270.1060889200342599No Hit
CTATGAGCGGGACTAATAGAATCAATATAATTGAAAATTGATATTTAAAAAT3260.1057644890861429No Hit
ATCCTGGGGGATTGTTGGGGTCTCTTTTCTGTACCTGATGTTGGTTGCACAA3260.1057644890861429No Hit
GCATATGGGAGGCCCCCATGATTCAGTGATCTTCCCTTGGGTCCCTTCCACA3260.1057644890861429No Hit
TCGACAGGGAGGCCAGCTGCCGCCTCCCCCACTATGTAGCATCTGATTCGTC3250.10544005813802591No Hit
AAACCCGGGGCCAGAGAAAAATGAACATTCCTATTGCACAATGATCCGAGGA3230.1047911962417919No Hit
TATGTAGGGGACCGAAAACAAGTTGTGCTAATGTCTGTTAGACTTTTCAGTA3220.10446676529367489No Hit
CTTGAGTTGGGATGATTAACTTTTTCTATTTGTTATAGCCCTTAGAAAGTGT3220.10446676529367489No Hit
TGCTTGGGGATCTTAAATGGCTACCTAAATTGAAATCCTTTTCAGAAAAAAT3210.10414233434555789No Hit
GCATAAGGGGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCT3170.10284461055308988No Hit
TAAACTGGGAGCAGTGTCTACTCGTAGTGAATTCAGTCACTTTTGTATTTTC3160.10252017960497288No Hit
AACAAAGGGGCTTCTTGAAGTTGTTCAACTATCCTTGCCACTGGAAGACCAA3150.10219574865685586No Hit
TTCCAGGGGATCCCACCAAAGCTTATGCTTTGATCTTTAGGTGGAATTGCTT3140.10187131770873886No Hit
ACTCTAGGGGCAGTGATGATGACTTCTGAAACAAAATTTGGATTTCCTTTTA3140.10187131770873886No Hit
AAATCTGGGGGGAATATGTAGAGGAGAACCTGGATGTACTTAAGAGTGGCAT3140.10187131770873886No Hit
TAATGGGGAGCTGGGTGAAGTGGCAGCCTTTGGGGGCAGCAATGTGGAGCCC3130.10154688676062187No Hit
TTATCTGGGAGTTAATAATGCATTATATTCCTAAAAATTGCTCAGAGAGTGA3120.10122245581250487No Hit
AACTGCAGGGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGTA3120.10122245581250487No Hit
CATGAGGGGGTGCCCCGAGGTGAGGCGGCGGGGGCGGTGCAGGAGCTGGCGC3110.10089802486438787No Hit
GCTAATGGGTATGACATTGTGATATCAGTGTTTGACTCTCATTGTGTATGTT3110.10089802486438787No Hit
GTTCGCGGGGTAACCTGGTGGAAAAGTAGATTAAGCAAATGCAAAGTGTACA3100.10057359391627085No Hit
TGAAATGGGACTTGTGATATATGATTGCATTAGATAATTTTCTGAAAGTTCC3090.10024916296815385No Hit
AAGTCAGGAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAAT3090.10024916296815385No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)