FastQCFastQC Report
Sat 31 Dec 2022
SRR5849021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186078
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC16060.8630789238921313No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT13000.6986317565751996No Hit
GAATCCGGGAATCAAGACCAATGCGGGATTGCAGAAGTATATTTAGATTGTT3460.18594352905770697No Hit
TGCACTTGGGGTATTAGTTTGGCACCAAGGCATGCAGAGATCTATGGTGTCA3100.16659680349100914No Hit
CGAGATGGGGATCTGATAAGGATTCAGGCCTTTGCTTGACTTTCCCAACCTA2910.15638603166414083No Hit
AACAAAGGGAGGGCATTAGATCCAAAGTAATCTTAGCGATTTGAAGCCTAAT2890.15531121357710206No Hit
AGCTTAGGGAGATCACGGCTGGAGTTATAAATTTGGGAGACAGCATAGATAT2880.1547738045335827No Hit
TGAGTAGGGATTATGGGAATTTTATAAGGAAAGAAATTGAGTAACCACTATG2690.1445630327067144No Hit
AAAACTGGGGATCTCCAAATGGGACCCTTCACCCAGCTTCACCTCATGTTAA2520.13542707896688486No Hit
GTAGTGGGGGGACGGGGTGACTCCTTTGCATGATGCACTGTCAAACGGACAT2460.13220262470576855No Hit
GTTACTGGGGAAAAACCAATAAGCTGGTGACTCATGAATACACACGAGGAAA2260.12145444383538087No Hit
TGCGAGGGGACAGTAAGATAAAGGGAAACATATTTTTAAAGTATTGAAAGAA2260.12145444383538087No Hit
ACAAGCGGGATACTAAGCTTGGTTTAGATTTTTTGTTCTGCTTTTTTTTTTT2190.11769258053074518No Hit
ACTTAGGGGGAAAAAAATGTAAAGCCAGAAAGGAGAATATATGTGTTGAAGG2120.11393071722610947No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2070.11124367200851255No Hit
AGCAGGGGGGAAGAAATTCAAAACCTGAACAGACCAATAACAAGTAATGAGA2060.11070626296499318No Hit
ATGTAAGGGAGGTTAATCCAGCAACATATATCTGAATGAATTATAATAGGAA2060.11070626296499318No Hit
GGTTGGGGGATATGAAAAAATGCTCAACATCACTAATCATGAGAGAAATGTA2060.11070626296499318No Hit
GCTTTGGGGATTGGTGTATAGGAATGCTTGTGATTTTTGCACATTGATTTTG2030.10909403583443501No Hit
TAGCTGGGACTGAGGTAAACTGGAATCTCTTGTTTTCAGGAAAGATTGTTTT2010.10801921774739626No Hit
GTAGTGGGGTACGCAGGGACATTTGAGGATCGTCACCAAGATGAAGGAATGA1980.1064069906168381No Hit
CAGTCAGGGGAGGGATGTCTCTGCTGCAGGCTCTGGAAAAAAAAGATGGAGC1970.1058695815733187No Hit
GTATGCGGGGATGGGGCACAGCCCGCCTTTGAACAATCATGCTTCAGAAATC1960.10533217252979934No Hit
CAACATGGGATCATTTGCAGGGCTCAGTGCAAAATGAAACAGAGGGACTCTA1960.10533217252979934No Hit
GCCTCCGGGGATTACATGGATACTTCTAAAAGCTAAAGCCTTGTTGCCTTCT1950.10479476348627995No Hit
CCGAGAGGGACGCGGCCTACAACGTGCTGGACGACCCGGAGCTCCGACAAGG1940.10425735444276056No Hit
ACTACAGGGAATATAAATCAAGTGAATCTGTGTCTCTGTTACAAAAATAGAA1930.10371994539924118No Hit
CCAAAGGGATGACTTTACTCTGGATGACTAAAACAAAGGGAAGAACTTTTTA1920.10318253635572179No Hit
ACACGCGGGATTCCATATTGATTGTTGTTGTGTTGTGTGACAGACAATAGAG1920.10318253635572179No Hit
ACAGTAGGGTGATTTAGATAGAATAAAAGTGTCACAATTTTAACAGCTTACA1910.10264512731220242No Hit
GAAACTGGGATTCCTAACAAGTCAGTCATTTGTGAACTTAGTGGACTTTTTG1890.10157030922516364No Hit
CGGCCGGGACCCTGCCTTGTCCTGGCAAGCATAGGTTTTCCCAGACCACAAT1890.10157030922516364No Hit
GGCCCTGGGAGTATTGAAATTTCTAACCCATACCCCTGTGAAAGAAAATCTA1890.10157030922516364No Hit
TAAAGAGGGATGAGCATCCAGTGTTATTTAGCTTTTAAAACATATTATTTGT1880.10103290018164425No Hit
TCCCATGGGGATCTCATTGCGGGCAGCACTTTCTCAGACTCACTTATAAGAG1870.10049549113812487No Hit
GTTGTGGGAGTTTTTACTTTATCTGTTTACTTATCTCTTTGTCTATTAGAAA1870.10049549113812487No Hit
CGCAGGGGCACCCAATACAGGAGCACCCAGATTCATAAAGCAAGTCCTGAGT1870.10049549113812487No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)