FastQCFastQC Report
Sat 31 Dec 2022
SRR5849022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185722
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC15630.8415804266592003No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT12840.6913558975242566No Hit
GAATCCGGGAATCAAGACCAATGCGGGATTGCAGAAGTATATTTAGATTGTT3100.1669161434832707No Hit
CGAGATGGGGATCTGATAAGGATTCAGGCCTTTGCTTGACTTTCCCAACCTA3060.16476238679316396No Hit
AACAAAGGGAGGGCATTAGATCCAAAGTAATCTTAGCGATTTGAAGCCTAAT3050.1642239476206373No Hit
TGCACTTGGGGTATTAGTTTGGCACCAAGGCATGCAGAGATCTATGGTGTCA3040.1636855084481106No Hit
TGAGTAGGGATTATGGGAATTTTATAAGGAAAGAAATTGAGTAACCACTATG2860.15399360334263038No Hit
AGCTTAGGGAGATCACGGCTGGAGTTATAAATTTGGGAGACAGCATAGATAT2850.15345516417010371No Hit
AAAACTGGGGATCTCCAAATGGGACCCTTCACCCAGCTTCACCTCATGTTAA2530.13622511064924997No Hit
GCTTTGGGGATTGGTGTATAGGAATGCTTGTGATTTTTGCACATTGATTTTG2370.12761008388882308No Hit
GCCTCCGGGGATTACATGGATACTTCTAAAAGCTAAAGCCTTGTTGCCTTCT2280.12276413133608297No Hit
CGGCCGGGACCCTGCCTTGTCCTGGCAAGCATAGGTTTTCCCAGACCACAAT2230.12007193547344958No Hit
TCACACGGGATAATTTTAAAGGATAAAGAATTTTGTGTTGAGTGTTTTTATC2230.12007193547344958No Hit
AATCGCGGGATATCTGCAGTTACTTTTCCGCTGAAGTCTTTTTTTTTTTTTT2170.1168413004382895No Hit
TCTAATGGGGATCTGCAGTGGGACTTTAAAAAAATAACTTTATCTAGGATTG2120.1141491045756561No Hit
TGCGAGGGGACAGTAAGATAAAGGGAAACATATTTTTAAAGTATTGAAAGAA2120.1141491045756561No Hit
GTTACTGGGGAAAAACCAATAAGCTGGTGACTCATGAATACACACGAGGAAA2110.11361066540312942No Hit
AGCAGGGGGGAAGAAATTCAAAACCTGAACAGACCAATAACAAGTAATGAGA2090.11253378705807604No Hit
ACTTAGGGGGAAAAAAATGTAAAGCCAGAAAGGAGAATATATGTGTTGAAGG2090.11253378705807604No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2080.11199534788554937No Hit
GTTCAGGGGAGGAGAAAGTGTTTAACATTCCACCCCACATCATCGCGCAATC2060.110918469540496No Hit
GGCAACGGGTGAAGGAACTTGATCCAGCAGAGCACTCCTATGTCTTGATCAG2050.11038003036796933No Hit
GTAGTGGGGTACGCAGGGACATTTGAGGATCGTCACCAAGATGAAGGAATGA2050.11038003036796933No Hit
GTAGTGGGGGGACGGGGTGACTCCTTTGCATGATGCACTGTCAAACGGACAT2040.10984159119544264No Hit
TGATTGGGGTAACGGACTGCTCTTTATCCTGCACCTTTTTCATATTTCTTCT2030.10930315202291596No Hit
CGTATAGGGCCATGGCTTGATAGCTAATTTCTTTTTATTACTGAATGATATT2020.10876471285038929No Hit
CGTGACGGGAACATTGTAAGATTGAGAGTAGGAATTCATTGAGATTTTAATG2000.10768783450533592No Hit
GCAAAGGGCTATTATTACCTGGTAGGAGTACAATACATAGAATTATCTTCTA1980.10661095616028256No Hit
ACAGTAGGGTGATTTAGATAGAATAAAAGTGTCACAATTTTAACAGCTTACA1970.10607251698775588No Hit
GGTGATGGGAGCAAGCAGGTTACCAGCCAGTCTTGTCTGGTCAACAGGGATT1960.10553407781522921No Hit
GGTTGGGGGATATGAAAAAATGCTCAACATCACTAATCATGAGAGAAATGTA1960.10553407781522921No Hit
AATGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1960.10553407781522921No Hit
TCCCATGGGGATCTCATTGCGGGCAGCACTTTCTCAGACTCACTTATAAGAG1950.10499563864270253No Hit
TAAAGAGGGATGAGCATCCAGTGTTATTTAGCTTTTAAAACATATTATTTGT1930.10391876029764917No Hit
CTTACGGGGGAACTGATCCTCTGTCCCTGCAACCGTTGTTTAAGGATGCTAT1920.10338032112512249No Hit
TTCGAAGGGAGCTTGTTTAGGGATGAAGTAGTAATTTCAATAAAAGAGTGCC1920.10338032112512249No Hit
CCAAAGGGATGACTTTACTCTGGATGACTAAAACAAAGGGAAGAACTTTTTA1910.1028418819525958No Hit
GGCCCTGGGAGTATTGAAATTTCTAACCCATACCCCTGTGAAAGAAAATCTA1910.1028418819525958No Hit
CTAACCGGGACAGACAGCATCACACTTTGCAGCATTCACACCCACATATTGA1910.1028418819525958No Hit
TGCGTGGGGGAATCTTGCCTTGATATTGATGGTTTCTAATAGACCAGGGTGG1880.10122656443501576No Hit
ATGGCCGGGTAAGCCCTTCTCTACTTTGGGCTTCCTATTCAGCTGCTGTAGG1870.10068812526248909No Hit
GAAACTGGGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTG1870.10068812526248909No Hit
CGGCTAGGGCGATCTTGTTCGGGCATTGATAATATAACCTTTTTTTGTTTTT1870.10068812526248909No Hit
TTGCCAGGGATCAAAATTAAGTTTACTTGTATACGGAACAACCACAAGAAAT1870.10068812526248909No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)