FastQCFastQC Report
Sat 31 Dec 2022
SRR5849023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186255
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC16410.8810501731497141No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT12880.6915250597299402No Hit
CGAGATGGGGATCTGATAAGGATTCAGGCCTTTGCTTGACTTTCCCAACCTA3370.18093474000697968No Hit
TGCACTTGGGGTATTAGTTTGGCACCAAGGCATGCAGAGATCTATGGTGTCA3290.17663955330058254No Hit
AACAAAGGGAGGGCATTAGATCCAAAGTAATCTTAGCGATTTGAAGCCTAAT3080.16536468819629002No Hit
GAATCCGGGAATCAAGACCAATGCGGGATTGCAGAAGTATATTTAGATTGTT2790.1497946363856004No Hit
AGCTTAGGGAGATCACGGCTGGAGTTATAAATTTGGGAGACAGCATAGATAT2610.14013046629620682No Hit
AAAACTGGGGATCTCCAAATGGGACCCTTCACCCAGCTTCACCTCATGTTAA2540.1363721779281093No Hit
TGAGTAGGGATTATGGGAATTTTATAAGGAAAGAAATTGAGTAACCACTATG2350.1261711095004161No Hit
ACAAGCGGGATACTAAGCTTGGTTTAGATTTTTTGTTCTGCTTTTTTTTTTT2280.1224128211323186No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2260.1213390244557193No Hit
AATCGCGGGATATCTGCAGTTACTTTTCCGCTGAAGTCTTTTTTTTTTTTTT2260.1213390244557193No Hit
GTAGTGGGGGGACGGGGTGACTCCTTTGCATGATGCACTGTCAAACGGACAT2190.1175807360876218No Hit
CAACATGGGATCATTTGCAGGGCTCAGTGCAAAATGAAACAGAGGGACTCTA2130.11435934605782394No Hit
TGCGAGGGGACAGTAAGATAAAGGGAAACATATTTTTAAAGTATTGAAAGAA2120.11382244771952431No Hit
CGTATAGGGCCATGGCTTGATAGCTAATTTCTTTTTATTACTGAATGATATT2110.11328554938122466No Hit
TCACACGGGATAATTTTAAAGGATAAAGAATTTTGTGTTGAGTGTTTTTATC2080.11167485436632572No Hit
CAGTCAGGGGAGGGATGTCTCTGCTGCAGGCTCTGGAAAAAAAAGATGGAGC2070.1111379560280261No Hit
GGTTGGGGGATATGAAAAAATGCTCAACATCACTAATCATGAGAGAAATGTA2050.1100641593514268No Hit
AATGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA2040.10952726101312717No Hit
CGGCCGGGACCCTGCCTTGTCCTGGCAAGCATAGGTTTTCCCAGACCACAAT2020.10845346433652789No Hit
AGCAGGGGGGAAGAAATTCAAAACCTGAACAGACCAATAACAAGTAATGAGA2010.10791656599822824No Hit
GGCAACGGGTGAAGGAACTTGATCCAGCAGAGCACTCCTATGTCTTGATCAG1970.10576897264502967No Hit
AGATGCGGGATGTCCTCTCTATTCTCTCATGAAAGCTGGACATTTTTTTGTT1960.10523207430673003No Hit
TCCCATGGGGATCTCATTGCGGGCAGCACTTTCTCAGACTCACTTATAAGAG1950.10469517596843037No Hit
ATGTAAGGGAGGTTAATCCAGCAACATATATCTGAATGAATTATAATAGGAA1950.10469517596843037No Hit
CCGCCTGGGACACAAATATTTGTAACATATTGTGACCAAATGGGCCTCAAAG1940.10415827763013075No Hit
CCTCAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1930.10362137929183109No Hit
ACATGAGGGATATTCTGCTTTTTTTGTCTTAGATAGAAATAAACAATTACAT1920.10308448095353145No Hit
GTTCAGGGGAGGAGAAAGTGTTTAACATTCCACCCCACATCATCGCGCAATC1920.10308448095353145No Hit
TGCGTGGGGGAATCTTGCCTTGATATTGATGGTTTCTAATAGACCAGGGTGG1910.10254758261523181No Hit
AACCAGGGGAACCTTGTCAGATGTTAACTTCTAAGTTCGGTTTGGGATTTTT1900.10201068427693216No Hit
CTACCAGGGGACGCTCTTCCGATCTATACAAGGGGAAATTGGACCCTTATCT1900.10201068427693216No Hit
TCTCACGGGGGGGCCTCTGTCTTTTGTGGTGACCCCTCAGGGGTTTCATTGT1890.10147378593863253No Hit
GCTTTGGGGATTGGTGTATAGGAATGCTTGTGATTTTTGCACATTGATTTTG1890.10147378593863253No Hit
ATCAATGGGAGTCTATGACTTATTTCTGCGAAAGCCCATGAAAGTGAAAACT1890.10147378593863253No Hit
CCGAGAGGGACGCGGCCTACAACGTGCTGGACGACCCGGAGCTCCGACAAGG1880.10093688760033287No Hit
TAGCTGGGACTGAGGTAAACTGGAATCTCTTGTTTTCAGGAAAGATTGTTTT1880.10093688760033287No Hit
CATTTGGGCACTTTCCAGAAATGGAGATATGAAAAGATTCTCTGGAATCCTT1870.10039998926203322No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)