FastQCFastQC Report
Sat 31 Dec 2022
SRR5849019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80516
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC8881.1028863828307418No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT8261.0258830542997663No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2160.2682696606885588No Hit
GAGCTCGGGGGTTTAGCAGTATTCCCAATGATAAGCAAAACCTATTATTGAG1720.21362213721496348No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1500.18629837547816583No Hit
CAGCCGGGGGGAGAAACTAGTGATAGAGAAGCAAATGTAAAATATTAACAAT1100.13661880868398826No Hit
GTAACGGGGATGCCAAAATGAATTTCAAATGAGTTTAAAAGTTAAATGAAAA1100.13661880868398826No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1050.1304088628347161No Hit
CCAACATGCATCCCACACACATACAAGTTTAATGCACAGCAGAACTTGAAAG1050.1304088628347161No Hit
GTTATTGGGCATTTAAAGTTCACCTTATCTGGGGAGGCAGAAGGAATTTTCA1040.12916687366486163No Hit
TCAACAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC980.12171493864573502No Hit
TACGTTGGGAGAGTTGTTGAGACTTCATTTTCTAAGCAATATATGTTTACTC980.12171493864573502No Hit
TTCCCTGGGGTTGACTGATGGGATCTTTATGCTGCTGAGCCATTAGCAAGAT970.12047294947588057No Hit
GTGTGGGGAACAGACAAGATTCCTGGGGGCTCCCGATGAGCAGGAACGTGGA950.11798897113617168No Hit
AATAAAGGGAGAAAGATCCACCGGATCATTCAGCAGGACTGCCAGAAACCAA930.11550499279646281No Hit
CCCCTCGGGGCGGAAGGTTCGGGAGGCTTCTGGAAAAAGCGCCGCGCGCTGG910.11302101445675393No Hit
CTTTACGTGGGGCATGCGTCTTTATAGTAGAATGATTTATAATCCTTTGAGT900.11177902528689951No Hit
CACTAAGGGTCTACAAGCGGGATGCAAAGGGCCGTGGCCAACAGGGTGGAGC900.11177902528689951No Hit
GCTGTTGGGATTTATTTAATAGATTATTTTCTAACTTCTTCCAGTACTACAT890.11053703611704506No Hit
TCATTAGGGCAATTCAACAAACATTATTCAACATCCAGTTGAATTTGCAAGG880.10929504694719061No Hit
CTATGTGAGGCGATCTCGCGCAGGGAATTGGCATTTGACAAGGCTGCCAAAA860.10681106860748174No Hit
TTTCCAGGGACTTCCTCCTATGAGTAGGACCTGAAGTCTCCTCCCACAACTC850.1055690794376273No Hit
TGGCATGGGGGAGCGCGCGAGGTGAGGAGTTGTGCAGGGTATGGGGAAAGGA830.10308510109791844No Hit
CTTCTGAGGGGTACCCAACAACTGCAGGGCCTTGCTGTGACAATAAGCAGAG830.10308510109791844No Hit
ACTCTGGGGAGATTTCCTGTGACATAGAAAACTCTTCTTGTTTAGATGCGCT820.10184311192806399No Hit
CAGCACGGGATCTTCTGAACCTTCTCAGTTAAAACCGGCATAATATATAAAA810.10060112275820955No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)