FastQCFastQC Report
Sat 31 Dec 2022
SRR5849014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76740
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC12581.63930153765963No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT11601.5115976022934585No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2310.30101641907740423No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1400.18243419338024497No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1060.13812874641647122No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC1060.13812874641647122No Hit
TCTGCTGGGAGTTTAAACTTTTTTATATTCAGTTTTGCTTTTCAAAATATAC970.12640083398488403No Hit
TTGGTTGGGAGAAGCACTAACAATGCCAGTGACCATGTAACATGGATTTAAG920.11988532707844671No Hit
TCAGGGGAGTTTCTGTATTTCATATGCCAAAGCTATGTTGATTTAAATACTG910.11858222569715925No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC900.11727912431587179No Hit
CCACTGGGGGCAGATGCCAAATGACCGGTACAAAAGGGAATGCAAAAAACCC870.11336982017200938No Hit
GAATAAGGGATTTTATATGTGTGTGATTTCATGTATTTAATCATTTTCCACT830.10815741464685953No Hit
AATGTAGGGCAGAAAAAAACCCCAACAAACAGATGAATGGATAAAGAAAATG830.10815741464685953No Hit
TTTAATGGGGCATCTCTGCTGGTGCTCCACTTTGTCACATACAGTTTTTGTT830.10815741464685953No Hit
GCACATGGGGTACTTATCAAAATTAATTGTCCCCACAAAAAACTTGTGTACA780.10164190774042221No Hit
TGAGCGTGGGGAAAACAAGACAACTGAAATGAGGTAACTTGCAGCAATTGAA770.10033880635913475No Hit
AAACGCGGGAGAGCAAGTTACATCAAAGGATATGTGACCAGACTCCCTCCAG770.10033880635913475No Hit
GTAGAGGGACTTCAGCCAGTCCCTCCCAAGTTTGGGAGAATCAAGGCCCCTT770.10033880635913475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)