FastQCFastQC Report
Sat 31 Dec 2022
SRR5849013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75420
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC12141.609652612039247No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT11191.4836913285600637No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1990.26385574118271016No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1380.18297533810660302No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1120.1485017236807213No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC990.13126491646778043No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC950.12596128347918326No Hit
TTGGTTGGGAGAAGCACTAACAATGCCAGTGACCATGTAACATGGATTTAAG940.12463537523203394No Hit
TATGAAGGGAAGCTATTTTTTATTATATTTTGCCCTTGCAGTTGGCGTTTGA900.11933174224343676No Hit
TCTGCTGGGAGTTTAAACTTTTTTATATTCAGTTTTGCTTTTCAAAATATAC880.11667992574913816No Hit
TCAGGGGAGTTTCTGTATTTCATATGCCAAAGCTATGTTGATTTAAATACTG850.11270220100769027No Hit
CGTTGAGGGGGTCTTAAGGTATTTTAAGGAAAATTAATGAGGAGCTTTTTGT840.11137629276054097No Hit
CTCACGGGGGTTGTTTCCTTTTCTCCTTAAGCTTTATTCGCAACTATATCCT810.10739856801909309No Hit
GCCAGGCGGGGTGTCTGGAAAAGCGTAGGCATCCGGAGGCTTTGGAGGGGAG790.10474675152479448No Hit
CAGAGAGGGACAGACAATCCCAACATCACACACTGTTCCCACATCACACGAA770.10209493503049588No Hit
TATGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA760.10076902678334661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)