FastQCFastQC Report
Sat 31 Dec 2022
SRR5849012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75548
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC11241.4877958384073702No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT11191.4811775295176577No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC2420.320326150262085No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1370.1813416635781225No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1130.1495737809075025No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC1010.1336898395721925No Hit
TCAGGGGAGTTTCTGTATTTCATATGCCAAAGCTATGTTGATTTAAATACTG1000.13236617779425003No Hit
CTTCATGGGTAAACAAACTTGAATTGTCACTTACCAGGTCTTACAAGATTAG990.1310425160163075No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC950.1257478689045375No Hit
GATTGGGGTCACTGTCAACTGATTTCGGACTTTGACATATAATATTTTAAAA830.10986392756922753No Hit
AACGATGGGATAAGATGGTTTAATTAACTGTCTGGTTTCTTTTGGTTGGGTA820.108540265791285No Hit
GCCAGGCGGGGTGTCTGGAAAAGCGTAGGCATCCGGAGGCTTTGGAGGGGAG810.1072166040133425No Hit
TCTGCTGGGAGTTTAAACTTTTTTATATTCAGTTTTGCTTTTCAAAATATAC810.1072166040133425No Hit
AATGTAGGGCAGAAAAAAACCCCAACAAACAGATGAATGGATAAAGAAAATG790.10456928045745752No Hit
ACCACTGGGAGAATTACAGTTAGTAGTAAGAAAAAATAATTTAAGCTATCAA780.10324561867951501No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCTTATAC770.10192195690157252No Hit
ATTTCTGGGGAAAAGAACAATAGCAACCATGTCTAACTAATACTTCTTAATC760.10059829512363001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)