FastQCFastQC Report
Sat 31 Dec 2022
SRR5849002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35189
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC9662.7451760493335984No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT9542.7110744835033675No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1610.45752934155559977No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1210.34385745545482965No Hit
AGAGGCGGGGAGAAGAATATTAGAGAAATACTGACCAAAGGTCAAAGATTTC1040.29554690386200233No Hit
TTTCTTGGGATATCCAAGGAATCATTGCCAAATTCAGTGTCATGAAGTTTTC1040.29554690386200233No Hit
AACTCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1010.28702151240444457No Hit
GCTAAAGGGAGAATATATGTTGGACCTCTGAAAAGTTGTAAGGGGCCAATTC940.2671289323368098No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA800.22734377220154026No Hit
AATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA790.224501975049021No Hit
GACAAGGGATGCCACTGCACTTCAACTTGGGCAACAGAGCAAGACCCTGTCT770.2188183807439825No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC760.21597658359146324No Hit
GTAACAGGGGCTGATAAATGTATCAGAAACTCTTTCATCATTTTCTGTCCCC760.21597658359146324No Hit
CATTTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC720.20460939498138622No Hit
GTCTTAGGGGATGCAAGGATGTAGTTTTGTACTGAAAGAATAAACACTGACT710.20176759782886697No Hit
ATCGGGGTATCTACGCCTGGGTAGTTCAGTACTCCAAATTATTGGGGACCTT700.1989258006763477No Hit
CTTATGGGGATATTTCCCAATCATTGTCTTCATTCCACTACAAAGTCACCTC690.19608400352382846No Hit
ATCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA680.1932422063713092No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATACTGTCTCTTATACAC680.1932422063713092No Hit
TCAACCGGGAATTATAGCCTTTTAAGAAAATAATCAAAGAAGTAAGTTTCAT680.1932422063713092No Hit
CAGGCAGGGAGTCACTCTGGTTTTGTAATGATTTGCCTTTTAGGCCTCAGGC670.19040040921878995No Hit
GCATTTGGGGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAA640.18187501776123222No Hit
ATGCATGGGAGTGGGAGATTTCCACACTCCAATGACAGTATTAGACAGATGA640.18187501776123222No Hit
ATTCTGGGGATTCATCTCTGTCTAGAGTATTTCATGTGTGTATGTGTTGCTT630.17903322060871296No Hit
TAGATGGGAATCATAATAATAATTTAAAAGAGTGATGATACCAAGTGTTGGT630.17903322060871296No Hit
TCCTGGGGGAAGGCTGTGATTATGTTTGATATTCACCTGGATTTTAATACAA610.17334962630367445No Hit
GTTCTGGGGTCAGAGGGCCATGAGGTTGCATTTTTTAAATTGAGGTAAAATT600.1705078291511552No Hit
ATTGTTGGGATACATGTGCAAGGGAGACAAAAGAACTCAACAGAATTTTACT600.1705078291511552No Hit
CTGTTTGGGAAGACATTTTTCCTGATATGTAGAGCAGTCTGTTGGCAAAAAT590.16766603199863594No Hit
TGCAGAGGGAAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGG580.1648242348461167No Hit
ACTACGGGAGTGTGGGAAAAGTGTTTCCATTCTGGGAAAAGCCCAAACCGAA580.1648242348461167No Hit
CTCACATGGGGAAAAGATAAATTGACAAACCATTAGCCAGACTAAGAAAAAA570.16198243769359744No Hit
TGGAAGGGAGATGGGTTAGATAAAGATGAATATACATTTTCACCAGGCAAAT560.15914064054107818No Hit
TAGCGAGGGGAGACAGTTTTATCATCCACATTGCTGACCTGGAAGATGCATC560.15914064054107818No Hit
TCAGCGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAG550.15629884338855893No Hit
CATCATGGGATTCTGTAAAAACTCTTTATAAAAAGTTACTATTCGTATCTGT550.15629884338855893No Hit
GTAACTGGGACTACAGCGGAGGAGATGCCCATGAATGTGGCTGACCTTATTT550.15629884338855893No Hit
ACAGTTGGGCGCTTGTGGTCCTCCAACAACTAAATCTCATCCCCCATGAGGT520.14777345193100117No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATAACTCGTATGC520.14777345193100117No Hit
TTTAACGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT520.14777345193100117No Hit
GCTTTAGGGAGATGGACAAGGAAGCAATTTGAGTAATTTTGTGAAAATTTCA510.1449316547784819No Hit
AGTAATGGGACTGCGGGGATAGTTGATGTAACATTGTAGAAGCAGCATAGGA510.1449316547784819No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT500.14208985762596266No Hit
ACAAGAGGGCGTTTCTTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCA500.14208985762596266No Hit
CGGACCGGGGCTTTATGACTGGGTGTGTGTTGTGTGTATGTAATACATAATG500.14208985762596266No Hit
GCGCCGGGAGTAAATGAATATATGTTTTGATTTAACACATCTTTACAATTAA500.14208985762596266No Hit
GATGCTGGGATGGTGCCTGGCCAAACGTTTTTATTACAAATATTACATTAAA500.14208985762596266No Hit
GTCAAAGGGGCCTGTGTTGTTGCTATGTGTATTTGAAAGTAATATCTGCATT480.13640626332092415No Hit
AATTGTGGGATGGGGACCTGGAACCTGCCCCTTATCCAACCAAGTAATTAGG480.13640626332092415No Hit
AGCACAGGGATAATTTGTGATCCTGGGTTTTCCTTCCTTAAAAATACTTCTT480.13640626332092415No Hit
ATTCCGGGGAGACTCAGGCTTACCTTGCACAAAAATAAATTCCTTTTCATAT480.13640626332092415No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCT470.1335644661684049No Hit
TATAGCGGGCTTTGTGATCTCATAGCTTGCTACGCTTTTATATCTTATGGCT470.1335644661684049No Hit
CTGAGCGGGAGGTAGCTGGGACCTAGGCTATCTTACCATGAAGGTTGTTTTG470.1335644661684049No Hit
ACGAGGGAATTCTGAAAATAAGAACCTGTTAATAGGTTGGAAAGCTGTGTAC460.13072266901588564No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCTTATAC460.13072266901588564No Hit
GGGCAGGGGATGAGATTGATTTGTTGTGAAGTTATTGGCTATAATGTCTTTG460.13072266901588564No Hit
TTATGGGGACCCCTAAAATTTTAAGAACTGTACTTTATAAAAATGTGCAATT460.13072266901588564No Hit
GGCCAGGGGATGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGT460.13072266901588564No Hit
ATACATGGGGGTTGAGCTGATGTCAATTGGTTCTTGTCTTGAGTCGACTCAA450.12788087186336639No Hit
GTGCTGAGGGCAAATAAATCAATTTAAGACCTATTGCTTCTGACAATGATTT440.12503907471084713No Hit
CGGTTCGGGAGGTGAACAATCAACTAGTATTTGTTGAGCTCCTATTTGCCCA440.12503907471084713No Hit
CGTTCTGGGAGAGTATTGCAGGCAATAATACGACTAAAATTCTAATACTTTT430.12219727755832788No Hit
GTTACAGGGAGAGCTTAAAAGTTTATAAGCATTAAAAACACATACATGTATT430.12219727755832788No Hit
TGTTTAGGGAGCATGATTTTTTTGCACATGTAGAAATTTTTTAAAAGAAAGA420.11935548040580862No Hit
TAATGCGGGACAGTCATAAACTGTTCAAGGTAACCATAACAAACTAGGTGTT420.11935548040580862No Hit
AGCACCGGGAGTTCTGGCCATTCTGAGCCTGCTTTTGTGATTGCTCATCCAT420.11935548040580862No Hit
TTGAAGCGGGAGGGTGTTCTTTTTTATGAAGAAAAATTTGAAAATGATAAAA420.11935548040580862No Hit
GTGGCAGGGAGAGCCTTGAGTAAACATCTTTTCAGGATTTTGTGTGTTGAAA410.11651368325328937No Hit
CACTCAGGGACCCTGGGTACTGAACAAAAAAACCCAAATTACACTCCTTATT410.11651368325328937No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATAT400.11367188610077013No Hit
GTACCTGGGAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAATTAATA400.11367188610077013No Hit
GACCAGGGGAATCCCTTAAGCATTTGATGAAACACTCTTTAGTGCTATATGC390.11083008894825087No Hit
TTTCTAGGGAGAACTAGTAGTACTAGTAGTACTGTCTCTAGCTCTTCATACA390.11083008894825087No Hit
GTATGTGGGATTTTTGTCTATGGTAGTTATTTCACAAAGCCAAGCGAGTTAC390.11083008894825087No Hit
ACTTAGGGTCACCTTAAAAAAACAGAATGAGTCATAAGGAGTCAGACATGCC390.11083008894825087No Hit
TGCTACGGGCAAATCACTCAGCAGCTGTTTTTGTTTGTTTGTTTGTTTGTTT380.10798829179573162No Hit
GTGCGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA380.10798829179573162No Hit
TTAGCAGGGATGTCAAGTGCAGTTGTGAGGACTGTGTGGGTGAACGAAAATG380.10798829179573162No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTC370.10514649464321237No Hit
AACTTAGGGATTCTGCACATGTATCCCAGAACTTAAAGTAAAATAAAATAAT370.10514649464321237No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATACTGTCTCTTA370.10514649464321237No Hit
CATCATGGGGATAAAAGGGTACATCCATCAAGAAAATATCACCGTTATCAAC360.10230469749069311No Hit
CATTTCATTTCTTAGGCATTTTCTCTCTTATAAAGTTTCCTGGATTTATAAA360.10230469749069311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)