FastQCFastQC Report
Sat 31 Dec 2022
SRR5849020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80409
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC8971.1155467671529307No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT8371.0409282542998917No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2100.26116479498563594No Hit
GAGCTCGGGGGTTTAGCAGTATTCCCAATGATAAGCAAAACCTATTATTGAG2040.25370294370033203No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1320.16416072827668543No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1290.1604298026340335No Hit
TCATTAGGGCAATTCAACAAACATTATTCAACATCCAGTTGAATTTGCAAGG1110.13804424877812185No Hit
GTTATTGGGCATTTAAAGTTCACCTTATCTGGGGAGGCAGAAGGAATTTTCA1100.1368006068972379No Hit
TCAACAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC1070.13306968125458593No Hit
CAGCCGGGGGGAGAAACTAGTGATAGAGAAGCAAATGTAAAATATTAACAAT1010.12560782996928205No Hit
GTGTGGGGAACAGACAAGATTCCTGGGGGCTCCCGATGAGCAGGAACGTGGA920.11441505304132622No Hit
TACGTTGGGAGAGTTGTTGAGACTTCATTTTCTAAGCAATATATGTTTACTC890.11068412739867427No Hit
CACTAAGGGTCTACAAGCGGGATGCAAAGGGCCGTGGCCAACAGGGTGGAGC880.10944048551779029No Hit
CAGCACGGGATCTTCTGAACCTTCTCAGTTAAAACCGGCATAATATATAAAA850.10570955987513836No Hit
TGCTGACGGGAACCATACACCTGTAAGGAATGTGGGAAGGCTTTAACTATTC840.10446591799425438No Hit
ATTGAGGGGGAAAGTACCCAGCAGCAAAGAAGGTTCTCACCAGATGCAGCTC830.1032222761133704No Hit
TTTCCAGGGACTTCCTCCTATGAGTAGGACCTGAAGTCTCCTCCCACAACTC810.10073499235160244No Hit
ATCTCCGGGTGGCTGCTGTTACAGTGAGTTTCTTTTTGTGTTGGAAAGATTC810.10073499235160244No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)