FastQCFastQC Report
Sat 31 Dec 2022
SRR5849018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80262
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC8111.0104408063591737No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT7960.9917520121601754No Hit
GAGCTCGGGGGTTTAGCAGTATTCCCAATGATAAGCAAAACCTATTATTGAG2370.2952829483441728No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1880.23423288729411176No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1240.15449403204505244No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1000.1245919613266552No Hit
CAGCCGGGGGGAGAAACTAGTGATAGAGAAGCAAATGTAAAATATTAACAAT940.11711644364705588No Hit
GTGTGGGGAACAGACAAGATTCCTGGGGGCTCCCGATGAGCAGGAACGTGGA940.11711644364705588No Hit
GAAGCGGGCTACTTTTGGATTAATTGAATATTTTTTAGTGTTTTATTAATTG930.11587052403378933No Hit
GTTATTGGGCATTTAAAGTTCACCTTATCTGGGGAGGCAGAAGGAATTTTCA920.11462460442052279No Hit
TCAACAGGGAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTATC910.11337868480725624No Hit
GTAGTGGGGCAAAACAGAGTGACACTATTAATATCAAATACAGTAGACTCCA900.1121327651939897No Hit
CAGCTTGGGAGCCATAATAATGAATACCAAAATCCACCTTTTATTAGAGATT860.10714908674092348No Hit
CAGCACGGGATCTTCTGAACCTTCTCAGTTAAAACCGGCATAATATATAAAA850.10590316712765693No Hit
ACTCTGGGGAGATTTCCTGTGACATAGAAAACTCTTCTTGTTTAGATGCGCT840.10465724751439037No Hit
TTTCCAGGGACTTCCTCCTATGAGTAGGACCTGAAGTCTCCTCCCACAACTC830.10341132790112383No Hit
TGGGGCGGGCTTTAGATTTATGTTAACCACCTTGTTTCATTTGGTTTTAGGA830.10341132790112383No Hit
CTATGTGAGGCGATCTCGCGCAGGGAATTGGCATTTGACAAGGCTGCCAAAA830.10341132790112383No Hit
TACGTTGGGAGAGTTGTTGAGACTTCATTTTCTAAGCAATATATGTTTACTC820.10216540828785727No Hit
TTAGTTGGGGTACCCAGTGCCCTAGAATGAATTCTGTGGTGATTGGGGTTAT810.1009194886745907No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)